Restriction map for
pFN21AE0417
(Circular) (Six-base) MAPSORT of: pFN21AE0417.seq Check: 3626 from: 1 to: 5467
pFN21AE0417 5467 bp
With 182 enzymes: SgfI *
October 20, 2009 10:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3277 278
Size: 53 83 186 936 1741 2468
Fragments arranged by size:
2468 1741 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2810 1937
Size: 873 4594
Acc65I G'GTAC_C
Cuts at: 2788 2788
Size: 5467
AclI AA'CG_TT
Cuts at: 1563 3594 3967 1563
Size: 2031 373 3063
Fragments arranged by size:
3063 2031 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2139 2360 3530 4914 5182 1632
Size: 507 221 1170 1384 268 1917
Fragments arranged by size:
1917 1384 1170 507 268 221
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5270
AflIII A'CryG_T
Cuts at: 1203 4366 5343 1203
Size: 3163 977 1327
Fragments arranged by size:
3163 1327 977
AgeI A'CCGG_T
Cuts at: 4298 4298
Size: 5467
AhdI GACnn_n'nnGTC
Cuts at: 2677 4196 2677
Size: 1519 3948
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5467
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5467
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2429 2461 2429
Size: 32 5435
AlwNI CAG_nnn'CTG
Cuts at: 2471 3210 4782 2471
Size: 739 1572 3156
Fragments arranged by size:
3156 1572 739
ApaLI G'TGCA_C
Cuts at: 3524 4680 3524
Size: 1156 4311
ApoI r'AATT_y
Cuts at: 1493 2225 2373 2776 3002 3154 5263 1493
Size: 732 148 403 226 152 2109 1697
Fragments arranged by size:
2109 1697 732 403 226 152 148
AseI AT'TA_AT
Cuts at: 160 1032 4021 160
Size: 872 2989 1606
Fragments arranged by size:
2989 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5467
AvaI C'yCGr_G
Cuts at: 1870 1943 2756 2792 1870
Size: 73 813 36 4545
Fragments arranged by size:
4545 813 73 36
BamHI G'GATC_C
Cuts at: 2797 3164 2797
Size: 367 5100
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2651 2699 2788 4243 618
Size: 325 217 627 864 48 89 1455 1842
Fragments arranged by size:
1842 1455 864 627 325 217 89 48
BanII G_rGCy'C
Cuts at: 1391 2489 2556 2786 1391
Size: 1098 67 230 4072
Fragments arranged by size:
4072 1098 230 67
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4496
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2554 2588 3658 3692 1903
Size: 34 617 34 1070 34 3678
Fragments arranged by size:
3678 1070 617 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3359 4569 3359
Size: 1210 4257
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5467
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4078 136
Size: 107 122 71 631 605 2406 1525
Fragments arranged by size:
2406 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5462 5462
Size: 5467
BlpI GC'TnA_GC
Cuts at: 2884 2884
Size: 5467
Bme1580I G_kGCm'C
Cuts at: 1790 3528 4684 1790
Size: 1738 1156 2573
Fragments arranged by size:
2573 1738 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5467
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3198 4156 442
Size: 416 53 83 399 24 1781 958 1753
Fragments arranged by size:
1781 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5467
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2701 2701
Size: 5467
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4127 1239
Size: 2888 2579
Bpu10I CC'TnA_GC
Cuts at: 2529 2529
Size: 5467
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2345 2364 3592 4457 4755 2345
Size: 19 1228 865 298 3057
Fragments arranged by size:
3057 1228 865 298 19
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2255 4130 882
Size: 617 756 1875 2219
Fragments arranged by size:
2219 1875 756 617
BsaAI yAC'GTr
Cuts at: 493 1204 3258 493
Size: 711 2054 2702
Fragments arranged by size:
2702 2054 711
BsaBI GATnn'nnATC
Cuts at: 5461 5461
Size: 5467
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3274 3656 4291 275
Size: 53 83 186 936 1741 382 635 1451
Fragments arranged by size:
1741 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2521 2714 3900 4298 4572 4719 5130 2521
Size: 193 1186 398 274 147 411 2858
Fragments arranged by size:
2858 1186 411 398 274 193 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2429 2459 1178
Size: 30 165 30 1026 30 4186
Fragments arranged by size:
4186 1026 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2156 2719 1186
Size: 970 563 3934
Fragments arranged by size:
3934 970 563
BseYI C'CCAG_C
Cuts at: 4670 5166 4670
Size: 496 4971
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4894
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2917 3678 4706 1540
Size: 275 186 916 761 1028 2301
Fragments arranged by size:
2301 1028 916 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2489 3528 3613 4684 2489
Size: 1039 85 1071 3272
Fragments arranged by size:
3272 1071 1039 85
BsmI GAATG_Cn'
Cuts at: 2978 3071 2978
Size: 93 5374
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2489 2556 2786 3528 3613 4684 1391
Size: 399 699 67 230 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 699 399 230 85 67
BspEI T'CCGG_A
Cuts at: 5130 5130
Size: 5467
BspHI T'CATG_A
Cuts at: 3356 5086 3356
Size: 1730 3737
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2822 844
Size: 1978 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3354 1394
Size: 429 1531 3507
Fragments arranged by size:
3507 1531 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3962 4136 65
Size: 1183 2714 174 1396
Fragments arranged by size:
2714 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4111 4298 1700
Size: 110 2301 187 2869
Fragments arranged by size:
2869 2301 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5467
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5467
BssSI C'ACGA_G
Cuts at: 3527 4539 3527
Size: 1012 4455
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5467
BstBI TT'CG_AA
Cuts at: 2110 4271 2110
Size: 2161 3306
BstEII G'GTnAC_C
Cuts at: 1184 2024 1184
Size: 840 4627
BstYI r'GATC_y
Cuts at: 1972 2122 2797 3164 3549 3566 5018 5104 5116
Size: 150 675 367 385 17 1452 86 12
Cuts at: 5116 5462 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 675 385 367 346 150 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3976
Fragments arranged by size:
3976 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4728
Fragments arranged by size:
4728 683 56
BtsI GCAGTG_nn'
Cuts at: 2605 2983 3777 3797 2605
Size: 378 794 20 4275
Fragments arranged by size:
4275 794 378 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5467
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5432
DraI TTT'AAA
Cuts at: 2771 2771
Size: 5467
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4474 809
Size: 507 3158 1802
Fragments arranged by size:
3158 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2914 3803 5432
Size: 54 1425 50 275 18 1084 889 1629
Cuts at: 5432 8
Size: 43
Fragments arranged by size:
1629 1425 1084 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2914 1537
Size: 275 1102 4090
Fragments arranged by size:
4090 1102 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2311 3397 1353
Size: 958 1086 3423
Fragments arranged by size:
3423 1086 958
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4054 4428 4574 387
Size: 1064 138 2465 374 146 1280
Fragments arranged by size:
2465 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2487 2487
Size: 5467
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2139 2360 3530 4127 4914 5182 1239
Size: 393 507 221 1170 597 787 268 1524
Fragments arranged by size:
1524 1170 787 597 507 393 268 221
EcoNI CCTnn'n_nnAGG
Cuts at: 2276 2363 2276
Size: 87 5380
EcoO109I rG'GnC_Cy
Cuts at: 2761 2761
Size: 5467
EcoRI G'AATT_C
Cuts at: 2776 3154 2776
Size: 378 5089
FspI TGC'GCA
Cuts at: 3973 3973
Size: 5467
HaeII r_GCGC'y
Cuts at: 1678 4614 1678
Size: 2936 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2144 2176 2543 2575 2663
Size: 32 135 32 572 32 367 32 88
Cuts at: 2663 2695 4108 4140 4182 4214 1373
Size: 32 1413 32 42 32 2626
Fragments arranged by size:
2626 1413 572 367 135 88 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2811 3057 669
Size: 988 281 873 246 3079
Fragments arranged by size:
3079 988 873 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5467
HpaI GTT'AAC
Cuts at: 3057 3057
Size: 5467
KpnI G_GTAC'C
Cuts at: 2792 2792
Size: 5467
MfeI C'AATT_G
Cuts at: 3066 3066
Size: 5467
MluI A'CGCG_T
Cuts at: 5343 5343
Size: 5467
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2350 4581 4765 1305
Size: 1045 2231 184 2007
Fragments arranged by size:
2231 2007 1045 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3645
Fragments arranged by size:
3645 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3425 3784 3943 518
Size: 648 2259 359 159 2042
Fragments arranged by size:
2259 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2883 3560 4708 4953 1606
Size: 1277 677 1148 245 2120
Fragments arranged by size:
2120 1277 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3976
Fragments arranged by size:
3976 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5467
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5467
NotI GC'GGCC_GC
Cuts at: 2914 2914
Size: 5467
NspI r_CATG'y
Cuts at: 1132 1229 2825 4370 1132
Size: 97 1596 1545 2229
Fragments arranged by size:
2229 1596 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5467
PciI A'CATG_T
Cuts at: 4366 4366
Size: 5467
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5467
PmeI GTTT'AAAC
Cuts at: 2771 2771
Size: 5467
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2204 2236 2429 2461 3536 3568 5049 5081 2204
Size: 32 193 32 1075 32 1481 32 2590
Fragments arranged by size:
2590 1481 1075 193 32 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5467
PsiI TTA'TAA
Cuts at: 3037 3037
Size: 5467
PspXI vC'TCGA_Gb
Cuts at: 2756 2756
Size: 5467
PstI C_TGCA'G
Cuts at: 830 2623 2819 830
Size: 1793 196 3478
Fragments arranged by size:
3478 1793 196
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5467
SacI G_AGCT'C
Cuts at: 2489 2489
Size: 5467
SalI G'TCGA_C
Cuts at: 1936 2809 1936
Size: 873 4594
SbfI CC_TGCA'GG
Cuts at: 2819 2819
Size: 5467
ScaI AGT'ACT
Cuts at: 3715 5427 3715
Size: 1712 3755
SfcI C'TryA_G
Cuts at: 826 1045 2177 2424 2619 2815 3950 4631 4822
Size: 219 1132 247 195 196 1135 681 191
Cuts at: 4822 826
Size: 1471
Fragments arranged by size:
1471 1135 1132 681 247 219 196 195
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5467
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5467
SmaI CCC'GGG
Cuts at: 1872 2794 1872
Size: 922 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2360 2379 2756 3571 4472 4734
Size: 197 926 417 19 377 815 901 262
Cuts at: 4734 820
Size: 1553
Fragments arranged by size:
1553 926 901 815 417 377 262 197
19
SnaBI TAC'GTA
Cuts at: 493 3258 493
Size: 2765 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5467
SphI G_CATG'C
Cuts at: 1132 2825 1132
Size: 1693 3774
SspI AAT'ATT
Cuts at: 5 52 3391 5
Size: 47 3339 2081
Fragments arranged by size:
3339 2081 47
StuI AGG'CCT
Cuts at: 1517 2646 1517
Size: 1129 4338
StyI C'CwwG_G
Cuts at: 513 1065 2004 2906 513
Size: 552 939 902 3074
Fragments arranged by size:
3074 939 902 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2639 3494 3511 3664 3849 4294 325
Size: 1255 1059 855 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1059 855 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3713 5425 96
Size: 274 80 33 51 1443 1736 1712 138
Fragments arranged by size:
1736 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5427 5459 5427
Size: 32 5435
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5467
XbaI T'CTAG_A
Cuts at: 2803 2803
Size: 5467
XhoI C'TCGA_G
Cuts at: 1943 2756 1943
Size: 813 4654
XmaI C'CCGG_G
Cuts at: 1870 2792 1870
Size: 922 4545
XmnI GAAnn'nnTTC
Cuts at: 2441 3596 2441
Size: 1155 4312
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3275 276
Size: 53 83 186 936 1741 2468
Fragments arranged by size:
2468 1741 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI
PstI PvuI SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PsrI PvuII
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI