(Linear) (Six-base) MAPSORT of: KIEE0399.seq Check: 6048 from: 1 to: 3315 KIEE0399.orf With 182 enzymes: SgfI * July 15, 2010 18:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 3267 3315 Size: 3267 48 AccI GT'mk_AC Cuts at: 0 1629 3315 Size: 1629 1686 Acc65I G'GTAC_C Cuts at: 0 2184 3192 3315 Size: 2184 1008 123 Fragments arranged by size: 2184 1008 123 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 238 553 2046 2348 2525 2860 3315 Size: 238 315 1493 302 177 335 455 Fragments arranged by size: 1493 455 335 315 302 238 177 AflIII A'CryG_T Cuts at: 0 1487 1941 2530 3315 Size: 1487 454 589 785 Fragments arranged by size: 1487 785 589 454 AgeI A'CCGG_T Cuts at: 0 660 3315 Size: 660 2655 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2669 3315 Size: 2669 646 AlwNI CAG_nnn'CTG Cuts at: 0 2614 3315 Size: 2614 701 ApaI G_GGCC'C Cuts at: 0 112 2300 3315 Size: 112 2188 1015 Fragments arranged by size: 2188 1015 112 ApoI r'AATT_y Cuts at: 0 1473 1749 2016 2579 3315 Size: 1473 276 267 563 736 Fragments arranged by size: 1473 736 563 276 267 AvaI C'yCGr_G Cuts at: 0 54 2168 3073 3188 3315 Size: 54 2114 905 115 127 Fragments arranged by size: 2114 905 127 115 54 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 713 746 1112 1145 3315 Size: 713 33 366 33 2170 Fragments arranged by size: 2170 713 366 33 33 BamHI G'GATC_C Cuts at: 0 2253 3315 Size: 2253 1062 BanI G'GyrC_C Cuts at: 0 43 346 2184 2957 3192 3315 Size: 43 303 1838 773 235 123 Fragments arranged by size: 1838 773 303 235 123 43 BanII G_rGCy'C Cuts at: 0 55 112 136 2300 3315 Size: 55 57 24 2164 1015 Fragments arranged by size: 2164 1015 57 55 24 BbeI G_GCGC'C Cuts at: 0 47 350 3315 Size: 47 303 2965 Fragments arranged by size: 2965 303 47 BbsI GAAGACnn'nnnn_ Cuts at: 0 1121 3315 Size: 1121 2194 BbvCI CC'TCA_GC Cuts at: 0 2625 3303 3315 Size: 2625 678 12 Fragments arranged by size: 2625 678 12 BciVI GTATCCnnnnn_n' Cuts at: 0 2472 3230 3315 Size: 2472 758 85 Fragments arranged by size: 2472 758 85 BclI T'GATC_A Cuts at: 0 1287 3315 Size: 1287 2028 BfrBI ATG'CAT Cuts at: 0 1363 1970 3315 Size: 1363 607 1345 Fragments arranged by size: 1363 1345 607 BglI GCCn_nnn'nGGC Cuts at: 0 10 2629 3315 Size: 10 2619 686 Fragments arranged by size: 2619 686 10 BglII A'GATC_T Cuts at: 0 1095 3315 Size: 1095 2220 Bme1580I G_kGCm'C Cuts at: 0 112 2300 2960 3315 Size: 112 2188 660 355 Fragments arranged by size: 2188 660 355 112 BmrI ACTGGGnnnn_n' Cuts at: 0 2564 3315 Size: 2564 751 BmtI G_CTAG'C Cuts at: 0 1049 3315 Size: 1049 2266 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 362 1433 1568 1915 1924 2290 3315 Size: 362 1071 135 347 9 366 1025 Fragments arranged by size: 1071 1025 366 362 347 135 9 Bpu10I CC'TnA_GC Cuts at: 0 2625 3303 3315 Size: 2625 678 12 Fragments arranged by size: 2625 678 12 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2032 3315 Size: 2032 1283 BsaI GGTCTCn'nnnn_ Cuts at: 0 651 1804 1873 3315 Size: 651 