Restriction map for
pFN21AE2106
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# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII Acc65I AccI AclI AgeI
AhdI AscI AseI AsnI Asp718I
AspEI AspI AvrII BbrPI BbvCI
BcgI BciVI BlnI BlpI Bpu1102I
BsaBI BsiEI BsmI Bsp1407I BspDI
BspLU11I BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstXI BstZ17I CelII
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco52I EcoO65I
EcoRI EcoRV EcoT22I FseI HpaI
KpnI KspI MamI MluI NdeI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PvuI RsrII SacII SalI SapI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI SspBI SwaI Tth111I
Van91I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AflII 208 CTTAAG C^TTAAG
AflIII 35 ACGTGT A^C[GA][CT]GT
Alw44I 2630 GTGCAC G^TGCAC
AocI 153 CCTAAGG CC^T[ACGT]AGG
Aor51HI 1190 AGCGCT AGC^GCT
ApaLI 2630 GTGCAC G^TGCAC
AviII 1668 TGCGCA TGC^GCA
BbeI 1916 GGCGCC GGCGC^C
BfrI 208 CTTAAG C^TTAAG
BglI 1564 GCCAAGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1265 AGATCT A^GATCT
BsaAI 34 TACGTG [CT]AC^GT[GA]
BsaI 2090 GAGACC GGTCTC(1/5)
BsiWI 740 CGTACG C^GTACG
BsrBI 1711 GAGCGG CCGCTC(-3/-3)
BsrDI 374 GCAATG GCAATG(2/0)
Bsu36I 153 CCTAAGG CC^T[ACGT]AGG
Eco47III 1190 AGCGCT AGC^GCT
Eco81I 153 CCTAAGG CC^T[ACGT]AGG
FspI 1668 TGCGCA TGC^GCA
HincII 298 GTCAAC GT[CT]^[GA]AC
HindII 298 GTCAAC GT[CT]^[GA]AC
KasI 1916 GGCGCC G^GCGCC
MfeI 1587 CAATTG C^AATTG
MslI 2155 CACATTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 1587 CAATTG C^AATTG
NaeI 163 GCCGGC GCC^GGC
NarI 1916 GGCGCC GG^CGCC
NgoMIV 163 GCCGGC G^CCGGC
NheI 864 GCTAGC G^CTAGC
NspI 1896 GCATGC [GA]CATG^[CT]
PshAI 738 GACGTACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 1872 CCTGCAGG CCTGCA^GG
ScaI 2579 AGTACT AGT^ACT
SfoI 1916 GGCGCC GGC^GCC
SphI 1896 GCATGC GCATG^C
Sse8387I 1872 CCTGCAGG CCTGCA^GG
SspI 631 AATATT AAT^ATT
StuI 1226 AGGCCT AGG^CCT
XcmI 2201 CCATCAATGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 29 enzymes) #
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AccIII 1820 TCCGGA T^CCGGA
2504 TCCGGA
AcsI 1454 AAATTT [GA]^AATT[CT]
2377 GAATTT
AcyI 1293 GGCGTC G[GA]^CG[CT]C
1916 GGCGCC
ApaI 1948 GGGCCC GGGCC^C
2610 GGGCCC
ApoI 1454 AAATTT [GA]^AATT[CT]
2377 GAATTT
BalI 641 TGGCCA TGG^CCA
1727 TGGCCA
BamHI 1955 GGATCC G^GATCC
2507 GGATCC
BclI 1542 TGATCA T^GATCA
1661 TGATCA
BsaHI 1293 GGCGTC G[GA]^CG[CT]C
1916 GGCGCC
BseAI 1820 TCCGGA T^CCGGA
2504 TCCGGA
BsgI 22 GTGCAG GTGCAG(16/14)
1003 GTGCAG
BsmBI 744 CGTCTC CGTCTC(1/5)
2461 GAGACG
BspEI 1820 TCCGGA T^CCGGA
2504 TCCGGA
BspMI 86 ACCTGC ACCTGC(4/8)
2487 GCAGGT
BsrFI 163 GCCGGC [GA]^CCGG[CT]
578 ACCGGC
Cfr10I 163 GCCGGC [GA]^CCGG[CT]
578 ACCGGC
DraII 1948 GGGCCCT [GA]G^G[ACGT]CC[CT]
2111 AGGCCCT
EaeI 641 TGGCCA [CT]^GGCC[GA]
1727 TGGCCA
EarI 2652 CTCTTC CTCTTC(1/4)
1494 GAAGAG
EcoO109I 1948 GGGCCCT [GA]G^G[ACGT]CC[CT]
2111 AGGCCCT
FbaI 1542 TGATCA T^GATCA
1661 TGATCA
Hin1I 1293 GGCGTC G[GA]^CG[CT]C
1916 GGCGCC
HindIII 305 AAGCTT A^AGCTT
604 AAGCTT
Ksp632I 2652 CTCTTC CTCTTC(1/4)
1494 GAAGAG
MluNI 641 TGGCCA TGG^CCA
1727 TGGCCA
MroI 1820 TCCGGA T^CCGGA
2504 TCCGGA
MscI 641 TGGCCA TGG^CCA
1727 TGGCCA
PvuII 1018 CAGCTG CAG^CTG
2029 CAGCTG
SmlI 208 CTTAAG C^T[CT][GA]AG
1782 CTCAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI Asp700I AspHI AvaI AvaII
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaJI
BsaWI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspHI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DsaI EcoNI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV PleI PstI
RcaI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI