(Circular) (Six-base) MAPSORT of: pFN21AE0979.seq Check: 6990 from: 1 to: 6427 pFN21AE0979 6427 bp With 182 enzymes: SgfI * August 16, 2010 14:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2108 3194 2108 Size: 1086 5341 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4237 278 Size: 53 83 186 936 2701 2468 Fragments arranged by size: 2701 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2589 3770 1937 Size: 652 1181 4594 Fragments arranged by size: 4594 1181 652 Acc65I G'GTAC_C Cuts at: 3616 3748 3616 Size: 132 6295 AclI AA'CG_TT Cuts at: 1563 4554 4927 1563 Size: 2991 373 3063 Fragments arranged by size: 3063 2991 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2097 2274 2303 2673 4490 5874 6142 1632 Size: 465 177 29 370 1817 1384 268 1917 Fragments arranged by size: 1917 1817 1384 465 370 268 177 29 AfeI AGC'GCT Cuts at: 2855 2855 Size: 6427 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6230 AflIII A'CryG_T Cuts at: 1203 3192 5326 6303 1203 Size: 1989 2134 977 1327 Fragments arranged by size: 2134 1989 1327 977 AgeI A'CCGG_T Cuts at: 2064 5258 2064 Size: 3194 3233 AhdI GACnn_n'nnGTC Cuts at: 3196 5156 3196 Size: 1960 4467 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6427 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6427 AlwNI CAG_nnn'CTG Cuts at: 2336 4170 5742 2336 Size: 1834 1572 3021 Fragments arranged by size: 3021 1834 1572 ApaLI G'TGCA_C Cuts at: 4484 5640 4484 Size: 1156 5271 ApoI r'AATT_y Cuts at: 1493 2104 2443 2639 2796 2875 3535 3736 3962 Size: 611 339 196 157 79 660 201 226 Cuts at: 3962 4114 6223 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 660 611 339 226 201 196 157 152 79 AseI AT'TA_AT Cuts at: 160 1032 4981 160 Size: 872 3949 1606 Fragments arranged by size: 3949 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6427 AvaI C'yCGr_G Cuts at: 1870 1943 3752 1870 Size: 73 1809 4545 Fragments arranged by size: 4545 1809 73 AvrII C'CTAG_G Cuts at: 3119 3119 Size: 6427 BamHI G'GATC_C Cuts at: 3299 3757 4124 3299 Size: 458 367 5602 Fragments arranged by size: 5602 458 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2565 3616 3748 5203 618 Size: 325 217 627 778 1051 132 1455 1842 Fragments arranged by size: 1842 1455 1051 778 627 325 217 132 BanII G_rGCy'C Cuts at: 1391 2912 3746 1391 Size: 1521 834 4072 Fragments arranged by size: 4072 1521 834 BbeI G_GCGC'C Cuts at: 2569 2569 Size: 6427 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3485 928 Size: 971 1586 3870 Fragments arranged by size: 3870 1586 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4618 4652 1903 Size: 34 2681 34 3678 Fragments arranged by size: 3678 2681 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4319 5529 4319 Size: 1210 5217 BclI T'GATC_A Cuts at: 1548 2386 1548 Size: 838 5589 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 3122 5038 Size: 107 122 71 631 605 337 1113 1916 Cuts at: 5038 136 Size: 1525 Fragments arranged by size: 1916 1525 1113 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 3655 6422 3655 Size: 2767 3660 BlpI GC'TnA_GC Cuts at: 2658 3844 2658 Size: 1186 5241 Bme1580I G_kGCm'C Cuts at: 1790 3170 4488 5644 1790 Size: 1380 1318 1156 2573 Fragments arranged by size: 2573 1380 1318 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6427 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2720 4158 5116 Size: 416 53 83 399 24 1303 1438 958 Cuts at: 5116 442 Size: 1753 Fragments arranged by size: 1753 1438 1303 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3048 1056 Size: 1992 4435 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2435 3501 5087 1239 Size: 1196 1066 1586 2579 Fragments arranged by size: 2579 1586 1196 1066 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3397 4552 5417 5715 3397 Size: 1155 865 298 4109 Fragments arranged by size: 4109 1155 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5090 882 Size: 617 3591 2219 Fragments arranged by size: 3591 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4218 493 Size: 711 3014 2702 Fragments arranged by size: 3014 2702 711 BsaBI GATnn'nnATC Cuts at: 3660 6421 3660 Size: 2761 3666 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2012 2566 3103 4234 Size: 53 83 186 936 479 554 537 1131 Cuts at: 4234 4616 5251 275 Size: 382 635 1451 Fragments arranged by size: 1451 1131 936 635 554 537 479 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2064 4860 5258 5532 5679 6090 2064 Size: 2796 398 274 147 411 2401 Fragments arranged by size: 2796 2401 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2408 2438 1178 Size: 30 165 30 1005 30 5167 Fragments arranged by size: 5167 1005 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6427 BseYI C'CCAG_C Cuts at: 2055 2336 2543 3040 5630 6126 2055 Size: 281 207 497 2590 496 2356 Fragments arranged by size: 2590 2356 497 496 281 207 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5854 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3877 4638 5666 1540 Size: 275 186 1876 761 1028 2301 Fragments arranged by size: 2301 1876 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3148 4488 4573 5644 3148 Size: 1340 85 1071 3931 Fragments arranged by size: 3931 1340 1071 85 BsmI GAATG_Cn' Cuts at: 2505 3938 4031 2505 Size: 1433 93 4901 Fragments arranged by size: 4901 1433 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2912 3148 3170 3746 4488 4573 5644 Size: 399 1122 236 22 576 742 85 1071 Cuts at: 5644 1391 Size: 2174 Fragments arranged by size: 2174 1122 1071 742 576 399 236 85 22 BspEI T'CCGG_A Cuts at: 6090 6090 Size: 6427 BspHI T'CATG_A Cuts at: 2925 4316 6046 2925 Size: 1391 1730 3306 Fragments arranged by size: 3306 1730 1391 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2108 3100 3116 3194 3645 3782 844 Size: 1264 992 16 78 451 137 3489 Fragments arranged by size: 3489 1264 992 451 137 78 16 BsrBI CCG'CTC Cuts at: 1394 1823 4314 1394 Size: 429 2491 3507 Fragments arranged by size: 3507 2491 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4922 5096 65 Size: 1183 3674 174 1396 Fragments arranged by size: 3674 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2064 5071 5258 1700 Size: 110 254 3007 187 2869 Fragments arranged by size: 3007 2869 254 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6427 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6427 BssSI C'ACGA_G Cuts at: 4487 5499 4487 Size: 1012 5415 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6427 BstBI TT'CG_AA Cuts at: 5231 5231 Size: 6427 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6427 BstYI r'GATC_y Cuts at: 1972 2831 3299 3655 3757 4124 4509 4526 5978 Size: 859 468 356 102 367 385 17 1452 Cuts at: 5978 6064 6076 6422 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 859 468 385 367 356 346 102 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2733 2733 Size: 6427 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4936 Fragments arranged by size: 4936 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5688 Fragments arranged by size: 5688 683 56 BtsI GCAGTG_nn' Cuts at: 2922 3943 4737 4757 2922 Size: 1021 794 20 4592 Fragments arranged by size: 4592 1021 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6427 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2925 2960 529 Size: 35 2361 35 3996 Fragments arranged by size: 3996 2361 35 35 DraI TTT'AAA Cuts at: 2088 2110 3544 3712 3731 2088 Size: 22 1434 168 19 4784 Fragments arranged by size: 4784 1434 168 22 19 DraIII CAC_nnn'GTG Cuts at: 2720 2720 Size: 6427 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5434 809 Size: 507 4118 1802 Fragments arranged by size: 4118 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2226 3874 4763 Size: 54 1425 50 275 18 396 1648 889 Cuts at: 4763 6392 8 Size: 1629 43 Fragments arranged by size: 1648 1629 1425 889 396 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3874 1537 Size: 275 2062 4090 Fragments arranged by size: 4090 2062 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2250 4357 1353 Size: 897 2107 3423 Fragments arranged by size: 3423 2107 897 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5014 5388 5534 387 Size: 1064 138 3425 374 146 1280 Fragments arranged by size: 3425 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2097 2274 2303 2435 2673 3501 4490 Size: 393 465 177 29 132 238 828 989 Cuts at: 4490 5087 5874 6142 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 989 828 787 597 465 393 268 238 177 132 29 EcoNI CCTnn'n_nnAGG Cuts at: 3123 3123 Size: 6427 EcoO109I rG'GnC_Cy Cuts at: 2249 2249 Size: 6427 EcoRI G'AATT_C Cuts at: 2443 3535 3736 4114 2443 Size: 1092 201 378 4756 Fragments arranged by size: 4756 1092 378 201 EcoRV GAT'ATC Cuts at: 3239 3239 Size: 6427 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2599 2599 Size: 6427 FspI TGC'GCA Cuts at: 4933 4933 Size: 6427 HaeII r_GCGC'y Cuts at: 1678 2569 2857 5574 1678 Size: 891 288 2717 2531 Fragments arranged by size: 2717 2531 891 288 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2408 2440 2735 2767 2939 Size: 32 135 32 836 32 295 32 172 Cuts at: 2939 2971 3182 3214 3297 3329 5068 5100 5142 Size: 32 211 32 83 32 1739 32 42 Cuts at: 5142 5174 1373 Size: 32 2626 Fragments arranged by size: 2626 1739 836 295 211 172 135 83 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3429 3771 4017 669 Size: 988 281 1491 342 246 3079 Fragments arranged by size: 3079 1491 988 342 281 246 HindIII A'AGCT_T Cuts at: 748 2141 2493 2763 3492 3627 3690 748 Size: 1393 352 270 729 135 63 3485 Fragments arranged by size: 3485 1393 729 352 270 135 63 HpaI GTT'AAC Cuts at: 3429 4017 3429 Size: 588 5839 KasI G'GCGC_C Cuts at: 2565 2565 Size: 6427 KpnI G_GTAC'C Cuts at: 3620 3752 3620 Size: 132 6295 MfeI C'AATT_G Cuts at: 3186 4026 3186 Size: 840 5587 MluI A'CGCG_T Cuts at: 6303 6303 Size: 6427 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2309 5541 5725 1305 Size: 1004 3232 184 2007 Fragments arranged by size: 3232 2007 1004 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2228 10 Size: 54 1425 343 396 4209 Fragments arranged by size: 4209 1425 396 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2532 4385 4744 4903 518 Size: 648 1366 1853 359 159 2042 Fragments arranged by size: 2042 1853 1366 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2312 3843 4520 5668 5913 1606 Size: 706 1531 677 1148 245 2120 Fragments arranged by size: 2120 1531 1148 706 677 245 NarI GG'CG_CC Cuts at: 2566 2566 Size: 6427 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4936 Fragments arranged by size: 4936 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6427 NheI G'CTAG_C Cuts at: 1052 3044 1052 Size: 1992 4435 NotI GC'GGCC_GC Cuts at: 3874 3874 Size: 6427 NspI r_CATG'y Cuts at: 1132 1229 3196 3452 3785 5330 1132 Size: 97 1967 256 333 1545 2229 Fragments arranged by size: 2229 1967 1545 333 256 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6427 PciI A'CATG_T Cuts at: 3192 5326 3192 Size: 2134 4293 PflMI CCAn_nnn'nTGG Cuts at: 1108 2720 1108 Size: 1612 4815 PfoI T'CCnGG_A Cuts at: 3517 3517 Size: 6427 PmeI GTTT'AAAC Cuts at: 3731 3731 Size: 6427 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4496 4528 6009 6041 4496 Size: 32 1481 32 4882 Fragments arranged by size: 4882 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2249 2249 Size: 6427 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6427 PsiI TTA'TAA Cuts at: 3249 3997 3249 Size: 748 5679 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2602 2634 2602 Size: 32 6395 PstI C_TGCA'G Cuts at: 830 2917 3205 3779 830 Size: 2087 288 574 3478 Fragments arranged by size: 3478 2087 574 288 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6427 PvuII CAG'CTG Cuts at: 2312 2312 Size: 6427 SalI G'TCGA_C Cuts at: 1936 3769 1936 Size: 1833 4594 SbfI CC_TGCA'GG Cuts at: 3779 3779 Size: 6427 ScaI AGT'ACT Cuts at: 4675 6387 4675 Size: 1712 4715 SfcI C'TryA_G Cuts at: 826 1045 2835 2913 3180 3201 3775 4910 5591 Size: 219 1790 78 267 21 574 1135 681 Cuts at: 5591 5782 826 Size: 191 1471 Fragments arranged by size: 1790 1471 1135 681 574 267 219 191 78 21 SfoI GGC'GCC Cuts at: 2567 2567 Size: 6427 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6427 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6427 SmaI CCC'GGG Cuts at: 1872 3754 1872 Size: 1882 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3376 4531 5432 5694 820 Size: 197 926 1433 1155 901 262 1553 Fragments arranged by size: 1553 1433 1155 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4218 493 Size: 3725 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6427 SphI G_CATG'C Cuts at: 1132 3452 3785 1132 Size: 2320 333 3774 Fragments arranged by size: 3774 2320 333 SspI AAT'ATT Cuts at: 5 52 3345 4351 5 Size: 47 3293 1006 2081 Fragments arranged by size: 3293 2081 1006 47 StuI AGG'CCT Cuts at: 1517 2841 1517 Size: 1324 5103 StyI C'CwwG_G Cuts at: 513 1065 2004 2583 2605 3119 3866 513 Size: 552 939 579 22 514 747 3074 Fragments arranged by size: 3074 939 747 579 552 514 22 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4454 4471 4624 4809 5254 325 Size: 1255 2874 17 153 185 445 1498 Fragments arranged by size: 2874 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2950 4673 6385 Size: 274 80 33 51 1443 973 1723 1712 Cuts at: 6385 96 Size: 138 Fragments arranged by size: 1723 1712 1443 973 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6387 6419 6387 Size: 32 6395 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6427 XbaI T'CTAG_A Cuts at: 2957 3652 3763 2957 Size: 695 111 5621 Fragments arranged by size: 5621 695 111 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6427 XmaI C'CCGG_G Cuts at: 1870 3752 1870 Size: 1882 4545 XmnI GAAnn'nnTTC Cuts at: 4556 4556 Size: 6427 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4235 276 Size: 53 83 186 936 2701 2468 Fragments arranged by size: 2701 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoICRI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI