################################## # NO CUT ENZYMES ( 122 enzymes) # ################################## AatII AccI AccIII AclI AcyI AhdI Alw44I AocI Aor51HI ApaLI AscI AspEI AspHI AspI AvaI AviII AvrII BamHI BbeI BbvCI BcgI BcnI BglI BglII BlnI BlpI BpmI Bpu1102I BsaBI BsaHI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrGI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstEII BstNI BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRII FseI FspI Hin1I KasI KspI MamI MfeI MluI MroI MslI MunI MvaI NaeI NarI NciI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PmeI PpuMI PshAI Psp1406I PvuI RsrII SacI SacII SalI SbfI ScaI ScrFI SexAI SfiI SfoI SfuI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccII 1342 CGCG CG^CG AflIII 1510 ACACGT A^C[GA][CT]GT AgeI 448 ACCGGT A^CCGGT ApaI 5 GGGCCC GGGCC^C AseI 2059 ATTAAT AT^TAAT AsnI 2059 ATTAAT AT^TAAT AvaII 1628 GGTCC G^G[AT]CC BbrPI 1181 CACGTG CAC^GTG BciVI 783 GGATAC GTATCC(6/5) BsaAI 1181 CACGTG [CT]AC^GT[GA] BsaI 1032 GAGACC GGTCTC(1/5) BsaWI 448 ACCGGT [AT]^CCGG[AT] BseRI 868 GAGGAG GAGGAG(10/8) BsmI 2222 GAATGC GAATGC(1/-1) BspHI 1982 TCATGA T^CATGA BspMI 428 ACCTGC ACCTGC(4/8) BsrFI 448 ACCGGT [GA]^CCGG[CT] BstUI 1342 CGCG CG^CG Cac8I 1945 GCCAGC GC[ACGT]^[ACGT]GC Cfr10I 448 ACCGGT [GA]^CCGG[CT] DraI 522 TTTAAA TTT^AAA DrdI 42 GACCTTCGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRV 1012 GATATC GAT^ATC EcoT22I 185 ATGCAT ATGCA^T HaeII 1913 AGCGCC [GA]GCGC^[CT] HapII 449 CCGG C^CGG HgaI 32 GCGTC GACGC(5/10) HpaI 244 GTTAAC GTT^AAC HpaII 449 CCGG C^CGG HphI 1374 GGTGA GGTGA(8/7) MspI 449 CCGG C^CGG MvnI 1342 CGCG CG^CG NdeI 2201 CATATG CA^TATG NsiI 185 ATGCAT ATGCA^T PflMI 1575 CCAGTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 448 ACCGGT A^CCGGT PmaCI 1181 CACGTG CAC^GTG PmlI 1181 CACGTG CAC^GTG PshBI 2059 ATTAAT AT^TAAT PstI 929 CTGCAG CTGCA^G PvuII 2390 CAGCTG CAG^CTG RcaI 1982 TCATGA T^CATGA SapI 2269 GAAGAGC GCTCTTC(1/4) SgrAI 447 CACCGGTG C[GA]^CCGG[CT]G SspI 809 AATATT AAT^ATT TspRI 356 CACTG CA[GC]TG[ACGT][ACGT]^ Van91I 1575 CCAGTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 464 CCAGAGAAGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### Acc65I 490 GGTACC G^GTACC 1465 GGTACC AflII 1369 CTTAAG C^TTAAG 1879 CTTAAG AlwNI 1706 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG 1912 CAGCGCCTG Asp700I 160 GAAGCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1755 GAAGAGGTTC Asp718I 490 GGTACC G^GTACC 1465 GGTACC BanI 490 GGTACC G^G[CT][GA]CC 1465 GGTACC BclI 1412 TGATCA T^GATCA 2078 TGATCA BfrI 1369 CTTAAG C^TTAAG 1879 CTTAAG BsmFI 508 GGGAC GGGAC(10/14) 992 GGGAC CfoI 1239 GCGC GCG^C 1914 GCGC DsaI 875 CCATGG C^C[GA][CT]GG 1106 CCATGG FauI 1340 CCCGC CCCGC(4/6) 2073 CCCGC FbaI 1412 TGATCA T^GATCA 2078 TGATCA HhaI 1239 GCGC GCG^C 1914 GCGC HinP1I 1239 GCGC G^CGC 1914 GCGC HincII 244 GTTAAC GT[CT]^[GA]AC 558 GTCAAC HindII 244 GTTAAC GT[CT]^[GA]AC 558 GTCAAC KpnI 490 GGTACC GGTAC^C 1465 GGTACC MaeII 1182 ACGT A^CGT 1512 ACGT MspA1I 2074 CCGCTG C[AC]G^C[GT]G 2390 CAGCTG NcoI 875 CCATGG C^CATGG 1106 CCATGG NspI 435 GCATGT [GA]CATG^[CT] 2087 ACATGC SfcI 929 CTGCAG C^T[GA][CT]AG 2387 CTACAG SmlI 1369 CTTAAG C^T[CT][GA]AG 1879 CTTAAG Tsp45I 439 GTCAC ^GT[GC]AC 631 GTCAC XmnI 160 GAAGCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1755 GAAGAGGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 62 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI BalI BanII BbsI BbvI BfaI BmyI BpuAI BsaJI BsiYI BslI BsmAI Bsp1286I BsrI BstF5I BstYI Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoT14I Fnu4HI FokI HaeIII HindIII HinfI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MwoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I SfaNI StyI TaqI TfiI Tru9I TseI Tsp509I TthHB8I XhoII