(Circular) (Six-base) MAPSORT of: pFN21AE0386.seq Check: 9946 from: 1 to: 6787 pFN21AE0386 6787 bp With 182 enzymes: SgfI * April 15, 2010 09:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3057 3057 Size: 6787 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4597 278 Size: 53 83 186 936 3061 2468 Fragments arranged by size: 3061 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2388 2670 4130 1937 Size: 451 282 1460 4594 Fragments arranged by size: 4594 1460 451 282 Acc65I G'GTAC_C Cuts at: 4081 4108 4081 Size: 27 6760 AclI AA'CG_TT Cuts at: 1563 4914 5287 1563 Size: 3351 373 3063 Fragments arranged by size: 3351 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3689 3743 4850 6234 6502 1632 Size: 2057 54 1107 1384 268 1917 Fragments arranged by size: 2057 1917 1384 1107 268 54 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6590 AflIII A'CryG_T Cuts at: 1203 5686 6663 1203 Size: 4483 977 1327 Fragments arranged by size: 4483 1327 977 AgeI A'CCGG_T Cuts at: 5618 5618 Size: 6787 AhdI GACnn_n'nnGTC Cuts at: 5516 5516 Size: 6787 AleI CACnn'nnGTG Cuts at: 1166 2348 2897 1166 Size: 1182 549 5056 Fragments arranged by size: 5056 1182 549 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2366 2987 1184 Size: 1182 621 4984 Fragments arranged by size: 4984 1182 621 AlwNI CAG_nnn'CTG Cuts at: 2876 3531 3613 4530 6102 2876 Size: 655 82 917 1572 3561 Fragments arranged by size: 3561 1572 917 655 82 ApaI G_GGCC'C Cuts at: 2322 2721 3909 2322 Size: 399 1188 5200 Fragments arranged by size: 5200 1188 399 ApaLI G'TGCA_C Cuts at: 3976 4844 6000 3976 Size: 868 1156 4763 Fragments arranged by size: 4763 1156 868 ApoI r'AATT_y Cuts at: 1493 2807 4096 4322 4474 6583 1493 Size: 1314 1289 226 152 2109 1697 Fragments arranged by size: 2109 1697 1314 1289 226 152 AseI AT'TA_AT Cuts at: 160 1032 5341 160 Size: 872 4309 1606 Fragments arranged by size: 4309 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6787 AvaI C'yCGr_G Cuts at: 1870 1943 2750 4112 1870 Size: 73 807 1362 4545 Fragments arranged by size: 4545 1362 807 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2680 2713 2680 Size: 33 6754 BamHI G'GATC_C Cuts at: 3888 4117 4484 3888 Size: 229 367 6191 Fragments arranged by size: 6191 367 229 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2471 2941 4051 4081 4108 Size: 325 217 627 684 470 1110 30 27 Cuts at: 4108 5563 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1110 684 627 470 325 217 30 27 BanII G_rGCy'C Cuts at: 1391 2322 2721 3191 3417 3909 3963 4106 1391 Size: 931 399 470 226 492 54 143 4072 Fragments arranged by size: 4072 931 492 470 399 226 143 54 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2914 3731 928 Size: 971 1015 817 3984 Fragments arranged by size: 3984 1015 971 817 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4978 5012 1903 Size: 34 3041 34 3678 Fragments arranged by size: 3678 3041 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4679 5889 4679 Size: 1210 5577 BclI T'GATC_A Cuts at: 1548 1548 Size: 6787 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3904 5398 136 Size: 107 122 71 631 605 2232 1494 1525 Fragments arranged by size: 2232 1525 1494 631 605 122 107 71 BglII A'GATC_T Cuts at: 6782 6782 Size: 6787 BlpI GC'TnA_GC Cuts at: 4204 4204 Size: 6787 Bme1580I G_kGCm'C Cuts at: 1790 2096 2128 2322 2345 2721 3909 3980 4056 Size: 306 32 194 23 376 1188 71 76 Cuts at: 4056 4848 6004 1790 Size: 792 1156 2573 Fragments arranged by size: 2573 1188 1156 792 376 306 194 76 71 32 23 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6787 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3519 4518 5476 Size: 416 53 83 399 24 2102 999 958 Cuts at: 5476 442 Size: 1753 Fragments arranged by size: 2102 1753 999 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6787 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3398 3436 3398 Size: 38 6749 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2677 2969 3059 3977 5447 1239 Size: 1438 292 90 918 1470 2579 Fragments arranged by size: 2579 1470 1438 918 292 90 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2540 2838 4912 5777 6075 2540 Size: 298 2074 865 298 3252 Fragments arranged by size: 3252 2074 865 298 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2305 2380 5450 882 Size: 617 806 75 3070 2219 Fragments arranged by size: 3070 2219 806 617 75 BsaAI yAC'GTr Cuts at: 493 1204 2247 3445 3688 4578 493 Size: 711 1043 1198 243 890 2702 Fragments arranged by size: 2702 1198 1043 890 711 243 BsaBI GATnn'nnATC Cuts at: 6781 6781 Size: 6787 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2214 4594 4976 5611 Size: 53 83 186 936 681 2380 382 635 Cuts at: 5611 275 Size: 1451 Fragments arranged by size: 2380 1451 936 681 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5220 5618 5892 6039 6450 5220 Size: 398 274 147 411 5557 Fragments arranged by size: 5557 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3091 3121 1178 Size: 30 165 30 1688 30 4844 Fragments arranged by size: 4844 1688 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2738 2861 1186 Size: 1552 123 5112 Fragments arranged by size: 5112 1552 123 BseYI C'CCAG_C Cuts at: 2273 2459 2600 2713 3304 3338 3390 3733 3768 Size: 186 141 113 591 34 52 343 35 Cuts at: 3768 5990 6486 2273 Size: 2222 496 2574 Fragments arranged by size: 2574 2222 591 496 343 186 141 113 52 35 34 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2983 1163 Size: 573 1247 4967 Fragments arranged by size: 4967 1247 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4237 4998 6026 1540 Size: 275 186 2236 761 1028 2301 Fragments arranged by size: 2301 2236 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3417 3980 4848 4933 6004 3417 Size: 563 868 85 1071 4200 Fragments arranged by size: 4200 1071 868 563 85 BsiWI C'GTAC_G Cuts at: 2247 2247 Size: 6787 BsmI GAATG_Cn' Cuts at: 2792 3699 4298 4391 2792 Size: 907 599 93 5188 Fragments arranged by size: 5188 907 599 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2096 2128 2322 2345 2721 3191 3417 Size: 399 306 32 194 23 376 470 226 Cuts at: 3417 3909 3963 3980 4056 4106 4848 4933 6004 Size: 492 54 17 76 50 742 85 1071 Cuts at: 6004 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 492 470 399 376 306 226 194 85 76 54 50 32 23 17 BspEI T'CCGG_A Cuts at: 6450 6450 Size: 6787 BspHI T'CATG_A Cuts at: 4676 6406 4676 Size: 1730 5057 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3057 3385 3932 4142 844 Size: 2213 328 547 210 3489 Fragments arranged by size: 3489 2213 547 328 210 BsrBI CCG'CTC Cuts at: 1394 1823 4674 1394 Size: 429 2851 3507 Fragments arranged by size: 3507 2851 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2407 5282 5456 65 Size: 1183 1159 2875 174 1396 Fragments arranged by size: 2875 1396 1183 1159 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3406 5431 5618 1700 Size: 110 1596 2025 187 2869 Fragments arranged by size: 2869 2025 1596 187 110 BsrGI T'GTAC_A Cuts at: 96 2883 96 Size: 2787 4000 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6787 BssSI C'ACGA_G Cuts at: 4847 5859 4847 Size: 1012 5775 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6787 BstBI TT'CG_AA Cuts at: 5591 5591 Size: 6787 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6787 BstXI CCAn_nnnn'nTGG Cuts at: 2162 3061 2162 Size: 899 5888 BstYI r'GATC_y Cuts at: 1972 2110 2448 3888 4117 4484 4869 4886 6338 Size: 138 338 1440 229 367 385 17 1452 Cuts at: 6338 6424 6436 6782 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1440 385 367 346 338 229 138 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3144 3144 Size: 6787 BtgI C'CryG_G Cuts at: 513 1065 2004 2155 2283 2337 513 Size: 552 939 151 128 54 4963 Fragments arranged by size: 4963 939 552 151 128 54 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2253 487 Size: 683 56 1027 5021 Fragments arranged by size: 5021 1027 683 56 BtsI GCAGTG_nn' Cuts at: 4303 5097 5117 4303 Size: 794 20 5973 Fragments arranged by size: 5973 794 20 ClaI AT'CG_AT Cuts at: 1554 2178 1554 Size: 624 6163 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6752 DraI TTT'AAA Cuts at: 4091 4091 Size: 6787 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2069 5794 809 Size: 507 753 3725 1802 Fragments arranged by size: 3725 1802 753 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2184 3234 3439 Size: 54 1425 50 275 18 354 1050 205 Cuts at: 3439 3516 4234 5123 6752 8 Size: 77 718 889 1629 43 Fragments arranged by size: 1629 1425 1050 889 718 354 275 205 77 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4234 1537 Size: 275 2422 4090 Fragments arranged by size: 4090 2422 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2643 3011 3585 4717 1353 Size: 1290 368 574 1132 3423 Fragments arranged by size: 3423 1290 1132 574 368 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4034 5374 5748 5894 387 Size: 1064 138 2445 1340 374 146 1280 Fragments arranged by size: 2445 1340 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3415 3415 Size: 6787 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2677 2969 3059 3689 3743 3977 4850 Size: 393 1045 292 90 630 54 234 873 Cuts at: 4850 5447 6234 6502 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1045 873 787 630 597 393 292 268 234 90 54 EcoNI CCTnn'n_nnAGG Cuts at: 2843 3832 4010 2843 Size: 989 178 5620 Fragments arranged by size: 5620 989 178 EcoO109I rG'GnC_Cy Cuts at: 2456 2597 2717 3049 3825 3906 3921 2456 Size: 141 120 332 776 81 15 5322 Fragments arranged by size: 5322 776 332 141 120 81 15 EcoRI G'AATT_C Cuts at: 2807 4096 4474 2807 Size: 1289 378 5120 Fragments arranged by size: 5120 1289 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3844 3997 3844 Size: 153 6634 FspI TGC'GCA Cuts at: 5293 5293 Size: 6787 HaeII r_GCGC'y Cuts at: 1678 3407 5934 1678 Size: 1729 2527 2531 Fragments arranged by size: 2531 2527 1729 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2285 2317 2440 2472 5428 Size: 32 135 32 713 32 123 32 2956 Cuts at: 5428 5460 5502 5534 1373 Size: 32 42 32 2626 Fragments arranged by size: 2956 2626 713 135 123 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4131 4377 669 Size: 988 281 2193 246 3079 Fragments arranged by size: 3079 2193 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6787 HpaI GTT'AAC Cuts at: 4377 4377 Size: 6787 KpnI G_GTAC'C Cuts at: 4085 4112 4085 Size: 27 6760 MfeI C'AATT_G Cuts at: 4386 4386 Size: 6787 MluI A'CGCG_T Cuts at: 6663 6663 Size: 6787 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3997 5901 6085 1305 Size: 2692 1904 184 2007 Fragments arranged by size: 2692 2007 1904 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2186 3236 3441 3518 10 Size: 54 1425 343 354 1050 205 77 3279 Fragments arranged by size: 3279 1425 1050 354 343 205 77 54 MslI CAynn'nnrTG Cuts at: 518 1166 2348 2897 3447 4745 5104 5263 518 Size: 648 1182 549 550 1298 359 159 2042 Fragments arranged by size: 2042 1298 1182 648 550 549 359 159 MspA1I CmG'CkG Cuts at: 1606 2118 2348 2463 2604 2977 3304 3772 4203 Size: 512 230 115 141 373 327 468 431 Cuts at: 4203 4880 6028 6273 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 512 468 431 373 327 245 230 141 115 NcoI C'CATG_G Cuts at: 513 1065 2004 2155 513 Size: 552 939 151 5145 Fragments arranged by size: 5145 939 552 151 NdeI CA'TA_TG Cuts at: 387 387 Size: 6787 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6787 NotI GC'GGCC_GC Cuts at: 4234 4234 Size: 6787 NspI r_CATG'y Cuts at: 1132 1229 4145 5690 1132 Size: 97 2916 1545 2229 Fragments arranged by size: 2916 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3964 1348 Size: 2616 4171 PciI A'CATG_T Cuts at: 5686 5686 Size: 6787 PflMI CCAn_nnn'nTGG Cuts at: 1108 2223 2226 2466 3232 3652 1108 Size: 1115 3 240 766 420 4243 Fragments arranged by size: 4243 1115 766 420 240 3 PfoI T'CCnGG_A Cuts at: 3075 3210 3075 Size: 135 6652 PmeI GTTT'AAAC Cuts at: 4091 4091 Size: 6787 PmlI CAC'GTG Cuts at: 3445 3445 Size: 6787 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4856 4888 6369 6401 4856 Size: 32 1481 32 5242 Fragments arranged by size: 5242 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2456 3049 3825 3921 2456 Size: 593 776 96 5322 Fragments arranged by size: 5322 776 593 96 PshAI GACnn'nnGTC Cuts at: 1613 4013 1613 Size: 2400 4387 PsiI TTA'TAA Cuts at: 4357 4357 Size: 6787 PspOMI G'GGCC_C Cuts at: 2318 2717 3905 2318 Size: 399 1188 5200 Fragments arranged by size: 5200 1188 399 PstI C_TGCA'G Cuts at: 830 2976 2982 4139 830 Size: 2146 6 1157 3478 Fragments arranged by size: 3478 2146 1157 6 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6787 PvuII CAG'CTG Cuts at: 2348 2604 2977 3772 2348 Size: 256 373 795 5363 Fragments arranged by size: 5363 795 373 256 SacI G_AGCT'C Cuts at: 3417 3417 Size: 6787 SalI G'TCGA_C Cuts at: 1936 4129 1936 Size: 2193 4594 SanDI GG'GwC_CC Cuts at: 2456 2456 Size: 6787 SapI GCTCTTCn'nnn_ Cuts at: 3011 3585 3011 Size: 574 6213 SbfI CC_TGCA'GG Cuts at: 4139 4139 Size: 6787 ScaI AGT'ACT Cuts at: 3179 5035 6747 3179 Size: 1856 1712 3219 Fragments arranged by size: 3219 1856 1712 SfcI C'TryA_G Cuts at: 826 1045 2787 2972 2978 4135 5270 5951 6142 Size: 219 1742 185 6 1157 1135 681 191 Cuts at: 6142 826 Size: 1471 Fragments arranged by size: 1742 1471 1157 1135 681 219 191 185 6 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6787 SgrAI Cr'CCGG_yG Cuts at: 1700 3406 1700 Size: 1706 5081 SmaI CCC'GGG Cuts at: 1872 4114 1872 Size: 2242 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2519 2853 4891 5792 6054 820 Size: 197 926 576 334 2038 901 262 1553 Fragments arranged by size: 2038 1553 926 901 576 334 262 197 SnaBI TAC'GTA Cuts at: 493 4578 493 Size: 4085 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6787 SphI G_CATG'C Cuts at: 1132 4145 1132 Size: 3013 3774 SspI AAT'ATT Cuts at: 5 52 4711 5 Size: 47 4659 2081 Fragments arranged by size: 4659 2081 47 StuI AGG'CCT Cuts at: 1517 2817 1517 Size: 1300 5487 StyI C'CwwG_G Cuts at: 513 1065 2004 2155 2593 2643 2739 3286 4226 Size: 552 939 151 438 50 96 547 940 Cuts at: 4226 513 Size: 3074 Fragments arranged by size: 3074 940 939 552 547 438 151 96 50 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2293 3116 4814 4831 4984 5169 5614 Size: 1255 713 823 1698 17 153 185 445 Cuts at: 5614 325 Size: 1498 Fragments arranged by size: 1698 1498 1255 823 713 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2883 3177 5033 Size: 274 80 33 51 1443 906 294 1856 Cuts at: 5033 6745 96 Size: 1712 138 Fragments arranged by size: 1856 1712 1443 906 294 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3091 3123 6747 6779 3091 Size: 32 3624 32 3099 Fragments arranged by size: 3624 3099 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3121 1316 Size: 1805 4982 XbaI T'CTAG_A Cuts at: 2380 4123 2380 Size: 1743 5044 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2281 2011 Size: 270 6517 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6787 XmaI C'CCGG_G Cuts at: 1870 4112 1870 Size: 2242 4545 XmnI GAAnn'nnTTC Cuts at: 4916 4916 Size: 6787 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4595 276 Size: 53 83 186 936 3061 2468 Fragments arranged by size: 3061 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbeI BbvCI BfrBI Bpu10I BsmBI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI