(Circular) (Six-base) MAPSORT of: pFN21AE0396.seq Check: 5002 from: 1 to: 7825 pFN21AE0396 7825 bp With 182 enzymes: SgfI * April 15, 2010 09:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3465 3465 Size: 7825 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5635 278 Size: 53 83 186 936 4099 2468 Fragments arranged by size: 4099 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5168 1937 Size: 3231 4594 Acc65I G'GTAC_C Cuts at: 5146 5146 Size: 7825 AclI AA'CG_TT Cuts at: 1563 5952 6325 1563 Size: 4389 373 3063 Fragments arranged by size: 4389 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2833 3272 4001 4785 4961 4988 5888 7272 Size: 1201 439 729 784 176 27 900 1384 Cuts at: 7272 7540 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1201 900 784 729 439 268 176 27 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7628 AflIII A'CryG_T Cuts at: 1203 2608 3693 6724 7701 1203 Size: 1405 1085 3031 977 1327 Fragments arranged by size: 3031 1405 1327 1085 977 AgeI A'CCGG_T Cuts at: 6656 6656 Size: 7825 AhdI GACnn_n'nnGTC Cuts at: 2347 3279 6554 2347 Size: 932 3275 3618 Fragments arranged by size: 3618 3275 932 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7825 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2945 1184 Size: 1761 6064 AlwNI CAG_nnn'CTG Cuts at: 2938 3220 3499 4435 4714 4759 5568 7140 2938 Size: 282 279 936 279 45 809 1572 3623 Fragments arranged by size: 3623 1572 936 809 282 279 279 45 ApaI G_GGCC'C Cuts at: 4331 4753 4331 Size: 422 7403 ApaLI G'TGCA_C Cuts at: 4581 5882 7038 4581 Size: 1301 1156 5368 Fragments arranged by size: 5368 1301 1156 ApoI r'AATT_y Cuts at: 1493 2975 3803 4948 5134 5360 5512 7621 1493 Size: 1482 828 1145 186 226 152 2109 1697 Fragments arranged by size: 2109 1697 1482 1145 828 226 186 152 AseI AT'TA_AT Cuts at: 160 1032 6379 160 Size: 872 5347 1606 Fragments arranged by size: 5347 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7825 AvaI C'yCGr_G Cuts at: 1870 1943 2397 3392 4053 4214 5150 1870 Size: 73 454 995 661 161 936 4545 Fragments arranged by size: 4545 995 936 661 454 161 73 AvrII C'CTAG_G Cuts at: 4996 4996 Size: 7825 BamHI G'GATC_C Cuts at: 5155 5522 5155 Size: 367 7458 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2262 3793 4110 4299 4832 Size: 325 217 627 475 1531 317 189 533 Cuts at: 4832 5146 6601 618 Size: 314 1455 1842 Fragments arranged by size: 1842 1531 1455 627 533 475 325 317 314 217 189 BanII G_rGCy'C Cuts at: 1391 2822 2847 3393 4331 4669 4689 4753 5144 Size: 1431 25 546 938 338 20 64 391 Cuts at: 5144 1391 Size: 4072 Fragments arranged by size: 4072 1431 938 546 391 338 64 25 20 BbeI G_GCGC'C Cuts at: 2266 3797 4114 2266 Size: 1531 317 5977 Fragments arranged by size: 5977 1531 317 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2980 3093 3457 3611 928 Size: 971 1081 113 364 154 5142 Fragments arranged by size: 5142 1081 971 364 154 113 BbvCI CC'TCA_GC Cuts at: 2681 3433 2681 Size: 752 7073 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4482 4516 6016 6050 1903 Size: 34 2545 34 1500 34 3678 Fragments arranged by size: 3678 2545 1500 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5717 6927 5717 Size: 1210 6615 BclI T'GATC_A Cuts at: 1548 4777 1548 Size: 3229 4596 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4037 6436 136 Size: 107 122 71 631 605 2365 2399 1525 Fragments arranged by size: 2399 2365 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7820 7820 Size: 7825 BlpI GC'TnA_GC Cuts at: 4596 4799 5242 4596 Size: 203 443 7179 Fragments arranged by size: 7179 443 203 Bme1580I G_kGCm'C Cuts at: 1790 4126 4302 4331 4585 4753 4837 5886 7042 Size: 2336 176 29 254 168 84 1049 1156 Cuts at: 7042 1790 Size: 2573 Fragments arranged by size: 2573 2336 1156 1049 254 176 168 84 29 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7825 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3609 4072 5556 Size: 416 53 83 399 24 2192 463 1484 Cuts at: 5556 6514 442 Size: 958 1753 Fragments arranged by size: 2192 1753 1484 958 463 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7825 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2218 2218 Size: 7825 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2768 2795 2858 3698 4889 4910 6485 1239 Size: 1529 27 63 840 1191 21 1575 2579 Fragments arranged by size: 2579 1575 1529 1191 840 63 27 21 Bpu10I CC'TnA_GC Cuts at: 2681 3433 2681 Size: 752 7073 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3320 4289 4810 5950 6815 7113 3320 Size: 969 521 1140 865 298 4032 Fragments arranged by size: 4032 1140 969 865 521 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2146 2394 3422 6488 882 Size: 617 647 248 1028 3066 2219 Fragments arranged by size: 3066 2219 1028 647 617 248 BsaAI yAC'GTr Cuts at: 493 1204 3694 4283 5616 493 Size: 711 2490 589 1333 2702 Fragments arranged by size: 2702 2490 1333 711 589 BsaBI GATnn'nnATC Cuts at: 3947 7819 3947 Size: 3872 3953 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2263 3794 4111 5632 Size: 53 83 186 936 730 1531 317 1521 Cuts at: 5632 6014 6649 275 Size: 382 635 1451 Fragments arranged by size: 1531 1521 1451 936 730 635 382 317 186 83 53 BsaWI w'CCGG_w Cuts at: 4233 6258 6656 6930 7077 7488 4233 Size: 2025 398 274 147 411 4570 Fragments arranged by size: 4570 2025 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3503 3533 4148 4178 4656 Size: 30 165 30 2100 30 615 30 478 Cuts at: 4656 4686 1178 Size: 30 4317 Fragments arranged by size: 4317 2100 615 478 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2051 2054 2131 2855 4271 4907 5096 1186 Size: 865 3 77 724 1416 636 189 3915 Fragments arranged by size: 3915 1416 865 724 636 189 77 3 BseYI C'CCAG_C Cuts at: 2370 2392 2821 3705 4636 7028 7524 2370 Size: 22 429 884 931 2392 496 2671 Fragments arranged by size: 2671 2392 931 884 496 429 22 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2924 3425 3632 4164 4263 1163 Size: 573 1188 501 207 532 99 4725 Fragments arranged by size: 4725 1188 573 532 501 207 99 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3198 3288 5275 6036 7064 1540 Size: 275 186 1197 90 1987 761 1028 2301 Fragments arranged by size: 2301 1987 1197 1028 761 275 186 90 BsiHKAI G_wGCw'C Cuts at: 2093 2847 2924 3060 4062 4522 4585 5886 5971 Size: 754 77 136 1002 460 63 1301 85 Cuts at: 5971 7042 2093 Size: 1071 2876 Fragments arranged by size: 2876 1301 1071 1002 754 460 136 85 77 63 BsmI GAATG_Cn' Cuts at: 3262 5336 5429 3262 Size: 2074 93 5658 Fragments arranged by size: 5658 2074 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2729 2729 Size: 7825 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2093 2822 2847 2924 3060 3393 4062 Size: 399 303 729 25 77 136 333 669 Cuts at: 4062 4126 4302 4331 4522 4585 4669 4689 4753 Size: 64 176 29 191 63 84 20 64 Cuts at: 4753 4837 5144 5886 5971 7042 1391 Size: 84 307 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 729 669 399 333 307 303 191 176 136 85 84 84 77 64 64 63 29 25 20 BspEI T'CCGG_A Cuts at: 4233 7488 4233 Size: 3255 4570 BspHI T'CATG_A Cuts at: 3385 5714 7444 3385 Size: 2329 1730 3766 Fragments arranged by size: 3766 2329 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2412 3255 3465 3555 5180 844 Size: 1568 843 210 90 1625 3489 Fragments arranged by size: 3489 1625 1568 843 210 90 BsrBI CCG'CTC Cuts at: 1394 1823 2479 4261 5712 1394 Size: 429 656 1782 1451 3507 Fragments arranged by size: 3507 1782 1451 656 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6320 6494 65 Size: 1183 5072 174 1396 Fragments arranged by size: 5072 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2415 3003 6469 6656 1700 Size: 110 605 588 3466 187 2869 Fragments arranged by size: 3466 2869 605 588 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7825 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7825 BssSI C'ACGA_G Cuts at: 5885 6897 5885 Size: 1012 6813 BstAPI GCAn_nnn'nTGC Cuts at: 794 2938 3568 3687 3857 4210 4435 794 Size: 2144 630 119 170 353 225 4184 Fragments arranged by size: 4184 2144 630 353 225 170 119 BstBI TT'CG_AA Cuts at: 6629 6629 Size: 7825 BstEII G'GTnAC_C Cuts at: 1184 3271 1184 Size: 2087 5738 BstYI r'GATC_y Cuts at: 1972 5155 5522 5907 5924 7376 7462 7474 7820 Size: 3183 367 385 17 1452 86 12 346 Cuts at: 7820 1972 Size: 1977 Fragments arranged by size: 3183 1977 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3305 3371 3305 Size: 66 7759 BtgI C'CryG_G Cuts at: 513 1065 2004 2568 2688 4380 4590 4659 513 Size: 552 939 564 120 1692 210 69 3679 Fragments arranged by size: 3679 1692 939 564 552 210 120 69 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2299 487 Size: 683 56 1073 6013 Fragments arranged by size: 6013 1073 683 56 BtsI GCAGTG_nn' Cuts at: 2917 3020 3058 3184 4327 4525 4653 4831 5341 Size: 103 38 126 1143 198 128 178 510 Cuts at: 5341 6135 6155 2917 Size: 794 20 4587 Fragments arranged by size: 4587 1143 794 510 198 178 128 126 103 38 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7825 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3858 3893 529 Size: 35 3294 35 4461 Fragments arranged by size: 4461 3294 35 35 DraI TTT'AAA Cuts at: 5129 5129 Size: 7825 DraIII CAC_nnn'GTG Cuts at: 3857 3945 4187 4338 3857 Size: 88 242 151 7344 Fragments arranged by size: 7344 242 151 88 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2692 6832 809 Size: 507 1376 4140 1802 Fragments arranged by size: 4140 1802 1376 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2256 2417 2673 Size: 54 1425 50 275 18 426 161 256 Cuts at: 2673 2860 3021 3285 3924 4677 5272 6161 7790 Size: 187 161 264 639 753 595 889 1629 Cuts at: 7790 8 Size: 43 Fragments arranged by size: 1629 1425 889 753 639 595 426 275 264 256 187 161 161 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3285 5272 1537 Size: 275 1473 1987 4090 Fragments arranged by size: 4090 1987 1473 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2007 2810 2852 3230 3896 4527 5755 1353 Size: 654 803 42 378 666 631 1228 3423 Fragments arranged by size: 3423 1228 803 666 654 631 378 42 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3404 3416 6412 6786 6932 387 Size: 1064 138 1815 12 2996 374 146 1280 Fragments arranged by size: 2996 1815 1280 1064 374 146 138 12 EcoICRI GAG'CTC Cuts at: 2845 2845 Size: 7825 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2768 2795 2833 2858 3272 3698 4001 Size: 393 1136 27 38 25 414 426 303 Cuts at: 4001 4785 4889 4910 4961 4988 5888 6485 7272 Size: 784 104 21 51 27 900 597 787 Cuts at: 7272 7540 1239 Size: 268 1524 Fragments arranged by size: 1524 1136 900 787 784 597 426 414 393 303 268 104 51 38 27 27 25 21 EcoNI CCTnn'n_nnAGG Cuts at: 3236 5015 3236 Size: 1779 6046 EcoO109I rG'GnC_Cy Cuts at: 2148 2197 2374 3519 3611 3673 4066 4099 4328 Size: 49 177 1145 92 62 393 33 229 Cuts at: 4328 4749 4756 5020 2148 Size: 421 7 264 4953 Fragments arranged by size: 4953 1145 421 393 264 229 177 92 62 49 33 7 EcoRI G'AATT_C Cuts at: 3803 5134 5512 3803 Size: 1331 378 6116 Fragments arranged by size: 6116 1331 378 FspI TGC'GCA Cuts at: 6331 6331 Size: 7825 HaeII r_GCGC'y Cuts at: 1678 2266 3382 3797 4114 6972 1678 Size: 588 1116 415 317 2858 2531 Fragments arranged by size: 2858 2531 1116 588 415 317 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2040 2072 2333 2365 2366 Size: 32 135 32 468 32 261 32 1 Cuts at: 2366 2398 3191 3223 3265 3297 3503 3535 4388 Size: 32 793 32 42 32 206 32 853 Cuts at: 4388 4420 6466 6498 6540 6572 1373 Size: 32 2046 32 42 32 2626 Fragments arranged by size: 2626 2046 853 793 468 261 206 135 42 42 32 32 32 32 32 32 32 32 32 32 32 1 HincII GTy'rAC Cuts at: 669 1657 1938 3132 4311 5089 5169 5415 669 Size: 988 281 1194 1179 778 80 246 3079 Fragments arranged by size: 3079 1194 1179 988 778 281 246 80 HindIII A'AGCT_T Cuts at: 748 748 Size: 7825 HpaI GTT'AAC Cuts at: 3132 5415 3132 Size: 2283 5542 KasI G'GCGC_C Cuts at: 2262 3793 4110 2262 Size: 1531 317 5977 Fragments arranged by size: 5977 1531 317 KpnI G_GTAC'C Cuts at: 5150 5150 Size: 7825 MfeI C'AATT_G Cuts at: 3895 4535 5424 3895 Size: 640 889 6296 Fragments arranged by size: 6296 889 640 MluI A'CGCG_T Cuts at: 7701 7701 Size: 7825 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6939 7123 1305 Size: 5634 184 2007 Fragments arranged by size: 5634 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2258 2675 2862 3023 3926 Size: 54 1425 343 426 417 187 161 903 Cuts at: 3926 10 Size: 3909 Fragments arranged by size: 3909 1425 903 426 417 343 187 161 54 MslI CAynn'nnrTG Cuts at: 518 1166 2445 2868 4421 5783 6142 6301 518 Size: 648 1279 423 1553 1362 359 159 2042 Fragments arranged by size: 2042 1553 1362 1279 648 423 359 159 MspA1I CmG'CkG Cuts at: 1606 2125 2935 3000 3010 3290 4128 4178 4393 Size: 519 810 65 10 280 838 50 215 Cuts at: 4393 4432 4661 5241 5918 7066 7311 1606 Size: 39 229 580 677 1148 245 2120 Fragments arranged by size: 2120 1148 838 810 677 580 519 280 245 229 215 65 50 39 10 NaeI GCC'GGC Cuts at: 3005 3005 Size: 7825 NarI GG'CG_CC Cuts at: 2263 3794 4111 2263 Size: 1531 317 5977 Fragments arranged by size: 5977 1531 317 NcoI C'CATG_G Cuts at: 513 1065 2004 4590 513 Size: 552 939 2586 3748 Fragments arranged by size: 3748 2586 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7825 NgoMIV G'CCGG_C Cuts at: 3003 3003 Size: 7825 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7825 NotI GC'GGCC_GC Cuts at: 5272 5272 Size: 7825 NspI r_CATG'y Cuts at: 1132 1229 2461 2612 2661 3578 3685 4208 4611 Size: 97 1232 151 49 917 107 523 403 Cuts at: 4611 5183 6728 1132 Size: 572 1545 2229 Fragments arranged by size: 2229 1545 1232 917 572 523 403 151 107 97 49 PasI CC'CwG_GG Cuts at: 1348 2830 1348 Size: 1482 6343 PciI A'CATG_T Cuts at: 2608 6724 2608 Size: 4116 3709 PflMI CCAn_nnn'nTGG Cuts at: 1108 2233 3607 4675 4759 4843 1108 Size: 1125 1374 1068 84 84 4090 Fragments arranged by size: 4090 1374 1125 1068 84 84 PfoI T'CCnGG_A Cuts at: 2172 2772 2970 4905 5043 2172 Size: 600 198 1935 138 4954 Fragments arranged by size: 4954 1935 600 198 138 PmeI GTTT'AAAC Cuts at: 5129 5129 Size: 7825 PmlI CAC'GTG Cuts at: 4283 4283 Size: 7825 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3143 3175 5057 5089 5894 5926 7407 7439 3143 Size: 32 1882 32 805 32 1481 32 3529 Fragments arranged by size: 3529 1882 1481 805 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2148 2197 2374 3519 3611 2148 Size: 49 177 1145 92 6362 Fragments arranged by size: 6362 1145 177 92 49 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7825 PsiI TTA'TAA Cuts at: 5395 5395 Size: 7825 PspOMI G'GGCC_C Cuts at: 4327 4749 4327 Size: 422 7403 PstI C_TGCA'G Cuts at: 830 2409 2934 2959 3015 3240 3510 3561 3996 Size: 1579 525 25 56 225 270 51 435 Cuts at: 3996 4365 4392 5019 5105 5177 830 Size: 369 27 627 86 72 3478 Fragments arranged by size: 3478 1579 627 525 435 369 270 225 86 72 56 51 27 25 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7825 PvuII CAG'CTG Cuts at: 2125 3000 3010 4178 4393 4432 2125 Size: 875 10 1168 215 39 5518 Fragments arranged by size: 5518 1168 875 215 39 10 RsrII CG'GwC_CG Cuts at: 3403 3403 Size: 7825 SacI G_AGCT'C Cuts at: 2847 2847 Size: 7825 SacII CC_GC'GG Cuts at: 4662 4662 Size: 7825 SalI G'TCGA_C Cuts at: 1936 5167 1936 Size: 3231 4594 SanDI GG'GwC_CC Cuts at: 2148 2374 3519 3611 2148 Size: 226 1145 92 6362 Fragments arranged by size: 6362 1145 226 92 SapI GCTCTTCn'nnn_ Cuts at: 2810 2852 3230 4527 2810 Size: 42 378 1297 6108 Fragments arranged by size: 6108 1297 378 42 SbfI CC_TGCA'GG Cuts at: 5019 5177 5019 Size: 158 7667 ScaI AGT'ACT Cuts at: 6073 7785 6073 Size: 1712 6113 SfcI C'TryA_G Cuts at: 826 1045 2405 2930 2955 3011 3236 3506 3534 Size: 219 1360 525 25 56 225 270 28 Cuts at: 3534 3557 3992 4361 4388 5015 5101 5173 6308 Size: 23 435 369 27 627 86 72 1135 Cuts at: 6308 6989 7180 826 Size: 681 191 1471 Fragments arranged by size: 1471 1360 1135 681 627 525 435 369 270 225 219 191 86 72 56 28 27 25 23 SfoI GGC'GCC Cuts at: 2264 3795 4112 2264 Size: 1531 317 5977 Fragments arranged by size: 5977 1531 317 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7825 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7825 SmaI CCC'GGG Cuts at: 1872 3394 4055 4216 5152 1872 Size: 1522 661 161 936 4545 Fragments arranged by size: 4545 1522 936 661 161 SmlI C'TyrA_G Cuts at: 820 1017 1943 3335 4304 4789 5929 6830 7092 Size: 197 926 1392 969 485 1140 901 262 Cuts at: 7092 820 Size: 1553 Fragments arranged by size: 1553 1392 1140 969 926 901 485 262 197 SnaBI TAC'GTA Cuts at: 493 5616 493 Size: 5123 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7825 SphI G_CATG'C Cuts at: 1132 3578 4208 4611 5183 1132 Size: 2446 630 403 572 3774 Fragments arranged by size: 3774 2446 630 572 403 SspI AAT'ATT Cuts at: 5 52 5749 5 Size: 47 5697 2081 Fragments arranged by size: 5697 2081 47 StuI AGG'CCT Cuts at: 1517 3956 4118 1517 Size: 2439 162 5224 Fragments arranged by size: 5224 2439 162 StyI C'CwwG_G Cuts at: 513 1065 2004 3601 4444 4590 4770 4842 4996 Size: 552 939 1597 843 146 180 72 154 Cuts at: 4996 5264 513 Size: 268 3074 Fragments arranged by size: 3074 1597 939 843 552 268 180 154 146 72 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2406 2888 3212 4400 4506 4727 4763 Size: 1255 826 482 324 1188 106 221 36 Cuts at: 4763 4820 5852 5869 6022 6207 6652 325 Size: 57 1032 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1188 1032 826 482 445 324 221 185 153 106 57 36 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3312 6071 7783 Size: 274 80 33 51 1443 1335 2759 1712 Cuts at: 7783 96 Size: 138 Fragments arranged by size: 2759 1712 1443 1335 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4148 4180 4656 4688 7785 7817 4148 Size: 32 476 32 3097 32 4156 Fragments arranged by size: 4156 3097 476 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2276 3122 3615 1316 Size: 960 846 493 5526 Fragments arranged by size: 5526 960 846 493 XbaI T'CTAG_A Cuts at: 5077 5161 5077 Size: 84 7741 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2266 2833 2266 Size: 567 7258 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7825 XmaI C'CCGG_G Cuts at: 1870 3392 4053 4214 5150 1870 Size: 1522 661 161 936 4545 Fragments arranged by size: 4545 1522 936 661 161 XmnI GAAnn'nnTTC Cuts at: 3807 5069 5954 3807 Size: 1262 885 5678 Fragments arranged by size: 5678 1262 885 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5633 276 Size: 53 83 186 936 4099 2468 Fragments arranged by size: 4099 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BfrBI BsiWI BstXI BstZ17I EcoRV FalI FseI FspAI NruI NsiI PacI PspXI PsrI SexAI SfiI SrfI SwaI