(Circular) (Six-base) MAPSORT of: pFN21AE2106.seq Check: 5823 from: 1 to: 7387 pFN21AE2106 7387 bp With 182 enzymes: SgfI * August 16, 2010 14:56 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5197 278 Size: 53 83 186 936 3661 2468 Fragments arranged by size: 3661 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4730 1937 Size: 2793 4594 Acc65I G'GTAC_C Cuts at: 4708 4708 Size: 7387 AclI AA'CG_TT Cuts at: 1563 5514 5887 1563 Size: 3951 373 3063 Fragments arranged by size: 3951 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2185 2382 2718 4058 5450 6834 7102 1632 Size: 553 197 336 1340 1392 1384 268 1917 Fragments arranged by size: 1917 1392 1384 1340 553 336 268 197 AfeI AGC'GCT Cuts at: 3197 3197 Size: 7387 AflII C'TTAA_G Cuts at: 820 1017 2213 820 Size: 197 1196 5994 Fragments arranged by size: 5994 1196 197 AflIII A'CryG_T Cuts at: 1203 2040 6286 7263 1203 Size: 837 4246 977 1327 Fragments arranged by size: 4246 1327 977 837 AgeI A'CCGG_T Cuts at: 6218 6218 Size: 7387 AhdI GACnn_n'nnGTC Cuts at: 6116 6116 Size: 7387 AleI CACnn'nnGTG Cuts at: 1166 4164 1166 Size: 2998 4389 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7387 AlwNI CAG_nnn'CTG Cuts at: 3136 3664 4231 4344 5130 6702 3136 Size: 528 567 113 786 1572 3821 Fragments arranged by size: 3821 1572 786 567 528 113 ApaI G_GGCC'C Cuts at: 3957 4619 3957 Size: 662 6725 ApaLI G'TGCA_C Cuts at: 4635 5444 6600 4635 Size: 809 1156 5422 Fragments arranged by size: 5422 1156 809 ApoI r'AATT_y Cuts at: 1493 3459 4382 4696 4922 5074 7183 1493 Size: 1966 923 314 226 152 2109 1697 Fragments arranged by size: 2109 1966 1697 923 314 226 152 AseI AT'TA_AT Cuts at: 160 1032 5941 160 Size: 872 4909 1606 Fragments arranged by size: 4909 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7387 AvaI C'yCGr_G Cuts at: 1870 1943 2930 3701 4612 4712 1870 Size: 73 987 771 911 100 4545 Fragments arranged by size: 4545 987 911 771 100 73 BamHI G'GATC_C Cuts at: 3960 4512 4717 5084 3960 Size: 552 205 367 6263 Fragments arranged by size: 6263 552 367 205 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2171 3122 3450 3921 4424 Size: 325 217 627 384 951 328 471 503 Cuts at: 4424 4708 6163 618 Size: 284 1455 1842 Fragments arranged by size: 1842 1455 951 627 503 471 384 328 325 284 217 BanII G_rGCy'C Cuts at: 1391 2022 3322 3408 3825 3957 3995 4015 4619 Size: 631 1300 86 417 132 38 20 604 Cuts at: 4619 4706 1391 Size: 87 4072 Fragments arranged by size: 4072 1300 631 604 417 132 87 86 38 20 BbeI G_GCGC'C Cuts at: 3925 3925 Size: 7387 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2135 2202 2706 2942 4026 928 Size: 971 236 67 504 236 1084 4289 Fragments arranged by size: 4289 1084 971 504 236 236 67 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5578 5612 1903 Size: 34 3641 34 3678 Fragments arranged by size: 3678 3641 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5279 6489 5279 Size: 1210 6177 BclI T'GATC_A Cuts at: 1548 3547 3666 1548 Size: 1999 119 5269 Fragments arranged by size: 5269 1999 119 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3575 5998 136 Size: 107 122 71 631 605 1903 2423 1525 Fragments arranged by size: 2423 1903 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3270 7382 3270 Size: 4112 3275 BlpI GC'TnA_GC Cuts at: 4804 4804 Size: 7387 Bme1580I G_kGCm'C Cuts at: 1790 2569 3861 3957 4619 4639 5448 6604 1790 Size: 779 1292 96 662 20 809 1156 2573 Fragments arranged by size: 2573 1292 1156 809 779 662 96 20 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7387 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3491 4576 5118 Size: 416 53 83 399 24 2074 1085 542 Cuts at: 5118 6076 442 Size: 958 1753 Fragments arranged by size: 2074 1753 1085 958 542 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2873 1056 Size: 1817 5570 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3597 3597 Size: 7387 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2936 2984 3047 3158 3212 3581 4109 6047 Size: 1697 48 63 111 54 369 528 1938 Cuts at: 6047 1239 Size: 2579 Fragments arranged by size: 2579 1938 1697 528 369 111 63 54 48 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3772 5512 6377 6675 3772 Size: 1740 865 298 4484 Fragments arranged by size: 4484 1740 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4089 6050 882 Size: 617 2590 1961 2219 Fragments arranged by size: 2590 2219 1961 617 BsaAI yAC'GTr Cuts at: 493 1204 2041 5178 493 Size: 711 837 3137 2702 Fragments arranged by size: 3137 2702 837 711 BsaBI GATnn'nnATC Cuts at: 7381 7381 Size: 7387 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3299 3922 5194 5576 Size: 53 83 186 936 1766 623 1272 382 Cuts at: 5576 6211 275 Size: 635 1451 Fragments arranged by size: 1766 1451 1272 936 635 623 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2922 3825 4509 5820 6218 6492 6639 7050 2922 Size: 903 684 1311 398 274 147 411 3259 Fragments arranged by size: 3259 1311 903 684 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2063 2093 1178 Size: 30 165 30 660 30 6472 Fragments arranged by size: 6472 660 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2087 2090 3176 3517 3665 3899 3992 4562 Size: 901 3 1086 341 148 234 93 570 Cuts at: 4562 1186 Size: 4011 Fragments arranged by size: 4011 1086 901 570 341 234 148 93 3 BseYI C'CCAG_C Cuts at: 2104 6590 7086 2104 Size: 4486 496 2405 Fragments arranged by size: 4486 2405 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2048 3029 1163 Size: 573 312 981 5521 Fragments arranged by size: 5521 981 573 312 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4837 5598 6626 1540 Size: 275 186 2836 761 1028 2301 Fragments arranged by size: 2836 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3408 3825 3995 4053 4161 4639 5448 5533 6604 Size: 417 170 58 108 478 809 85 1071 Cuts at: 6604 3408 Size: 4191 Fragments arranged by size: 4191 1071 809 478 417 170 108 85 58 BsiWI C'GTAC_G Cuts at: 2745 2745 Size: 7387 BsmI GAATG_Cn' Cuts at: 4898 4991 4898 Size: 93 7294 BsmBI CGTCTCn'nnnn_ Cuts at: 2755 4460 2755 Size: 1705 5682 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2022 2569 3322 3408 3825 3861 3957 Size: 399 232 547 753 86 417 36 96 Cuts at: 3957 3995 4015 4053 4161 4619 4639 4706 5448 Size: 38 20 38 108 458 20 67 742 Cuts at: 5448 5533 6604 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 753 742 547 458 417 399 232 108 96 86 85 67 38 38 36 20 20 BspEI T'CCGG_A Cuts at: 3825 4509 7050 3825 Size: 684 2541 4162 Fragments arranged by size: 4162 2541 684 BspHI T'CATG_A Cuts at: 2739 4327 4455 5276 7006 2739 Size: 1588 128 821 1730 3120 Fragments arranged by size: 3120 1730 1588 821 128 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2100 4483 4742 844 Size: 1256 2383 259 3489 Fragments arranged by size: 3489 2383 1256 259 BsrBI CCG'CTC Cuts at: 1394 1823 3718 5274 1394 Size: 429 1895 1556 3507 Fragments arranged by size: 3507 1895 1556 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2386 5882 6056 65 Size: 1183 1138 3496 174 1396 Fragments arranged by size: 3496 1396 1183 1138 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2168 2583 6031 6218 1700 Size: 110 358 415 3448 187 2869 Fragments arranged by size: 3448 2869 415 358 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7387 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7387 BssSI C'ACGA_G Cuts at: 3242 3335 3854 5447 6459 3242 Size: 93 519 1593 1012 4170 Fragments arranged by size: 4170 1593 1012 519 93 BstAPI GCAn_nnn'nTGC Cuts at: 794 2950 3274 3856 794 Size: 2156 324 582 4325 Fragments arranged by size: 4325 2156 582 324 BstBI TT'CG_AA Cuts at: 6191 6191 Size: 7387 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7387 BstYI r'GATC_y Cuts at: 1972 3270 3616 3654 3960 4227 4408 4512 4717 Size: 1298 346 38 306 267 181 104 205 Cuts at: 4717 5084 5469 5486 6938 7024 7036 7382 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1298 385 367 346 346 306 267 205 181 104 86 38 17 12 Bsu36I CC'TnA_GG Cuts at: 2159 2159 Size: 7387 BtgI C'CryG_G Cuts at: 513 1065 2649 3813 4356 513 Size: 552 1584 1164 543 3544 Fragments arranged by size: 3544 1584 1164 552 543 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3379 4180 4483 487 Size: 683 56 2153 801 303 3391 Fragments arranged by size: 3391 2153 801 683 303 56 BtsI GCAGTG_nn' Cuts at: 2136 3669 4482 4630 4903 5697 5717 2136 Size: 1533 813 148 273 794 20 3806 Fragments arranged by size: 3806 1533 813 794 273 148 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7387 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7352 DraI TTT'AAA Cuts at: 4691 4691 Size: 7387 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6394 809 Size: 507 5078 1802 Fragments arranged by size: 5078 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2646 3732 4834 Size: 54 1425 50 275 18 816 1086 1102 Cuts at: 4834 5723 7352 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1102 1086 889 816 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4834 1537 Size: 275 3022 4090 Fragments arranged by size: 4090 3022 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3494 4663 5317 1353 Size: 2141 1169 654 3423 Fragments arranged by size: 3423 2141 1169 654 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3722 4559 5974 6348 6494 387 Size: 1064 138 2133 837 1415 374 146 1280 Fragments arranged by size: 2133 1415 1280 1064 837 374 146 138 EcoICRI GAG'CTC Cuts at: 3406 3823 3993 3406 Size: 417 170 6800 Fragments arranged by size: 6800 417 170 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2185 2382 2718 2936 2984 3047 3158 Size: 393 553 197 336 218 48 63 111 Cuts at: 3158 3212 3581 4058 4109 5450 6047 6834 7102 Size: 54 369 477 51 1341 597 787 268 Cuts at: 7102 1239 Size: 1524 Fragments arranged by size: 1524 1341 787 597 553 477 393 369 336 268 218 197 111 63 54 51 48 EcoNI CCTnn'n_nnAGG Cuts at: 3130 3881 4544 3130 Size: 751 663 5973 Fragments arranged by size: 5973 751 663 EcoO109I rG'GnC_Cy Cuts at: 3954 4117 3954 Size: 163 7224 EcoRI G'AATT_C Cuts at: 4696 5074 4696 Size: 378 7009 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2570 2570 Size: 7387 FspI TGC'GCA Cuts at: 3675 5893 3675 Size: 2218 5169 HaeII r_GCGC'y Cuts at: 1678 2361 3199 3609 3925 6534 1678 Size: 683 838 410 316 2609 2531 Fragments arranged by size: 2609 2531 838 683 410 316 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3278 3284 3310 3316 3646 Size: 32 135 32 1706 6 26 6 330 Cuts at: 3646 3678 3707 3739 6028 6060 6102 6134 1373 Size: 32 29 32 2289 32 42 32 2626 Fragments arranged by size: 2626 2289 1706 330 135 42 32 32 32 32 32 32 29 26 6 6 HincII GTy'rAC Cuts at: 669 1657 1938 2305 4731 4977 669 Size: 988 281 367 2426 246 3079 Fragments arranged by size: 3079 2426 988 367 281 246 HindIII A'AGCT_T Cuts at: 748 2310 2609 748 Size: 1562 299 5526 Fragments arranged by size: 5526 1562 299 HpaI GTT'AAC Cuts at: 4977 4977 Size: 7387 KasI G'GCGC_C Cuts at: 3921 3921 Size: 7387 KpnI G_GTAC'C Cuts at: 4712 4712 Size: 7387 MfeI C'AATT_G Cuts at: 3592 4986 3592 Size: 1394 5993 MluI A'CGCG_T Cuts at: 7263 7263 Size: 7387 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2670 3090 4150 6501 6685 1305 Size: 1365 420 1060 2351 184 2007 Fragments arranged by size: 2351 2007 1365 1060 420 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2648 3734 10 Size: 54 1425 343 816 1086 3663 Fragments arranged by size: 3663 1425 1086 816 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4164 5345 5704 5863 518 Size: 648 2998 1181 359 159 2042 Fragments arranged by size: 2998 2042 1181 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2798 3025 4036 4803 5480 6628 6873 1606 Size: 1192 227 1011 767 677 1148 245 2120 Fragments arranged by size: 2120 1192 1148 1011 767 677 245 227 NaeI GCC'GGC Cuts at: 2170 2170 Size: 7387 NarI GG'CG_CC Cuts at: 3922 3922 Size: 7387 NcoI C'CATG_G Cuts at: 513 1065 2649 3813 4356 513 Size: 552 1584 1164 543 3544 Fragments arranged by size: 3544 1584 1164 552 543 NdeI CA'TA_TG Cuts at: 387 387 Size: 7387 NgoMIV G'CCGG_C Cuts at: 2168 2168 Size: 7387 NheI G'CTAG_C Cuts at: 1052 2869 1052 Size: 1817 5570 NotI GC'GGCC_GC Cuts at: 4834 4834 Size: 7387 NspI r_CATG'y Cuts at: 1132 1229 3905 4745 6290 1132 Size: 97 2676 840 1545 2229 Fragments arranged by size: 2676 2229 1545 840 97 PasI CC'CwG_GG Cuts at: 1348 2395 1348 Size: 1047 6340 PciI A'CATG_T Cuts at: 6286 6286 Size: 7387 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7387 PfoI T'CCnGG_A Cuts at: 2067 2097 2973 4110 2067 Size: 30 876 1137 5344 Fragments arranged by size: 5344 1137 876 30 PmeI GTTT'AAAC Cuts at: 4691 4691 Size: 7387 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3478 3510 5456 5488 6969 7001 3478 Size: 32 1946 32 1481 32 3864 Fragments arranged by size: 3864 1946 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2747 1613 Size: 1134 6253 PsiI TTA'TAA Cuts at: 4957 4957 Size: 7387 PspOMI G'GGCC_C Cuts at: 3953 4615 3953 Size: 662 6725 PstI C_TGCA'G Cuts at: 830 3051 3282 3882 4739 830 Size: 2221 231 600 857 3478 Fragments arranged by size: 3478 2221 857 600 231 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7387 PvuII CAG'CTG Cuts at: 3025 4036 3025 Size: 1011 6376 SacI G_AGCT'C Cuts at: 3408 3825 3995 3408 Size: 417 170 6800 Fragments arranged by size: 6800 417 170 SalI G'TCGA_C Cuts at: 1936 4729 1936 Size: 2793 4594 SbfI CC_TGCA'GG Cuts at: 3882 4739 3882 Size: 857 6530 ScaI AGT'ACT Cuts at: 4586 5635 7347 4586 Size: 1049 1712 4626 Fragments arranged by size: 4626 1712 1049 SfcI C'TryA_G Cuts at: 826 1045 3047 3278 3878 4735 5870 6551 6742 Size: 219 2002 231 600 857 1135 681 191 Cuts at: 6742 826 Size: 1471 Fragments arranged by size: 2002 1471 1135 857 681 600 231 219 191 SfoI GGC'GCC Cuts at: 3923 3923 Size: 7387 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7387 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7387 SmaI CCC'GGG Cuts at: 1872 4714 1872 Size: 2842 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2213 3787 5491 6392 6654 820 Size: 197 926 270 1574 1704 901 262 1553 Fragments arranged by size: 1704 1574 1553 926 901 270 262 197 SnaBI TAC'GTA Cuts at: 493 5178 493 Size: 4685 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7387 SphI G_CATG'C Cuts at: 1132 3905 4745 1132 Size: 2773 840 3774 Fragments arranged by size: 3774 2773 840 SspI AAT'ATT Cuts at: 5 52 2638 5311 5 Size: 47 2586 2673 2081 Fragments arranged by size: 2673 2586 2081 47 StuI AGG'CCT Cuts at: 1517 3233 1517 Size: 1716 5671 StyI C'CwwG_G Cuts at: 513 1065 2554 2649 3003 3813 4356 4826 513 Size: 552 1489 95 354 810 543 470 3074 Fragments arranged by size: 3074 1489 810 552 543 470 354 95 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2189 3016 3492 4271 4361 5414 5431 Size: 1255 609 827 476 779 90 1053 17 Cuts at: 5431 5584 5769 6214 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 1053 827 779 609 476 445 185 153 90 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2031 2058 4584 Size: 274 80 33 51 1443 54 27 2526 Cuts at: 4584 5633 7345 96 Size: 1049 1712 138 Fragments arranged by size: 2526 1712 1443 1049 274 138 80 54 51 33 27 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2063 2095 7347 7379 2063 Size: 32 5252 32 2071 Fragments arranged by size: 5252 2071 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7387 XbaI T'CTAG_A Cuts at: 4723 4723 Size: 7387 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4213 4213 Size: 7387 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7387 XmaI C'CCGG_G Cuts at: 1870 4712 1870 Size: 2842 4545 XmnI GAAnn'nnTTC Cuts at: 2132 2892 4274 5516 2132 Size: 760 1382 1242 4003 Fragments arranged by size: 4003 1382 1242 760 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5195 276 Size: 53 83 186 936 3661 2468 Fragments arranged by size: 3661 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BstXI BstZ17I DraIII EcoRV FseI FspAI NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI