(Circular) (Six-base) MAPSORT of: pFN21AB8522.seq Check: 2943 from: 1 to: 7342 pFN21AB8522 7342 bp With 182 enzymes: SgfI * August 16, 2021 15:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5152 278 Size: 53 83 186 936 3616 2468 Fragments arranged by size: 3616 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4154 4685 1937 Size: 2217 531 4594 Fragments arranged by size: 4594 2217 531 Acc65I G'GTAC_C Cuts at: 4663 4663 Size: 7342 AclI AA'CG_TT Cuts at: 1563 5469 5842 1563 Size: 3906 373 3063 Fragments arranged by size: 3906 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2187 5405 6789 7057 1632 Size: 555 3218 1384 268 1917 Fragments arranged by size: 3218 1917 1384 555 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7145 AflIII A'CryG_T Cuts at: 1203 2297 2640 3405 6241 7218 1203 Size: 1094 343 765 2836 977 1327 Fragments arranged by size: 2836 1327 1094 977 765 343 AgeI A'CCGG_T Cuts at: 6173 6173 Size: 7342 AhdI GACnn_n'nnGTC Cuts at: 6071 6071 Size: 7342 AleI CACnn'nnGTG Cuts at: 1166 2894 4586 1166 Size: 1728 1692 3922 Fragments arranged by size: 3922 1728 1692 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4604 1184 Size: 3420 3922 AlwNI CAG_nnn'CTG Cuts at: 5085 6657 5085 Size: 1572 5770 ApaLI G'TGCA_C Cuts at: 5399 6555 5399 Size: 1156 6186 ApoI r'AATT_y Cuts at: 1493 4046 4294 4651 4877 5029 7138 1493 Size: 2553 248 357 226 152 2109 1697 Fragments arranged by size: 2553 2109 1697 357 248 226 152 AseI AT'TA_AT Cuts at: 160 1032 5896 160 Size: 872 4864 1606 Fragments arranged by size: 4864 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7342 AvaI C'yCGr_G Cuts at: 1870 1943 3941 4667 1870 Size: 73 1998 726 4545 Fragments arranged by size: 4545 1998 726 73 BamHI G'GATC_C Cuts at: 2393 4672 5039 2393 Size: 2279 367 4696 Fragments arranged by size: 4696 2279 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2960 4142 4663 6118 618 Size: 325 217 627 1173 1182 521 1455 1842 Fragments arranged by size: 1842 1455 1182 1173 627 521 325 217 BanII G_rGCy'C Cuts at: 1391 2856 4661 1391 Size: 1465 1805 4072 Fragments arranged by size: 4072 1805 1465 BbeI G_GCGC'C Cuts at: 2964 2964 Size: 7342 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2196 3729 928 Size: 971 297 1533 4541 Fragments arranged by size: 4541 1533 971 297 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3524 3558 3828 3862 5533 5567 1903 Size: 34 1587 34 270 34 1671 34 3678 Fragments arranged by size: 3678 1671 1587 270 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2287 2302 5234 6444 2287 Size: 15 2932 1210 3185 Fragments arranged by size: 3185 2932 1210 15 BclI T'GATC_A Cuts at: 1548 1548 Size: 7342 BfrBI ATG'CAT Cuts at: 2318 3780 2318 Size: 1462 5880 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4434 5953 136 Size: 107 122 71 631 605 2762 1519 1525 Fragments arranged by size: 2762 1525 1519 631 605 122 107 71 BglII A'GATC_T Cuts at: 2613 7337 2613 Size: 4724 2618 BlpI GC'TnA_GC Cuts at: 4759 4759 Size: 7342 Bme1580I G_kGCm'C Cuts at: 1790 2580 3547 4289 5403 6559 1790 Size: 790 967 742 1114 1156 2573 Fragments arranged by size: 2573 1156 1114 967 790 742 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7342 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3469 3520 3737 Size: 416 53 83 399 24 2052 51 217 Cuts at: 3737 4053 4560 5073 6031 442 Size: 316 507 513 958 1753 Fragments arranged by size: 2052 1753 958 513 507 416 399 316 217 83 53 51 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7342 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2998 2998 Size: 7342 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2354 2993 3077 4151 6002 1239 Size: 1115 639 84 1074 1851 2579 Fragments arranged by size: 2579 1851 1115 1074 639 84 Bpu10I CC'TnA_GC Cuts at: 2038 2038 Size: 7342 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5467 6332 6630 5467 Size: 865 298 6179 Fragments arranged by size: 6179 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6005 882 Size: 617 4506 2219 Fragments arranged by size: 4506 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2641 4338 4584 5133 493 Size: 711 1437 1697 246 549 2702 Fragments arranged by size: 2702 1697 1437 711 549 246 BsaBI GATnn'nnATC Cuts at: 3596 7336 3596 Size: 3740 3602 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2961 3726 5149 5531 Size: 53 83 186 936 1428 765 1423 382 Cuts at: 5531 6166 275 Size: 635 1451 Fragments arranged by size: 1451 1428 1423 936 765 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5775 6173 6447 6594 7005 5775 Size: 398 274 147 411 6112 Fragments arranged by size: 6112 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3062 3092 1178 Size: 30 165 30 1659 30 5428 Fragments arranged by size: 5428 1659 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 7342 BseYI C'CCAG_C Cuts at: 2910 2985 3946 6545 7041 2910 Size: 75 961 2599 496 3211 Fragments arranged by size: 3211 2599 961 496 75 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3106 3550 4418 4505 1163 Size: 573 1370 444 868 87 4000 Fragments arranged by size: 4000 1370 868 573 444 87 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3007 4792 5553 6581 1540 Size: 275 186 1006 1785 761 1028 2301 Fragments arranged by size: 2301 1785 1028 1006 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2856 5403 5488 6559 2856 Size: 2547 85 1071 3639 Fragments arranged by size: 3639 2547 1071 85 BsmI GAATG_Cn' Cuts at: 4853 4946 4853 Size: 93 7249 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2580 2856 3547 4289 4661 5403 5488 Size: 399 790 276 691 742 372 742 85 Cuts at: 5488 6559 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 790 742 742 691 399 372 276 85 BspEI T'CCGG_A Cuts at: 7005 7005 Size: 7342 BspHI T'CATG_A Cuts at: 5231 6961 5231 Size: 1730 5612 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2490 4697 844 Size: 1646 2207 3489 Fragments arranged by size: 3489 2207 1646 BsrBI CCG'CTC Cuts at: 1394 1823 2751 5229 1394 Size: 429 928 2478 3507 Fragments arranged by size: 3507 2478 928 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2427 2856 3750 3900 3929 3999 4201 Size: 1183 1179 429 894 150 29 70 202 Cuts at: 4201 4609 5837 6011 65 Size: 408 1228 174 1396 Fragments arranged by size: 1396 1228 1183 1179 894 429 408 202 174 150 70 29 BsrFI r'CCGG_y Cuts at: 1700 1810 5986 6173 1700 Size: 110 4176 187 2869 Fragments arranged by size: 4176 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3294 3773 4329 96 Size: 3198 479 556 3109 Fragments arranged by size: 3198 3109 556 479 BssHII G'CGCG_C Cuts at: 1925 2810 2916 1925 Size: 885 106 6351 Fragments arranged by size: 6351 885 106 BssSI C'ACGA_G Cuts at: 5402 6414 5402 Size: 1012 6330 BstAPI GCAn_nnn'nTGC Cuts at: 794 2721 3223 794 Size: 1927 502 4913 Fragments arranged by size: 4913 1927 502 BstBI TT'CG_AA Cuts at: 6146 6146 Size: 7342 BstEII G'GTnAC_C Cuts at: 1184 2114 1184 Size: 930 6412 BstXI CCAn_nnnn'nTGG Cuts at: 2473 2605 2971 2473 Size: 132 366 6844 Fragments arranged by size: 6844 366 132 BstYI r'GATC_y Cuts at: 1972 2393 2499 2613 3273 4672 5039 5424 5441 Size: 421 106 114 660 1399 367 385 17 Cuts at: 5441 6893 6979 6991 7337 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1399 660 421 385 367 346 114 106 86 17 12 BstZ17I GTA'TAC Cuts at: 4155 4155 Size: 7342 Bsu36I CC'TnA_GG Cuts at: 3702 3702 Size: 7342 BtgI C'CryG_G Cuts at: 513 1065 2604 3201 513 Size: 552 1539 597 4654 Fragments arranged by size: 4654 1539 597 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2710 487 Size: 683 56 1484 5119 Fragments arranged by size: 5119 1484 683 56 BtsI GCAGTG_nn' Cuts at: 2464 3669 4858 5652 5672 2464 Size: 1205 1189 794 20 4134 Fragments arranged by size: 4134 1205 1189 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7342 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7307 DraI TTT'AAA Cuts at: 4646 4646 Size: 7342 DraIII CAC_nnn'GTG Cuts at: 4338 4338 Size: 7342 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2641 6349 809 Size: 507 1325 3708 1802 Fragments arranged by size: 3708 1802 1325 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2898 3135 4789 Size: 54 1425 50 275 18 1068 237 1654 Cuts at: 4789 5678 7307 8 Size: 889 1629 43 Fragments arranged by size: 1654 1629 1425 1068 889 275 237 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4789 1537 Size: 275 2977 4090 Fragments arranged by size: 4090 2977 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2498 2831 5272 1353 Size: 1145 333 2441 3423 Fragments arranged by size: 3423 2441 1145 333 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2750 2984 5929 6303 6449 387 Size: 1064 138 1161 234 2945 374 146 1280 Fragments arranged by size: 2945 1280 1161 1064 374 234 146 138 EcoICRI GAG'CTC Cuts at: 2854 2854 Size: 7342 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2187 2354 2993 3077 4151 5405 6002 Size: 393 555 167 639 84 1074 1254 597 Cuts at: 6002 6789 7057 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1254 1074 787 639 597 555 393 268 167 84 EcoNI CCTnn'n_nnAGG Cuts at: 3701 3701 Size: 7342 EcoO109I rG'GnC_Cy Cuts at: 2131 4135 2131 Size: 2004 5338 EcoRI G'AATT_C Cuts at: 4651 5029 4651 Size: 378 6964 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2135 3322 2135 Size: 1187 6155 FspI TGC'GCA Cuts at: 2714 2834 5848 2714 Size: 120 3014 4208 Fragments arranged by size: 4208 3014 120 HaeII r_GCGC'y Cuts at: 1678 2532 2964 4035 6489 1678 Size: 854 432 1071 2454 2531 Fragments arranged by size: 2531 2454 1071 854 432 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2049 2081 3149 3181 3473 Size: 32 135 32 477 32 1068 32 292 Cuts at: 3473 3505 3638 3670 4509 4541 5983 6015 6057 Size: 32 133 32 839 32 1442 32 42 Cuts at: 6057 6089 1373 Size: 32 2626 Fragments arranged by size: 2626 1442 1068 839 477 292 135 133 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2016 3487 4686 4932 669 Size: 988 281 78 1471 1199 246 3079 Fragments arranged by size: 3079 1471 1199 988 281 246 78 HindIII A'AGCT_T Cuts at: 748 3254 748 Size: 2506 4836 HpaI GTT'AAC Cuts at: 4932 4932 Size: 7342 KasI G'GCGC_C Cuts at: 2960 2960 Size: 7342 KpnI G_GTAC'C Cuts at: 4667 4667 Size: 7342 MfeI C'AATT_G Cuts at: 4941 4941 Size: 7342 MluI A'CGCG_T Cuts at: 7218 7218 Size: 7342 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2778 6456 6640 1305 Size: 1473 3678 184 2007 Fragments arranged by size: 3678 2007 1473 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2900 3137 10 Size: 54 1425 343 1068 237 4215 Fragments arranged by size: 4215 1425 1068 343 237 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2471 2894 3179 4586 5300 5659 Size: 648 843 462 423 285 1407 714 359 Cuts at: 5659 5818 518 Size: 159 2042 Fragments arranged by size: 2042 1407 843 714 648 462 423 359 285 159 MspA1I CmG'CkG Cuts at: 1606 4758 5435 6583 6828 1606 Size: 3152 677 1148 245 2120 Fragments arranged by size: 3152 2120 1148 677 245 NarI GG'CG_CC Cuts at: 2961 2961 Size: 7342 NcoI C'CATG_G Cuts at: 513 1065 2604 3201 513 Size: 552 1539 597 4654 Fragments arranged by size: 4654 1539 597 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7342 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7342 NotI GC'GGCC_GC Cuts at: 4789 4789 Size: 7342 NsiI A_TGCA'T Cuts at: 2320 3782 2320 Size: 1462 5880 NspI r_CATG'y Cuts at: 1132 1229 2301 2951 3780 4216 4700 6245 1132 Size: 97 1072 650 829 436 484 1545 2229 Fragments arranged by size: 2229 1545 1072 829 650 484 436 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7342 PciI A'CATG_T Cuts at: 2297 6241 2297 Size: 3944 3398 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7342 PfoI T'CCnGG_A Cuts at: 3057 3276 3503 4128 3057 Size: 219 227 625 6271 Fragments arranged by size: 6271 625 227 219 PmeI GTTT'AAAC Cuts at: 4646 4646 Size: 7342 PmlI CAC'GTG Cuts at: 4338 4338 Size: 7342 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5411 5443 6924 6956 5411 Size: 32 1481 32 5797 Fragments arranged by size: 5797 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7342 PsiI TTA'TAA Cuts at: 4912 4912 Size: 7342 PstI C_TGCA'G Cuts at: 830 3676 4694 830 Size: 2846 1018 3478 Fragments arranged by size: 3478 2846 1018 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7342 SacI G_AGCT'C Cuts at: 2856 2856 Size: 7342 SalI G'TCGA_C Cuts at: 1936 4684 1936 Size: 2748 4594 SapI GCTCTTCn'nnn_ Cuts at: 2498 2498 Size: 7342 SbfI CC_TGCA'GG Cuts at: 4694 4694 Size: 7342 ScaI AGT'ACT Cuts at: 5590 7302 5590 Size: 1712 5630 SfcI C'TryA_G Cuts at: 826 1045 3672 4390 4690 5825 6506 6697 826 Size: 219 2627 718 300 1135 681 191 1471 Fragments arranged by size: 2627 1471 1135 718 681 300 219 191 SfoI GGC'GCC Cuts at: 2962 2962 Size: 7342 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7342 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7342 SmaI CCC'GGG Cuts at: 1872 4669 1872 Size: 2797 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5446 6347 6609 820 Size: 197 926 3503 901 262 1553 Fragments arranged by size: 3503 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5133 493 Size: 4640 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7342 SphI G_CATG'C Cuts at: 1132 4700 1132 Size: 3568 3774 SspI AAT'ATT Cuts at: 5 52 5266 5 Size: 47 5214 2081 Fragments arranged by size: 5214 2081 47 StuI AGG'CCT Cuts at: 1517 2690 1517 Size: 1173 6169 StyI C'CwwG_G Cuts at: 513 1065 2604 3201 3819 4467 4781 513 Size: 552 1539 597 618 648 314 3074 Fragments arranged by size: 3074 1539 648 618 597 552 314 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2208 2267 2884 4480 5369 5386 5539 Size: 1255 628 59 617 1596 889 17 153 Cuts at: 5539 5724 6169 325 Size: 185 445 1498 Fragments arranged by size: 1596 1498 1255 889 628 617 445 185 153 59 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3294 3773 4329 Size: 274 80 33 51 1443 1317 479 556 Cuts at: 4329 5588 7300 96 Size: 1259 1712 138 Fragments arranged by size: 1712 1443 1317 1259 556 479 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3223 3255 7302 7334 3223 Size: 32 4047 32 3231 Fragments arranged by size: 4047 3231 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7342 XbaI T'CTAG_A Cuts at: 4678 4678 Size: 7342 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3425 3757 4636 3425 Size: 332 879 6131 Fragments arranged by size: 6131 879 332 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7342 XmaI C'CCGG_G Cuts at: 1870 4667 1870 Size: 2797 4545 XmnI GAAnn'nnTTC Cuts at: 3436 5471 3436 Size: 2035 5307 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5150 276 Size: 53 83 186 936 3616 2468 Fragments arranged by size: 3616 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BsiWI BsmBI EcoRV FseI FspAI NaeI NgoMIV NruI PacI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SrfI SwaI