Restriction map for
pFN21AE2101
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# NO CUT ENZYMES ( 128 enzymes) #
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AatII Acc65I AccII AclI AcyI
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AviII BamHI BanI BanII BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BglI BlpI Bpu1102I BsaBI
BsaHI BsaI BseRI BsgI BsiEI
BsiWI BsmBI Bsp1407I BspDI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HpaI KasI KpnI KspI
MamI MfeI MluI MspA1I MunI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PflMI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SpeI SphI
Sse8387I SspBI StuI SwaI Van91I
XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 671 GTAGAC GT^[AC][GT]AC
AccIII 1058 TCCGGA T^CCGGA
AflII 85 CTTAAG C^TTAAG
AflIII 463 ACATGT A^C[GA][CT]GT
AseI 789 ATTAAT AT^TAAT
AsnI 789 ATTAAT AT^TAAT
Asp700I 2164 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 525 GAGCAC G[AT]GC[AT]^C
AspI 113 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 2117 CTCGAG C^[CT]CG[GA]G
AvrII 826 CCTAGG C^CTAGG
BalI 1502 TGGCCA TGG^CCA
BfrI 85 CTTAAG C^TTAAG
BglII 1590 AGATCT A^GATCT
BlnI 826 CCTAGG C^CTAGG
BmyI 525 GAGCAC G[AGT]GC[ACT]^C
BseAI 1058 TCCGGA T^CCGGA
BsiHKAI 525 GAGCAC G[AT]GC[AT]^C
BsmI 2228 GAATGC GAATGC(1/-1)
BsoBI 2117 CTCGAG C^[CT]CG[GA]G
Bsp1286I 525 GAGCAC G[AGT]GC[ACT]^C
BspEI 1058 TCCGGA T^CCGGA
BspHI 2028 TCATGA T^CATGA
BspLU11I 463 ACATGT A^CATGT
BstAPI 2178 GCAATATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cac8I 1739 GCTTGC GC[ACGT]^[ACGT]GC
DraI 619 TTTAAA TTT^AAA
EaeI 1502 TGGCCA [CT]^GGCC[GA]
EcoRI 1352 GAATTC G^AATTC
HindIII 2141 AAGCTT A^AGCTT
MluNI 1502 TGGCCA TGG^CCA
MroI 1058 TCCGGA T^CCGGA
MscI 1502 TGGCCA TGG^CCA
PaeR7I 2117 CTCGAG C^TCGAG
PflFI 113 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 2213 AGGTCCT [GA]G^G[AT]CC[CT]
PshBI 789 ATTAAT AT^TAAT
PstI 2359 CTGCAG CTGCA^G
RcaI 2028 TCATGA T^CATGA
SexAI 864 ACCTGGT A^CC[AT]GGT
SnaBI 124 TACGTA TAC^GTA
TaqI 2118 TCGA T^CGA
Tth111I 113 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 2118 TCGA T^CGA
XbaI 628 TCTAGA T^CTAGA
XhoI 2117 CTCGAG C^TCGAG
XmnI 2164 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AfaI 728 GTAC GT^AC
1986 GTAC
BpmI 498 CTCCAG CTGGAG(16/14)
812 CTGGAG
BsaAI 124 TACGTA [CT]AC^GT[GA]
668 CACGTA
BsaWI 1058 TCCGGA [AT]^CCGG[AT]
1481 TCCGGT
BspMI 2377 ACCTGC ACCTGC(4/8)
2504 ACCTGC
BstXI 862 CCACCTGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1198 CCACCTGGATGG
DraII 822 AGGCCCT [GA]G^G[ACGT]CC[CT]
2213 AGGTCCT
DsaI 1814 CCATGG C^C[GA][CT]GG
1827 CCATGG
EarI 1212 GAAGAG CTCTTC(1/4)
2343 GAAGAG
EcoO109I 822 AGGCCCT [GA]G^G[ACGT]CC[CT]
2213 AGGTCCT
HapII 1059 CCGG C^CGG
1482 CCGG
HpaII 1059 CCGG C^CGG
1482 CCGG
Ksp632I 1212 GAAGAG CTCTTC(1/4)
2343 GAAGAG
MspI 1059 CCGG C^CGG
1482 CCGG
NcoI 1814 CCATGG C^CATGG
1827 CCATGG
NlaIV 496 GGCTCC GG[ACGT]^[ACGT]CC
2374 GGAACC
NspI 463 ACATGT [GA]CATG^[CT]
2262 ACATGC
RsaI 728 GTAC GT^AC
1986 GTAC
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AcsI AluI AlwI AlwNI
ApoI AvaII BbsI BbvI BfaI
BpuAI BsaJI BsiYI BslI BsmAI
BsmFI BsrDI BsrI BssKI BstF5I
BstNI BstYI Cfr13I DdeI DpnII
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HincII HindII HinfI HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MvaI MwoI NdeII NlaIII PleI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII