Restriction map for
pFN21AE3768
(Linear) (Six-base) MAPSORT of: KIEE3768.seq Check: 8923 from: 1 to: 2862
KIEE3768.orf
With 182 enzymes: SgfI *
April 23, 2019 13:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 411 594 1325 2862
Size: 411 183 731 1537
Fragments arranged by size:
1537 731 411 183
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1747 2862
Size: 1747 1115
AfeI AGC'GCT
Cuts at: 0 786 1237 2862
Size: 786 451 1625
Fragments arranged by size:
1625 786 451
AflIII A'CryG_T
Cuts at: 0 1157 2272 2862
Size: 1157 1115 590
Fragments arranged by size:
1157 1115 590
AhdI GACnn_n'nnGTC
Cuts at: 0 669 2862
Size: 669 2193
AleI CACnn'nnGTG
Cuts at: 0 2236 2862
Size: 2236 626
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1405 1888 2862
Size: 1405 483 974
Fragments arranged by size:
1405 974 483
AlwNI CAG_nnn'CTG
Cuts at: 0 1275 2589 2862
Size: 1275 1314 273
Fragments arranged by size:
1314 1275 273
ApaI G_GGCC'C
Cuts at: 0 56 345 1628 1682 2206 2771 2850 2862
Size: 56 289 1283 54 524 565 79 12
Fragments arranged by size:
1283 565 524 289 79 56 54 12
ApaLI G'TGCA_C
Cuts at: 0 539 2862
Size: 539 2323
AvaI C'yCGr_G
Cuts at: 0 530 611 985 1349 1935 2518 2652 2862
Size: 530 81 374 364 586 583 134 210
Fragments arranged by size:
586 583 530 374 364 210 134 81
BamHI G'GATC_C
Cuts at: 0 1489 2862
Size: 1489 1373
BanI G'GyrC_C
Cuts at: 0 235 484 570 810 1947 1996 2062 2220
Size: 235 249 86 240 1137 49 66 158
Cuts at: 2220 2471 2862
Size: 251 391
Fragments arranged by size:
1137 391 251 249 240 235 158 86
66 49
BanII G_rGCy'C
Cuts at: 0 56 345 453 992 1628 1682 1931 2206
Size: 56 289 108 539 636 54 249 275
Cuts at: 2206 2525 2764 2771 2850 2862
Size: 319 239 7 79 12
Fragments arranged by size:
636 539 319 289 275 249 239 108
79 56 54 12 7
BbeI G_GCGC'C
Cuts at: 0 239 488 814 1951 2000 2066 2224 2862
Size: 239 249 326 1137 49 66 158 638
Fragments arranged by size:
1137 638 326 249 239 158 66 49
BbsI GAAGACnn'nnnn_
Cuts at: 0 201 1139 2201 2862
Size: 201 938 1062 661
Fragments arranged by size:
1062 938 661 201
BbvCI CC'TCA_GC
Cuts at: 0 1537 2161 2862
Size: 1537 624 701
Fragments arranged by size:
1537 701 624
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 976 1010 2862
Size: 976 34 1852
Fragments arranged by size:
1852 976 34
BglI GCCn_nnn'nGGC
Cuts at: 0 203 808 2071 2311 2862
Size: 203 605 1263 240 551
Fragments arranged by size:
1263 605 551 240 203
BlpI GC'TnA_GC
Cuts at: 0 1333 2862
Size: 1333 1529
Bme1580I G_kGCm'C
Cuts at: 0 40 56 345 543 650 985 1628 1682
Size: 40 16 289 198 107 335 643 54
Cuts at: 1682 1770 1877 2206 2474 2771 2837 2850 2862
Size: 88 107 329 268 297 66 13 12
Fragments arranged by size:
643 335 329 297 289 268 198 107
107 88 66 54 40 16 13 12
BmgBI CAC'GTC
Cuts at: 0 2070 2199 2442 2862
Size: 2070 129 243 420
Fragments arranged by size:
2070 420 243 129
BmrI ACTGGGnnnn_n'
Cuts at: 0 603 2862
Size: 603 2259
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 712 879 1027 1129 1165 2410 2416
Size: 244 468 167 148 102 36 1245 6
Cuts at: 2416 2862
Size: 446
Fragments arranged by size:
1245 468 446 244 167 148 102 36
6
Bpu10I CC'TnA_GC
Cuts at: 0 1537 2161 2862
Size: 1537 624 701
Fragments arranged by size:
1537 701 624
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 652 715 1378 2862
Size: 652 63 663 1484
Fragments arranged by size:
1484 663 652 63
BsaI GGTCTCn'nnnn_
Cuts at: 0 2649 2709 2862
Size: 2649 60 153
Fragments arranged by size:
2649 153 60
BsaAI yAC'GTr
Cuts at: 0 225 582 980 2862
Size: 225 357 398 1882
Fragments arranged by size:
1882 398 357 225
BsaBI GATnn'nnATC
Cuts at: 0 1294 2862
Size: 1294 1568
BsaHI Gr'CG_yC
Cuts at: 0 206 236 485 811 920 1948 1963 1997
Size: 206 30 249 326 109 1028 15 34
Cuts at: 1997 2063 2221 2482 2862
Size: 66 158 261 380
Fragments arranged by size:
1028 380 326 261 249 206 158 109
66 34 30 15
BsaWI w'CCGG_w
Cuts at: 0 2754 2862
Size: 2754 108
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 409 439 1605 1635 2251 2281 2862
Size: 409 30 1166 30 616 30 581
Fragments arranged by size:
1166 616 581 409 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 19 22 25 112 316 373 1219 1222
Size: 19 3 3 87 204 57 846 3
Cuts at: 1222 1261 1727 1732 1852 2113 2862
Size: 39 466 5 120 261 749
Fragments arranged by size:
846 749 466 261 204 120 87 57
39 19 5 3 3 3
BseYI C'CCAG_C
Cuts at: 0 190 214 1016 1519 1681 1734 2043 2365
Size: 190 24 802 503 162 53 309 322
Cuts at: 2365 2499 2595 2737 2773 2862
Size: 134 96 142 36 89
Fragments arranged by size:
802 503 322 309 190 162 142 134
96 89 53 36 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 230 622 1297 2800 2862
Size: 230 392 675 1503 62
Fragments arranged by size:
1503 675 392 230 62
BsiEI CG_ry'CG
Cuts at: 0 123 198 2090 2145 2187 2315 2665 2715
Size: 123 75 1892 55 42 128 350 50
Cuts at: 2715 2862
Size: 147
Fragments arranged by size:
1892 350 147 128 123 75 55 50
42
BsiHKAI G_wGCw'C
Cuts at: 0 543 731 2862
Size: 543 188 2131
Fragments arranged by size:
2131 543 188
BsmI GAATG_Cn'
Cuts at: 0 1018 2862
Size: 1018 1844
BsmBI CGTCTCn'nnnn_
Cuts at: 0 832 2862
Size: 832 2030
Bsp1286I G_dGCh'C
Cuts at: 0 40 56 345 453 543 650 731 985
Size: 40 16 289 108 90 107 81 254
Cuts at: 985 992 1628 1682 1770 1877 1931 2206 2474
Size: 7 636 54 88 107 54 275 268
Cuts at: 2474 2525 2764 2771 2837 2850 2862
Size: 51 239 7 66 13 12
Fragments arranged by size:
636 289 275 268 254 239 108 107
107 90 88 81 66 54 54 51
40 16 13 12 7 7
BspEI T'CCGG_A
Cuts at: 0 2754 2862
Size: 2754 108
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 411 550 594 1325 2862
Size: 411 139 44 731 1537
Fragments arranged by size:
1537 731 411 139 44
BsrBI CCG'CTC
Cuts at: 0 1704 1981 2862
Size: 1704 277 881
Fragments arranged by size:
1704 881 277
BsrFI r'CCGG_y
Cuts at: 0 2140 2310 2474 2606 2664 2862
Size: 2140 170 164 132 58 198
Fragments arranged by size:
2140 198 170 164 132 58
BssHII G'CGCG_C
Cuts at: 0 2080 2082 2862
Size: 2080 2 780
Fragments arranged by size:
2080 780 2
BssSI C'ACGA_G
Cuts at: 0 286 2236 2546 2862
Size: 286 1950 310 316
Fragments arranged by size:
1950 316 310 286
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2043 2379 2539 2793 2862
Size: 2043 336 160 254 69
Fragments arranged by size:
2043 336 254 160 69
BstYI r'GATC_y
Cuts at: 0 275 1489 2862
Size: 275 1214 1373
Fragments arranged by size:
1373 1214 275
Bsu36I CC'TnA_GG
Cuts at: 0 478 2862
Size: 478 2384
BtgI C'CryG_G
Cuts at: 0 47 65 251 929 1032 1559 1617 1951
Size: 47 18 186 678 103 527 58 334
Cuts at: 1951 2152 2181 2466 2525 2532 2850 2862
Size: 201 29 285 59 7 318 12
Fragments arranged by size:
678 527 334 318 285 201 186 103
59 58 47 29 18 12 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1788 2862
Size: 1788 1074
BtsI GCAGTG_nn'
Cuts at: 0 413 855 1587 1599 1915 2278 2788 2862
Size: 413 442 732 12 316 363 510 74
Fragments arranged by size:
732 510 442 413 363 316 74 12
EaeI y'GGCC_r
Cuts at: 0 23 195 926 1039 2072 2087 2142 2184
Size: 23 172 731 113 1033 15 55 42
Cuts at: 2184 2228 2312 2662 2712 2862
Size: 44 84 350 50 150
Fragments arranged by size:
1033 731 350 172 150 113 84 55
50 44 42 23 15
EagI C'GGCC_G
Cuts at: 0 195 2087 2142 2184 2312 2662 2712 2862
Size: 195 1892 55 42 128 350 50 150
Fragments arranged by size:
1892 350 195 150 128 55 50 42
EarI CTCTTCn'nnn_
Cuts at: 0 841 2131 2862
Size: 841 1290 731
Fragments arranged by size:
1290 841 731
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2493 2862
Size: 2493 369
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 712 879 1027 1129 1165 1747 2410
Size: 244 468 167 148 102 36 582 663
Cuts at: 2410 2416 2862
Size: 6 446
Fragments arranged by size:
663 582 468 446 244 167 148 102
36 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1006 1696 2862
Size: 1006 690 1166
Fragments arranged by size:
1166 1006 690
EcoO109I rG'GnC_Cy
Cuts at: 0 52 342 399 663 794 900 1020 1625
Size: 52 290 57 264 131 106 120 605
Cuts at: 1625 1678 2157 2203 2412 2586 2768 2846 2862
Size: 53 479 46 209 174 182 78 16
Fragments arranged by size:
605 479 290 264 209 182 174 131
120 106 78 57 53 52 46 16
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1389 2862
Size: 1389 1473
HaeII r_GCGC'y
Cuts at: 0 239 488 788 814 1239 1951 2000 2010
Size: 239 249 300 26 425 712 49 10
Cuts at: 2010 2023 2066 2112 2224 2500 2862
Size: 13 43 46 112 276 362
Fragments arranged by size:
712 425 362 300 276 249 239 112
49 46 43 26 13 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 105 137 655 687 2251 2283 2862
Size: 105 32 518 32 1564 32 579
Fragments arranged by size:
1564 579 518 105 32 32 32
HincII GTy'rAC
Cuts at: 0 591 2862
Size: 591 2271
KasI G'GCGC_C
Cuts at: 0 235 484 810 1947 1996 2062 2220 2862
Size: 235 249 326 1137 49 66 158 642
Fragments arranged by size:
1137 642 326 249 235 158 66 49
MluI A'CGCG_T
Cuts at: 0 2272 2862
Size: 2272 590
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1492 2282 2862
Size: 1492 790 580
Fragments arranged by size:
1492 790 580
MscI TGG'CCA
Cuts at: 0 25 2862
Size: 25 2837
MslI CAynn'nnrTG
Cuts at: 0 313 635 778 2236 2803 2862
Size: 313 322 143 1458 567 59
Fragments arranged by size:
1458 567 322 313 143 59
MspA1I CmG'CkG
Cuts at: 0 49 67 194 244 253 264 415 1254
Size: 49 18 127 50 9 11 151 839
Cuts at: 1254 1332 1619 2106 2154 2468 2508 2527 2862
Size: 78 287 487 48 314 40 19 335
Fragments arranged by size:
839 487 335 314 287 151 127 78
50 49 48 40 19 18 11 9
NaeI GCC'GGC
Cuts at: 0 2142 2608 2666 2862
Size: 2142 466 58 196
Fragments arranged by size:
2142 466 196 58
NarI GG'CG_CC
Cuts at: 0 236 485 811 1948 1997 2063 2221 2862
Size: 236 249 326 1137 49 66 158 641
Fragments arranged by size:
1137 641 326 249 236 158 66 49
NcoI C'CATG_G
Cuts at: 0 2532 2862
Size: 2532 330
NgoMIV G'CCGG_C
Cuts at: 0 2140 2606 2664 2862
Size: 2140 466 58 198
Fragments arranged by size:
2140 466 198 58
NotI GC'GGCC_GC
Cuts at: 0 195 2862
Size: 195 2667
NspI r_CATG'y
Cuts at: 0 1161 2456 2862
Size: 1161 1295 406
Fragments arranged by size:
1295 1161 406
PasI CC'CwG_GG
Cuts at: 0 2764 2862
Size: 2764 98
PciI A'CATG_T
Cuts at: 0 1157 2862
Size: 1157 1705
PflMI CCAn_nnn'nTGG
Cuts at: 0 32 2491 2862
Size: 32 2459 371
Fragments arranged by size:
2459 371 32
PfoI T'CCnGG_A
Cuts at: 0 689 1004 1241 2862
Size: 689 315 237 1621
Fragments arranged by size:
1621 689 315 237
PmlI CAC'GTG
Cuts at: 0 582 980 2862
Size: 582 398 1882
Fragments arranged by size:
1882 582 398
PpuMI rG'GwC_Cy
Cuts at: 0 399 663 794 900 1020 2412 2862
Size: 399 264 131 106 120 1392 450
Fragments arranged by size:
1392 450 399 264 131 120 106
PspOMI G'GGCC_C
Cuts at: 0 52 341 1624 1678 2202 2767 2846 2862
Size: 52 289 1283 54 524 565 79 16
Fragments arranged by size:
1283 565 524 289 79 54 52 16
PstI C_TGCA'G
Cuts at: 0 296 458 1130 1697 2862
Size: 296 162 672 567 1165
Fragments arranged by size:
1165 672 567 296 162
PvuI CG_AT'CG
Cuts at: 0 123 2862
Size: 123 2739
PvuII CAG'CTG
Cuts at: 0 264 1254 1332 2862
Size: 264 990 78 1530
Fragments arranged by size:
1530 990 264 78
SacII CC_GC'GG
Cuts at: 0 50 68 254 1620 2155 2469 2528 2862
Size: 50 18 186 1366 535 314 59 334
Fragments arranged by size:
1366 535 334 314 186 59 50 18
SanDI GG'GwC_CC
Cuts at: 0 1020 2862
Size: 1020 1842
SfcI C'TryA_G
Cuts at: 0 292 454 1126 1693 2862
Size: 292 162 672 567 1169
Fragments arranged by size:
1169 672 567 292 162
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2311 2862
Size: 2311 551
SfoI GGC'GCC
Cuts at: 0 237 486 812 1949 1998 2064 2222 2862
Size: 237 249 326 1137 49 66 158 640
Fragments arranged by size:
1137 640 326 249 237 158 66 49
SgrAI Cr'CCGG_yG
Cuts at: 0 2474 2862
Size: 2474 388
SmaI CCC'GGG
Cuts at: 0 532 613 1351 1937 2862
Size: 532 81 738 586 925
Fragments arranged by size:
925 738 586 532 81
SmlI C'TyrA_G
Cuts at: 0 667 730 1393 2862
Size: 667 63 663 1469
Fragments arranged by size:
1469 667 663 63
SphI G_CATG'C
Cuts at: 0 2456 2862
Size: 2456 406
StuI AGG'CCT
Cuts at: 0 1462 2862
Size: 1462 1400
StyI C'CwwG_G
Cuts at: 0 26 923 1178 2532 2862
Size: 26 897 255 1354 330
Fragments arranged by size:
1354 897 330 255 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1815 2367 2452 2862
Size: 1815 552 85 410
Fragments arranged by size:
1815 552 410 85
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 409 441 1605 1637 2862
Size: 409 32 1164 32 1225
Fragments arranged by size:
1225 1164 409 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1896 1965 2862
Size: 1896 69 897
Fragments arranged by size:
1896 897 69
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 198 222 2379 2862
Size: 198 24 2157 483
Fragments arranged by size:
2157 483 198 24
XmaI C'CCGG_G
Cuts at: 0 530 611 1349 1935 2862
Size: 530 81 738 586 927
Fragments arranged by size:
927 738 586 530 81
Enzymes that do cut:
AarI AcuI AfeI AflIII AhdI AleI AlfI AlwNI
ApaI ApaLI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BglI BlpI Bme1580I BmgBI BmrI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspMI BsrBI BsrFI BssHII BssSI BstXI BstYI
Bsu36I BtgI BtgZI BtsI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII KasI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NotI NspI PasI PciI PflMI PfoI PmlI
PpuMI PspOMI PstI PvuI PvuII SacII SanDI SfcI
SfiI SfoI SgrAI SmaI SmlI SphI StuI StyI
TaqII TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AloI ApoI
AscI AseI AsiSI AvrII BaeI BciVI BclI BfrBI
BglII BmtI BplI BsiWI BspHI BsrDI BsrGI BstAPI
BstBI BstEII BstZ17I ClaI CspCI DraI DraIII DrdI
EcoICRI EcoRI EcoRV FseI FspI FspAI HindIII HpaI
KpnI MfeI NdeI NheI NruI NsiI PacI PmeI
PpiI PshAI PsiI PspXI PsrI RsrII SacI SalI
SapI SbfI ScaI SexAI SgfI SnaBI SpeI SrfI
SspI SwaI TatI XbaI XhoI XmnI ZraI