################################## # NO CUT ENZYMES ( 190 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AclI AcyI AflII AgeI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BbvI BcgI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstPI BstUI BstXI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DsaI EaeI EagI EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoT22I FauI FbaI Fnu4HI FseI FspI HaeII HaeIII HapII HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII HphI ItaI KasI KpnI Ksp632I KspI MaeI MaeII MamI MfeI MluI MluNI MroI MscI MspI MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI Sau96I SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI TseI Tsp45I Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AflIII 625 ACATGT A^C[GA][CT]GT AhdI 30 GACTTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 754 GATCC GGATC(4/5) AspEI 30 GACTTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BciVI 232 GGATAC GTATCC(6/5) BsaJI 133 CCAAGG C^C[ACGT][ACGT]GG BsiYI 430 CCATTAAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 430 CCATTAAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BspLU11I 625 ACATGT A^CATGT BsrDI 210 CATTGC GCAATG(2/0) BssKI 384 CCTGG ^CC[ACGT]GG Bst1107I 415 GTATAC GTA^TAC BstNI 384 CCTGG CC^[AT]GG BstYI 753 AGATCC [GA]^GATC[CT] BstZ17I 415 GTATAC GTA^TAC DrdI 468 GACAAGACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 30 GACTTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRII 384 CCTGG ^CC[AT]GG EcoRV 116 GATATC GAT^ATC EcoT14I 133 CCAAGG C^C[AT][AT]GG HgaI 190 GACGC GACGC(5/10) HinfI 795 GAATC G^A[ACGT]TC MflI 753 AGATCC [GA]^GATC[CT] MspA1I 685 CAGCTG C[AC]G^C[GT]G MvaI 384 CCTGG CC^[AT]GG NspI 625 ACATGT [GA]CATG^[CT] PflMI 430 CCATTAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 685 CAGCTG CAG^CTG ScaI 555 AGTACT AGT^ACT ScrFI 384 CCTGG CC^[ACGT]GG SfcI 94 CTACAG C^T[GA][CT]AG SmlI 343 CTCAAG C^T[CT][GA]AG SspI 831 AATATT AAT^ATT StyI 133 CCAAGG C^C[AT][AT]GG TfiI 795 GAATC G^A[AT]TC Van91I 430 CCATTAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 620 CCAAAACATGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 753 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### AccI 415 GTATAC GT^[AC][GT]AC 857 GTCTAC AluI 462 AGCT AG^CT 686 AGCT Asp700I 334 GAATTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 830 GAATATTTTC BbsI 348 GAAGAC GAAGAC(2/6) 698 GAAGAC BpuAI 348 GAAGAC GAAGAC(2/6) 698 GAAGAC MaeIII 125 GTTAC ^GT[ACGT]AC 380 GTAAC MslI 389 CACTGATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 610 CATGAAGATG SfaNI 616 GATGC GCATC(5/9) 440 GCATC TaqI 260 TCGA T^CGA 568 TCGA TthHB8I 260 TCGA T^CGA 568 TCGA XmnI 334 GAATTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 830 GAATATTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 19 enzymes) # ######################################### AcsI AfaI ApoI BsrI BstF5I DdeI DpnII FokI MboI MboII MnlI MseI NdeII NlaIII RsaI Sau3AI Tru9I Tsp509I TspRI