(Linear) (Six-base) MAPSORT of: KIEE9436.seq Check: 7850 from: 1 to: 3051 KIEE9436.orf With 182 enzymes: SgfI * March 22, 2012 18:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 159 1560 3051 Size: 159 1401 1491 Fragments arranged by size: 1491 1401 159 Acc65I G'GTAC_C Cuts at: 0 2232 3051 Size: 2232 819 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 62 688 902 1402 3051 Size: 62 626 214 500 1649 Fragments arranged by size: 1649 626 500 214 62 AflIII A'CryG_T Cuts at: 0 2954 3051 Size: 2954 97 AhdI GACnn_n'nnGTC Cuts at: 0 1322 3051 Size: 1322 1729 AlwNI CAG_nnn'CTG Cuts at: 0 2092 3051 Size: 2092 959 ApoI r'AATT_y Cuts at: 0 2209 2302 2709 2784 3051 Size: 2209 93 407 75 267 Fragments arranged by size: 2209 407 267 93 75 AvaI C'yCGr_G Cuts at: 0 794 1680 3051 Size: 794 886 1371 Fragments arranged by size: 1371 886 794 BamHI G'GATC_C Cuts at: 0 720 1566 2290 3051 Size: 720 846 724 761 Fragments arranged by size: 846 761 724 720 BanI G'GyrC_C Cuts at: 0 433 789 1006 2232 3051 Size: 433 356 217 1226 819 Fragments arranged by size: 1226 819 433 356 217 BanII G_rGCy'C Cuts at: 0 482 2469 3051 Size: 482 1987 582 Fragments arranged by size: 1987 582 482 BbsI GAAGACnn'nnnn_ Cuts at: 0 79 2025 3051 Size: 79 1946 1026 Fragments arranged by size: 1946 1026 79 BciVI GTATCCnnnnn_n' Cuts at: 0 659 3051 Size: 659 2392 BclI T'GATC_A Cuts at: 0 1202 1355 1592 3051 Size: 1202 153 237 1459 Fragments arranged by size: 1459 1202 237 153 BfrBI ATG'CAT Cuts at: 0 298 595 3051 Size: 298 297 2456 Fragments arranged by size: 2456 298 297 BglI GCCn_nnn'nGGC Cuts at: 0 32 1255 3051 Size: 32 1223 1796 Fragments arranged by size: 1796 1223 32 BlpI GC'TnA_GC Cuts at: 0 839 3051 Size: 839 2212 BmrI ACTGGGnnnn_n' Cuts at: 0 778 2470 2533 3051 Size: 778 1692 63 518 Fragments arranged by size: 1692 778 518 63 BmtI G_CTAG'C Cuts at: 0 1519 3051 Size: 1519 1532 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1602 3051 Size: 1602 1449 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 182 464 830 1198 1597 2739 3051 Size: 182 282 366 368 399 1142 312 Fragments arranged by size: 1142 399 368 366 312 282 182 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1150 3051 Size: 1150 1901 BsaI GGTCTCn'nnnn_ Cuts at: 0 2124 2901 3051 Size: 2124 777 150 Fragments arranged by size: 2124 777 150 BsaAI yAC'GTr Cuts at: 0 751 3051 Size: 751 2300 BsaHI Gr'CG_yC Cuts at: 0 305 3051 Size: 305 2746 BsaWI w'CCGG_w Cuts at: 0 1967 2319 3051 Size: 1967 352 732 Fragments arranged by size: 1967 732 352 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 999 1029 1425 1455 3051 Size: 999 30 396 30 1596 Fragments arranged by size: 1596 999 396 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 174 1757 3051 Size: 174 1583 1294 Fragments arranged by size: 1583 1294 174 BseYI C'CCAG_C Cuts at: 0 911 2468 3051 Size: 911 1557 583 Fragments arranged by size: 1557 911 583 BsiHKAI G_wGCw'C Cuts at: 0 482 3051 Size: 482 2569 BsmI GAATG_Cn' Cuts at: 0 597 1371 2146 3051 Size: 597 774 775 905 Fragments arranged by size: 905 775 774 597 Bsp1286I G_dGCh'C Cuts at: 0 482 2469 3051 Size: 482 1987 582 Fragments arranged by size: 1987 582 482 BspEI T'CCGG_A Cuts at: 0 1967 3051 Size: 1967 1084 BspHI T'CATG_A Cuts at: 0 1503 3051 Size: 1503 1548 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 159 722 1560 3051 Size: 159 563 838 1491 Fragments arranged by size: 1491 838 563 159 BsrDI GCAATG_nn' Cuts at: 0 354 2671 2988 3051 Size: 354 2317 317 63 Fragments arranged by size: 2317 354 317 63 BsrGI T'GTAC_A Cuts at: 0 559 3051 Size: 559 2492 BssSI C'ACGA_G Cuts at: 0 2046 3051 Size: 2046 1005 BstXI CCAn_nnnn'nTGG Cuts at: 0 751 786 3051 Size: 751 35 2265 Fragments arranged by size: 2265 751 35 BstYI r'GATC_y Cuts at: 0 720 1566 1964 2290 3051 Size: 720 846 398 326 761 Fragments arranged by size: 846 761 720 398 326 Bsu36I CC'TnA_GG Cuts at: 0 2494 3051 Size: 2494 557 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1463 3051 Size: 1463 1588 BtsI GCAGTG_nn' Cuts at: 0 25 645 2824 3051 Size: 25 620 2179 227 Fragments arranged by size: 2179 620 227 25 DraI TTT'AAA Cuts at: 0 2744 3051 Size: 2744 307 EaeI y'GGCC_r Cuts at: 0 24 2642 3051 Size: 24 2618 409 Fragments arranged by size: 2618 409 24 EarI CTCTTCn'nnn_ Cuts at: 0 683 981 1475 2784 3051 Size: 683 298 494 1309 267 Fragments arranged by size: 1309 683 494 298 267 EcoICRI GAG'CTC Cuts at: 0 480 3051 Size: 480 2571 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 62 182 464 688 830 902 1198 1402 Size: 62 120 282 224 142 72 296 204 Cuts at: 1402 1597 2739 3051 Size: 195 1142 312 Fragments arranged by size: 1142 312 296 282 224 204 195 142 120 72 62 EcoO109I rG'GnC_Cy Cuts at: 0 816 3051 Size: 816 2235 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 527 3051 Size: 527 2524 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 359 391 1187 1219 1308 1340 2661 2693 Size: 359 32 796 32 89 32 1321 32 Cuts at: 2693 3051 Size: 358 Fragments arranged by size: 1321 796 359 358 89 32 32 32 32 HincII GTy'rAC Cuts at: 0 1068 1107 1697 2568 3051 Size: 1068 39 590 871 483 Fragments arranged by size: 1068 871 590 483 39 HindIII A'AGCT_T Cuts at: 0 237 700 1821 3027 3051 Size: 237 463 1121 1206 24 Fragments arranged by size: 1206 1121 463 237 24 HpaI GTT'AAC Cuts at: 0 1697 2568 3051 Size: 1697 871 483 Fragments arranged by size: 1697 871 483 KpnI G_GTAC'C Cuts at: 0 2236 3051 Size: 2236 815 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 674 3051 Size: 674 2377 MscI TGG'CCA Cuts at: 0 26 2644 3051 Size: 26 2618 407 Fragments arranged by size: 2618 407 26 MslI CAynn'nnrTG Cuts at: 0 749 784 949 2086 2127 2131 2365 3051 Size: 749 35 165 1137 41 4 234 686 Fragments arranged by size: 1137 749 686 234 165 41 35 4 MspA1I CmG'CkG Cuts at: 0 1299 2001 2239 3051 Size: 1299 702 238 812 Fragments arranged by size: 1299 812 702 238 NdeI CA'TA_TG Cuts at: 0 2153 3051 Size: 2153 898 NheI G'CTAG_C Cuts at: 0 1515 3051 Size: 1515 1536 NsiI A_TGCA'T Cuts at: 0 300 597 3051 Size: 300 297 2454 Fragments arranged by size: 2454 300 297 NspI r_CATG'y Cuts at: 0 599 954 1245 2412 2958 3051 Size: 599 355 291 1167 546 93 Fragments arranged by size: 1167 599 546 355 291 93 PciI A'CATG_T Cuts at: 0 2954 3051 Size: 2954 97 PfoI T'CCnGG_A Cuts at: 0 2039 3051 Size: 2039 1012 PmlI CAC'GTG Cuts at: 0 751 3051 Size: 751 2300 PpuMI rG'GwC_Cy Cuts at: 0 816 3051 Size: 816 2235 PsiI TTA'TAA Cuts at: 0 520 3051 Size: 520 2531 PstI C_TGCA'G Cuts at: 0 719 3051 Size: 719 2332 PvuII CAG'CTG Cuts at: 0 1299 2001 2239 3051 Size: 1299 702 238 812 Fragments arranged by size: 1299 812 702 238 SacI G_AGCT'C Cuts at: 0 482 3051 Size: 482 2569 SapI GCTCTTCn'nnn_ Cuts at: 0 2784 3051 Size: 2784 267 SbfI CC_TGCA'GG Cuts at: 0 719 3051 Size: 719 2332 SexAI A'CCwGG_T Cuts at: 0 2027 3051 Size: 2027 1024 SfcI C'TryA_G Cuts at: 0 278 530 715 1294 1806 2879 3051 Size: 278 252 185 579 512 1073 172 Fragments arranged by size: 1073 579 512 278 252 185 172 SmlI C'TyrA_G Cuts at: 0 1129 3051 Size: 1129 1922 StuI AGG'CCT Cuts at: 0 2075 3051 Size: 2075 976 StyI C'CwwG_G Cuts at: 0 1166 2763 3051 Size: 1166 1597 288 Fragments arranged by size: 1597 1166 288 TatI w'GTAC_w Cuts at: 0 559 2850 3051 Size: 559 2291 201 Fragments arranged by size: 2291 559 201 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 999 1031 1073 1105 3051 Size: 999 32 42 32 1946 Fragments arranged by size: 1946 999 42 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1102 3051 Size: 1102 1949 XmnI GAAnn'nnTTC Cuts at: 0 701 2874 3012 3051 Size: 701 2173 138 39 Fragments arranged by size: 2173 701 138 39 Enzymes that do cut: AarI Acc65I AcuI AflIII AhdI AlwNI ApoI AvaI BamHI BanI BanII BbsI BciVI BclI BfrBI BglI BlpI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrDI BsrGI BssSI BstXI BstYI Bsu36I BtgZI BtsI DraI EaeI EarI EcoICRI Eco57MI EcoO109I FalI Hin4I HincII HindIII HpaI KpnI MmeI MscI MslI MspA1I NdeI NheI NsiI NspI PciI PfoI PmlI PpuMI PsiI PstI PvuII SacI SapI SbfI SexAI SfcI SmlI StuI StyI TatI TstI Tth111I XmnI Enzymes that do not cut: AatII AccI AclI AfeI AflII AgeI AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BbeI BbvCI BcgI BglII Bme1580I BmgBI Bpu10I BsaBI BsgI BsiEI BsiWI BsmBI BsrBI BsrFI BssHII BstAPI BstBI BstEII BstZ17I BtgI ClaI CspCI DraIII DrdI EagI EciI EcoNI EcoRI EcoRV FseI FspI FspAI HaeII KasI MfeI MluI NaeI NarI NcoI NgoMIV NotI NruI PacI PasI PflMI PmeI PpiI PshAI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TaqII XbaI XcmI XhoI XmaI ZraI