(Circular) (Six-base) MAPSORT of: pFN21AE0432.seq Check: 9536 from: 1 to: 5923 pFN21AE0432 5923 bp With 182 enzymes: SgfI * October 20, 2009 10:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3733 278 Size: 53 83 186 936 2197 2468 Fragments arranged by size: 2468 2197 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3266 1937 Size: 1329 4594 Acc65I G'GTAC_C Cuts at: 2851 3244 2851 Size: 393 5530 AclI AA'CG_TT Cuts at: 1563 4050 4423 1563 Size: 2487 373 3063 Fragments arranged by size: 3063 2487 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2073 2547 3986 5370 5638 1632 Size: 441 474 1439 1384 268 1917 Fragments arranged by size: 1917 1439 1384 474 441 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5726 AflIII A'CryG_T Cuts at: 1203 2038 2834 4822 5799 1203 Size: 835 796 1988 977 1327 Fragments arranged by size: 1988 1327 977 835 796 AgeI A'CCGG_T Cuts at: 4754 4754 Size: 5923 AhdI GACnn_n'nnGTC Cuts at: 4652 4652 Size: 5923 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5923 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5923 AlwNI CAG_nnn'CTG Cuts at: 2654 2711 3666 5238 2654 Size: 57 955 1572 3339 Fragments arranged by size: 3339 1572 955 57 ApaLI G'TGCA_C Cuts at: 3980 5136 3980 Size: 1156 4767 ApoI r'AATT_y Cuts at: 1493 2071 2364 2681 2781 2919 3232 3458 3610 Size: 578 293 317 100 138 313 226 152 Cuts at: 3610 5719 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 578 317 313 293 226 152 138 100 AseI AT'TA_AT Cuts at: 160 1032 4477 160 Size: 872 3445 1606 Fragments arranged by size: 3445 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5923 AvaI C'yCGr_G Cuts at: 1870 1943 2730 3248 1870 Size: 73 787 518 4545 Fragments arranged by size: 4545 787 518 73 BamHI G'GATC_C Cuts at: 3253 3620 3253 Size: 367 5556 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2851 3244 4699 618 Size: 325 217 627 1064 393 1455 1842 Fragments arranged by size: 1842 1455 1064 627 393 325 217 BanII G_rGCy'C Cuts at: 1391 3242 1391 Size: 1851 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2364 928 Size: 971 465 4487 Fragments arranged by size: 4487 971 465 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4114 4148 1903 Size: 34 2177 34 3678 Fragments arranged by size: 3678 2177 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3815 5025 3815 Size: 1210 4713 BclI T'GATC_A Cuts at: 1548 2494 1548 Size: 946 4977 BfrBI ATG'CAT Cuts at: 2908 2908 Size: 5923 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2732 2741 4534 Size: 107 122 71 631 605 1060 9 1793 Cuts at: 4534 136 Size: 1525 Fragments arranged by size: 1793 1525 1060 631 605 122 107 71 9 BglII A'GATC_T Cuts at: 5918 5918 Size: 5923 BlpI GC'TnA_GC Cuts at: 3340 3340 Size: 5923 Bme1580I G_kGCm'C Cuts at: 1790 2525 3074 3984 5140 1790 Size: 735 549 910 1156 2573 Fragments arranged by size: 2573 1156 910 735 549 BmgBI CAC'GTC Cuts at: 1318 2039 1318 Size: 721 5202 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3654 4612 442 Size: 416 53 83 399 24 2237 958 1753 Fragments arranged by size: 2237 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5923 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3050 4583 1239 Size: 1811 1533 2579 Fragments arranged by size: 2579 1811 1533 Bpu10I CC'TnA_GC Cuts at: 2359 2359 Size: 5923 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4048 4913 5211 4048 Size: 865 298 4760 Fragments arranged by size: 4760 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4586 882 Size: 617 3087 2219 Fragments arranged by size: 3087 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2280 3714 493 Size: 711 1076 1434 2702 Fragments arranged by size: 2702 1434 1076 711 BsaBI GATnn'nnATC Cuts at: 5917 5917 Size: 5923 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3730 4112 4747 275 Size: 53 83 186 936 2197 382 635 1451 Fragments arranged by size: 2197 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4356 4754 5028 5175 5586 4356 Size: 398 274 147 411 4693 Fragments arranged by size: 4693 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2711 2741 1178 Size: 30 165 30 1308 30 4360 Fragments arranged by size: 4360 1308 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2122 3224 1186 Size: 936 1102 3885 Fragments arranged by size: 3885 1102 936 BseYI C'CCAG_C Cuts at: 5126 5622 5126 Size: 496 5427 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2381 2697 1163 Size: 573 645 316 4389 Fragments arranged by size: 4389 645 573 316 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3373 4134 5162 1540 Size: 275 186 1372 761 1028 2301 Fragments arranged by size: 2301 1372 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3984 4069 5140 3984 Size: 85 1071 4767 Fragments arranged by size: 4767 1071 85 BsmI GAATG_Cn' Cuts at: 2462 3434 3527 2462 Size: 972 93 4858 Fragments arranged by size: 4858 972 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2029 2029 Size: 5923 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2525 3074 3242 3984 4069 5140 1391 Size: 399 735 549 168 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 735 549 399 168 85 BspEI T'CCGG_A Cuts at: 5586 5586 Size: 5923 BspHI T'CATG_A Cuts at: 3812 5542 3812 Size: 1730 4193 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3278 844 Size: 2434 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2116 2155 3810 1394 Size: 429 293 39 1655 3507 Fragments arranged by size: 3507 1655 429 293 39 BsrDI GCAATG_nn' Cuts at: 65 1248 2052 4418 4592 65 Size: 1183 804 2366 174 1396 Fragments arranged by size: 2366 1396 1183 804 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4567 4754 1700 Size: 110 2757 187 2869 Fragments arranged by size: 2869 2757 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5923 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5923 BssSI C'ACGA_G Cuts at: 3983 4995 3983 Size: 1012 4911 BstAPI GCAn_nnn'nTGC Cuts at: 794 2711 794 Size: 1917 4006 BstBI TT'CG_AA Cuts at: 4727 4727 Size: 5923 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5923 BstYI r'GATC_y Cuts at: 1972 3253 3620 4005 4022 5474 5560 5572 5918 Size: 1281 367 385 17 1452 86 12 346 Cuts at: 5918 1972 Size: 1977 Fragments arranged by size: 1977 1452 1281 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3162 513 Size: 552 939 1158 3274 Fragments arranged by size: 3274 1158 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5184 Fragments arranged by size: 5184 683 56 BtsI GCAGTG_nn' Cuts at: 3439 4233 4253 3439 Size: 794 20 5109 Fragments arranged by size: 5109 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5923 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5888 DraI TTT'AAA Cuts at: 3227 3227 Size: 5923 DraIII CAC_nnn'GTG Cuts at: 2953 2953 Size: 5923 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4930 809 Size: 507 3614 1802 Fragments arranged by size: 3614 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2628 3370 4259 Size: 54 1425 50 275 18 798 742 889 Cuts at: 4259 5888 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 798 742 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3370 1537 Size: 275 1558 4090 Fragments arranged by size: 4090 1558 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2092 2099 2511 2664 2682 3853 1353 Size: 739 7 412 153 18 1171 3423 Fragments arranged by size: 3423 1171 739 412 153 18 7 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4510 4884 5030 387 Size: 1064 138 2921 374 146 1280 Fragments arranged by size: 2921 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2073 2547 3050 3986 4583 5370 5638 Size: 393 441 474 503 936 597 787 268 Cuts at: 5638 1239 Size: 1524 Fragments arranged by size: 1524 936 787 597 503 474 441 393 268 EcoRI G'AATT_C Cuts at: 3232 3610 3232 Size: 378 5545 FspI TGC'GCA Cuts at: 4429 4429 Size: 5923 HaeII r_GCGC'y Cuts at: 1678 5070 1678 Size: 3392 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2139 2171 2491 2523 2711 Size: 32 135 32 567 32 320 32 188 Cuts at: 2711 2743 4564 4596 4638 4670 1373 Size: 32 1821 32 42 32 2626 Fragments arranged by size: 2626 1821 567 320 188 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3267 3513 669 Size: 988 281 1329 246 3079 