(Circular) (Six-base) MAPSORT of: pFN21AE3768.seq Check: 668 from: 1 to: 7567 pFN21AE3768 7567 bp With 182 enzymes: SgfI * March 28, 2012 12:10 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2416 2599 3330 2416 Size: 183 731 6653 Fragments arranged by size: 6653 731 183 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5377 278 Size: 53 83 186 936 3841 2468 Fragments arranged by size: 3841 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4910 1937 Size: 2973 4594 Acc65I G'GTAC_C Cuts at: 4888 4888 Size: 7567 AclI AA'CG_TT Cuts at: 1563 5694 6067 1563 Size: 4131 373 3063 Fragments arranged by size: 4131 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3752 5630 7014 7282 1632 Size: 2120 1878 1384 268 1917 Fragments arranged by size: 2120 1917 1878 1384 268 AfeI AGC'GCT Cuts at: 2791 3242 2791 Size: 451 7116 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7370 AflIII A'CryG_T Cuts at: 1203 3162 4277 6466 7443 1203 Size: 1959 1115 2189 977 1327 Fragments arranged by size: 2189 1959 1327 1115 977 AgeI A'CCGG_T Cuts at: 6398 6398 Size: 7567 AhdI GACnn_n'nnGTC Cuts at: 2674 6296 2674 Size: 3622 3945 AleI CACnn'nnGTG Cuts at: 1166 4241 1166 Size: 3075 4492 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3410 3893 1184 Size: 2226 483 4858 Fragments arranged by size: 4858 2226 483 AlwNI CAG_nnn'CTG Cuts at: 3280 4594 5310 6882 3280 Size: 1314 716 1572 3965 Fragments arranged by size: 3965 1572 1314 716 ApaI G_GGCC'C Cuts at: 2061 2350 3633 3687 4211 4776 4855 2061 Size: 289 1283 54 524 565 79 4773 Fragments arranged by size: 4773 1283 565 524 289 79 54 ApaLI G'TGCA_C Cuts at: 2544 5624 6780 2544 Size: 3080 1156 3331 Fragments arranged by size: 3331 3080 1156 ApoI r'AATT_y Cuts at: 1493 4876 5102 5254 7363 1493 Size: 3383 226 152 2109 1697 Fragments arranged by size: 3383 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6121 160 Size: 872 5089 1606 Fragments arranged by size: 5089 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7567 AvaI C'yCGr_G Cuts at: 1870 1943 2535 2616 2990 3354 3940 4523 4657 Size: 73 592 81 374 364 586 583 134 Cuts at: 4657 4892 1870 Size: 235 4545 Fragments arranged by size: 4545 592 586 583 374 364 235 134 81 73 BamHI G'GATC_C Cuts at: 3494 4897 5264 3494 Size: 1403 367 5797 Fragments arranged by size: 5797 1403 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2240 2489 2575 2815 3952 Size: 325 217 627 453 249 86 240 1137 Cuts at: 3952 4001 4067 4225 4476 4888 6343 618 Size: 49 66 158 251 412 1455 1842 Fragments arranged by size: 1842 1455 1137 627 453 412 325 251 249 240 217 158 86 66 49 BanII G_rGCy'C Cuts at: 1391 2061 2350 2458 2997 3633 3687 3936 4211 Size: 670 289 108 539 636 54 249 275 Cuts at: 4211 4530 4769 4776 4855 4886 1391 Size: 319 239 7 79 31 4072 Fragments arranged by size: 4072 670 636 539 319 289 275 249 239 108 79 54 31 7 BbeI G_GCGC'C Cuts at: 2244 2493 2819 3956 4005 4071 4229 2244 Size: 249 326 1137 49 66 158 5582 Fragments arranged by size: 5582 1137 326 249 158 66 49 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2206 3144 4206 928 Size: 971 307 938 1062 4289 Fragments arranged by size: 4289 1062 971 938 307 BbvCI CC'TCA_GC Cuts at: 3542 4166 3542 Size: 624 6943 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2981 3015 5758 5792 1903 Size: 34 1044 34 2743 34 3678 Fragments arranged by size: 3678 2743 1044 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5459 6669 5459 Size: 1210 6357 BclI T'GATC_A Cuts at: 1548 1548 Size: 7567 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2208 2813 4076 Size: 107 122 71 631 605 536 605 1263 Cuts at: 4076 4316 6178 136 Size: 240 1862 1525 Fragments arranged by size: 1862 1525 1263 631 605 605 536 240 122 107 71 BglII A'GATC_T Cuts at: 7562 7562 Size: 7567 BlpI GC'TnA_GC Cuts at: 3338 4984 3338 Size: 1646 5921 Bme1580I G_kGCm'C Cuts at: 1790 2045 2061 2350 2548 2655 2990 3633 3687 Size: 255 16 289 198 107 335 643 54 Cuts at: 3687 3775 3882 4211 4479 4776 4842 4855 5628 Size: 88 107 329 268 297 66 13 773 Cuts at: 5628 6784 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 773 643 335 329 297 289 268 255 198 107 107 88 66 54 16 13 BmgBI CAC'GTC Cuts at: 1318 4075 4204 4447 1318 Size: 2757 129 243 4438 Fragments arranged by size: 4438 2757 243 129 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2608 5298 6256 Size: 416 53 83 399 24 1191 2690 958 Cuts at: 6256 442 Size: 1753 Fragments arranged by size: 2690 1753 1191 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7567 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2249 2717 2884 3032 3134 3170 4415 4421 Size: 1010 468 167 148 102 36 1245 6 Cuts at: 4421 6227 1239 Size: 1806 2579 Fragments arranged by size: 2579 1806 1245 1010 468 167 148 102 36 6 Bpu10I CC'TnA_GC Cuts at: 3542 4166 3542 Size: 624 6943 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2657 2720 3383 5692 6557 6855 2657 Size: 63 663 2309 865 298 3369 Fragments arranged by size: 3369 2309 865 663 298 63 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4654 4714 6230 882 Size: 617 3155 60 1516 2219 Fragments arranged by size: 3155 2219 1516 617 60 BsaAI yAC'GTr Cuts at: 493 1204 2230 2587 2985 5358 493 Size: 711 1026 357 398 2373 2702 Fragments arranged by size: 2702 2373 1026 711 398 357 BsaBI GATnn'nnATC Cuts at: 3299 7561 3299 Size: 4262 3305 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2211 2241 2490 2816 Size: 53 83 186 936 678 30 249 326 Cuts at: 2816 2925 3953 3968 4002 4068 4226 4487 5374 Size: 109 1028 15 34 66 158 261 887 Cuts at: 5374 5756 6391 275 Size: 382 635 1451 Fragments arranged by size: 1451 1028 936 887 678 635 382 326 261 249 186 158 109 83 66 53 34 30 15 BsaWI w'CCGG_w Cuts at: 4759 6000 6398 6672 6819 7230 4759 Size: 1241 398 274 147 411 5096 Fragments arranged by size: 5096 1241 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2414 2444 3610 3640 4256 Size: 30 165 30 1011 30 1166 30 616 Cuts at: 4256 4286 1178 Size: 30 4459 Fragments arranged by size: 4459 1166 1011 616 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2024 2027 2030 2117 2321 2378 3224 3227 Size: 838 3 3 87 204 57 846 3 Cuts at: 3227 3266 3732 3737 3857 4118 1186 Size: 39 466 5 120 261 4635 Fragments arranged by size: 4635 846 838 466 261 204 120 87 57 39 5 3 3 3 BseYI C'CCAG_C Cuts at: 2195 2219 3021 3524 3686 3739 4048 4370 4504 Size: 24 802 503 162 53 309 322 134 Cuts at: 4504 4600 4742 4778 6770 7266 2195 Size: 96 142 36 1992 496 2496 Fragments arranged by size: 2496 1992 802 503 496 322 309 162 142 134 96 53 36 24 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2235 2627 3302 4805 1163 Size: 573 499 392 675 1503 3925 Fragments arranged by size: 3925 1503 675 573 499 392 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2128 2203 4095 4150 4192 4320 Size: 275 186 127 75 1892 55 42 128 Cuts at: 4320 4670 4720 5017 5778 6806 1540 Size: 350 50 297 761 1028 2301 Fragments arranged by size: 2301 1892 1028 761 350 297 275 186 128 127 75 55 50 42 BsiHKAI G_wGCw'C Cuts at: 2548 2736 5628 5713 6784 2548 Size: 188 2892 85 1071 3331 Fragments arranged by size: 3331 2892 1071 188 85 BsmI GAATG_Cn' Cuts at: 3023 5078 5171 3023 Size: 2055 93 5419 Fragments arranged by size: 5419 2055 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2837 2837 Size: 7567 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2045 2061 2350 2458 2548 2655 2736 Size: 399 255 16 289 108 90 107 81 Cuts at: 2736 2990 2997 3633 3687 3775 3882 3936 4211 Size: 254 7 636 54 88 107 54 275 Cuts at: 4211 4479 4530 4769 4776 4842 4855 4886 5628 Size: 268 51 239 7 66 13 31 742 Cuts at: 5628 5713 6784 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 636 399 289 275 268 255 254 239 108 107 107 90 88 85 81 66 54 54 51 31 16 13 7 7 BspEI T'CCGG_A Cuts at: 4759 7230 4759 Size: 2471 5096 BspHI T'CATG_A Cuts at: 5456 7186 5456 Size: 1730 5837 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2416 2555 2599 3330 4922 844 Size: 1572 139 44 731 1592 3489 Fragments arranged by size: 3489 1592 1572 731 139 44 BsrBI CCG'CTC Cuts at: 1394 1823 3709 3986 5454 1394 Size: 429 1886 277 1468 3507 Fragments arranged by size: 3507 1886 1468 429 277 BsrDI GCAATG_nn' Cuts at: 65 1248 6062 6236 65 Size: 1183 4814 174 1396 Fragments arranged by size: 4814 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4145 4315 4479 4611 4669 6211 6398 Size: 110 2335 170 164 132 58 1542 187 Cuts at: 6398 1700 Size: 2869 Fragments arranged by size: 2869 2335 1542 187 170 164 132 110 58 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7567 BssHII G'CGCG_C Cuts at: 1925 4085 4087 1925 Size: 2160 2 5405 Fragments arranged by size: 5405 2160 2 BssSI C'ACGA_G Cuts at: 2291 4241 4551 5627 6639 2291 Size: 1950 310 1076 1012 3219 Fragments arranged by size: 3219 1950 1076 1012 310 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7567 BstBI TT'CG_AA Cuts at: 6371 6371 Size: 7567 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7567 BstXI CCAn_nnnn'nTGG Cuts at: 4048 4384 4544 4798 4048 Size: 336 160 254 6817 Fragments arranged by size: 6817 336 254 160 BstYI r'GATC_y Cuts at: 1972 2280 3494 4897 5264 5649 5666 7118 7204 Size: 308 1214 1403 367 385 17 1452 86 Cuts at: 7204 7216 7562 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1403 1214 385 367 346 308 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2483 2483 Size: 7567 BtgI C'CryG_G Cuts at: 513 1065 2004 2052 2070 2256 2934 3037 3564 Size: 552 939 48 18 186 678 103 527 Cuts at: 3564 3622 3956 4157 4186 4471 4530 4537 4855 Size: 58 334 201 29 285 59 7 318 Cuts at: 4855 513 Size: 3225 Fragments arranged by size: 3225 939 678 552 527 334 318 285 201 186 103 59 58 48 29 18 7 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3793 487 Size: 683 56 2567 4261 Fragments arranged by size: 4261 2567 683 56 BtsI GCAGTG_nn' Cuts at: 2418 2860 3592 3604 3920 4283 4793 5083 5877 Size: 442 732 12 316 363 510 290 794 Cuts at: 5877 5897 2418 Size: 20 4088 Fragments arranged by size: 4088 794 732 510 442 363 316 290 20 12 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7567 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7532 DraI TTT'AAA Cuts at: 4871 4871 Size: 7567 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6574 809 Size: 507 5258 1802 Fragments arranged by size: 5258 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2028 2200 2931 Size: 54 1425 50 275 18 198 172 731 Cuts at: 2931 3044 4077 4092 4147 4189 4233 4317 4667 Size: 113 1033 15 55 42 44 84 350 Cuts at: 4667 4717 5014 5903 7532 8 Size: 50 297 889 1629 43 Fragments arranged by size: 1629 1425 1033 889 731 350 297 275 198 172 113 84 55 54 50 50 44 43 42 18 15 EagI C'GGCC_G Cuts at: 1537 1812 2200 4092 4147 4189 4317 4667 4717 Size: 275 388 1892 55 42 128 350 50 Cuts at: 4717 5014 1537 Size: 297 4090 Fragments arranged by size: 4090 1892 388 350 297 275 128 55 50 42 EarI CTCTTCn'nnn_ Cuts at: 1353 2846 4136 5497 1353 Size: 1493 1290 1361 3423 Fragments arranged by size: 3423 1493 1361 1290 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4498 6154 6528 6674 387 Size: 1064 138 2909 1656 374 146 1280 Fragments arranged by size: 2909 1656 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2249 2717 2884 3032 3134 3170 3752 Size: 393 617 468 167 148 102 36 582 Cuts at: 3752 4415 4421 5630 6227 7014 7282 1239 Size: 663 6 1209 597 787 268 1524 Fragments arranged by size: 1524 1209 787 663 617 597 582 468 393 268 167 148 102 36 6 EcoNI CCTnn'n_nnAGG Cuts at: 3011 3701 3011 Size: 690 6877 EcoO109I rG'GnC_Cy Cuts at: 2057 2347 2404 2668 2799 2905 3025 3630 3683 Size: 290 57 264 131 106 120 605 53 Cuts at: 3683 4162 4208 4417 4591 4773 4851 2057 Size: 479 46 209 174 182 78 4773 Fragments arranged by size: 4773 605 479 290 264 209 182 174 131 120 106 78 57 53 46 EcoRI G'AATT_C Cuts at: 4876 5254 4876 Size: 378 7189 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3394 3394 Size: 7567 FspI TGC'GCA Cuts at: 6073 6073 Size: 7567 HaeII r_GCGC'y Cuts at: 1678 2244 2493 2793 2819 3244 3956 4005 4015 Size: 566 249 300 26 425 712 49 10 Cuts at: 4015 4028 4071 4117 4229 4505 6714 1678 Size: 13 43 46 112 276 2209 2531 Fragments arranged by size: 2531 2209 712 566 425 300 276 249 112 49 46 43 26 13 10 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2110 2142 2660 2692 4256 Size: 32 135 32 538 32 518 32 1564 Cuts at: 4256 4288 6208 6240 6282 6314 1373 Size: 32 1920 32 42 32 2626 Fragments arranged by size: 2626 1920 1564 538 518 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2596 4911 5157 669 Size: 988 281 658 2315 246 3079 Fragments arranged by size: 3079 2315 988 658 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7567 HpaI GTT'AAC Cuts at: 5157 5157 Size: 7567 KasI G'GCGC_C Cuts at: 2240 2489 2815 3952 4001 4067 4225 2240 Size: 249 326 1137 49 66 158 5582 Fragments arranged by size: 5582 1137 326 249 158 66 49 KpnI G_GTAC'C Cuts at: 4892 4892 Size: 7567 MfeI C'AATT_G Cuts at: 5166 5166 Size: 7567 MluI A'CGCG_T Cuts at: 4277 7443 4277 Size: 3166 4401 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3497 4287 6681 6865 1305 Size: 2192 790 2394 184 2007 Fragments arranged by size: 2394 2192 2007 790 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2030 10 Size: 54 1425 343 198 5547 Fragments arranged by size: 5547 1425 343 198 54 MslI CAynn'nnrTG Cuts at: 518 1166 2318 2640 2783 4241 4808 5525 5884 Size: 648 1152 322 143 1458 567 717 359 Cuts at: 5884 6043 518 Size: 159 2042 Fragments arranged by size: 2042 1458 1152 717 648 567 359 322 159 143 MspA1I CmG'CkG Cuts at: 1606 2054 2072 2199 2249 2258 2269 2420 3259 Size: 448 18 127 50 9 11 151 839 Cuts at: 3259 3337 3624 4111 4159 4473 4513 4532 4983 Size: 78 287 487 48 314 40 19 451 Cuts at: 4983 5660 6808 7053 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 839 677 487 451 448 314 287 245 151 127 78 50 48 40 19 18 11 9 NaeI GCC'GGC Cuts at: 4147 4613 4671 4147 Size: 466 58 7043 Fragments arranged by size: 7043 466 58 NarI GG'CG_CC Cuts at: 2241 2490 2816 3953 4002 4068 4226 2241 Size: 249 326 1137 49 66 158 5582 Fragments arranged by size: 5582 1137 326 249 158 66 49 NcoI C'CATG_G Cuts at: 513 1065 2004 4537 513 Size: 552 939 2533 3543 Fragments arranged by size: 3543 2533 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7567 NgoMIV G'CCGG_C Cuts at: 4145 4611 4669 4145 Size: 466 58 7043 Fragments arranged by size: 7043 466 58 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7567 NotI GC'GGCC_GC Cuts at: 2200 5014 2200 Size: 2814 4753 NspI r_CATG'y Cuts at: 1132 1229 3166 4461 4925 6470 1132 Size: 97 1937 1295 464 1545 2229 Fragments arranged by size: 2229 1937 1545 1295 464 97 PasI CC'CwG_GG Cuts at: 1348 4769 1348 Size: 3421 4146 PciI A'CATG_T Cuts at: 3162 6466 3162 Size: 3304 4263 PflMI CCAn_nnn'nTGG Cuts at: 1108 2037 4496 1108 Size: 929 2459 4179 Fragments arranged by size: 4179 2459 929 PfoI T'CCnGG_A Cuts at: 2694 3009 3246 2694 Size: 315 237 7015 Fragments arranged by size: 7015 315 237 PmeI GTTT'AAAC Cuts at: 4871 4871 Size: 7567 PmlI CAC'GTG Cuts at: 2587 2985 2587 Size: 398 7169 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5636 5668 7149 7181 5636 Size: 32 1481 32 6022 Fragments arranged by size: 6022 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2404 2668 2799 2905 3025 4417 2404 Size: 264 131 106 120 1392 5554 Fragments arranged by size: 5554 1392 264 131 120 106 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7567 PsiI TTA'TAA Cuts at: 5137 5137 Size: 7567 PspOMI G'GGCC_C Cuts at: 2057 2346 3629 3683 4207 4772 4851 2057 Size: 289 1283 54 524 565 79 4773 Fragments arranged by size: 4773 1283 565 524 289 79 54 PstI C_TGCA'G Cuts at: 830 2301 2463 3135 3702 4919 830 Size: 1471 162 672 567 1217 3478 Fragments arranged by size: 3478 1471 1217 672 567 162 PvuI CG_AT'CG Cuts at: 2001 2128 2001 Size: 127 7440 PvuII CAG'CTG Cuts at: 2269 3259 3337 2269 Size: 990 78 6499 Fragments arranged by size: 6499 990 78 SacII CC_GC'GG Cuts at: 2055 2073 2259 3625 4160 4474 4533 2055 Size: 18 186 1366 535 314 59 5089 Fragments arranged by size: 5089 1366 535 314 186 59 18 SalI G'TCGA_C Cuts at: 1936 4909 1936 Size: 2973 4594 SanDI GG'GwC_CC Cuts at: 3025 3025 Size: 7567 SbfI CC_TGCA'GG Cuts at: 4919 4919 Size: 7567 ScaI AGT'ACT Cuts at: 5815 7527 5815 Size: 1712 5855 SfcI C'TryA_G Cuts at: 826 1045 2297 2459 3131 3698 4915 6050 6731 Size: 219 1252 162 672 567 1217 1135 681 Cuts at: 6731 6922 826 Size: 191 1471 Fragments arranged by size: 1471 1252 1217 1135 681 672 567 219 191 162 SfiI GGCCn_nnn'nGGCC Cuts at: 4316 4316 Size: 7567 SfoI GGC'GCC Cuts at: 2242 2491 2817 3954 4003 4069 4227 2242 Size: 249 326 1137 49 66 158 5582 Fragments arranged by size: 5582 1137 326 249 158 66 49 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7567 SgrAI Cr'CCGG_yG Cuts at: 1700 4479 1700 Size: 2779 4788 SmaI CCC'GGG Cuts at: 1872 2537 2618 3356 3942 4894 1872 Size: 665 81 738 586 952 4545 Fragments arranged by size: 4545 952 738 665 586 81 SmlI C'TyrA_G Cuts at: 820 1017 1943 2672 2735 3398 5671 6572 6834 Size: 197 926 729 63 663 2273 901 262 Cuts at: 6834 820 Size: 1553 Fragments arranged by size: 2273 1553 926 901 729 663 262 197 63 SnaBI TAC'GTA Cuts at: 493 5358 493 Size: 4865 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7567 SphI G_CATG'C Cuts at: 1132 4461 4925 1132 Size: 3329 464 3774 Fragments arranged by size: 3774 3329 464 SspI AAT'ATT Cuts at: 5 52 5491 5 Size: 47 5439 2081 Fragments arranged by size: 5439 2081 47 StuI AGG'CCT Cuts at: 1517 3467 1517 Size: 1950 5617 StyI C'CwwG_G Cuts at: 513 1065 2004 2031 2928 3183 4537 5006 513 Size: 552 939 27 897 255 1354 469 3074 Fragments arranged by size: 3074 1354 939 897 552 469 255 27 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3820 4372 4457 5594 5611 5764 5949 Size: 1255 2240 552 85 1137 17 153 185 Cuts at: 5949 6394 325 Size: 445 1498 Fragments arranged by size: 2240 1498 1255 1137 552 445 185 153 85 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5813 7525 96 Size: 274 80 33 51 1443 3836 1712 138 Fragments arranged by size: 3836 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2414 2446 3610 3642 7527 7559 2414 Size: 32 1164 32 3885 32 2422 Fragments arranged by size: 3885 2422 1164 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3901 3970 1316 Size: 2585 69 4913 Fragments arranged by size: 4913 2585 69 XbaI T'CTAG_A Cuts at: 4903 4903 Size: 7567 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2203 2227 4384 2203 Size: 24 2157 5386 Fragments arranged by size: 5386 2157 24 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7567 XmaI C'CCGG_G Cuts at: 1870 2535 2616 3354 3940 4892 1870 Size: 665 81 738 586 952 4545 Fragments arranged by size: 4545 952 738 665 586 81 XmnI GAAnn'nnTTC Cuts at: 5696 5696 Size: 7567 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5375 276 Size: 53 83 186 936 3841 2468 Fragments arranged by size: 3841 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I DraIII EcoICRI EcoRV FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SacI SapI SexAI SrfI SwaI