(Linear) (Six-base) MAPSORT of: KIEE2106.seq Check: 9969 from: 1 to: 2682 KIEE2106.orf With 182 enzymes: SgfI * July 15, 2010 18:54 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 180 377 713 2053 2682 Size: 180 197 336 1340 629 Fragments arranged by size: 1340 629 336 197 180 AfeI AGC'GCT Cuts at: 0 1192 2682 Size: 1192 1490 AflII C'TTAA_G Cuts at: 0 208 2682 Size: 208 2474 AflIII A'CryG_T Cuts at: 0 35 2682 Size: 35 2647 AleI CACnn'nnGTG Cuts at: 0 2159 2682 Size: 2159 523 AlwNI CAG_nnn'CTG Cuts at: 0 1131 1659 2226 2339 2682 Size: 1131 528 567 113 343 Fragments arranged by size: 1131 567 528 343 113 ApaI G_GGCC'C Cuts at: 0 1952 2614 2682 Size: 1952 662 68 Fragments arranged by size: 1952 662 68 ApaLI G'TGCA_C Cuts at: 0 2630 2682 Size: 2630 52 ApoI r'AATT_y Cuts at: 0 1454 2377 2682 Size: 1454 923 305 Fragments arranged by size: 1454 923 305 AvaI C'yCGr_G Cuts at: 0 925 1696 2607 2682 Size: 925 771 911 75 Fragments arranged by size: 925 911 771 75 BamHI G'GATC_C Cuts at: 0 1955 2507 2682 Size: 1955 552 175 Fragments arranged by size: 1955 552 175 BanI G'GyrC_C Cuts at: 0 166 1117 1445 1916 2419 2682 Size: 166 951 328 471 503 263 Fragments arranged by size: 951 503 471 328 263 166 BanII G_rGCy'C Cuts at: 0 17 1317 1403 1820 1952 1990 2010 2614 Size: 17 1300 86 417 132 38 20 604 Cuts at: 2614 2682 Size: 68 Fragments arranged by size: 1300 604 417 132 86 68 38 20 17 BbeI G_GCGC'C Cuts at: 0 1920 2682 Size: 1920 762 BbsI GAAGACnn'nnnn_ Cuts at: 0 130 197 701 937 2021 2682 Size: 130 67 504 236 1084 661 Fragments arranged by size: 1084 661 504 236 130 67 BclI T'GATC_A Cuts at: 0 1542 1661 2682 Size: 1542 119 1021 Fragments arranged by size: 1542 1021 119 BglI GCCn_nnn'nGGC Cuts at: 0 1570 2682 Size: 1570 1112 BglII A'GATC_T Cuts at: 0 1265 2682 Size: 1265 1417 Bme1580I G_kGCm'C Cuts at: 0 564 1856 1952 2614 2634 2682 Size: 564 1292 96 662 20 48 Fragments arranged by size: 1292 662 564 96 48 20 BmrI ACTGGGnnnn_n' Cuts at: 0 1486 2571 2682 Size: 1486 1085 111 Fragments arranged by size: 1486 1085 111 BmtI G_CTAG'C Cuts at: 0 868 2682 Size: 868 1814 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1592 2682 Size: 1592 1090 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 931 979 1042 1153 1207 1576 2104 2682 Size: 931 48 63 111 54 369 528 578 Fragments arranged by size: 931 578 528 369 111 63 54 48 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1767 2682 Size: 1767 915 BsaI GGTCTCn'nnnn_ Cuts at: 0 2084 2682 Size: 2084 598 BsaAI yAC'GTr Cuts at: 0 36 2682 Size: 36 2646 BsaHI Gr'CG_yC Cuts at: 0 1294 1917 2682 Size: 1294 623 765 Fragments arranged by size: 1294 765 623 BsaWI w'CCGG_w Cuts at: 0 917 1820 2504 2682 Size: 917 903 684 178 Fragments arranged by size: 917 903 684 178 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 58 88 2682 Size: 58 30 2594 Fragments arranged by size: 2594 58 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 82 85 1171 1512 1660 1894 1987 2557 Size: 82 3 1086 341 148 234 93 570 Cuts at: 2557 2682 Size: 125 Fragments arranged by size: 1086 570 341 234 148 125 93 82 3 BseYI C'CCAG_C Cuts at: 0 99 2682 Size: 99 2583 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 43 1024 2682 Size: 43 981 1658 Fragments arranged by size: 1658 981 43 BsiHKAI G_wGCw'C Cuts at: 0 1403 1820 1990 2048 2156 2634 2682 Size: 1403 417 170 58 108 478 48 Fragments arranged by size: 1403 478 417 170 108 58 48 BsiWI C'GTAC_G Cuts at: 0 740 2682 Size: 740 1942 BsmBI CGTCTCn'nnnn_ Cuts at: 0 750 2455 2682 Size: 750 1705 227 Fragments arranged by size: 1705 750 227 Bsp1286I G_dGCh'C Cuts at: 0 17 564 1317 1403 1820 1856 1952 1990 Size: 17 547 753 86 417 36 96 38 Cuts at: 1990 2010 2048 2156 2614 2634 2682 Size: 20 38 108 458 20 48 Fragments arranged by size: 753 547 458 417 108 96 86 48 38 38 36 20 20 17 BspEI T'CCGG_A Cuts at: 0 1820 2504 2682 Size: 1820 684 178 Fragments arranged by size: 1820 684 178 BspHI T'CATG_A Cuts at: 0 734 2322 2450 2682 Size: 734 1588 128 232 Fragments arranged by size: 1588 734 232 128 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 95 2478 2682 Size: 95 2383 204 Fragments arranged by size: 2383 204 95 BsrBI CCG'CTC Cuts at: 0 1713 2682 Size: 1713 969 BsrDI GCAATG_nn' Cuts at: 0 381 2682 Size: 381 2301 BsrFI r'CCGG_y Cuts at: 0 163 578 2682 Size: 163 415 2104 Fragments arranged by size: 2104 415 163 BssSI C'ACGA_G Cuts at: 0 1237 1330 1849 2682 Size: 1237 93 519 833 Fragments arranged by size: 1237 833 519 93 BstAPI GCAn_nnn'nTGC Cuts at: 0 945 1269 1851 2682 Size: 945 324 582 831 Fragments arranged by size: 945 831 582 324 BstYI r'GATC_y Cuts at: 0 1265 1611 1649 1955 2222 2403 2507 2682 Size: 1265 346 38 306 267 181 104 175 Fragments arranged by size: 1265 346 306 267 181 175 104 38 Bsu36I CC'TnA_GG Cuts at: 0 154 2682 Size: 154 2528 BtgI C'CryG_G Cuts at: 0 644 1808 2351 2682 Size: 644 1164 543 331 Fragments arranged by size: 1164 644 543 331 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1374 2175 2478 2682 Size: 1374 801 303 204 Fragments arranged by size: 1374 801 303 204 BtsI GCAGTG_nn' Cuts at: 0 131 1664 2477 2625 2682 Size: 131 1533 813 148 57 Fragments arranged by size: 1533 813 148 131 57 EaeI y'GGCC_r Cuts at: 0 641 1727 2682 Size: 641 1086 955 Fragments arranged by size: 1086 955 641 EarI CTCTTCn'nnn_ Cuts at: 0 1489 2658 2682 Size: 1489 1169 24 Fragments arranged by size: 1489 1169 24 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1717 2554 2682 Size: 1717 837 128 Fragments arranged by size: 1717 837 128 EcoICRI GAG'CTC Cuts at: 0 1401 1818 1988 2682 Size: 1401 417 170 694 Fragments arranged by size: 1401 694 417 170 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 180 377 713 931 979 1042 1153 1207 Size: 180 197 336 218 48 63 111 54 Cuts at: 1207 1576 2053 2104 2682 Size: 369 477 51 578 Fragments arranged by size: 578 477 369 336 218 197 180 111 63 54 51 48 EcoNI CCTnn'n_nnAGG Cuts at: 0 1125 1876 2539 2682 Size: 1125 751 663 143 Fragments arranged by size: 1125 751 663 143 EcoO109I rG'GnC_Cy Cuts at: 0 1949 2112 2682 Size: 1949 163 570 Fragments arranged by size: 1949 570 163 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 565 2682 Size: 565 2117 FspI TGC'GCA Cuts at: 0 1670 2682 Size: 1670 1012 HaeII r_GCGC'y Cuts at: 0 356 1194 1604 1920 2682 Size: 356 838 410 316 762 Fragments arranged by size: 838 762 410 356 316 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1273 1279 1305 1311 1641 1673 1702 1734 Size: 1273 6 26 6 330 32 29 32 Cuts at: 1734 2682 Size: 948 Fragments arranged by size: 1273 948 330 32 32 29 26 6 6 HincII GTy'rAC Cuts at: 0 300 2682 Size: 300 2382 HindIII A'AGCT_T Cuts at: 0 305 604 2682 Size: 305 299 2078 Fragments arranged by size: 2078 305 299 KasI G'GCGC_C Cuts at: 0 1916 2682 Size: 1916 766 MfeI C'AATT_G Cuts at: 0 1587 2682 Size: 1587 1095 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 665 1085 2145 2682 Size: 665 420 1060 537 Fragments arranged by size: 1060 665 537 420 MscI TGG'CCA Cuts at: 0 643 1729 2682 Size: 643 1086 953 Fragments arranged by size: 1086 953 643 MslI CAynn'nnrTG Cuts at: 0 2159 2682 Size: 2159 523 MspA1I CmG'CkG Cuts at: 0 793 1020 2031 2682 Size: 793 227 1011 651 Fragments arranged by size: 1011 793 651 227 NaeI GCC'GGC Cuts at: 0 165 2682 Size: 165 2517 NarI GG'CG_CC Cuts at: 0 1917 2682 Size: 1917 765 NcoI C'CATG_G Cuts at: 0 644 1808 2351 2682 Size: 644 1164 543 331 Fragments arranged by size: 1164 644 543 331 NgoMIV G'CCGG_C Cuts at: 0 163 2682 Size: 163 2519 NheI G'CTAG_C Cuts at: 0 864 2682 Size: 864 1818 NspI r_CATG'y Cuts at: 0 1900 2682 Size: 1900 782 PasI CC'CwG_GG Cuts at: 0 390 2682 Size: 390 2292 PfoI T'CCnGG_A Cuts at: 0 62 92 968 2105 2682 Size: 62 30 876 1137 577 Fragments arranged by size: 1137 876 577 62 30 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1473 1505 2682 Size: 1473 32 1177 Fragments arranged by size: 1473 1177 32 PshAI GACnn'nnGTC Cuts at: 0 742 2682 Size: 742 1940 PspOMI G'GGCC_C Cuts at: 0 1948 2610 2682 Size: 1948 662 72 Fragments arranged by size: 1948 662 72 PstI C_TGCA'G Cuts at: 0 1046 1277 1877 2682 Size: 1046 231 600 805 Fragments arranged by size: 1046 805 600 231 PvuII CAG'CTG Cuts at: 0 1020 2031 2682 Size: 1020 1011 651 Fragments arranged by size: 1020 1011 651 SacI G_AGCT'C Cuts at: 0 1403 1820 1990 2682 Size: 1403 417 170 692 Fragments arranged by size: 1403 692 417 170 SbfI CC_TGCA'GG Cuts at: 0 1877 2682 Size: 1877 805 ScaI AGT'ACT Cuts at: 0 2581 2682 Size: 2581 101 SfcI C'TryA_G Cuts at: 0 1042 1273 1873 2682 Size: 1042 231 600 809 Fragments arranged by size: 1042 809 600 231 SfoI GGC'GCC Cuts at: 0 1918 2682 Size: 1918 764 SmlI C'TyrA_G Cuts at: 0 208 1782 2682 Size: 208 1574 900 Fragments arranged by size: 1574 900 208 SphI G_CATG'C Cuts at: 0 1900 2682 Size: 1900 782 SspI AAT'ATT Cuts at: 0 633 2682 Size: 633 2049 StuI AGG'CCT Cuts at: 0 1228 2682 Size: 1228 1454 StyI C'CwwG_G Cuts at: 0 549 644 998 1808 2351 2682 Size: 549 95 354 810 543 331 Fragments arranged by size: 810 549 543 354 331 95 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 184 1011 1487 2266 2356 2682 Size: 184 827 476 779 90 326 Fragments arranged by size: 827 779 476 326 184 90 TatI w'GTAC_w Cuts at: 0 26 53 2579 2682 Size: 26 27 2526 103 Fragments arranged by size: 2526 103 27 26 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 58 90 2682 Size: 58 32 2592 Fragments arranged by size: 2592 58 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 2208 2682 Size: 2208 474 XmnI GAAnn'nnTTC Cuts at: 0 127 887 2269 2682 Size: 127 760 1382 413 Fragments arranged by size: 1382 760 413 127 Enzymes that do cut: AcuI AfeI AflII AflIII AleI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII BbeI BbsI BclI BglI BglII Bme1580I BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstYI Bsu36I BtgI BtgZI BtsI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I HincII HindIII KasI MfeI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI PasI PfoI PpiI PshAI PspOMI PstI PvuII SacI SbfI ScaI SfcI SfoI SmlI SphI SspI StuI StyI TaqII TatI TstI XcmI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AgeI AhdI AlfI AloI AscI AseI AsiSI AvrII BaeI BbvCI BcgI BciVI BfrBI BlpI BmgBI Bpu10I BsaBI BsiEI BsmI BsrGI BssHII BstBI BstEII BstXI BstZ17I ClaI CspCI DraI DraIII DrdI EagI EcoRI EcoRV FseI FspAI HpaI KpnI MluI NdeI NotI NruI NsiI PacI PciI PflMI PmeI PmlI PpuMI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI SwaI Tth111I XbaI XhoI XmaI ZraI