Restriction map for pFN21AE0979
 (Linear) (Six-base) MAPSORT of: KIEE0979.seq  Check: 1012  from: 1  to: 1722



KIEE0979.orf

 With 182 enzymes: SgfI * 

                        August 14, 2013 11:53  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     103    1189    1722 
   Size:        103    1086     533

  Fragments arranged by size: 

                1086     533     103
 
AccI GT'mk_AC

Cuts at:      0     584    1722 
   Size:        584    1138
 
Acc65I G'GTAC_C

Cuts at:      0    1611    1722 
   Size:       1611     111
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      92     269     298     668    1722 
   Size:         92     177      29     370    1054

  Fragments arranged by size: 

                1054     370     177      92      29
 
AfeI AGC'GCT

Cuts at:      0     850    1722 
   Size:        850     872
 
AflIII A'CryG_T

Cuts at:      0    1187    1722 
   Size:       1187     535
 
AgeI A'CCGG_T

Cuts at:      0      59    1722 
   Size:         59    1663
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1191    1722 
   Size:       1191     531
 
AlwNI CAG_nnn'CTG

Cuts at:      0     331    1722 
   Size:        331    1391
 
ApoI r'AATT_y

Cuts at:      0      99     438     634     791     870    1530    1722 
   Size:         99     339     196     157      79     660     192

  Fragments arranged by size: 

                 660     339     196     192     157      99      79
 
AvrII C'CTAG_G

Cuts at:      0    1114    1722 
   Size:       1114     608
 
BamHI G'GATC_C

Cuts at:      0    1294    1722 
   Size:       1294     428
 
BanI G'GyrC_C

Cuts at:      0     560    1611    1722 
   Size:        560    1051     111

  Fragments arranged by size: 

                1051     560     111
 
BanII G_rGCy'C

Cuts at:      0     907    1722 
   Size:        907     815
 
BbeI G_GCGC'C

Cuts at:      0     564    1722 
   Size:        564    1158
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1480    1722 
   Size:       1480     242
 
BclI T'GATC_A

Cuts at:      0     381    1722 
   Size:        381    1341
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1117    1722 
   Size:       1117     605
 
BglII A'GATC_T

Cuts at:      0    1650    1722 
   Size:       1650      72
 
BlpI GC'TnA_GC

Cuts at:      0     653    1722 
   Size:        653    1069
 
Bme1580I G_kGCm'C

Cuts at:      0    1165    1722 
   Size:       1165     557
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     715    1722 
   Size:        715    1007
 
BmtI G_CTAG'C

Cuts at:      0    1043    1722 
   Size:       1043     679
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     430    1496    1722 
   Size:        430    1066     226

  Fragments arranged by size: 

                1066     430     226
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1392    1722 
   Size:       1392     330
 
BsaBI GATnn'nnATC

Cuts at:      0    1655    1722 
   Size:       1655      67
 
BsaHI Gr'CG_yC

Cuts at:      0       7     561    1098    1722 
   Size:          7     554     537     624

  Fragments arranged by size: 

                 624     554     537       7
 
BsaWI w'CCGG_w

Cuts at:      0      59    1722 
   Size:         59    1663
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     403     433    1722 
   Size:        403      30    1289

  Fragments arranged by size: 

                1289     403      30
 
BseYI C'CCAG_C

Cuts at:      0      50     331     538    1035    1722 
   Size:         50     281     207     497     687

  Fragments arranged by size: 

                 687     497     281     207      50
 
BsiHKAI G_wGCw'C

Cuts at:      0    1143    1722 
   Size:       1143     579
 
BsmI GAATG_Cn'

Cuts at:      0     500    1722 
   Size:        500    1222
 
Bsp1286I G_dGCh'C

Cuts at:      0     907    1143    1165    1722 
   Size:        907     236      22     557

  Fragments arranged by size: 

                 907     557     236      22
 
BspHI T'CATG_A

Cuts at:      0     920    1722 
   Size:        920     802
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     103    1095    1111    1189    1640    1722 
   Size:        103     992      16      78     451      82

  Fragments arranged by size: 

                 992     451     103      82      78      16
 
BsrFI r'CCGG_y

Cuts at:      0      59    1722 
   Size:         59    1663
 
BstYI r'GATC_y

Cuts at:      0     826    1294    1650    1722 
   Size:        826     468     356      72

  Fragments arranged by size: 

                 826     468     356      72
 
Bsu36I CC'TnA_GG

Cuts at:      0     728    1722 
   Size:        728     994
 
BtsI GCAGTG_nn'

Cuts at:      0     917    1722 
   Size:        917     805
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     920     955    1722 
   Size:        920      35     767

  Fragments arranged by size: 

                 920     767      35
 
DraI TTT'AAA

Cuts at:      0      83     105    1539    1707    1722 
   Size:         83      22    1434     168      15

  Fragments arranged by size: 

                1434     168      83      22      15
 
DraIII CAC_nnn'GTG

Cuts at:      0     715    1722 
   Size:        715    1007
 
EaeI y'GGCC_r

Cuts at:      0     221    1722 
   Size:        221    1501
 
EarI CTCTTCn'nnn_

Cuts at:      0     245    1722 
   Size:        245    1477
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      92     269     298     430     668    1496    1722 
   Size:         92     177      29     132     238     828     226

  Fragments arranged by size: 

                 828     238     226     177     132      92      29
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1118    1722 
   Size:       1118     604
 
EcoO109I rG'GnC_Cy

Cuts at:      0     244    1722 
   Size:        244    1478
 
EcoRI G'AATT_C

Cuts at:      0     438    1530    1722 
   Size:        438    1092     192

  Fragments arranged by size: 

                1092     438     192
 
EcoRV GAT'ATC

Cuts at:      0    1234    1722 
   Size:       1234     488
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     594    1722 
   Size:        594    1128
 
HaeII r_GCGC'y

Cuts at:      0     564     852    1722 
   Size:        564     288     870

  Fragments arranged by size: 

                 870     564     288
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     403     435     730     762     934     966    1177    1209 
   Size:        403      32     295      32     172      32     211      32

Cuts at:   1209    1292    1324    1722 
   Size:         83      32     398

  Fragments arranged by size: 

                 403     398     295     211     172      83      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0    1424    1722 
   Size:       1424     298
 
HindIII A'AGCT_T

Cuts at:      0     136     488     758    1487    1622    1685    1722 
   Size:        136     352     270     729     135      63      37

  Fragments arranged by size: 

                 729     352     270     136     135      63      37
 
HpaI GTT'AAC

Cuts at:      0    1424    1722 
   Size:       1424     298
 
KasI G'GCGC_C

Cuts at:      0     560    1722 
   Size:        560    1162
 
KpnI G_GTAC'C

Cuts at:      0    1615    1722 
   Size:       1615     107
 
MfeI C'AATT_G

Cuts at:      0    1181    1722 
   Size:       1181     541
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     304    1722 
   Size:        304    1418
 
MscI TGG'CCA

Cuts at:      0     223    1722 
   Size:        223    1499
 
MslI CAynn'nnrTG

Cuts at:      0     527    1722 
   Size:        527    1195
 
MspA1I CmG'CkG

Cuts at:      0     307    1722 
   Size:        307    1415
 
NarI GG'CG_CC

Cuts at:      0     561    1722 
   Size:        561    1161
 
NheI G'CTAG_C

Cuts at:      0    1039    1722 
   Size:       1039     683
 
NspI r_CATG'y

Cuts at:      0    1191    1447    1722 
   Size:       1191     256     275

  Fragments arranged by size: 

                1191     275     256
 
PciI A'CATG_T

Cuts at:      0    1187    1722 
   Size:       1187     535
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     715    1722 
   Size:        715    1007
 
PfoI T'CCnGG_A

Cuts at:      0    1512    1722 
   Size:       1512     210
 
PpuMI rG'GwC_Cy

Cuts at:      0     244    1722 
   Size:        244    1478
 
PsiI TTA'TAA

Cuts at:      0    1244    1722 
   Size:       1244     478
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     597     629    1722 
   Size:        597      32    1093

  Fragments arranged by size: 

                1093     597      32
 
PstI C_TGCA'G

Cuts at:      0     912    1200    1722 
   Size:        912     288     522

  Fragments arranged by size: 

                 912     522     288
 
PvuII CAG'CTG

Cuts at:      0     307    1722 
   Size:        307    1415
 
SfcI C'TryA_G

Cuts at:      0     830     908    1175    1196    1722 
   Size:        830      78     267      21     526

  Fragments arranged by size: 

                 830     526     267      78      21
 
SfoI GGC'GCC

Cuts at:      0     562    1722 
   Size:        562    1160
 
SmlI C'TyrA_G

Cuts at:      0    1371    1722 
   Size:       1371     351
 
SphI G_CATG'C

Cuts at:      0    1447    1722 
   Size:       1447     275
 
SspI AAT'ATT

Cuts at:      0    1340    1722 
   Size:       1340     382
 
StuI AGG'CCT

Cuts at:      0     836    1722 
   Size:        836     886
 
StyI C'CwwG_G

Cuts at:      0     578     600    1114    1722 
   Size:        578      22     514     608

  Fragments arranged by size: 

                 608     578     514      22
 
TatI w'GTAC_w

Cuts at:      0     945    1722 
   Size:        945     777
 
XbaI T'CTAG_A

Cuts at:      0     952    1647    1722 
   Size:        952     695      75

  Fragments arranged by size: 

                 952     695      75
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI     AfeI   AflIII     AgeI     AhdI
    AlwNI     ApoI    AvrII    BamHI     BanI    BanII     BbeI     BbsI
     BclI     BglI    BglII     BlpI Bme1580I     BmrI     BmtI     BpmI
    BpuEI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI  BsiHKAI     BsmI
 Bsp1286I    BspHI    BspMI    BsrFI    BstYI   Bsu36I     BtsI    CspCI
     DraI   DraIII     EaeI     EarI  Eco57MI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MmeI     MscI     MslI   MspA1I     NarI     NheI
     NspI     PciI    PflMI     PfoI    PpuMI     PsiI     PsrI     PstI
    PvuII     SfcI     SfoI     SmlI     SphI     SspI     StuI     StyI
     TatI     XbaI

 Enzymes that do not cut: 

    AatII     AclI    AflII     AleI     AlfI     AloI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI     BaeI    BbvCI     BcgI    BciVI
    BfrBI    BmgBI     BplI   Bpu10I     BsaI    BsaAI    BseRI     BsgI
    BsiEI    BsiWI    BsmBI    BspEI    BsrBI    BsrDI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I     BtgI    BtgZI
     ClaI     DrdI     EagI     EciI  EcoICRI     FseI     FspI    FspAI
     MluI     NaeI     NcoI     NdeI   NgoMIV     NotI     NruI     NsiI
     PacI     PasI     PmeI     PmlI     PpiI    PshAI   PspOMI    PspXI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SrfI     SwaI    TaqII     TstI  Tth111I     XcmI     XhoI     XmaI
     XmnI     ZraI
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