Restriction map for
pFN21AE2263
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# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccIII AcyI AflII
AgeI AhdI Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AviII AvrII BanI
BbeI BbvCI BcgI BclI BcnI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrGI BssSI Bst1107I BstAPI
BstBI BstXI BstZ17I CelII ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI MamI
MfeI MluI MroI MunI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PshAI PshBI PstI
PvuI RsrII SacI SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TaqI Tth111I TthHB8I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccI 725 GTCTAC GT^[AC][GT]AC
AclI 376 AACGTT AA^CGTT
AflIII 1131 ACACGT A^C[GA][CT]GT
AlwNI 1022 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1039 CCTGAGG CC^T[ACGT]AGG
BalI 740 TGGCCA TGG^CCA
BamHI 812 GGATCC G^GATCC
BbrPI 1132 CACGTG CAC^GTG
BbsI 976 GAAGAC GAAGAC(2/6)
BciVI 166 GTATCC GTATCC(6/5)
BglI 895 GCCCTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 976 GAAGAC GAAGAC(2/6)
BsaAI 1132 CACGTG [CT]AC^GT[GA]
BseRI 649 GAGGAG GAGGAG(10/8)
BsgI 214 CTGCAC GTGCAG(16/14)
BspHI 89 TCATGA T^CATGA
BsrDI 236 GCAATG GCAATG(2/0)
BsrFI 1090 ACCGGC [GA]^CCGG[CT]
BstEII 882 GGTCACC G^GT[ACGT]ACC
BstF5I 35 GGATG GGATG(2/0)
BstPI 882 GGTCACC G^GT[ACGT]ACC
BstYI 812 GGATCC [GA]^GATC[CT]
Bsu36I 1039 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1090 ACCGGC [GA]^CCGG[CT]
DpnII 813 GATC ^GATC
DsaI 40 CCGCGG C^C[GA][CT]GG
EarI 321 CTCTTC CTCTTC(1/4)
Eco81I 1039 CCTGAGG CC^T[ACGT]AGG
EcoO65I 882 GGTCACC G^GT[ACGT]ACC
EcoT14I 198 CCTTGG C^C[AT][AT]GG
FauI 42 GCGGG CCCGC(4/6)
FokI 35 GGATG GGATG(9/13)
HapII 1091 CCGG C^CGG
HpaII 1091 CCGG C^CGG
Ksp632I 321 CTCTTC CTCTTC(1/4)
KspI 40 CCGCGG CCGC^GG
MboI 813 GATC ^GATC
MflI 812 GGATCC [GA]^GATC[CT]
MluNI 740 TGGCCA TGG^CCA
MscI 740 TGGCCA TGG^CCA
MseI 605 TTAA T^TAA
MslI 232 CATGGCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 1091 CCGG C^CGG
NdeII 813 GATC ^GATC
PmaCI 1132 CACGTG CAC^GTG
PmlI 1132 CACGTG CAC^GTG
Psp1406I 376 AACGTT AA^CGTT
RcaI 89 TCATGA T^CATGA
SacII 40 CCGCGG CCGC^GG
Sau3AI 813 GATC ^GATC
SfcI 1014 CTATAG C^T[GA][CT]AG
StuI 715 AGGCCT AGG^CCT
StyI 198 CCTTGG C^C[AT][AT]GG
TfiI 936 GAATC G^A[AT]TC
Tru9I 605 TTAA T^TAA
XhoII 812 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcsI 274 AAATTC [GA]^AATT[CT]
755 AAATTT
AlwI 813 GATCC GGATC(4/5)
812 GGATC
ApaI 28 GGGCCC GGGCC^C
674 GGGCCC
ApoI 274 AAATTC [GA]^AATT[CT]
755 AAATTT
Asp700I 140 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
422 GAAATGTTTC
AvaI 632 CCCGAG C^[CT]CG[GA]G
888 CTCGGG
BbvI 944 GCAGC GCAGC(8/12)
75 GCTGC
BsiYI 130 CCTACCCAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
702 CCAGTCTGAGG
BslI 130 CCTACCCAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
702 CCAGTCTGAGG
BsmAI 391 GAGAC GTCTC(1/5)
225 GTCTC
BsmFI 1030 GGGAC GGGAC(10/14)
1087 GGGAC
BsoBI 632 CCCGAG C^[CT]CG[GA]G
888 CTCGGG
DraIII 217 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
1132 CACGTGGTG
MaeII 377 ACGT A^CGT
1133 ACGT
NlaIII 90 CATG CATG^
232 CATG
PleI 555 GACTC GAGTC(4/5)
93 GAGTC
PpuMI 1023 AGGACCT [GA]G^G[AT]CC[CT]
1030 GGGACCT
PvuII 591 CAGCTG CAG^CTG
945 CAGCTG
TseI 75 GCTGC G^C[AT]GC
944 GCAGC
XmnI 140 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
422 GAAATGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AccII AciI AfaI AluI AvaII
BanII BfaI BmyI BpmI BsaJI
Bsp1286I BsrI BssHII BssKI BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DraII EaeI EcoO109I EcoRII Fnu4HI
HaeIII HhaI HinP1I HinfI HphI
ItaI MaeI MaeIII MboII MnlI
MspA1I MvaI MvnI MwoI NlaIV
RsaI Sau96I ScrFI SfaNI Tsp45I
Tsp509I TspRI