################################## # NO CUT ENZYMES ( 104 enzymes) # ################################## AatII Acc65I AclI AflIII AgeI Alw44I AlwI AocI Aor51HI ApaLI AscI AseI AsnI Asp718I AspI AvrII BamHI BbsI BciVI BclI BfaI BglII BlnI BpmI BpuAI BsaBI BsaI BsiWI BsmBI BsmI Bsp1407I BspDI BspHI BspLU11I BsrDI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I ClaI DraI DraIII DrdI Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI HindIII HpaI HphI KpnI MaeI MaeIII MamI MfeI MflI MluI MslI MunI NcoI NdeI NheI NruI NsiI NspV PacI PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I RcaI SbfI ScaI SexAI SfuI SnaBI SpeI Sse8387I SspBI SspI StuI StyI SwaI Tsp45I TspRI Tth111I Van91I XbaI XhoII ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccIII 1098 TCCGGA T^CCGGA AcsI 352 AAATTC [GA]^AATT[CT] AflII 301 CTTAAG C^TTAAG AhdI 1351 GACTCGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApaI 1040 GGGCCC GGGCC^C ApoI 352 AAATTC [GA]^AATT[CT] Asp700I 348 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 1351 GACTCGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 548 TGCGCA TGC^GCA BalI 65 TGGCCA TGG^CCA BbrPI 498 CACGTG CAC^GTG BcgI 1267 GCACCTCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 301 CTTAAG C^TTAAG BlpI 1104 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1104 GCTCAGC GC^T[ACGT]AGC BsaAI 498 CACGTG [CT]AC^GT[GA] BsaWI 1098 TCCGGA [AT]^CCGG[AT] BseAI 1098 TCCGGA T^CCGGA BsmAI 1419 GTCTC GTCTC(1/5) BspEI 1098 TCCGGA T^CCGGA BsrI 522 CCAGT ACTGG(1/-1) CelII 1104 GCTCAGC GC^T[ACGT]AGC Eam1105I 1351 GACTCGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 879 CTCTTC CTCTTC(1/4) FspI 548 TGCGCA TGC^GCA Ksp632I 879 CTCTTC CTCTTC(1/4) MluNI 65 TGGCCA TGG^CCA MroI 1098 TCCGGA T^CCGGA MscI 65 TGGCCA TGG^CCA MseI 302 TTAA T^TAA PaeR7I 820 CTCGAG C^TCGAG PmaCI 498 CACGTG CAC^GTG PmlI 498 CACGTG CAC^GTG PstI 409 CTGCAG CTGCA^G PvuI 384 CGATCG CGAT^CG SapI 878 GCTCTTC GCTCTTC(1/4) SfaNI 718 GATGC GCATC(5/9) SfcI 409 CTGCAG C^T[GA][CT]AG SfiI 924 GGCCTCCGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SgrAI 1226 CGCCGGCG C[GA]^CCGG[CT]G SphI 183 GCATGC GCATG^C TfiI 152 GATTC G^A[AT]TC Tru9I 302 TTAA T^TAA Tsp509I 353 AATT ^AATT XcmI 522 CCAGTTCGCGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 820 CTCGAG C^TCGAG XmnI 348 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccI 3 GTCGAC GT^[AC][GT]AC 34 GTCGAC AlwNI 252 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG 1201 CAGGGCCTG AspHI 1102 GAGCTC G[AT]GC[AT]^C 1392 GAGCTC BbvCI 771 CCTCAGC CCTCAGC(-5/-2) 1404 CCTCAGC BsgI 403 CTGCAC GTGCAG(16/14) 760 CTGCAC BsiHKAI 1102 GAGCTC G[AT]GC[AT]^C 1392 GAGCTC BssSI 541 CACGAG CACGAG(-5/-1) 1068 CTCGTG BstAPI 251 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 396 GCACCTCCTGC DpnII 385 GATC ^GATC 444 GATC HgaI 111 GACGC GACGC(5/10) 1330 GACGC HincII 3 GTCGAC GT[CT]^[GA]AC 34 GTCGAC HindII 3 GTCGAC GT[CT]^[GA]AC 34 GTCGAC MaeII 499 ACGT A^CGT 850 ACGT MboI 385 GATC ^GATC 444 GATC MboII 880 TCTTC GAAGA(8/7) 1454 TCTTC NdeII 385 GATC ^GATC 444 GATC NotI 976 GCGGCCGC GC^GGCCGC 988 GCGGCCGC PpuMI 692 GGGACCT [GA]G^G[AT]CC[CT] 1022 AGGACCT SacI 1102 GAGCTC GAGCT^C 1392 GAGCTC SalI 3 GTCGAC G^TCGAC 34 GTCGAC Sau3AI 385 GATC ^GATC 444 GATC SmaI 234 CCCGGG CCC^GGG 810 CCCGGG SmlI 301 CTTAAG C^T[CT][GA]AG 820 CTCGAG XmaCI 234 CCCGGG C^CCGGG 810 CCCGGG XmaI 234 CCCGGG C^CCGGG 810 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 82 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AvaI AvaII BanI BanII BbeI BbvI BcnI BglI BmyI BsaHI BsaJI BseRI BsiEI BsiYI BslI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I CpoI DdeI DraII DsaI EaeI EagI EclXI Eco52I EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII ItaI KasI KspI MnlI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NgoMIV NlaIII NlaIV NspI PleI PvuII RsaI RsrII SacII Sau96I ScrFI SfoI TaqI TseI TthHB8I