################################## # NO CUT ENZYMES ( 113 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AgeI AhdI Alw44I ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BamHI BbeI BbrPI BciVI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I CelII Cfr10I ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco52I EcoO65I EcoRV EcoT22I FseI FspI Hin1I HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccII 1528 CGCG CG^CG AflII 1885 CTTAAG C^TTAAG AflIII 1400 ACATGT A^C[GA][CT]GT AocI 637 CCTGAGG CC^T[ACGT]AGG Aor51HI 743 AGCGCT AGC^GCT AspHI 544 GTGCTC G[AT]GC[AT]^C AspI 511 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 444 TGGCCA TGG^CCA BanI 97 GGCACC G^G[CT][GA]CC BcgI 536 CGAATCTTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 956 TGATCA T^GATCA BfaI 1276 CTAG C^TAG BfrI 1885 CTTAAG C^TTAAG BsiHKAI 544 GTGCTC G[AT]GC[AT]^C BspLU11I 1400 ACATGT A^CATGT BssSI 2174 CACGAG CACGAG(-5/-1) BstUI 1528 CGCG CG^CG Bsu36I 637 CCTGAGG CC^T[ACGT]AGG DrdI 1495 GACCAATGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 444 TGGCCA [CT]^GGCC[GA] Eco47III 743 AGCGCT AGC^GCT Eco81I 637 CCTGAGG CC^T[ACGT]AGG EcoNI 2210 CCTGGCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 1594 GAATTC G^AATTC FbaI 956 TGATCA T^GATCA HgaI 384 GCGTC GACGC(5/10) HincII 1165 GTTGAC GT[CT]^[GA]AC HindII 1165 GTTGAC GT[CT]^[GA]AC MaeI 1276 CTAG C^TAG MluNI 444 TGGCCA TGG^CCA MscI 444 TGGCCA TGG^CCA MvnI 1528 CGCG CG^CG NcoI 114 CCATGG C^CATGG NdeI 1351 CATATG CA^TATG PflFI 511 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 1956 GACAGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 1315 CTGCAG CTGCA^G SexAI 1337 ACCAGGT A^CC[AT]GGT SfaNI 178 GCATC GCATC(5/9) SmaI 938 CCCGGG CCC^GGG Tth111I 511 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 1382 CCATCTGCGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 938 CCCGGG C^CCGGG XmaI 938 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AcsI 1594 GAATTC [GA]^AATT[CT] 2241 GAATTT AfaI 39 GTAC GT^AC 165 GTAC AlwI 1182 GATCC GGATC(4/5) 848 GGATC ApoI 1594 GAATTC [GA]^AATT[CT] 2241 GAATTT AvaI 90 CTCGGG C^[CT]CG[GA]G 938 CCCGGG BbsI 705 GAAGAC GAAGAC(2/6) 555 GTCTTC BbvCI 108 CCTCAGC CCTCAGC(-5/-2) 2214 GCTGAGG BpuAI 705 GAAGAC GAAGAC(2/6) 555 GTCTTC BseRI 531 CTCCTC GAGGAG(10/8) 1066 CTCCTC BsgI 1333 CTGCAC GTGCAG(16/14) 1749 CTGCAC BsoBI 90 CTCGGG C^[CT]CG[GA]G 938 CCCGGG BspHI 990 TCATGA T^CATGA 1043 TCATGA BspMI 767 ACCTGC ACCTGC(4/8) 1471 ACCTGC BsrBI 562 GAGCGG CCGCTC(-3/-3) 2056 GAGCGG BsrDI 1519 CATTGC GCAATG(2/0) 332 GCAATG BstYI 848 GGATCT [GA]^GATC[CT] 1181 AGATCC EarI 2194 CTCTTC CTCTTC(1/4) 1200 GAAGAG EcoT14I 114 CCATGG C^C[AT][AT]GG 2115 CCTTGG HaeII 743 AGCGCT [GA]GCGC^[CT] 826 AGCGCC Ksp632I 2194 CTCTTC CTCTTC(1/4) 1200 GAAGAG MaeII 80 ACGT A^CGT 644 ACGT MaeIII 1444 GTCAC ^GT[ACGT]AC 1975 GTGAC MflI 848 GGATCT [GA]^GATC[CT] 1181 AGATCC PvuII 422 CAGCTG CAG^CTG 1160 CAGCTG RcaI 990 TCATGA T^CATGA 1043 TCATGA RsaI 39 GTAC GT^AC 165 GTAC SfcI 1315 CTGCAG C^T[GA][CT]AG 1797 CTGTAG SphI 1302 GCATGC GCATG^C 2201 GCATGC StyI 114 CCATGG C^C[AT][AT]GG 2115 CCTTGG TaqI 344 TCGA T^CGA 535 TCGA Tsp45I 1444 GTCAC ^GT[GC]AC 1975 GTGAC Tsp509I 1595 AATT ^AATT 2242 AATT TthHB8I 344 TCGA T^CGA 535 TCGA XhoII 848 GGATCT [GA]^GATC[CT] 1181 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 67 enzymes) # ######################################### AciI AluI AlwNI ApaI AvaII BanII BbvI BcnI BglI BmyI BpmI BsaI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstXI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MboI MboII MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI Sau3AI Sau96I ScrFI SmlI TfiI Tru9I TseI TspRI