################################## # NO CUT ENZYMES ( 85 enzymes) # ################################## AatII AccIII AclI AflII AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I AspEI AviII AvrII BbrPI BcgI BfrI BlnI BlpI Bpu1102I BseAI BsiEI BsiWI BspDI BspEI BspHI BsrBI BssHII Bst1107I BstBI BstEII BstPI BstZ17I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I FseI FspI HgaI HpaI KspI MluI MroI NaeI NdeI NgoMIV NotI NruI NspV PacI PaeR7I PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI SexAI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspI SwaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccI 1628 GTAGAC GT^[AC][GT]AC AccII 2125 CGCG CG^CG AgeI 660 ACCGGT A^CCGGT AlwNI 2609 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 1720 CCTCAGG CC^T[ACGT]AGG AspI 141 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 2889 TGGCCA TGG^CCA BamHI 2253 GGATCC G^GATCC BbsI 1114 GAAGAC GAAGAC(2/6) BclI 1287 TGATCA T^GATCA BglII 1095 AGATCT A^GATCT BpuAI 1114 GAAGAC GAAGAC(2/6) BsaAI 1488 CACGTA [CT]AC^GT[GA] BsaBI 1267 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 660 ACCGGT [AT]^CCGG[AT] BsmBI 139 GAGACG CGTCTC(1/5) BsmI 1966 GAATGC GAATGC(1/-1) Bsp1407I 1910 TGTACA T^GTACA BspMI 3258 ACCTGC ACCTGC(4/8) BsrFI 660 ACCGGT [GA]^CCGG[CT] BsrGI 1910 TGTACA T^GTACA BssSI 2234 CACGAG CACGAG(-5/-1) BstUI 2125 CGCG CG^CG Bsu36I 1720 CCTCAGG CC^T[ACGT]AGG Cfr10I 660 ACCGGT [GA]^CCGG[CT] Eco81I 1720 CCTCAGG CC^T[ACGT]AGG EcoRI 2016 GAATTC G^AATTC EcoRV 556 GATATC GAT^ATC FbaI 1287 TGATCA T^GATCA MaeII 1489 ACGT A^CGT MamI 1267 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 486 CAATTG C^AATTG MluNI 2889 TGGCCA TGG^CCA MscI 2889 TGGCCA TGG^CCA MunI 486 CAATTG C^AATTG MvnI 2125 CGCG CG^CG NheI 1045 GCTAGC G^CTAGC PflFI 141 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 119 CCAAGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 660 ACCGGT A^CCGGT SmlI 2011 CTTGAG C^T[CT][GA]AG SphI 3155 GCATGC GCATG^C SspBI 1910 TGTACA T^GTACA StuI 78 AGGCCT AGG^CCT Tth111I 141 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 119 CCAAGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### Acc65I 2184 GGTACC G^GTACC 3192 GGTACC AcyI 43 GGCGCC G[GA]^CG[CT]C 346 GGCGCC ApaI 108 GGGCCC GGGCC^C 2296 GGGCCC Asp718I 2184 GGTACC G^GTACC 3192 GGTACC AspHI 1406 GTGCTC G[AT]GC[AT]^C 2858 GAGCAC BbeI 43 GGCGCC GGCGC^C 346 GGCGCC BbvCI 2624 CCTCAGC CCTCAGC(-5/-2) 3302 CCTCAGC BciVI 2478 GGATAC GTATCC(6/5) 3219 GTATCC BglI 4 GCCGCAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2623 GCCTCAGCGGC BsaHI 43 GGCGCC G[GA]^CG[CT]C 346 GGCGCC BsgI 244 GTGCAG GTGCAG(16/14) 478 GTGCAG BsiHKAI 1406 GTGCTC G[AT]GC[AT]^C 2858 GAGCAC BspLU11I 1941 ACATGT A^CATGT 2530 ACATGT BsrDI 2283 CATTGC GCAATG(2/0) 1639 GCAATG EcoNI 1195 CCTGTTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1276 CCTGCCAAAGG EcoT22I 1361 ATGCAT ATGCA^T 1968 ATGCAT HaeII 43 GGCGCC [GA]GCGC^[CT] 346 GGCGCC Hin1I 43 GGCGCC G[GA]^CG[CT]C 346 GGCGCC HincII 1303 GTTGAC GT[CT]^[GA]AC 1615 GTTGAC HindII 1303 GTTGAC GT[CT]^[GA]AC 1615 GTTGAC HindIII 602 AAGCTT A^AGCTT 2314 AAGCTT KasI 43 GGCGCC G^GCGCC 346 GGCGCC KpnI 2184 GGTACC GGTAC^C 3192 GGTACC NarI 43 GGCGCC GG^CGCC 346 GGCGCC NcoI 784 CCATGG C^CATGG 1634 CCATGG NsiI 1361 ATGCAT ATGCA^T 1968 ATGCAT ScaI 1149 AGTACT AGT^ACT 2089 AGTACT SfoI 43 GGCGCC GGC^GCC 346 GGCGCC SmaI 3073 CCCGGG CCC^GGG 3188 CCCGGG XbaI 1221 TCTAGA T^CTAGA 2781 TCTAGA XmaCI 3073 CCCGGG C^CCGGG 3188 CCCGGG XmaI 3073 CCCGGG C^CCGGG 3188 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 95 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI ApoI AvaI AvaII BanI BanII BbvI BcnI BfaI BmyI BpmI BsaI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII