(Linear) (Six-base) MAPSORT of: KIBB8130.seq Check: 6784 from: 1 to: 1341 KIBB8130.orf With 182 enzymes: SgfI * July 15, 2010 18:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 435 1341 Size: 435 906 AatII G_ACGT'C Cuts at: 0 439 1341 Size: 439 902 AccI GT'mk_AC Cuts at: 0 716 1341 Size: 716 625 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 415 478 1341 Size: 415 63 863 Fragments arranged by size: 863 415 63 AflII C'TTAA_G Cuts at: 0 415 1341 Size: 415 926 AflIII A'CryG_T Cuts at: 0 203 563 1341 Size: 203 360 778 Fragments arranged by size: 778 360 203 AlwNI CAG_nnn'CTG Cuts at: 0 551 682 1341 Size: 551 131 659 Fragments arranged by size: 659 551 131 AvaI C'yCGr_G Cuts at: 0 893 1131 1151 1341 Size: 893 238 20 190 Fragments arranged by size: 893 238 190 20 BanI G'GyrC_C Cuts at: 0 447 596 1259 1341 Size: 447 149 663 82 Fragments arranged by size: 663 447 149 82 BanII G_rGCy'C Cuts at: 0 490 736 1341 Size: 490 246 605 Fragments arranged by size: 605 490 246 BbsI GAAGACnn'nnnn_ Cuts at: 0 67 1341 Size: 67 1274 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 693 727 1341 Size: 693 34 614 Fragments arranged by size: 693 614 34 BciVI GTATCCnnnnn_n' Cuts at: 0 379 1341 Size: 379 962 BclI T'GATC_A Cuts at: 0 671 1341 Size: 671 670 BglI GCCn_nnn'nGGC Cuts at: 0 875 1341 Size: 875 466 BmgBI CAC'GTC Cuts at: 0 204 858 1116 1341 Size: 204 654 258 225 Fragments arranged by size: 654 258 225 204 BmrI ACTGGGnnnn_n' Cuts at: 0 806 1341 Size: 806 535 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 50 277 1144 1341 Size: 50 227 867 197 Fragments arranged by size: 867 227 197 50 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 232 1147 1341 Size: 232 915 194 Fragments arranged by size: 915 232 194 BsaI GGTCTCn'nnnn_ Cuts at: 0 550 943 1341 Size: 550 393 398 Fragments arranged by size: 550 398 393 BsaHI Gr'CG_yC Cuts at: 0 436 1341 Size: 436 905 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 514 520 544 550 1341 Size: 514 6 24 6 791 Fragments arranged by size: 791 514 24 6 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 280 834 1341 Size: 280 554 507 Fragments arranged by size: 554 507 280 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1231 1341 Size: 1231 110 BsiEI CG_ry'CG Cuts at: 0 138 879 1098 1341 Size: 138 741 219 243 Fragments arranged by size: 741 243 219 138 BsiHKAI G_wGCw'C Cuts at: 0 1163 1341 Size: 1163 178 BsmI GAATG_Cn' Cuts at: 0 780 1341 Size: 780 561 Bsp1286I G_dGCh'C Cuts at: 0 490 736 1163 1341 Size: 490 246 427 178 Fragments arranged by size: 490 427 246 178 BspHI T'CATG_A Cuts at: 0 1058 1341 Size: 1058 283 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 435 587 1341 Size: 435 152 754 Fragments arranged by size: 754 435 152 BsrBI CCG'CTC Cuts at: 0 821 1341 Size: 821 520 BsrDI GCAATG_nn' Cuts at: 0 1277 1341 Size: 1277 64 BsrFI r'CCGG_y Cuts at: 0 642 1196 1341 Size: 642 554 145 Fragments arranged by size: 642 554 145 BsrGI T'GTAC_A Cuts at: 0 206 1304 1341 Size: 206 1098 37 Fragments arranged by size: 1098 206 37 BssSI C'ACGA_G Cuts at: 0 226 1341 Size: 226 1115 BstYI r'GATC_y Cuts at: 0 468 497 1241 1341 Size: 468 29 744 100 Fragments arranged by size: 744 468 100 29 Bsu36I CC'TnA_GG Cuts at: 0 803 1341 Size: 803 538 BtgI C'CryG_G Cuts at: 0 428 464 1168 1341 Size: 428 36 704 173 Fragments arranged by size: 704 428 173 36 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1269 1341 Size: 1269 72 BtsI GCAGTG_nn' Cuts at: 0 439 699 1341 Size: 439 260 642 Fragments arranged by size: 642 439 260 DrdI GACnn_nn'nnGTC Cuts at: 0 564 1341 Size: 564 777 EaeI y'GGCC_r Cuts at: 0 116 135 510 602 644 876 1341 Size: 116 19 375 92 42 232 465 Fragments arranged by size: 465 375 232 116 92 42 19 EagI C'GGCC_G Cuts at: 0 135 876 1341 Size: 135 741 465 Fragments arranged by size: 741 465 135 EarI CTCTTCn'nnn_ Cuts at: 0 398 715 791 1341 Size: 398 317 76 550 Fragments arranged by size: 550 398 317 76 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 586 1341 Size: 586 755 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 50 277 415 478 1144 1341 Size: 50 227 138 63 666 197 Fragments arranged by size: 666 227 197 138 63 50 EcoNI CCTnn'n_nnAGG Cuts at: 0 664 1341 Size: 664 677 EcoO109I rG'GnC_Cy Cuts at: 0 420 657 807 921 1067 1341 Size: 420 237 150 114 146 274 Fragments arranged by size: 420 274 237 150 146 114 FspI TGC'GCA Cuts at: 0 583 1341 Size: 583 758 HaeII r_GCGC'y Cuts at: 0 1197 1341 Size: 1197 144 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 64 96 1341 Size: 64 32 1245 Fragments arranged by size: 1245 64 32 HincII GTy'rAC Cuts at: 0 543 1341 Size: 543 798 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 878 1341 Size: 878 463 MscI TGG'CCA Cuts at: 0 118 512 604 1341 Size: 118 394 92 737 Fragments arranged by size: 737 394 118 92 MslI CAynn'nnrTG Cuts at: 0 1213 1341 Size: 1213 128 MspA1I CmG'CkG Cuts at: 0 70 156 763 875 1341 Size: 70 86 607 112 466 Fragments arranged by size: 607 466 112 86 70 NaeI GCC'GGC Cuts at: 0 644 1198 1341 Size: 644 554 143 Fragments arranged by size: 644 554 143 NcoI C'CATG_G Cuts at: 0 464 1341 Size: 464 877 NgoMIV G'CCGG_C Cuts at: 0 642 1196 1341 Size: 642 554 145 Fragments arranged by size: 642 554 145 NotI GC'GGCC_GC Cuts at: 0 876 1341 Size: 876 465 NspI r_CATG'y Cuts at: 0 567 756 888 1092 1338 1341 Size: 567 189 132 204 246 3 Fragments arranged by size: 567 246 204 189 132 3 PasI CC'CwG_GG Cuts at: 0 50 1341 Size: 50 1291 PciI A'CATG_T Cuts at: 0 563 1341 Size: 563 778 PfoI T'CCnGG_A Cuts at: 0 35 1313 1341 Size: 35 1278 28 Fragments arranged by size: 1278 35 28 PpuMI rG'GwC_Cy Cuts at: 0 420 657 807 921 1341 Size: 420 237 150 114 420 Fragments arranged by size: 420 420 237 150 114 PspXI vC'TCGA_Gb Cuts at: 0 1131 1341 Size: 1131 210 PstI C_TGCA'G Cuts at: 0 446 1341 Size: 446 895 PvuI CG_AT'CG Cuts at: 0 1098 1341 Size: 1098 243 PvuII CAG'CTG Cuts at: 0 70 763 1341 Size: 70 693 578 Fragments arranged by size: 693 578 70 SapI GCTCTTCn'nnn_ Cuts at: 0 791 1341 Size: 791 550 SexAI A'CCwGG_T Cuts at: 0 956 1341 Size: 956 385 SfcI C'TryA_G Cuts at: 0 442 717 1341 Size: 442 275 624 Fragments arranged by size: 624 442 275 SgrAI Cr'CCGG_yG Cuts at: 0 1196 1341 Size: 1196 145 SmaI CCC'GGG Cuts at: 0 895 1153 1341 Size: 895 258 188 Fragments arranged by size: 895 258 188 SmlI C'TyrA_G Cuts at: 0 247 415 1131 1162 1341 Size: 247 168 716 31 179 Fragments arranged by size: 716 247 179 168 31 StuI AGG'CCT Cuts at: 0 1129 1341 Size: 1129 212 StyI C'CwwG_G Cuts at: 0 464 1341 Size: 464 877 TatI w'GTAC_w Cuts at: 0 206 1304 1341 Size: 206 1098 37 Fragments arranged by size: 1098 206 37 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 514 546 1341 Size: 514 32 795 Fragments arranged by size: 795 514 32 Tth111I GACn'n_nGTC Cuts at: 0 1051 1341 Size: 1051 290 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 471 1341 Size: 471 870 XhoI C'TCGA_G Cuts at: 0 1131 1341 Size: 1131 210 XmaI C'CCGG_G Cuts at: 0 893 1151 1341 Size: 893 258 190 Fragments arranged by size: 893 258 190 ZraI GAC'GTC Cuts at: 0 437 1341 Size: 437 904 Enzymes that do cut: AarI AatII AccI AcuI AflII AflIII AlwNI AvaI BanI BanII BbsI BcgI BciVI BclI BglI BmgBI BmrI BpmI BpuEI BsaI BsaHI BsaXI BseRI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstYI Bsu36I BtgI BtgZI BtsI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I HincII MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NotI NspI PasI PciI PfoI PpuMI PspXI PstI PvuI PvuII SapI SexAI SfcI SgrAI SmaI SmlI StuI StyI TatI TstI Tth111I XcmI XhoI XmaI ZraI Enzymes that do not cut: Acc65I AclI AfeI AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BfrBI BglII BlpI Bme1580I BmtI BplI Bpu10I BsaAI BsaBI BsaWI BseYI BsiWI BsmBI BspEI BssHII BstAPI BstBI BstEII BstXI BstZ17I ClaI CspCI DraI DraIII EcoICRI EcoRI EcoRV FalI FseI FspAI HindIII HpaI KasI KpnI MfeI MluI NarI NdeI NheI NruI NsiI PacI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PsrI RsrII SacI SacII SalI SanDI SbfI ScaI SfiI SfoI SgfI SnaBI SpeI SphI SrfI SspI SwaI TaqII XbaI XmnI