################################## # NO CUT ENZYMES ( 99 enzymes) # ################################## AatII AccI AccII AclI AflII AgeI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI AviII AvrII BbeI BbvCI BcgI BcnI BfrI BglII BlnI BsaBI BsgI BsiEI BsiWI BsmBI BspDI BsrBI BsrFI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Cfr10I ClaI CpoI DraIII DrdI DsaI EagI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV FseI FspI HaeII KasI KspI MamI MfeI MluI MunI MvnI NaeI NarI NciI NcoI NgoMIV NotI NruI NspV PacI PaeR7I PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacII SalI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI SspI SwaI Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### Acc65I 2232 GGTACC G^GTACC AccIII 1967 TCCGGA T^CCGGA AcyI 304 GGCGTC G[GA]^CG[CT]C AflIII 2954 ACATGT A^C[GA][CT]GT AhdI 1317 GACTATAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 2087 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 2493 CCTCAGG CC^T[ACGT]AGG Asp718I 2232 GGTACC G^GTACC AspEI 1317 GACTATAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 478 GAGCTC G[AT]GC[AT]^C AspI 1099 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC BbrPI 749 CACGTG CAC^GTG BciVI 665 GGATAC GTATCC(6/5) BlpI 838 GCTGAGC GC^T[ACGT]AGC Bpu1102I 838 GCTGAGC GC^T[ACGT]AGC BsaAI 749 CACGTG [CT]AC^GT[GA] BsaHI 304 GGCGTC G[GA]^CG[CT]C BseAI 1967 TCCGGA T^CCGGA BsiHKAI 478 GAGCTC G[AT]GC[AT]^C Bsp1407I 559 TGTACA T^GTACA BspEI 1967 TCCGGA T^CCGGA BspHI 1503 TCATGA T^CATGA BspLU11I 2954 ACATGT A^CATGT BsrGI 559 TGTACA T^GTACA BssSI 2046 CTCGTG CACGAG(-5/-1) Bsu36I 2493 CCTCAGG CC^T[ACGT]AGG CelII 838 GCTGAGC GC^T[ACGT]AGC CfoI 1079 GCGC GCG^C DraI 2742 TTTAAA TTT^AAA DraII 815 AGGTCCC [GA]G^G[ACGT]CC[CT] Eam1105I 1317 GACTATAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 2493 CCTCAGG CC^T[ACGT]AGG EcoO109I 815 AGGTCCC [GA]G^G[ACGT]CC[CT] HgaI 305 GCGTC GACGC(5/10) HhaI 1079 GCGC GCG^C Hin1I 304 GGCGTC G[GA]^CG[CT]C HinP1I 1079 GCGC G^CGC KpnI 2232 GGTACC GGTAC^C MroI 1967 TCCGGA T^CCGGA NdeI 2152 CATATG CA^TATG NheI 1515 GCTAGC G^CTAGC PflFI 1099 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 749 CACGTG CAC^GTG PmlI 749 CACGTG CAC^GTG PpuMI 815 AGGTCCC [GA]G^G[AT]CC[CT] PstI 715 CTGCAG CTGCA^G RcaI 1503 TCATGA T^CATGA SacI 478 GAGCTC GAGCT^C SapI 2777 GCTCTTC GCTCTTC(1/4) SbfI 714 CCTGCAGG CCTGCA^GG SexAI 2027 ACCTGGT A^CC[AT]GGT SmlI 1129 CTTGAG C^T[CT][GA]AG Sse8387I 714 CCTGCAGG CCTGCA^GG SspBI 559 TGTACA T^GTACA StuI 2073 AGGCCT AGG^CCT Tth111I 1099 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AvaI 794 CTCGGG C^[CT]CG[GA]G 1680 CCCGAG BalI 24 TGGCCA TGG^CCA 2642 TGGCCA BanII 478 GAGCTC G[GA]GC[CT]^C 2465 GAGCCC BbsI 86 GTCTTC GAAGAC(2/6) 2032 GTCTTC BglI 26 GCCATGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1249 GCCCCTCTGGC BmyI 478 GAGCTC G[AGT]GC[ACT]^C 2465 GAGCCC BpuAI 86 GTCTTC GAAGAC(2/6) 2032 GTCTTC BsaI 2118 GGTCTC GGTCTC(1/5) 2895 GGTCTC BsaWI 1967 TCCGGA [AT]^CCGG[AT] 2319 ACCGGA BseRI 183 CTCCTC GAGGAG(10/8) 1742 GAGGAG BsoBI 794 CTCGGG C^[CT]CG[GA]G 1680 CCCGAG Bsp1286I 478 GAGCTC G[AGT]GC[ACT]^C 2465 GAGCCC BstXI 744 CCATCCACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 779 CCATCCCAGTGG EaeI 24 TGGCCA [CT]^GGCC[GA] 2642 TGGCCA EcoT14I 1166 CCTTGG C^C[AT][AT]GG 2763 CCAAGG EcoT22I 296 ATGCAT ATGCA^T 593 ATGCAT HpaI 1695 GTTAAC GTT^AAC 2566 GTTAAC MluNI 24 TGGCCA TGG^CCA 2642 TGGCCA MscI 24 TGGCCA TGG^CCA 2642 TGGCCA NsiI 296 ATGCAT ATGCA^T 593 ATGCAT StyI 1166 CCTTGG C^C[AT][AT]GG 2763 CCAAGG ######################################### # 3 OR MORE CUT ENZYMES ( 82 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI Asp700I AvaII BamHI BanI BbvI BclI BfaI BpmI BsaJI BsiYI BslI BsmAI BsmFI BsmI BspMI BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII EarI EcoRII FauI FbaI Fnu4HI FokI HaeIII HapII HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NspI PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI