Restriction map for
pFN21AE0386
(Linear) (Six-base) MAPSORT of: KIEE0386.seq Check: 9876 from: 1 to: 2082
KIEE0386.orf
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1052 2082
Size: 1052 1030
AccI GT'mk_AC
Cuts at: 0 383 665 2082
Size: 383 282 1417
Fragments arranged by size:
1417 383 282
Acc65I G'GTAC_C
Cuts at: 0 2076 2082
Size: 2076 6
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1684 1738 2082
Size: 1684 54 344
Fragments arranged by size:
1684 344 54
AleI CACnn'nnGTG
Cuts at: 0 343 892 2082
Size: 343 549 1190
Fragments arranged by size:
1190 549 343
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 361 982 2082
Size: 361 621 1100
Fragments arranged by size:
1100 621 361
AlwNI CAG_nnn'CTG
Cuts at: 0 871 1526 1608 2082
Size: 871 655 82 474
Fragments arranged by size:
871 655 474 82
ApaI G_GGCC'C
Cuts at: 0 317 716 1904 2082
Size: 317 399 1188 178
Fragments arranged by size:
1188 399 317 178
ApaLI G'TGCA_C
Cuts at: 0 1971 2082
Size: 1971 111
ApoI r'AATT_y
Cuts at: 0 802 2082
Size: 802 1280
AvaI C'yCGr_G
Cuts at: 0 745 2082
Size: 745 1337
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 675 708 2082
Size: 675 33 1374
Fragments arranged by size:
1374 675 33
BamHI G'GATC_C
Cuts at: 0 1883 2082
Size: 1883 199
BanI G'GyrC_C
Cuts at: 0 466 936 2046 2076 2082
Size: 466 470 1110 30 6
Fragments arranged by size:
1110 470 466 30 6
BanII G_rGCy'C
Cuts at: 0 317 716 1186 1412 1904 1958 2082
Size: 317 399 470 226 492 54 124
Fragments arranged by size:
492 470 399 317 226 124 54
BbsI GAAGACnn'nnnn_
Cuts at: 0 909 1726 2082
Size: 909 817 356
Fragments arranged by size:
909 817 356
BglI GCCn_nnn'nGGC
Cuts at: 0 1899 2082
Size: 1899 183
Bme1580I G_kGCm'C
Cuts at: 0 91 123 317 340 716 1904 1975 2051
Size: 91 32 194 23 376 1188 71 76
Cuts at: 2051 2082
Size: 31
Fragments arranged by size:
1188 376 194 91 76 71 32 31
23
BmrI ACTGGGnnnn_n'
Cuts at: 0 1514 2082
Size: 1514 568
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1393 1431 2082
Size: 1393 38 651
Fragments arranged by size:
1393 651 38
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 672 964 1054 1972 2082
Size: 672 292 90 918 110
Fragments arranged by size:
918 672 292 110 90
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 535 833 2082
Size: 535 298 1249
Fragments arranged by size:
1249 535 298
BsaI GGTCTCn'nnnn_
Cuts at: 0 300 375 2082
Size: 300 75 1707
Fragments arranged by size:
1707 300 75
BsaAI yAC'GTr
Cuts at: 0 242 1440 1683 2082
Size: 242 1198 243 399
Fragments arranged by size:
1198 399 243 242
BsaHI Gr'CG_yC
Cuts at: 0 209 2082
Size: 209 1873
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1086 1116 2082
Size: 1086 30 966
Fragments arranged by size:
1086 966 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 733 856 2082
Size: 733 123 1226
Fragments arranged by size:
1226 733 123
BseYI C'CCAG_C
Cuts at: 0 268 454 595 708 1299 1333 1385 1728
Size: 268 186 141 113 591 34 52 343
Cuts at: 1728 1763 2082
Size: 35 319
Fragments arranged by size:
591 343 319 268 186 141 113 52
35 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 978 2082
Size: 978 1104
BsiHKAI G_wGCw'C
Cuts at: 0 1412 1975 2082
Size: 1412 563 107
Fragments arranged by size:
1412 563 107
BsiWI C'GTAC_G
Cuts at: 0 242 2082
Size: 242 1840
BsmI GAATG_Cn'
Cuts at: 0 787 1694 2082
Size: 787 907 388
Fragments arranged by size:
907 787 388
Bsp1286I G_dGCh'C
Cuts at: 0 91 123 317 340 716 1186 1412 1904
Size: 91 32 194 23 376 470 226 492
Cuts at: 1904 1958 1975 2051 2082
Size: 54 17 76 31
Fragments arranged by size:
492 470 376 226 194 91 76 54
32 31 23 17
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1052 1380 1927 2082
Size: 1052 328 547 155
Fragments arranged by size:
1052 547 328 155
BsrDI GCAATG_nn'
Cuts at: 0 402 2082
Size: 402 1680
BsrFI r'CCGG_y
Cuts at: 0 1401 2082
Size: 1401 681
BsrGI T'GTAC_A
Cuts at: 0 878 2082
Size: 878 1204
BstXI CCAn_nnnn'nTGG
Cuts at: 0 157 1056 2082
Size: 157 899 1026
Fragments arranged by size:
1026 899 157
BstYI r'GATC_y
Cuts at: 0 105 443 1883 2082
Size: 105 338 1440 199
Fragments arranged by size:
1440 338 199 105
Bsu36I CC'TnA_GG
Cuts at: 0 1139 2082
Size: 1139 943
BtgI C'CryG_G
Cuts at: 0 150 278 332 2082
Size: 150 128 54 1750
Fragments arranged by size:
1750 150 128 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 248 2082
Size: 248 1834
ClaI AT'CG_AT
Cuts at: 0 173 2082
Size: 173 1909
DrdI GACnn_nn'nnGTC
Cuts at: 0 64 2082
Size: 64 2018
EaeI y'GGCC_r
Cuts at: 0 179 1229 1434 1511 2082
Size: 179 1050 205 77 571
Fragments arranged by size:
1050 571 205 179 77
EarI CTCTTCn'nnn_
Cuts at: 0 638 1006 1580 2082
Size: 638 368 574 502
Fragments arranged by size:
638 574 502 368
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2029 2082
Size: 2029 53
EcoICRI GAG'CTC
Cuts at: 0 1410 2082
Size: 1410 672
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 672 964 1054 1684 1738 1972 2082
Size: 672 292 90 630 54 234 110
Fragments arranged by size:
672 630 292 234 110 90 54
EcoNI CCTnn'n_nnAGG
Cuts at: 0 838 1827 2005 2082
Size: 838 989 178 77
Fragments arranged by size:
989 838 178 77
EcoO109I rG'GnC_Cy
Cuts at: 0 451 592 712 1044 1820 1901 1916 2082
Size: 451 141 120 332 776 81 15 166
Fragments arranged by size:
776 451 332 166 141 120 81 15
EcoRI G'AATT_C
Cuts at: 0 802 2082
Size: 802 1280
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1839 1992 2082
Size: 1839 153 90
Fragments arranged by size:
1839 153 90
HaeII r_GCGC'y
Cuts at: 0 1402 2082
Size: 1402 680
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 280 312 435 467 2082
Size: 280 32 123 32 1615
Fragments arranged by size:
1615 280 123 32 32
KpnI G_GTAC'C
Cuts at: 0 2080 2082
Size: 2080 2
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1992 2082
Size: 1992 90
MscI TGG'CCA
Cuts at: 0 181 1231 1436 1513 2082
Size: 181 1050 205 77 569
Fragments arranged by size:
1050 569 205 181 77
MslI CAynn'nnrTG
Cuts at: 0 343 892 1442 2082
Size: 343 549 550 640
Fragments arranged by size:
640 550 549 343
MspA1I CmG'CkG
Cuts at: 0 113 343 458 599 972 1299 1767 2082
Size: 113 230 115 141 373 327 468 315
Fragments arranged by size:
468 373 327 315 230 141 115 113
NcoI C'CATG_G
Cuts at: 0 150 2082
Size: 150 1932
PasI CC'CwG_GG
Cuts at: 0 1959 2082
Size: 1959 123
PflMI CCAn_nnn'nTGG
Cuts at: 0 218 221 461 1227 1647 2082
Size: 218 3 240 766 420 435
Fragments arranged by size:
766 435 420 240 218 3
PfoI T'CCnGG_A
Cuts at: 0 1070 1205 2082
Size: 1070 135 877
Fragments arranged by size:
1070 877 135
PmlI CAC'GTG
Cuts at: 0 1440 2082
Size: 1440 642
PpuMI rG'GwC_Cy
Cuts at: 0 451 1044 1820 1916 2082
Size: 451 593 776 96 166
Fragments arranged by size:
776 593 451 166 96
PshAI GACnn'nnGTC
Cuts at: 0 2008 2082
Size: 2008 74
PspOMI G'GGCC_C
Cuts at: 0 313 712 1900 2082
Size: 313 399 1188 182
Fragments arranged by size:
1188 399 313 182
PstI C_TGCA'G
Cuts at: 0 971 977 2082
Size: 971 6 1105
Fragments arranged by size:
1105 971 6
PvuII CAG'CTG
Cuts at: 0 343 599 972 1767 2082
Size: 343 256 373 795 315
Fragments arranged by size:
795 373 343 315 256
SacI G_AGCT'C
Cuts at: 0 1412 2082
Size: 1412 670
SanDI GG'GwC_CC
Cuts at: 0 451 2082
Size: 451 1631
SapI GCTCTTCn'nnn_
Cuts at: 0 1006 1580 2082
Size: 1006 574 502
Fragments arranged by size:
1006 574 502
ScaI AGT'ACT
Cuts at: 0 1174 2082
Size: 1174 908
SfcI C'TryA_G
Cuts at: 0 782 967 973 2082
Size: 782 185 6 1109
Fragments arranged by size:
1109 782 185 6
SgrAI Cr'CCGG_yG
Cuts at: 0 1401 2082
Size: 1401 681
SmlI C'TyrA_G
Cuts at: 0 514 848 2082
Size: 514 334 1234
Fragments arranged by size:
1234 514 334
StuI AGG'CCT
Cuts at: 0 812 2082
Size: 812 1270
StyI C'CwwG_G
Cuts at: 0 150 588 638 734 1281 2082
Size: 150 438 50 96 547 801
Fragments arranged by size:
801 547 438 150 96 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 288 1111 2082
Size: 288 823 971
Fragments arranged by size:
971 823 288
TatI w'GTAC_w
Cuts at: 0 878 1172 2082
Size: 878 294 910
Fragments arranged by size:
910 878 294
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1086 1118 2082
Size: 1086 32 964
Fragments arranged by size:
1086 964 32
Tth111I GACn'n_nGTC
Cuts at: 0 1116 2082
Size: 1116 966
XbaI T'CTAG_A
Cuts at: 0 375 2082
Size: 375 1707
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 276 2082
Size: 276 1806
Enzymes that do cut:
AarI AccI Acc65I AcuI AleI AlfI AlwNI ApaI
ApaLI ApoI AvaI BaeI BamHI BanI BanII BbsI
BglI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsiWI BsmI
Bsp1286I BspMI BsrDI BsrFI BsrGI BstXI BstYI Bsu36I
BtgI BtgZI ClaI DrdI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I KpnI
MmeI MscI MslI MspA1I NcoI PasI PflMI PfoI
PmlI PpuMI PshAI PspOMI PstI PvuII SacI SanDI
SapI ScaI SfcI SgrAI SmlI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI
Enzymes that do not cut:
AatII AclI AfeI AflII AflIII AgeI AhdI AloI
AscI AseI AsiSI AvrII BbeI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI BmgBI BmtI Bpu10I BsaBI
BsaWI BsiEI BsmBI BspEI BspHI BsrBI BssHII BssSI
BstAPI BstBI BstEII BstZ17I BtsI CspCI DraI DraIII
EagI EcoRV FseI FspI FspAI HincII HindIII HpaI
KasI MfeI MluI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PciI PmeI PpiI
PsiI PspXI PsrI PvuI RsrII SacII SalI SbfI
SexAI SfiI SfoI SgfI SmaI SnaBI SpeI SphI
SrfI SspI SwaI XhoI XmaI XmnI ZraI