1153 69 1442 Fragments arranged by size: 1442 1153 651 69 BsaAI yAC'GTr Cuts at: 0 1490 3315 Size: 1490 1825 BsaBI GATnn'nnATC Cuts at: 0 1271 3315 Size: 1271 2044 BsaHI Gr'CG_yC Cuts at: 0 44 347 3315 Size: 44 303 2968 Fragments arranged by size: 2968 303 44 BsaWI w'CCGG_w Cuts at: 0 660 3315 Size: 660 2655 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 130 160 1196 1226 1801 1831 3315 Size: 130 30 1036 30 575 30 1484 Fragments arranged by size: 1484 1036 575 130 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 859 1640 1649 2173 2194 3315 Size: 859 781 9 524 21 1121 Fragments arranged by size: 1121 859 781 524 21 9 BseYI C'CCAG_C Cuts at: 0 149 174 232 3035 3315 Size: 149 25 58 2803 280 Fragments arranged by size: 2803 280 149 58 25 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 265 499 3315 Size: 265 234 2816 Fragments arranged by size: 2816 265 234 BsiHKAI G_wGCw'C Cuts at: 0 1410 2862 3315 Size: 1410 1452 453 Fragments arranged by size: 1452 1410 453 BsmI GAATG_Cn' Cuts at: 0 1972 3315 Size: 1972 1343 BsmBI CGTCTCn'nnnn_ Cuts at: 0 133 3315 Size: 133 3182 Bsp1286I G_dGCh'C Cuts at: 0 55 112 136 1410 2300 2862 2960 3315 Size: 55 57 24 1274 890 562 98 355 Fragments arranged by size: 1274 890 562 355 98 57 55 24 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 3267 3315 Size: 3267 48 BsrDI GCAATG_nn' Cuts at: 0 1646 2282 3315 Size: 1646 636 1033 Fragments arranged by size: 1646 1033 636 BsrFI r'CCGG_y Cuts at: 0 660 3315 Size: 660 2655 BsrGI T'GTAC_A Cuts at: 0 1910 3315 Size: 1910 1405 BssSI C'ACGA_G Cuts at: 0 2234 3315 Size: 2234 1081 BstAPI GCAn_nnn'nTGC Cuts at: 0 2137 2614 2802 3084 3315 Size: 2137 477 188 282 231 Fragments arranged by size: 2137 477 282 231 188 BstXI CCAn_nnnn'nTGG Cuts at: 0 1110 1641 2649 3130 3315 Size: 1110 531 1008 481 185 Fragments arranged by size: 1110 1008 531 481 185 BstYI r'GATC_y Cuts at: 0 762 1095 1218 1272 2253 3315 Size: 762 333 123 54 981 1062 Fragments arranged by size: 1062 981 762 333 123 54 Bsu36I CC'TnA_GG Cuts at: 0 1721 3315 Size: 1721 1594 BtgI C'CryG_G Cuts at: 0 47 784 1634 3315 Size: 47 737 850 1681 Fragments arranged by size: 1681 850 737 47 BtsI GCAGTG_nn' Cuts at: 0 1523 1597 2663 3315 Size: 1523 74 1066 652 Fragments arranged by size: 1523 1066 652 74 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 2177 2212 3315 Size: 2177 35 1103 Fragments arranged by size: 2177 1103 35 EaeI y'GGCC_r Cuts at: 0 2 113 2889 3315 Size: 2 111 2776 426 Fragments arranged by size: 2776 426 111 2 EarI CTCTTCn'nnn_ Cuts at: 0 908 933 1018 1415 1854 2324 2501 2738 Size: 908 25 85 397 439 470 177 237 Cuts at: 2738 3315 Size: 577 Fragments arranged by size: 908 577 470 439 397 237 177 85 25 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 417 3266 3315 Size: 417 2849 49 Fragments arranged by size: 2849 417 49 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 238 362 553 1433 1568 1915 1924 2046 Size: 238 124 191 880 135 347 9 122 Cuts at: 2046 2290 2348 2525 2860 3315 Size: 244 58 177 335 455 Fragments arranged by size: 880 455 347 335 244 238 191 177 135 124 122 58 9 EcoNI CCTnn'n_nnAGG Cuts at: 0 1199 1280 3315 Size: 1199 81 2035 Fragments arranged by size: 2035 1199 81 EcoO109I rG'GnC_Cy Cuts at: 0 108 1645 1899 2001 2296 2926 3184 3270 Size: 108 1537 254 102 295 630 258 86 Cuts at: 3270 3315 Size: 45 Fragments arranged by size: 1537 630 295 258 254 108 102 86 45 EcoRI G'AATT_C Cuts at: 0 2016 3315 Size: 2016 1299 EcoRV GAT'ATC Cuts at: 0 558 3315 Size: 558 2757 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1066 1168 3315 Size: 1066 102 2147 Fragments arranged by size: 2147 1066 102 HaeII r_GCGC'y Cuts at: 0 47 350 3315 Size: 47 303 2965 Fragments arranged by size: 2965 303 47 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 130 162 491 523 580 612 873 905 Size: 130 32 329 32 57 32 261 32 Cuts at: 905 1672 1704 1801 1833 2470 2502 2961 2993 Size: 767 32 97 32 637 32 459 32 Cuts at: 2993 3315 Size: 322 Fragments arranged by size: 767 637 459 329 322 261 130 97 57 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 1305 1617 3315 Size: 1305 312 1698 Fragments arranged by size: 1698 1305 312 HindIII A'AGCT_T Cuts at: 0 602 2314 3315 Size: 602 1712 1001 Fragments arranged by size: 1712 1001 602 KasI G'GCGC_C Cuts at: 0 43 346 3315 Size: 43 303 2969 Fragments arranged by size: 2969 303 43 KpnI G_GTAC'C Cuts at: 0 2188 3196 3315 Size: 2188 1008 119 Fragments arranged by size: 2188 1008 119 MfeI C'AATT_G Cuts at: 0 486 3315 Size: 486 2829 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1024 1513 1916 1926 3315 Size: 1024 489 403 10 1389 Fragments arranged by size: 1389 1024 489 403 10 MscI TGG'CCA Cuts at: 0 2891 3315 Size: 2891 424 MslI CAynn'nnrTG Cuts at: 0 206 737 1639 1667 3315 Size: 206 531 902 28 1648 Fragments arranged by size: 1648 902 531 206 28 MspA1I CmG'CkG Cuts at: 0 10 37 153 894 2279 2614 2629 2838 Size: 10 27 116 741 1385 335 15 209 Cuts at: 2838 3307 3315 Size: 469 8 Fragments arranged by size: 1385 741 469 335 209 116 27 15 10 8 NarI GG'CG_CC Cuts at: 0 44 347 3315 Size: 44 303 2968 Fragments arranged by size: 2968 303 44 NcoI C'CATG_G Cuts at: 0 784 1634 3315 Size: 784 850 1681 Fragments arranged by size: 1681 850 784 NheI G'CTAG_C Cuts at: 0 1045 3315 Size: 1045 2270 NsiI A_TGCA'T Cuts at: 0 1365 1972 3315 Size: 1365 607 1343 Fragments arranged by size: 1365 1343 607 NspI r_CATG'y Cuts at: 0 286 1945 2534 3087 3159 3219 3315 Size: 286 1659 589 553 72 60 96 Fragments arranged by size: 1659 589 553 286 96 72 60 PciI A'CATG_T Cuts at: 0 1941 2530 3315 Size: 1941 589 785 Fragments arranged by size: 1941 785 589 PflMI CCAn_nnn'nTGG Cuts at: 0 125 3315 Size: 125 3190 PfoI T'CCnGG_A Cuts at: 0 260 1892 2174 3315 Size: 260 1632 282 1141 Fragments arranged by size: 1632 1141 282 260 PpuMI rG'GwC_Cy Cuts at: 0 1645 2001 2926 3184 3270 3315 Size: 1645 356 925 258 86 45 Fragments arranged by size: 1645 925 356 258 86 45 PsiI TTA'TAA Cuts at: 0 1379 3315 Size: 1379 1936 PspOMI G'GGCC_C Cuts at: 0 108 2296 3315 Size: 108 2188 1019 Fragments arranged by size: 2188 1019 108 PstI C_TGCA'G Cuts at: 0 2142 2658 3264 3315 Size: 2142 516 606 51 Fragments arranged by size: 2142 606 516 51 PvuII CAG'CTG Cuts at: 0 153 2279 2614 2838 3307 3315 Size: 153 2126 335 224 469 8 Fragments arranged by size: 2126 469 335 224 153 8 SanDI GG'GwC_CC Cuts at: 0 3270 3315 Size: 3270 45 SapI GCTCTTCn'nnn_ Cuts at: 0 908 1415 2738 3315 Size: 908 507 1323 577 Fragments arranged by size: 1323 908 577 507 ScaI AGT'ACT Cuts at: 0 1151 2091 3315 Size: 1151 940 1224 Fragments arranged by size: 1224 1151 940 SfcI C'TryA_G Cuts at: 0 415 1211 2138 2654 3260 3315 Size: 415 796 927 516 606 55 Fragments arranged by size: 927 796 606 516 415 55 SfoI GGC'GCC Cuts at: 0 45 348 3315 Size: 45 303 2967 Fragments arranged by size: 2967 303 45 SmaI CCC'GGG Cuts at: 0 3075 3190 3315 Size: 3075 115 125 Fragments arranged by size: 3075 125 115 SmlI C'TyrA_G Cuts at: 0 2011 3315 Size: 2011 1304 SphI G_CATG'C Cuts at: 0 3159 3315 Size: 3159 156 SrfI GCCC'GGGC Cuts at: 0 3075 3315 Size: 3075 240 StuI AGG'CCT Cuts at: 0 80 3315 Size: 80 3235 StyI C'CwwG_G Cuts at: 0 784 1103 1308 1634 2031 3315 Size: 784 319 205 326 397 1284 Fragments arranged by size: 1284 784 397 326 319 205 TatI w'GTAC_w Cuts at: 0 1149 1910 2089 2220 3315 Size: 1149 761 179 131 1095 Fragments arranged by size: 1149 1095 761 179 131 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1011 1043 3315 Size: 1011 32 2272 Fragments arranged by size: 2272 1011 32 Tth111I GACn'n_nGTC Cuts at: 0 144 3315 Size: 144 3171 XbaI T'CTAG_A Cuts at: 0 1221 2781 3315 Size: 1221 1560 534 Fragments arranged by size: 1560 1221 534 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 225 782 2265 3315 Size: 225 557 1483 1050 Fragments arranged by size: 1483 1050 557 225 XmaI C'CCGG_G Cuts at: 0 3073 3188 3315 Size: 3073 115 127 Fragments arranged by size: 3073 127 115 Enzymes that do cut: AarI AccI Acc65I AcuI AflIII AgeI AlfI AlwNI ApaI ApoI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglI BglII Bme1580I BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrDI BsrFI BsrGI BssSI BstAPI BstXI BstYI Bsu36I BtgI BtsI CspCI EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HindIII KasI KpnI MfeI MmeI MscI MslI MspA1I NarI NcoI NheI NsiI NspI PciI PflMI PfoI PpuMI PsiI PspOMI PstI PvuII SanDI SapI ScaI SfcI SfoI SmaI SmlI SphI SrfI StuI StyI TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AatII AclI AfeI AflII AhdI AleI AloI ApaLI AscI AseI AsiSI AvrII BcgI BlpI BmgBI BplI BsiEI BsiWI BspEI BspHI BsrBI BssHII BstBI BstEII BstZ17I BtgZI ClaI DraI DraIII DrdI EagI EcoICRI FseI FspI FspAI HpaI MluI NaeI NdeI NgoMIV NotI NruI PacI PasI PmeI PmlI PpiI PshAI PspXI PsrI PvuI RsrII SacI SacII SalI SbfI SexAI SfiI SgfI SgrAI SnaBI SpeI SspI SwaI TaqII XhoI XmnI ZraI