Restriction map for
pFN21AB8477
(Linear) (Six-base) MAPSORT of: KIBB8477.seq Check: 2475 from: 1 to: 2742
KIBB8477.orf
With 182 enzymes: SgfI *
July 15, 2010 18:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 941 1936 2742
Size: 941 995 806
Fragments arranged by size:
995 941 806
AccI GT'mk_AC
Cuts at: 0 173 875 1476 2742
Size: 173 702 601 1266
Fragments arranged by size:
1266 702 601 173
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 137 284 566 821 865 1321 1532 1925
Size: 137 147 282 255 44 456 211 393
Cuts at: 1925 2187 2533 2742
Size: 262 346 209
Fragments arranged by size:
456 393 346 282 262 255 211 209
147 137 44
AflIII A'CryG_T
Cuts at: 0 900 2742
Size: 900 1842
AhdI GACnn_n'nnGTC
Cuts at: 0 1768 2742
Size: 1768 974
AleI CACnn'nnGTG
Cuts at: 0 2125 2742
Size: 2125 617
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1523 1742 2742
Size: 1523 219 1000
Fragments arranged by size:
1523 1000 219
AlwNI CAG_nnn'CTG
Cuts at: 0 712 752 1539 2608 2742
Size: 712 40 787 1069 134
Fragments arranged by size:
1069 787 712 134 40
ApaLI G'TGCA_C
Cuts at: 0 1995 2742
Size: 1995 747
ApoI r'AATT_y
Cuts at: 0 2130 2334 2400 2622 2742
Size: 2130 204 66 222 120
Fragments arranged by size:
2130 222 204 120 66
AvaI C'yCGr_G
Cuts at: 0 1798 2381 2742
Size: 1798 583 361
Fragments arranged by size:
1798 583 361
BanI G'GyrC_C
Cuts at: 0 2551 2742
Size: 2551 191
BanII G_rGCy'C
Cuts at: 0 1402 2742
Size: 1402 1340
BbsI GAAGACnn'nnnn_
Cuts at: 0 1327 2659 2742
Size: 1327 1332 83
Fragments arranged by size:
1332 1327 83
BbvCI CC'TCA_GC
Cuts at: 0 1630 2742
Size: 1630 1112
BclI T'GATC_A
Cuts at: 0 474 2742
Size: 474 2268
BfrBI ATG'CAT
Cuts at: 0 235 2742
Size: 235 2507
BglII A'GATC_T
Cuts at: 0 1057 2742
Size: 1057 1685
Bme1580I G_kGCm'C
Cuts at: 0 1999 2556 2742
Size: 1999 557 186
Fragments arranged by size:
1999 557 186
BmrI ACTGGGnnnn_n'
Cuts at: 0 1118 1605 1785 2530 2692 2742
Size: 1118 487 180 745 162 50
Fragments arranged by size:
1118 745 487 180 162 50
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 870 2407 2742
Size: 870 1537 335
Fragments arranged by size:
1537 870 335
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 236 469 2275 2419 2742
Size: 236 233 1806 144 323
Fragments arranged by size:
1806 323 236 233 144
Bpu10I CC'TnA_GC
Cuts at: 0 1465 1630 2742
Size: 1465 165 1112
Fragments arranged by size:
1465 1112 165
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 596 634 2472 2742
Size: 596 38 1838 270
Fragments arranged by size:
1838 596 270 38
BsaI GGTCTCn'nnnn_
Cuts at: 0 2448 2742
Size: 2448 294
BsaBI GATnn'nnATC
Cuts at: 0 371 2742
Size: 371 2371
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 198 228 2742
Size: 198 30 2514
Fragments arranged by size:
2514 198 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 466 1399 1858 2742
Size: 466 933 459 884
Fragments arranged by size:
933 884 466 459
BseYI C'CCAG_C
Cuts at: 0 1263 2065 2742
Size: 1263 802 677
Fragments arranged by size:
1263 802 677
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 961 1587 2742
Size: 961 626 1155
Fragments arranged by size:
1155 961 626
BsiHKAI G_wGCw'C
Cuts at: 0 1402 1999 2742
Size: 1402 597 743
Fragments arranged by size:
1402 743 597
BsmI GAATG_Cn'
Cuts at: 0 21 2742
Size: 21 2721
BsmBI CGTCTCn'nnnn_
Cuts at: 0 537 2742
Size: 537 2205
Bsp1286I G_dGCh'C
Cuts at: 0 1402 1999 2556 2742
Size: 1402 597 557 186
Fragments arranged by size:
1402 597 557 186
BspHI T'CATG_A
Cuts at: 0 53 1307 2742
Size: 53 1254 1435
Fragments arranged by size:
1435 1254 53
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 941 1936 2742
Size: 941 995 806
Fragments arranged by size:
995 941 806
BsrBI CCG'CTC
Cuts at: 0 1250 2742
Size: 1250 1492
BsrDI GCAATG_nn'
Cuts at: 0 536 2742
Size: 536 2206
BssSI C'ACGA_G
Cuts at: 0 979 2742
Size: 979 1763
BstAPI GCAn_nnn'nTGC
Cuts at: 0 241 2742
Size: 241 2501
BstBI TT'CG_AA
Cuts at: 0 525 2742
Size: 525 2217
BstEII G'GTnAC_C
Cuts at: 0 2058 2742
Size: 2058 684
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1096 2220 2563 2742
Size: 1096 1124 343 179
Fragments arranged by size:
1124 1096 343 179
BstYI r'GATC_y
Cuts at: 0 108 1057 1529 1919 2742
Size: 108 949 472 390 823
Fragments arranged by size:
949 823 472 390 108
Bsu36I CC'TnA_GG
Cuts at: 0 1428 2742
Size: 1428 1314
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1444 2742
Size: 1444 1298
BtsI GCAGTG_nn'
Cuts at: 0 1453 1641 2742
Size: 1453 188 1101
Fragments arranged by size:
1453 1101 188
DraI TTT'AAA
Cuts at: 0 1185 2621 2742
Size: 1185 1436 121
Fragments arranged by size:
1436 1185 121
EaeI y'GGCC_r
Cuts at: 0 2222 2481 2742
Size: 2222 259 261
Fragments arranged by size:
2222 261 259
EarI CTCTTCn'nnn_
Cuts at: 0 260 587 659 683 811 839 1826 1835
Size: 260 327 72 24 128 28 987 9
Cuts at: 1835 1880 2509 2742
Size: 45 629 233
Fragments arranged by size:
987 629 327 260 233 128 72 45
28 24 9
EcoICRI GAG'CTC
Cuts at: 0 1400 2742
Size: 1400 1342
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 137 236 284 469 566 821 865 1321
Size: 137 99 48 185 97 255 44 456
Cuts at: 1321 1532 1925 2187 2275 2419 2533 2742
Size: 211 393 262 88 144 114 209
Fragments arranged by size:
456 393 262 255 211 209 185 144
137 114 99 97 88 48 44
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1366 2734 2742
Size: 1366 1368 8
Fragments arranged by size:
1368 1366 8
EcoO109I rG'GnC_Cy
Cuts at: 0 1423 2742
Size: 1423 1319
EcoRI G'AATT_C
Cuts at: 0 2130 2742
Size: 2130 612
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 865 1521 1909 2514 2742
Size: 865 656 388 605 228
Fragments arranged by size:
865 656 605 388 228
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 82 114 198 230 232 264 389 421
Size: 82 32 84 32 2 32 125 32
Cuts at: 421 1156 1188 1339 1371 1754 1786 2742
Size: 735 32 151 32 383 32 956
Fragments arranged by size:
956 735 383 151 125 84 82 32
32 32 32 32 32 32 2
HincII GTy'rAC
Cuts at: 0 1477 2742
Size: 1477 1265
HindIII A'AGCT_T
Cuts at: 0 938 2742
Size: 938 1804
MfeI C'AATT_G
Cuts at: 0 457 2742
Size: 457 2285
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 608 1313 2141 2742
Size: 608 705 828 601
Fragments arranged by size:
828 705 608 601
MscI TGG'CCA
Cuts at: 0 2224 2483 2742
Size: 2224 259 259
Fragments arranged by size:
2224 259 259
MslI CAynn'nnrTG
Cuts at: 0 337 2125 2742
Size: 337 1788 617
Fragments arranged by size:
1788 617 337
MspA1I CmG'CkG
Cuts at: 0 225 489 569 1126 1524 2422 2742
Size: 225 264 80 557 398 898 320
Fragments arranged by size:
898 557 398 320 264 225 80
NdeI CA'TA_TG
Cuts at: 0 1095 2742
Size: 1095 1647
NsiI A_TGCA'T
Cuts at: 0 237 2742
Size: 237 2505
NspI r_CATG'y
Cuts at: 0 235 904 2742
Size: 235 669 1838
Fragments arranged by size:
1838 669 235
PciI A'CATG_T
Cuts at: 0 900 2742
Size: 900 1842
PflMI CCAn_nnn'nTGG
Cuts at: 0 1410 1539 2742
Size: 1410 129 1203
Fragments arranged by size:
1410 1203 129
PfoI T'CCnGG_A
Cuts at: 0 2435 2461 2742
Size: 2435 26 281
Fragments arranged by size:
2435 281 26
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1486 1518 2742
Size: 1486 32 1224
Fragments arranged by size:
1486 1224 32
PpuMI rG'GwC_Cy
Cuts at: 0 1423 2742
Size: 1423 1319
PspXI vC'TCGA_Gb
Cuts at: 0 2381 2742
Size: 2381 361
PstI C_TGCA'G
Cuts at: 0 494 626 2742
Size: 494 132 2116
Fragments arranged by size:
2116 494 132
PvuII CAG'CTG
Cuts at: 0 489 569 1126 2422 2742
Size: 489 80 557 1296 320
Fragments arranged by size:
1296 557 489 320 80
SacI G_AGCT'C
Cuts at: 0 1402 2742
Size: 1402 1340
SalI G'TCGA_C
Cuts at: 0 1475 2742
Size: 1475 1267
SapI GCTCTTCn'nnn_
Cuts at: 0 683 839 2742
Size: 683 156 1903
Fragments arranged by size:
1903 683 156
SexAI A'CCwGG_T
Cuts at: 0 1764 2742
Size: 1764 978
SfcI C'TryA_G
Cuts at: 0 490 622 1448 2025 2148 2742
Size: 490 132 826 577 123 594
Fragments arranged by size:
826 594 577 490 132 123
SmaI CCC'GGG
Cuts at: 0 1800 2742
Size: 1800 942
SmlI C'TyrA_G
Cuts at: 0 611 649 2381 2451 2742
Size: 611 38 1732 70 291
Fragments arranged by size:
1732 611 291 70 38
SphI G_CATG'C
Cuts at: 0 235 2742
Size: 235 2507
SspI AAT'ATT
Cuts at: 0 2033 2742
Size: 2033 709
StyI C'CwwG_G
Cuts at: 0 686 2742
Size: 686 2056
SwaI ATTT'AAAT
Cuts at: 0 2621 2742
Size: 2621 121
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1624 1947 2713 2730 2742
Size: 1624 323 766 17 12
Fragments arranged by size:
1624 766 323 17 12
Tth111I GACn'n_nGTC
Cuts at: 0 210 2742
Size: 210 2532
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2443 2742
Size: 2443 299
XhoI C'TCGA_G
Cuts at: 0 2381 2742
Size: 2381 361
XmaI C'CCGG_G
Cuts at: 0 1798 2742
Size: 1798 944
XmnI GAAnn'nnTTC
Cuts at: 0 1501 2742
Size: 1501 1241
Enzymes that do cut:
AarI AccI AcuI AflIII AhdI AleI AlfI AlwNI
ApaLI ApoI AvaI BanI BanII BbsI BbvCI BclI
BfrBI BglII Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaBI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgZI BtsI DraI
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
Hin4I HincII HindIII MfeI MmeI MscI MslI MspA1I
NdeI NsiI NspI PciI PflMI PfoI PpiI PpuMI
PspXI PstI PvuII SacI SalI SapI SexAI SfcI
SmaI SmlI SphI SspI StyI SwaI TaqII Tth111I
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AloI ApaI
AscI AseI AsiSI AvrII BaeI BamHI BbeI BcgI
BciVI BglI BlpI BmgBI BmtI BsaAI BsaHI BsaWI
BsiEI BsiWI BspEI BsrFI BsrGI BssHII BstZ17I BtgI
ClaI CspCI DraIII DrdI EagI EciI EcoRV FseI
FspI FspAI HaeII HpaI KasI KpnI MluI NaeI
NarI NcoI NgoMIV NheI NotI NruI PacI PasI
PmeI PmlI PshAI PsiI PspOMI PsrI PvuI RsrII
SacII SanDI SbfI ScaI SfiI SfoI SgfI SgrAI
SnaBI SpeI SrfI StuI TatI TstI XbaI ZraI