Fragments arranged by size: 3079 1329 988 281 246 HindIII A'AGCT_T Cuts at: 748 3047 748 Size: 2299 3624 HpaI GTT'AAC Cuts at: 3513 3513 Size: 5923 KpnI G_GTAC'C Cuts at: 2855 3248 2855 Size: 393 5530 MfeI C'AATT_G Cuts at: 3522 3522 Size: 5923 MluI A'CGCG_T Cuts at: 5799 5799 Size: 5923 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2749 5037 5221 1305 Size: 1444 2288 184 2007 Fragments arranged by size: 2288 2007 1444 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2630 10 Size: 54 1425 343 798 3303 Fragments arranged by size: 3303 1425 798 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2064 2240 3881 4240 4399 518 Size: 648 898 176 1641 359 159 2042 Fragments arranged by size: 2042 1641 898 648 359 176 159 MspA1I CmG'CkG Cuts at: 1606 3196 3339 4016 5164 5409 1606 Size: 1590 143 677 1148 245 2120 Fragments arranged by size: 2120 1590 1148 677 245 143 NcoI C'CATG_G Cuts at: 513 1065 2004 3162 513 Size: 552 939 1158 3274 Fragments arranged by size: 3274 1158 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5923 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5923 NotI GC'GGCC_GC Cuts at: 3370 3370 Size: 5923 NsiI A_TGCA'T Cuts at: 2910 2910 Size: 5923 NspI r_CATG'y Cuts at: 1132 1229 2584 2705 3281 4826 1132 Size: 97 1355 121 576 1545 2229 Fragments arranged by size: 2229 1545 1355 576 121 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5923 PciI A'CATG_T Cuts at: 4822 4822 Size: 5923 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5923 PfoI T'CCnGG_A Cuts at: 3098 3098 Size: 5923 PmeI GTTT'AAAC Cuts at: 3227 3227 Size: 5923 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3992 4024 5505 5537 3992 Size: 32 1481 32 4378 Fragments arranged by size: 4378 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5923 PsiI TTA'TAA Cuts at: 2930 3493 2930 Size: 563 5360 PstI C_TGCA'G Cuts at: 830 3009 3275 830 Size: 2179 266 3478 Fragments arranged by size: 3478 2179 266 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5923 SalI G'TCGA_C Cuts at: 1936 3265 1936 Size: 1329 4594 SbfI CC_TGCA'GG Cuts at: 3275 3275 Size: 5923 ScaI AGT'ACT Cuts at: 4171 5883 4171 Size: 1712 4211 SfcI C'TryA_G Cuts at: 826 1045 3005 3271 4406 5087 5278 826 Size: 219 1960 266 1135 681 191 1471 Fragments arranged by size: 1960 1471 1135 681 266 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5923 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5923 SmaI CCC'GGG Cuts at: 1872 2732 3250 1872 Size: 860 518 4545 Fragments arranged by size: 4545 860 518 SmlI C'TyrA_G Cuts at: 820 1017 1943 4027 4928 5190 820 Size: 197 926 2084 901 262 1553 Fragments arranged by size: 2084 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2280 3714 493 Size: 1787 1434 2702 Fragments arranged by size: 2702 1787 1434 SpeI A'CTAG_T Cuts at: 152 2755 152 Size: 2603 3320 SphI G_CATG'C Cuts at: 1132 3281 1132 Size: 2149 3774 SspI AAT'ATT Cuts at: 5 52 2134 3847 5 Size: 47 2082 1713 2081 Fragments arranged by size: 2082 2081 1713 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5923 StyI C'CwwG_G Cuts at: 513 1065 2004 3162 3362 513 Size: 552 939 1158 200 3074 Fragments arranged by size: 3074 1158 939 552 200 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3950 3967 4120 4305 4750 325 Size: 1255 2370 17 153 185 445 1498 Fragments arranged by size: 2370 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2758 4169 5881 Size: 274 80 33 51 1443 781 1411 1712 Cuts at: 5881 96 Size: 138 Fragments arranged by size: 1712 1443 1411 781 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5883 5915 5883 Size: 32 5891 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5923 XbaI T'CTAG_A Cuts at: 3259 3259 Size: 5923 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5923 XmaI C'CCGG_G Cuts at: 1870 2730 3248 1870 Size: 860 518 4545 Fragments arranged by size: 4545 860 518 XmnI GAAnn'nnTTC Cuts at: 4052 4052 Size: 5923 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3731 276 Size: 53 83 186 936 2197 2468 Fragments arranged by size: 2468 2197 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI