Restriction map for
pFN21AE1978
(Linear) (Six-base) MAPSORT of: KIEE1978.seq Check: 7550 from: 1 to: 1665
KIEE1978.orf
With 182 enzymes: SgfI *
June 30, 2019 14:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 223 730 1606 1665
Size: 223 507 876 59
Fragments arranged by size:
876 507 223 59
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 362 489 1665
Size: 362 127 1176
Fragments arranged by size:
1176 362 127
AlwNI CAG_nnn'CTG
Cuts at: 0 261 1101 1341 1665
Size: 261 840 240 324
Fragments arranged by size:
840 324 261 240
ApaI G_GGCC'C
Cuts at: 0 291 588 965 1089 1430 1665
Size: 291 297 377 124 341 235
Fragments arranged by size:
377 341 297 291 235 124
ApoI r'AATT_y
Cuts at: 0 1552 1665
Size: 1552 113
AvaI C'yCGr_G
Cuts at: 0 165 353 589 964 1166 1665
Size: 165 188 236 375 202 499
Fragments arranged by size:
499 375 236 202 188 165
BamHI G'GATC_C
Cuts at: 0 950 1665
Size: 950 715
BanI G'GyrC_C
Cuts at: 0 211 247 658 1483 1594 1665
Size: 211 36 411 825 111 71
Fragments arranged by size:
825 411 211 111 71 36
BanII G_rGCy'C
Cuts at: 0 172 291 298 588 746 965 1089 1116
Size: 172 119 7 290 158 219 124 27
Cuts at: 1116 1165 1430 1665
Size: 49 265 235
Fragments arranged by size:
290 265 235 219 172 158 124 119
49 27 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 487 1665
Size: 487 1178
BbvCI CC'TCA_GC
Cuts at: 0 1342 1665
Size: 1342 323
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1157 1191 1665
Size: 1157 34 474
Fragments arranged by size:
1157 474 34
BclI T'GATC_A
Cuts at: 0 1491 1665
Size: 1491 174
BglI GCCn_nnn'nGGC
Cuts at: 0 167 1665
Size: 167 1498
BlpI GC'TnA_GC
Cuts at: 0 1197 1665
Size: 1197 468
Bme1580I G_kGCm'C
Cuts at: 0 250 291 588 965 1089 1144 1430 1665
Size: 250 41 297 377 124 55 286 235
Fragments arranged by size:
377 297 286 250 235 124 55 41
BmgBI CAC'GTC
Cuts at: 0 147 402 1665
Size: 147 255 1263
Fragments arranged by size:
1263 255 147
BmrI ACTGGGnnnn_n'
Cuts at: 0 172 1665
Size: 172 1493
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 990 1665
Size: 990 675
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 1140 1270 1622 1665
Size: 238 902 130 352 43
Fragments arranged by size:
902 352 238 130 43
Bpu10I CC'TnA_GC
Cuts at: 0 410 1342 1665
Size: 410 932 323
Fragments arranged by size:
932 410 323
BsaI GGTCTCn'nnnn_
Cuts at: 0 1163 1665
Size: 1163 502
BsaAI yAC'GTr
Cuts at: 0 1041 1665
Size: 1041 624
BsaWI w'CCGG_w
Cuts at: 0 1646 1665
Size: 1646 19
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 7 853 1095 1604 1665
Size: 7 846 242 509 61
Fragments arranged by size:
846 509 242 61 7
BseYI C'CCAG_C
Cuts at: 0 623 684 1244 1665
Size: 623 61 560 421
Fragments arranged by size:
623 560 421 61
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 201 550 1021 1665
Size: 201 349 471 644
Fragments arranged by size:
644 471 349 201
BsiEI CG_ry'CG
Cuts at: 0 1138 1665
Size: 1138 527
BsiHKAI G_wGCw'C
Cuts at: 0 1354 1412 1665
Size: 1354 58 253
Fragments arranged by size:
1354 253 58
Bsp1286I G_dGCh'C
Cuts at: 0 172 250 291 298 588 746 965 1089
Size: 172 78 41 7 290 158 219 124
Cuts at: 1089 1116 1144 1165 1354 1412 1430 1665
Size: 27 28 21 189 58 18 235
Fragments arranged by size:
290 235 219 189 172 158 124 78
58 41 28 27 21 18 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 223 605 730 947 1431 1606 1665
Size: 223 382 125 217 484 175 59
Fragments arranged by size:
484 382 223 217 175 125 59
BsrBI CCG'CTC
Cuts at: 0 164 1665
Size: 164 1501
BsrDI GCAATG_nn'
Cuts at: 0 429 1665
Size: 429 1236
BsrFI r'CCGG_y
Cuts at: 0 442 1133 1665
Size: 442 691 532
Fragments arranged by size:
691 532 442
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1044 1665
Size: 1044 621
BstEII G'GTnAC_C
Cuts at: 0 1203 1254 1665
Size: 1203 51 411
Fragments arranged by size:
1203 411 51
BstXI CCAn_nnnn'nTGG
Cuts at: 0 298 1665
Size: 298 1367
BstYI r'GATC_y
Cuts at: 0 950 1665
Size: 950 715
Bsu36I CC'TnA_GG
Cuts at: 0 637 1432 1665
Size: 637 795 233
Fragments arranged by size:
795 637 233
BtgI C'CryG_G
Cuts at: 0 804 1063 1191 1301 1665
Size: 804 259 128 110 364
Fragments arranged by size:
804 364 259 128 110
BtsI GCAGTG_nn'
Cuts at: 0 707 1295 1665
Size: 707 588 370
Fragments arranged by size:
707 588 370
DraIII CAC_nnn'GTG
Cuts at: 0 1358 1665
Size: 1358 307
DrdI GACnn_nn'nnGTC
Cuts at: 0 388 1665
Size: 388 1277
EaeI y'GGCC_r
Cuts at: 0 128 440 690 915 1135 1332 1415 1542
Size: 128 312 250 225 220 197 83 127
Cuts at: 1542 1665
Size: 123
Fragments arranged by size:
312 250 225 220 197 128 127 123
83
EagI C'GGCC_G
Cuts at: 0 1135 1665
Size: 1135 530
EarI CTCTTCn'nnn_
Cuts at: 0 1153 1327 1665
Size: 1153 174 338
Fragments arranged by size:
1153 338 174
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 148 1665
Size: 148 1517
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 362 489 1140 1270 1622 1665
Size: 238 124 127 651 130 352 43
Fragments arranged by size:
651 352 238 130 127 124 43
EcoNI CCTnn'n_nnAGG
Cuts at: 0 241 636 1665
Size: 241 395 1029
Fragments arranged by size:
1029 395 241
EcoO109I rG'GnC_Cy
Cuts at: 0 584 585 594 1059 1085 1086 1236 1275
Size: 584 1 9 465 26 1 150 39
Cuts at: 1275 1426 1427 1665
Size: 151 1 238
Fragments arranged by size:
584 465 238 151 150 39 26 9
1 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1341 1665
Size: 1341 324
FspI TGC'GCA
Cuts at: 0 718 1665
Size: 718 947
HaeII r_GCGC'y
Cuts at: 0 261 274 312 897 1665
Size: 261 13 38 585 768
Fragments arranged by size:
768 585 261 38 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1441 1473 1665
Size: 1441 32 192
Fragments arranged by size:
1441 192 32
HincII GTy'rAC
Cuts at: 0 792 1665
Size: 792 873
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 674 1665
Size: 674 991
MscI TGG'CCA
Cuts at: 0 1334 1417 1544 1665
Size: 1334 83 127 121
Fragments arranged by size:
1334 127 121 83
MslI CAynn'nnrTG
Cuts at: 0 1179 1665
Size: 1179 486
MspA1I CmG'CkG
Cuts at: 0 564 1065 1201 1248 1665
Size: 564 501 136 47 417
Fragments arranged by size:
564 501 417 136 47
NaeI GCC'GGC
Cuts at: 0 444 1135 1665
Size: 444 691 530
Fragments arranged by size:
691 530 444
NcoI C'CATG_G
Cuts at: 0 1191 1665
Size: 1191 474
NgoMIV G'CCGG_C
Cuts at: 0 442 1133 1665
Size: 442 691 532
Fragments arranged by size:
691 532 442
NspI r_CATG'y
Cuts at: 0 433 1665
Size: 433 1232
PasI CC'CwG_GG
Cuts at: 0 291 1665
Size: 291 1374
PfoI T'CCnGG_A
Cuts at: 0 1638 1665
Size: 1638 27
PmlI CAC'GTG
Cuts at: 0 1041 1665
Size: 1041 624
PpuMI rG'GwC_Cy
Cuts at: 0 594 1059 1665
Size: 594 465 606
Fragments arranged by size:
606 594 465
PspOMI G'GGCC_C
Cuts at: 0 287 584 961 1085 1426 1665
Size: 287 297 377 124 341 239
Fragments arranged by size:
377 341 297 287 239 124
PstI C_TGCA'G
Cuts at: 0 1132 1665
Size: 1132 533
PvuII CAG'CTG
Cuts at: 0 1248 1665
Size: 1248 417
SacII CC_GC'GG
Cuts at: 0 1066 1665
Size: 1066 599
SapI GCTCTTCn'nnn_
Cuts at: 0 1153 1327 1665
Size: 1153 174 338
Fragments arranged by size:
1153 338 174
ScaI AGT'ACT
Cuts at: 0 973 1665
Size: 973 692
SfcI C'TryA_G
Cuts at: 0 238 534 1128 1665
Size: 238 296 594 537
Fragments arranged by size:
594 537 296 238
SmaI CCC'GGG
Cuts at: 0 355 1665
Size: 355 1310
StuI AGG'CCT
Cuts at: 0 832 1665
Size: 832 833
StyI C'CwwG_G
Cuts at: 0 827 1191 1479 1665
Size: 827 364 288 186
Fragments arranged by size:
827 364 288 186
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 637 676 1225 1665
Size: 637 39 549 440
Fragments arranged by size:
637 549 440 39
TatI w'GTAC_w
Cuts at: 0 497 971 1665
Size: 497 474 694
Fragments arranged by size:
694 497 474
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 179 211 1665
Size: 179 32 1454
Fragments arranged by size:
1454 179 32
Tth111I GACn'n_nGTC
Cuts at: 0 1609 1665
Size: 1609 56
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 345 1665
Size: 345 1320
XmaI C'CCGG_G
Cuts at: 0 353 1665
Size: 353 1312
XmnI GAAnn'nnTTC
Cuts at: 0 1321 1665
Size: 1321 344
Enzymes that do cut:
AarI AcuI AlwNI ApaI ApoI AvaI BamHI BanI
BanII BbsI BbvCI BcgI BclI BglI BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BsaI BsaAI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI
BsrDI BsrFI BstAPI BstEII BstXI BstYI Bsu36I BtgI
BtsI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I FalI FspI HaeII Hin4I HincII MmeI
MscI MslI MspA1I NaeI NcoI NgoMIV NspI PasI
PfoI PmlI PpuMI PspOMI PstI PvuII SacII SapI
ScaI SfcI SmaI StuI StyI TaqII TatI TstI
Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI
AvrII BaeI BbeI BciVI BfrBI BglII BmtI BpuEI
BsaBI BsaHI BsaXI BsiWI BsmI BsmBI BspEI BspHI
BsrGI BssHII BssSI BstBI BstZ17I BtgZI ClaI CspCI
DraI EcoICRI EcoRI EcoRV FseI FspAI HindIII HpaI
KasI KpnI MfeI MluI NarI NdeI NheI NotI
NruI NsiI PacI PciI PflMI PmeI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SalI SanDI
SbfI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI
SpeI SphI SrfI SspI SwaI XbaI XhoI ZraI