Restriction map for pFN21AB8522
 (Linear) (Six-base) MAPSORT of: KIBB8522.seq  Check: 1477  from: 1  to: 2637



KIBB8522.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:32  ..

AccI GT'mk_AC

Cuts at:      0    2149    2637 
   Size:       2149     488
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     182    2637 
   Size:        182    2455
 
AflIII A'CryG_T

Cuts at:      0     292     635    1400    2637 
   Size:        292     343     765    1237

  Fragments arranged by size: 

                1237     765     343     292
 
AleI CACnn'nnGTG

Cuts at:      0     889    2581    2637 
   Size:        889    1692      56

  Fragments arranged by size: 

                1692     889      56
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2599    2637 
   Size:       2599      38
 
ApoI r'AATT_y

Cuts at:      0    2041    2289    2637 
   Size:       2041     248     348

  Fragments arranged by size: 

                2041     348     248
 
AvaI C'yCGr_G

Cuts at:      0    1936    2637 
   Size:       1936     701
 
BamHI G'GATC_C

Cuts at:      0     388    2637 
   Size:        388    2249
 
BanI G'GyrC_C

Cuts at:      0     955    2137    2637 
   Size:        955    1182     500

  Fragments arranged by size: 

                1182     955     500
 
BanII G_rGCy'C

Cuts at:      0     851    2637 
   Size:        851    1786
 
BbeI G_GCGC'C

Cuts at:      0     959    2637 
   Size:        959    1678
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     191    1724    2637 
   Size:        191    1533     913

  Fragments arranged by size: 

                1533     913     191
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1519    1553    1823    1857    2637 
   Size:       1519      34     270      34     780

  Fragments arranged by size: 

                1519     780     270      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     282     297    2637 
   Size:        282      15    2340

  Fragments arranged by size: 

                2340     282      15
 
BfrBI ATG'CAT

Cuts at:      0     313    1775    2637 
   Size:        313    1462     862

  Fragments arranged by size: 

                1462     862     313
 
BglI GCCn_nnn'nGGC

Cuts at:      0    2429    2637 
   Size:       2429     208
 
BglII A'GATC_T

Cuts at:      0     608    2637 
   Size:        608    2029
 
Bme1580I G_kGCm'C

Cuts at:      0     575    1542    2284    2637 
   Size:        575     967     742     353

  Fragments arranged by size: 

                 967     742     575     353
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1464    1515    1732    2048    2555    2637 
   Size:       1464      51     217     316     507      82

  Fragments arranged by size: 

                1464     507     316     217      82      51
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     993    2637 
   Size:        993    1644
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     349     988    1072    2146    2637 
   Size:        349     639      84    1074     491

  Fragments arranged by size: 

                1074     639     491     349      84
 
Bpu10I CC'TnA_GC

Cuts at:      0      33    2637 
   Size:         33    2604
 
BsaAI yAC'GTr

Cuts at:      0     636    2333    2579    2637 
   Size:        636    1697     246      58

  Fragments arranged by size: 

                1697     636     246      58
 
BsaBI GATnn'nnATC

Cuts at:      0    1591    2637 
   Size:       1591    1046
 
BsaHI Gr'CG_yC

Cuts at:      0     956    1721    2637 
   Size:        956     765     916

  Fragments arranged by size: 

                 956     916     765
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1057    1087    2637 
   Size:       1057      30    1550

  Fragments arranged by size: 

                1550    1057      30
 
BseYI C'CCAG_C

Cuts at:      0     905     980    1941    2637 
   Size:        905      75     961     696

  Fragments arranged by size: 

                 961     905     696      75
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1101    1545    2413    2500    2637 
   Size:       1101     444     868      87     137

  Fragments arranged by size: 

                1101     868     444     137      87
 
BsiEI CG_ry'CG

Cuts at:      0    1002    2637 
   Size:       1002    1635
 
BsiHKAI G_wGCw'C

Cuts at:      0     851    2637 
   Size:        851    1786
 
Bsp1286I G_dGCh'C

Cuts at:      0     575     851    1542    2284    2637 
   Size:        575     276     691     742     353

  Fragments arranged by size: 

                 742     691     575     353     276
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     485    2637 
   Size:        485    2152
 
BsrBI CCG'CTC

Cuts at:      0     746    2637 
   Size:        746    1891
 
BsrDI GCAATG_nn'

Cuts at:      0     422     851    1745    1895    1924    1994    2196    2604 
   Size:        422     429     894     150      29      70     202     408

Cuts at:   2604    2637 
   Size:         33

  Fragments arranged by size: 

                 894     429     422     408     202     150      70      33
                  29
 
BsrGI T'GTAC_A

Cuts at:      0    1289    1768    2324    2637 
   Size:       1289     479     556     313

  Fragments arranged by size: 

                1289     556     479     313
 
BssHII G'CGCG_C

Cuts at:      0     805     911    2637 
   Size:        805     106    1726

  Fragments arranged by size: 

                1726     805     106
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     716    1218    2637 
   Size:        716     502    1419

  Fragments arranged by size: 

                1419     716     502
 
BstEII G'GTnAC_C

Cuts at:      0     109    2637 
   Size:        109    2528
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     468     600     966    2637 
   Size:        468     132     366    1671

  Fragments arranged by size: 

                1671     468     366     132
 
BstYI r'GATC_y

Cuts at:      0     388     494     608    1268    2637 
   Size:        388     106     114     660    1369

  Fragments arranged by size: 

                1369     660     388     114     106
 
BstZ17I GTA'TAC

Cuts at:      0    2150    2637 
   Size:       2150     487
 
Bsu36I CC'TnA_GG

Cuts at:      0    1697    2637 
   Size:       1697     940
 
BtgI C'CryG_G

Cuts at:      0     599    1196    2637 
   Size:        599     597    1441

  Fragments arranged by size: 

                1441     599     597
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     705    2637 
   Size:        705    1932
 
BtsI GCAGTG_nn'

Cuts at:      0     459    1664    2637 
   Size:        459    1205     973

  Fragments arranged by size: 

                1205     973     459
 
DraIII CAC_nnn'GTG

Cuts at:      0    2333    2637 
   Size:       2333     304
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     636    2637 
   Size:        636    2001
 
EaeI y'GGCC_r

Cuts at:      0     893    1130    2637 
   Size:        893     237    1507

  Fragments arranged by size: 

                1507     893     237
 
EarI CTCTTCn'nnn_

Cuts at:      0     493     826    2637 
   Size:        493     333    1811

  Fragments arranged by size: 

                1811     493     333
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     745     979    2637 
   Size:        745     234    1658

  Fragments arranged by size: 

                1658     745     234
 
EcoICRI GAG'CTC

Cuts at:      0     849    2637 
   Size:        849    1788
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     182     349     988    1072    2146    2637 
   Size:        182     167     639      84    1074     491

  Fragments arranged by size: 

                1074     639     491     182     167      84
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1696    2637 
   Size:       1696     941
 
EcoO109I rG'GnC_Cy

Cuts at:      0     126    2130    2637 
   Size:        126    2004     507

  Fragments arranged by size: 

                2004     507     126
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     130    1317    2637 
   Size:        130    1187    1320

  Fragments arranged by size: 

                1320    1187     130
 
FspI TGC'GCA

Cuts at:      0     709     829    2637 
   Size:        709     120    1808

  Fragments arranged by size: 

                1808     709     120
 
HaeII r_GCGC'y

Cuts at:      0     527     959    2030    2637 
   Size:        527     432    1071     607

  Fragments arranged by size: 

                1071     607     527     432
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      44      76    1144    1176    1468    1500    1633    1665 
   Size:         44      32    1068      32     292      32     133      32

Cuts at:   1665    2504    2536    2637 
   Size:        839      32     101

  Fragments arranged by size: 

                1068     839     292     133     101      44      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0      11    1482    2637 
   Size:         11    1471    1155

  Fragments arranged by size: 

                1471    1155      11
 
HindIII A'AGCT_T

Cuts at:      0    1249    2637 
   Size:       1249    1388
 
KasI G'GCGC_C

Cuts at:      0     955    2637 
   Size:        955    1682
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     773    2637 
   Size:        773    1864
 
MscI TGG'CCA

Cuts at:      0     895    1132    2637 
   Size:        895     237    1505

  Fragments arranged by size: 

                1505     895     237
 
MslI CAynn'nnrTG

Cuts at:      0     466     889    1174    2581    2637 
   Size:        466     423     285    1407      56

  Fragments arranged by size: 

                1407     466     423     285      56
 
NarI GG'CG_CC

Cuts at:      0     956    2637 
   Size:        956    1681
 
NcoI C'CATG_G

Cuts at:      0     599    1196    2637 
   Size:        599     597    1441

  Fragments arranged by size: 

                1441     599     597
 
NsiI A_TGCA'T

Cuts at:      0     315    1777    2637 
   Size:        315    1462     860

  Fragments arranged by size: 

                1462     860     315
 
NspI r_CATG'y

Cuts at:      0     296     946    1775    2211    2637 
   Size:        296     650     829     436     426

  Fragments arranged by size: 

                 829     650     436     426     296
 
PciI A'CATG_T

Cuts at:      0     292    2637 
   Size:        292    2345
 
PfoI T'CCnGG_A

Cuts at:      0    1052    1271    1498    2123    2637 
   Size:       1052     219     227     625     514

  Fragments arranged by size: 

                1052     625     514     227     219
 
PmlI CAC'GTG

Cuts at:      0    2333    2637 
   Size:       2333     304
 
PstI C_TGCA'G

Cuts at:      0    1671    2637 
   Size:       1671     966
 
SacI G_AGCT'C

Cuts at:      0     851    2637 
   Size:        851    1786
 
SapI GCTCTTCn'nnn_

Cuts at:      0     493    2637 
   Size:        493    2144
 
SfcI C'TryA_G

Cuts at:      0    1667    2385    2637 
   Size:       1667     718     252

  Fragments arranged by size: 

                1667     718     252
 
SfoI GGC'GCC

Cuts at:      0     957    2637 
   Size:        957    1680
 
StuI AGG'CCT

Cuts at:      0     685    2637 
   Size:        685    1952
 
StyI C'CwwG_G

Cuts at:      0     599    1196    1814    2462    2637 
   Size:        599     597     618     648     175

  Fragments arranged by size: 

                 648     618     599     597     175
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     203     262     879    2475    2637 
   Size:        203      59     617    1596     162

  Fragments arranged by size: 

                1596     617     203     162      59
 
TatI w'GTAC_w

Cuts at:      0    1289    1768    2324    2637 
   Size:       1289     479     556     313

  Fragments arranged by size: 

                1289     556     479     313
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1218    1250    2637 
   Size:       1218      32    1387

  Fragments arranged by size: 

                1387    1218      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1420    1752    2637 
   Size:       1420     332     885

  Fragments arranged by size: 

                1420     885     332
 
XmnI GAAnn'nnTTC

Cuts at:      0    1431    2637 
   Size:       1431    1206
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AleI     AlfI     ApoI     AvaI    BamHI
     BanI    BanII     BbeI     BbsI     BcgI    BciVI    BfrBI     BglI
    BglII Bme1580I     BmrI     BplI     BpmI   Bpu10I    BsaAI    BsaBI
    BsaHI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspMI
    BsrBI    BsrDI    BsrGI   BssHII   BstAPI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI   DraIII     DrdI     EaeI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI     FspI
    HaeII    Hin4I   HincII  HindIII     KasI     MmeI     MscI     MslI
     NarI     NcoI     NsiI     NspI     PciI     PfoI     PmlI     PstI
     SacI     SapI     SfcI     SfoI     StuI     StyI    TaqII     TatI
     TstI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI    BbvCI     BclI     BlpI    BmgBI     BmtI    BpuEI     BsaI
    BsaWI    BseRI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrFI
    BssSI    BstBI     ClaI    CspCI     DraI     EagI    EcoRI    EcoRV
     FseI    FspAI     HpaI     KpnI     MfeI     MluI   MspA1I     NaeI
     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PasI    PflMI
     PmeI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    PvuII    RsrII    SacII     SalI    SanDI     SbfI     ScaI
    SexAI     SfiI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI  Tth111I     XbaI     XhoI     XmaI
     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com