Restriction map for
pFN21AE1986
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# NO CUT ENZYMES ( 118 enzymes) #
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AatII AccI AccIII AclI AgeI
AhdI Alw44I ApaI ApaLI AscI
AseI AsnI AspEI AvaI AviII
AvrII BalI BamHI BbeI BbrPI
BcgI BclI BglI BglII BlnI
BlpI Bpu1102I BsaWI BseAI BsiWI
BsmBI BsmI BsoBI BspDI BspEI
BspHI BsrBI BsrDI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstYI
BstZ17I CelII ClaI CpoI DraII
DrdI EaeI EagI Eam1105I EclXI
Eco52I EcoNI EcoO109I EcoO65I EcoRI
EcoRV FbaI FseI FspI HincII
HindII HpaI KasI MfeI MflI
MluI MluNI MroI MscI MunI
NaeI NarI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspI
StuI SwaI XbaI XcmI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 62 enzymes) #
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Acc65I 1087 GGTACC G^GTACC
AccII 57 CGCG CG^CG
AcyI 3 GGCGTC G[GA]^CG[CT]C
AflII 981 CTTAAG C^TTAAG
AflIII 860 ACATGT A^C[GA][CT]GT
AlwI 52 GATCC GGATC(4/5)
AocI 176 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1289 AGCGCT AGC^GCT
Asp700I 1144 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1087 GGTACC G^GTACC
AspHI 1210 GAGCAC G[AT]GC[AT]^C
AspI 1243 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 60 GGTCC G^G[AT]CC
BbvCI 80 CCTCAGC CCTCAGC(-5/-2)
BfrI 981 CTTAAG C^TTAAG
BsaAI 304 TACGTG [CT]AC^GT[GA]
BsaBI 942 GATGTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 3 GGCGTC G[GA]^CG[CT]C
BseRI 12 CTCCTC GAGGAG(10/8)
BsgI 658 GTGCAG GTGCAG(16/14)
BsiEI 32 CGGTCG CG[GA][CT]^CG
BsiHKAI 1210 GAGCAC G[AT]GC[AT]^C
BsmFI 61 GTCCC GGGAC(10/14)
Bsp1407I 863 TGTACA T^GTACA
BspLU11I 860 ACATGT A^CATGT
BspMI 664 GCAGGT ACCTGC(4/8)
BsrFI 1020 ACCGGC [GA]^CCGG[CT]
BsrGI 863 TGTACA T^GTACA
BstUI 57 CGCG CG^CG
Bsu36I 176 CCTCAGG CC^T[ACGT]AGG
CfoI 1290 GCGC GCG^C
Cfr10I 1020 ACCGGC [GA]^CCGG[CT]
DraI 805 TTTAAA TTT^AAA
DraIII 247 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 794 CTCTTC CTCTTC(1/4)
Eco47III 1289 AGCGCT AGC^GCT
Eco81I 176 CCTCAGG CC^T[ACGT]AGG
EcoT22I 724 ATGCAT ATGCA^T
HaeII 1289 AGCGCT [GA]GCGC^[CT]
HgaI 4 GCGTC GACGC(5/10)
HhaI 1290 GCGC GCG^C
Hin1I 3 GGCGTC G[GA]^CG[CT]C
HinP1I 1290 GCGC G^CGC
HindIII 1618 AAGCTT A^AGCTT
KpnI 1087 GGTACC GGTAC^C
Ksp632I 794 CTCTTC CTCTTC(1/4)
KspI 56 CCGCGG CCGC^GG
MaeII 305 ACGT A^CGT
MamI 942 GATGTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 57 CGCG CG^CG
NdeI 1108 CATATG CA^TATG
NsiI 724 ATGCAT ATGCA^T
PflFI 1243 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 246 CCACACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacII 56 CCGCGG CCGC^GG
ScaI 998 AGTACT AGT^ACT
SmlI 981 CTTAAG C^T[CT][GA]AG
SphI 137 GCATGC GCATG^C
SspBI 863 TGTACA T^GTACA
Tth111I 1243 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 246 CCACACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1144 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
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AcsI 568 GAATTT [GA]^AATT[CT]
637 AAATTC
AlwNI 86 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
577 CAGAAGCTG
ApoI 568 GAATTT [GA]^AATT[CT]
637 AAATTC
BanII 976 GAGCCC G[GA]GC[CT]^C
1536 GGGCTC
BbsI 1016 GAAGAC GAAGAC(2/6)
27 GTCTTC
BciVI 592 GGATAC GTATCC(6/5)
428 GTATCC
BpmI 1386 CTCCAG CTGGAG(16/14)
491 CTGGAG
BpuAI 1016 GAAGAC GAAGAC(2/6)
27 GTCTTC
BsaI 102 GAGACC GGTCTC(1/5)
1422 GGTCTC
BstXI 1046 CCACAGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1107 CCATATGGGTGG
FauI 55 CCCGC CCCGC(4/6)
765 GCGGG
MaeIII 893 GTGAC ^GT[ACGT]AC
1447 GTGAC
MspA1I 56 CCGCGG C[AC]G^C[GT]G
469 CCGCTG
NspI 137 GCATGC [GA]CATG^[CT]
860 ACATGT
PleI 1509 GACTC GAGTC(4/5)
1633 GAGTC
TaqI 1014 TCGA T^CGA
1077 TCGA
Tsp45I 893 GTGAC ^GT[GC]AC
1447 GTGAC
Tsp509I 569 AATT ^AATT
638 AATT
TthHB8I 1014 TCGA T^CGA
1077 TCGA
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# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AfaI AluI BanI BbvI
BcnI BfaI BmyI BsaJI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstAPI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DsaI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI MaeI MboI
MboII MnlI MseI MslI MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TfiI Tru9I TseI TspRI