Restriction map for
pFN21AB8522
(Linear) (Six-base) MAPSORT of: KIBB8522.seq Check: 1477 from: 1 to: 2637
KIBB8522.orf
With 182 enzymes: SgfI *
August 16, 2021 14:16 ..
AccI GT'mk_AC
Cuts at: 0 2149 2637
Size: 2149 488
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 182 2637
Size: 182 2455
AflIII A'CryG_T
Cuts at: 0 292 635 1400 2637
Size: 292 343 765 1237
Fragments arranged by size:
1237 765 343 292
AleI CACnn'nnGTG
Cuts at: 0 889 2581 2637
Size: 889 1692 56
Fragments arranged by size:
1692 889 56
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2599 2637
Size: 2599 38
ApoI r'AATT_y
Cuts at: 0 2041 2289 2637
Size: 2041 248 348
Fragments arranged by size:
2041 348 248
AvaI C'yCGr_G
Cuts at: 0 1936 2637
Size: 1936 701
BamHI G'GATC_C
Cuts at: 0 388 2637
Size: 388 2249
BanI G'GyrC_C
Cuts at: 0 955 2137 2637
Size: 955 1182 500
Fragments arranged by size:
1182 955 500
BanII G_rGCy'C
Cuts at: 0 851 2637
Size: 851 1786
BbeI G_GCGC'C
Cuts at: 0 959 2637
Size: 959 1678
BbsI GAAGACnn'nnnn_
Cuts at: 0 191 1724 2637
Size: 191 1533 913
Fragments arranged by size:
1533 913 191
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1519 1553 1823 1857 2637
Size: 1519 34 270 34 780
Fragments arranged by size:
1519 780 270 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 282 297 2637
Size: 282 15 2340
Fragments arranged by size:
2340 282 15
BfrBI ATG'CAT
Cuts at: 0 313 1775 2637
Size: 313 1462 862
Fragments arranged by size:
1462 862 313
BglI GCCn_nnn'nGGC
Cuts at: 0 2429 2637
Size: 2429 208
BglII A'GATC_T
Cuts at: 0 608 2637
Size: 608 2029
Bme1580I G_kGCm'C
Cuts at: 0 575 1542 2284 2637
Size: 575 967 742 353
Fragments arranged by size:
967 742 575 353
BmrI ACTGGGnnnn_n'
Cuts at: 0 1464 1515 1732 2048 2555 2637
Size: 1464 51 217 316 507 82
Fragments arranged by size:
1464 507 316 217 82 51
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 993 2637
Size: 993 1644
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 349 988 1072 2146 2637
Size: 349 639 84 1074 491
Fragments arranged by size:
1074 639 491 349 84
Bpu10I CC'TnA_GC
Cuts at: 0 33 2637
Size: 33 2604
BsaAI yAC'GTr
Cuts at: 0 636 2333 2579 2637
Size: 636 1697 246 58
Fragments arranged by size:
1697 636 246 58
BsaBI GATnn'nnATC
Cuts at: 0 1591 2637
Size: 1591 1046
BsaHI Gr'CG_yC
Cuts at: 0 956 1721 2637
Size: 956 765 916
Fragments arranged by size:
956 916 765
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1057 1087 2637
Size: 1057 30 1550
Fragments arranged by size:
1550 1057 30
BseYI C'CCAG_C
Cuts at: 0 905 980 1941 2637
Size: 905 75 961 696
Fragments arranged by size:
961 905 696 75
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1101 1545 2413 2500 2637
Size: 1101 444 868 87 137
Fragments arranged by size:
1101 868 444 137 87
BsiEI CG_ry'CG
Cuts at: 0 1002 2637
Size: 1002 1635
BsiHKAI G_wGCw'C
Cuts at: 0 851 2637
Size: 851 1786
Bsp1286I G_dGCh'C
Cuts at: 0 575 851 1542 2284 2637
Size: 575 276 691 742 353
Fragments arranged by size:
742 691 575 353 276
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 485 2637
Size: 485 2152
BsrBI CCG'CTC
Cuts at: 0 746 2637
Size: 746 1891
BsrDI GCAATG_nn'
Cuts at: 0 422 851 1745 1895 1924 1994 2196 2604
Size: 422 429 894 150 29 70 202 408
Cuts at: 2604 2637
Size: 33
Fragments arranged by size:
894 429 422 408 202 150 70 33
29
BsrGI T'GTAC_A
Cuts at: 0 1289 1768 2324 2637
Size: 1289 479 556 313
Fragments arranged by size:
1289 556 479 313
BssHII G'CGCG_C
Cuts at: 0 805 911 2637
Size: 805 106 1726
Fragments arranged by size:
1726 805 106
BstAPI GCAn_nnn'nTGC
Cuts at: 0 716 1218 2637
Size: 716 502 1419
Fragments arranged by size:
1419 716 502
BstEII G'GTnAC_C
Cuts at: 0 109 2637
Size: 109 2528
BstXI CCAn_nnnn'nTGG
Cuts at: 0 468 600 966 2637
Size: 468 132 366 1671
Fragments arranged by size:
1671 468 366 132
BstYI r'GATC_y
Cuts at: 0 388 494 608 1268 2637
Size: 388 106 114 660 1369
Fragments arranged by size:
1369 660 388 114 106
BstZ17I GTA'TAC
Cuts at: 0 2150 2637
Size: 2150 487
Bsu36I CC'TnA_GG
Cuts at: 0 1697 2637
Size: 1697 940
BtgI C'CryG_G
Cuts at: 0 599 1196 2637
Size: 599 597 1441
Fragments arranged by size:
1441 599 597
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 705 2637
Size: 705 1932
BtsI GCAGTG_nn'
Cuts at: 0 459 1664 2637
Size: 459 1205 973
Fragments arranged by size:
1205 973 459
DraIII CAC_nnn'GTG
Cuts at: 0 2333 2637
Size: 2333 304
DrdI GACnn_nn'nnGTC
Cuts at: 0 636 2637
Size: 636 2001
EaeI y'GGCC_r
Cuts at: 0 893 1130 2637
Size: 893 237 1507
Fragments arranged by size:
1507 893 237
EarI CTCTTCn'nnn_
Cuts at: 0 493 826 2637
Size: 493 333 1811
Fragments arranged by size:
1811 493 333
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 745 979 2637
Size: 745 234 1658
Fragments arranged by size:
1658 745 234
EcoICRI GAG'CTC
Cuts at: 0 849 2637
Size: 849 1788
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 182 349 988 1072 2146 2637
Size: 182 167 639 84 1074 491
Fragments arranged by size:
1074 639 491 182 167 84
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1696 2637
Size: 1696 941
EcoO109I rG'GnC_Cy
Cuts at: 0 126 2130 2637
Size: 126 2004 507
Fragments arranged by size:
2004 507 126
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 130 1317 2637
Size: 130 1187 1320
Fragments arranged by size:
1320 1187 130
FspI TGC'GCA
Cuts at: 0 709 829 2637
Size: 709 120 1808
Fragments arranged by size:
1808 709 120
HaeII r_GCGC'y
Cuts at: 0 527 959 2030 2637
Size: 527 432 1071 607
Fragments arranged by size:
1071 607 527 432
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 44 76 1144 1176 1468 1500 1633 1665
Size: 44 32 1068 32 292 32 133 32
Cuts at: 1665 2504 2536 2637
Size: 839 32 101
Fragments arranged by size:
1068 839 292 133 101 44 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 11 1482 2637
Size: 11 1471 1155
Fragments arranged by size:
1471 1155 11
HindIII A'AGCT_T
Cuts at: 0 1249 2637
Size: 1249 1388
KasI G'GCGC_C
Cuts at: 0 955 2637
Size: 955 1682
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 773 2637
Size: 773 1864
MscI TGG'CCA
Cuts at: 0 895 1132 2637
Size: 895 237 1505
Fragments arranged by size:
1505 895 237
MslI CAynn'nnrTG
Cuts at: 0 466 889 1174 2581 2637
Size: 466 423 285 1407 56
Fragments arranged by size:
1407 466 423 285 56
NarI GG'CG_CC
Cuts at: 0 956 2637
Size: 956 1681
NcoI C'CATG_G
Cuts at: 0 599 1196 2637
Size: 599 597 1441
Fragments arranged by size:
1441 599 597
NsiI A_TGCA'T
Cuts at: 0 315 1777 2637
Size: 315 1462 860
Fragments arranged by size:
1462 860 315
NspI r_CATG'y
Cuts at: 0 296 946 1775 2211 2637
Size: 296 650 829 436 426
Fragments arranged by size:
829 650 436 426 296
PciI A'CATG_T
Cuts at: 0 292 2637
Size: 292 2345
PfoI T'CCnGG_A
Cuts at: 0 1052 1271 1498 2123 2637
Size: 1052 219 227 625 514
Fragments arranged by size:
1052 625 514 227 219
PmlI CAC'GTG
Cuts at: 0 2333 2637
Size: 2333 304
PstI C_TGCA'G
Cuts at: 0 1671 2637
Size: 1671 966
SacI G_AGCT'C
Cuts at: 0 851 2637
Size: 851 1786
SapI GCTCTTCn'nnn_
Cuts at: 0 493 2637
Size: 493 2144
SfcI C'TryA_G
Cuts at: 0 1667 2385 2637
Size: 1667 718 252
Fragments arranged by size:
1667 718 252
SfoI GGC'GCC
Cuts at: 0 957 2637
Size: 957 1680
StuI AGG'CCT
Cuts at: 0 685 2637
Size: 685 1952
StyI C'CwwG_G
Cuts at: 0 599 1196 1814 2462 2637
Size: 599 597 618 648 175
Fragments arranged by size:
648 618 599 597 175
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 203 262 879 2475 2637
Size: 203 59 617 1596 162
Fragments arranged by size:
1596 617 203 162 59
TatI w'GTAC_w
Cuts at: 0 1289 1768 2324 2637
Size: 1289 479 556 313
Fragments arranged by size:
1289 556 479 313
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1218 1250 2637
Size: 1218 32 1387
Fragments arranged by size:
1387 1218 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1420 1752 2637
Size: 1420 332 885
Fragments arranged by size:
1420 885 332
XmnI GAAnn'nnTTC
Cuts at: 0 1431 2637
Size: 1431 1206
Enzymes that do cut:
AccI AcuI AflIII AleI AlfI ApoI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BfrBI BglI
BglII Bme1580I BmrI BplI BpmI Bpu10I BsaAI BsaBI
BsaHI BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI
BsrBI BsrDI BsrGI BssHII BstAPI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI DraIII DrdI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI
HaeII Hin4I HincII HindIII KasI MmeI MscI MslI
NarI NcoI NsiI NspI PciI PfoI PmlI PstI
SacI SapI SfcI SfoI StuI StyI TaqII TatI
TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BbvCI BclI BlpI BmgBI BmtI BpuEI BsaI
BsaWI BseRI BsiWI BsmI BsmBI BspEI BspHI BsrFI
BssSI BstBI ClaI CspCI DraI EagI EcoRI EcoRV
FseI FspAI HpaI KpnI MfeI MluI MspA1I NaeI
NdeI NgoMIV NheI NotI NruI PacI PasI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI PvuII RsrII SacII SalI SanDI SbfI ScaI
SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI SwaI Tth111I XbaI XhoI XmaI
ZraI