(Circular) (Six-base) MAPSORT of: pFN21AE9436.seq Check: 2887 from: 1 to: 7756 pFN21AE9436 7756 bp With 182 enzymes: SgfI * March 28, 2012 11:59 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2164 3565 2164 Size: 1401 6355 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5566 278 Size: 53 83 186 936 4030 2468 Fragments arranged by size: 4030 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5099 1937 Size: 3162 4594 Acc65I G'GTAC_C Cuts at: 4237 5077 4237 Size: 840 6916 AclI AA'CG_TT Cuts at: 1563 5883 6256 1563 Size: 4320 373 3063 Fragments arranged by size: 4320 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2067 2693 2907 3407 5819 7203 7471 1632 Size: 435 626 214 500 2412 1384 268 1917 Fragments arranged by size: 2412 1917 1384 626 500 435 268 214 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7559 AflIII A'CryG_T Cuts at: 1203 4959 6655 7632 1203 Size: 3756 1696 977 1327 Fragments arranged by size: 3756 1696 1327 977 AgeI A'CCGG_T Cuts at: 6587 6587 Size: 7756 AhdI GACnn_n'nnGTC Cuts at: 3327 6485 3327 Size: 3158 4598 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7756 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7756 AlwNI CAG_nnn'CTG Cuts at: 4097 5499 7071 4097 Size: 1402 1572 4782 Fragments arranged by size: 4782 1572 1402 ApaLI G'TGCA_C Cuts at: 5813 6969 5813 Size: 1156 6600 ApoI r'AATT_y Cuts at: 1493 4214 4307 4714 4789 5065 5291 5443 7552 Size: 2721 93 407 75 276 226 152 2109 Cuts at: 7552 1493 Size: 1697 Fragments arranged by size: 2721 2109 1697 407 276 226 152 93 75 AseI AT'TA_AT Cuts at: 160 1032 6310 160 Size: 872 5278 1606 Fragments arranged by size: 5278 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7756 AvaI C'yCGr_G Cuts at: 1870 1943 2799 3685 5081 1870 Size: 73 856 886 1396 4545 Fragments arranged by size: 4545 1396 886 856 73 BamHI G'GATC_C Cuts at: 2725 3571 4295 5086 5453 2725 Size: 846 724 791 367 5028 Fragments arranged by size: 5028 846 791 724 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2438 2794 3011 4237 5077 Size: 325 217 627 651 356 217 1226 840 Cuts at: 5077 6532 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1226 840 651 627 356 325 217 217 BanII G_rGCy'C Cuts at: 1391 2487 4474 5075 1391 Size: 1096 1987 601 4072 Fragments arranged by size: 4072 1987 1096 601 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2084 4030 928 Size: 971 185 1946 4654 Fragments arranged by size: 4654 1946 971 185 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5947 5981 1903 Size: 34 4010 34 3678 Fragments arranged by size: 4010 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2664 5648 6858 2664 Size: 2984 1210 3562 Fragments arranged by size: 3562 2984 1210 BclI T'GATC_A Cuts at: 1548 3207 3360 3597 1548 Size: 1659 153 237 5707 Fragments arranged by size: 5707 1659 237 153 BfrBI ATG'CAT Cuts at: 2303 2600 2303 Size: 297 7459 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2037 3260 6367 Size: 107 122 71 631 605 365 1223 3107 Cuts at: 6367 136 Size: 1525 Fragments arranged by size: 3107 1525 1223 631 605 365 122 107 71 BglII A'GATC_T Cuts at: 7751 7751 Size: 7756 BlpI GC'TnA_GC Cuts at: 2844 5173 2844 Size: 2329 5427 Bme1580I G_kGCm'C Cuts at: 1790 5817 6973 1790 Size: 4027 1156 2573 Fragments arranged by size: 4027 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7756 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2783 4475 4538 Size: 416 53 83 399 24 1366 1692 63 Cuts at: 4538 5487 6445 442 Size: 949 958 1753 Fragments arranged by size: 1753 1692 1366 958 949 416 399 83 63 53 24 BmtI G_CTAG'C Cuts at: 1056 3524 1056 Size: 2468 5288 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3607 3607 Size: 7756 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2187 2469 2835 3203 3602 4744 6416 1239 Size: 948 282 366 368 399 1142 1672 2579 Fragments arranged by size: 2579 1672 1142 948 399 368 366 282 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3155 5881 6746 7044 3155 Size: 2726 865 298 3867 Fragments arranged by size: 3867 2726 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4129 4906 6419 882 Size: 617 2630 777 1513 2219 Fragments arranged by size: 2630 2219 1513 777 617 BsaAI yAC'GTr Cuts at: 493 1204 2756 5547 493 Size: 711 1552 2791 2702 Fragments arranged by size: 2791 2702 1552 711 BsaBI GATnn'nnATC Cuts at: 7750 7750 Size: 7756 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2310 5563 5945 6580 Size: 53 83 186 936 777 3253 382 635 Cuts at: 6580 275 Size: 1451 Fragments arranged by size: 3253 1451 936 777 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3972 4324 6189 6587 6861 7008 7419 3972 Size: 352 1865 398 274 147 411 4309 Fragments arranged by size: 4309 1865 411 398 352 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3004 3034 3430 3460 1178 Size: 30 165 30 1601 30 396 30 5474 Fragments arranged by size: 5474 1601 396 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2179 3762 1186 Size: 993 1583 5180 Fragments arranged by size: 5180 1583 993 BseYI C'CCAG_C Cuts at: 2916 4473 6959 7455 2916 Size: 1557 2486 496 3217 Fragments arranged by size: 3217 2486 1557 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 7183 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5206 5967 6995 1540 Size: 275 186 3205 761 1028 2301 Fragments arranged by size: 3205 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2487 5817 5902 6973 2487 Size: 3330 85 1071 3270 Fragments arranged by size: 3330 3270 1071 85 BsmI GAATG_Cn' Cuts at: 2602 3376 4151 5267 5360 2602 Size: 774 775 1116 93 4998 Fragments arranged by size: 4998 1116 775 774 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2487 4474 5075 5817 5902 6973 1391 Size: 399 697 1987 601 742 85 1071 2174 Fragments arranged by size: 2174 1987 1071 742 697 601 399 85 BspEI T'CCGG_A Cuts at: 3972 7419 3972 Size: 3447 4309 BspHI T'CATG_A Cuts at: 3508 5645 7375 3508 Size: 2137 1730 3889 Fragments arranged by size: 3889 2137 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2164 2727 3565 5111 844 Size: 1320 563 838 1546 3489 Fragments arranged by size: 3489 1546 1320 838 563 BsrBI CCG'CTC Cuts at: 1394 1823 5643 1394 Size: 429 3820 3507 Fragments arranged by size: 3820 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2359 4676 4993 6251 6425 65 Size: 1183 1111 2317 317 1258 174 1396 Fragments arranged by size: 2317 1396 1258 1183 1111 317 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6400 6587 1700 Size: 110 4590 187 2869 Fragments arranged by size: 4590 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2564 96 Size: 2468 5288 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7756 BssSI C'ACGA_G Cuts at: 4051 5816 6828 4051 Size: 1765 1012 4979 Fragments arranged by size: 4979 1765 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7756 BstBI TT'CG_AA Cuts at: 6560 6560 Size: 7756 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7756 BstXI CCAn_nnnn'nTGG Cuts at: 2756 2791 2756 Size: 35 7721 BstYI r'GATC_y Cuts at: 1972 2725 3571 3969 4295 5086 5453 5838 5855 Size: 753 846 398 326 791 367 385 17 Cuts at: 5855 7307 7393 7405 7751 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 846 791 753 398 385 367 346 326 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4499 4499 Size: 7756 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 6265 Fragments arranged by size: 6265 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3468 487 Size: 683 56 2242 4775 Fragments arranged by size: 4775 2242 683 56 BtsI GCAGTG_nn' Cuts at: 2030 2650 4829 5272 6066 6086 2030 Size: 620 2179 443 794 20 3700 Fragments arranged by size: 3700 2179 794 620 443 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7756 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7721 DraI TTT'AAA Cuts at: 4749 5060 4749 Size: 311 7445 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6763 809 Size: 507 5447 1802 Fragments arranged by size: 5447 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2029 4647 5203 Size: 54 1425 50 275 18 199 2618 556 Cuts at: 5203 6092 7721 8 Size: 889 1629 43 Fragments arranged by size: 2618 1629 1425 889 556 275 199 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5203 1537 Size: 275 3391 4090 Fragments arranged by size: 4090 3391 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2688 2986 3480 4789 5686 1353 Size: 1335 298 494 1309 897 3423 Fragments arranged by size: 3423 1335 1309 897 494 298 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6343 6717 6863 387 Size: 1064 138 4754 374 146 1280 Fragments arranged by size: 4754 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2485 2485 Size: 7756 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2067 2187 2469 2693 2835 2907 3203 Size: 393 435 120 282 224 142 72 296 Cuts at: 3203 3407 3602 4744 5819 6416 7203 7471 1239 Size: 204 195 1142 1075 597 787 268 1524 Fragments arranged by size: 1524 1142 1075 787 597 435 393 296 282 268 224 204 195 142 120 72 EcoO109I rG'GnC_Cy Cuts at: 2821 2821 Size: 7756 EcoRI G'AATT_C Cuts at: 5065 5443 5065 Size: 378 7378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2532 2532 Size: 7756 FspI TGC'GCA Cuts at: 6262 6262 Size: 7756 HaeII r_GCGC'y Cuts at: 1678 6903 1678 Size: 5225 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2364 2396 3192 3224 3313 Size: 32 135 32 792 32 796 32 89 Cuts at: 3313 3345 4666 4698 6397 6429 6471 6503 1373 Size: 32 1321 32 1699 32 42 32 2626 Fragments arranged by size: 2626 1699 1321 796 792 135 89 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3073 3112 3702 4573 5100 5346 Size: 988 281 1135 39 590 871 527 246 Cuts at: 5346 669 Size: 3079 Fragments arranged by size: 3079 1135 988 871 590 527 281 246 39 HindIII A'AGCT_T Cuts at: 748 2242 2705 3826 5032 748 Size: 1494 463 1121 1206 3472 Fragments arranged by size: 3472 1494 1206 1121 463 HpaI GTT'AAC Cuts at: 3702 4573 5346 3702 Size: 871 773 6112 Fragments arranged by size: 6112 871 773 KpnI G_GTAC'C Cuts at: 4241 5081 4241 Size: 840 6916 MfeI C'AATT_G Cuts at: 5355 5355 Size: 7756 MluI A'CGCG_T Cuts at: 7632 7632 Size: 7756 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2679 6870 7054 1305 Size: 1374 4191 184 2007 Fragments arranged by size: 4191 2007 1374 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2031 4649 10 Size: 54 1425 343 199 2618 3117 Fragments arranged by size: 3117 2618 1425 343 199 54 MslI CAynn'nnrTG Cuts at: 518 1166 2754 2789 2954 4091 4132 4136 4370 Size: 648 1588 35 165 1137 41 4 234 Cuts at: 4370 5714 6073 6232 518 Size: 1344 359 159 2042 Fragments arranged by size: 2042 1588 1344 1137 648 359 234 165 159 41 35 4 MspA1I CmG'CkG Cuts at: 1606 3304 4006 4244 5172 5849 6997 7242 1606 Size: 1698 702 238 928 677 1148 245 2120 Fragments arranged by size: 2120 1698 1148 928 702 677 245 238 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 6265 Fragments arranged by size: 6265 939 552 NdeI CA'TA_TG Cuts at: 387 4158 387 Size: 3771 3985 NheI G'CTAG_C Cuts at: 1052 3520 1052 Size: 2468 5288 NotI GC'GGCC_GC Cuts at: 5203 5203 Size: 7756 NsiI A_TGCA'T Cuts at: 2305 2602 2305 Size: 297 7459 NspI r_CATG'y Cuts at: 1132 1229 2604 2959 3250 4417 4963 5114 6659 Size: 97 1375 355 291 1167 546 151 1545 Cuts at: 6659 1132 Size: 2229 Fragments arranged by size: 2229 1545 1375 1167 546 355 291 151 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7756 PciI A'CATG_T Cuts at: 4959 6655 4959 Size: 1696 6060 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7756 PfoI T'CCnGG_A Cuts at: 4044 4044 Size: 7756 PmeI GTTT'AAAC Cuts at: 5060 5060 Size: 7756 PmlI CAC'GTG Cuts at: 2756 2756 Size: 7756 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5825 5857 7338 7370 5825 Size: 32 1481 32 6211 Fragments arranged by size: 6211 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2821 2821 Size: 7756 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7756 PsiI TTA'TAA Cuts at: 2525 5326 2525 Size: 2801 4955 PstI C_TGCA'G Cuts at: 830 2724 5108 830 Size: 1894 2384 3478 Fragments arranged by size: 3478 2384 1894 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7756 PvuII CAG'CTG Cuts at: 3304 4006 4244 3304 Size: 702 238 6816 Fragments arranged by size: 6816 702 238 SacI G_AGCT'C Cuts at: 2487 2487 Size: 7756 SalI G'TCGA_C Cuts at: 1936 5098 1936 Size: 3162 4594 SapI GCTCTTCn'nnn_ Cuts at: 4789 4789 Size: 7756 SbfI CC_TGCA'GG Cuts at: 2724 5108 2724 Size: 2384 5372 ScaI AGT'ACT Cuts at: 6004 7716 6004 Size: 1712 6044 SexAI A'CCwGG_T Cuts at: 4032 4032 Size: 7756 SfcI C'TryA_G Cuts at: 826 1045 2283 2535 2720 3299 3811 4884 5104 Size: 219 1238 252 185 579 512 1073 220 Cuts at: 5104 6239 6920 7111 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1238 1135 1073 681 579 512 252 220 219 191 185 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7756 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7756 SmaI CCC'GGG Cuts at: 1872 5083 1872 Size: 3211 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3134 5860 6761 7023 820 Size: 197 926 1191 2726 901 262 1553 Fragments arranged by size: 2726 1553 1191 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5547 493 Size: 5054 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7756 SphI G_CATG'C Cuts at: 1132 5114 1132 Size: 3982 3774 SspI AAT'ATT Cuts at: 5 52 5680 5 Size: 47 5628 2081 Fragments arranged by size: 5628 2081 47 StuI AGG'CCT Cuts at: 1517 4080 1517 Size: 2563 5193 StyI C'CwwG_G Cuts at: 513 1065 2004 3171 4768 5195 513 Size: 552 939 1167 1597 427 3074 Fragments arranged by size: 3074 1597 1167 939 552 427 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5783 5800 5953 6138 6583 325 Size: 1255 4203 17 153 185 445 1498 Fragments arranged by size: 4203 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2564 4855 6002 Size: 274 80 33 51 1443 587 2291 1147 Cuts at: 6002 7714 96 Size: 1712 138 Fragments arranged by size: 2291 1712 1443 1147 587 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3004 3036 3078 3110 7716 7748 3004 Size: 32 42 32 4606 32 3012 Fragments arranged by size: 4606 3012 42 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3107 1316 Size: 1791 5965 XbaI T'CTAG_A Cuts at: 5092 5092 Size: 7756 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7756 XmaI C'CCGG_G Cuts at: 1870 5081 1870 Size: 3211 4545 XmnI GAAnn'nnTTC Cuts at: 2706 4879 5017 5885 2706 Size: 2173 138 868 4577 Fragments arranged by size: 4577 2173 868 138 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5564 276 Size: 53 83 186 936 4030 2468 Fragments arranged by size: 4030 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI XcmI