################################## # NO CUT ENZYMES ( 147 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AflII AflIII AgeI AhdI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BbrPI BbsI BbvCI BcgI BfrI BglI BglII BlnI BpuAI BsaAI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco52I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FseI FspI HgaI HincII HindII HindIII HpaI KpnI Ksp632I KspI MfeI MflI MluI MroI MseI MunI MvnI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tru9I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccI 752 GTCTAC GT^[AC][GT]AC AcyI 511 GGCGCC G[GA]^CG[CT]C Alw44I 925 GTGCAC G^TGCAC AocI 76 CCTGAGG CC^T[ACGT]AGG Aor51HI 950 AGCGCT AGC^GCT ApaLI 925 GTGCAC G^TGCAC AspHI 925 GTGCAC G[AT]GC[AT]^C BalI 1145 TGGCCA TGG^CCA BanI 511 GGCGCC G^G[CT][GA]CC BanII 957 GAGCCC G[GA]GC[CT]^C BbeI 511 GGCGCC GGCGC^C BciVI 1059 GGATAC GTATCC(6/5) BcnI 28 CCCGG CC^[GC]GG BlpI 520 GCTAAGC GC^T[ACGT]AGC Bpu1102I 520 GCTAAGC GC^T[ACGT]AGC BsaBI 239 GATATGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 511 GGCGCC G[GA]^CG[CT]C BsiHKAI 925 GTGCAC G[AT]GC[AT]^C BspMI 748 GCAGGT ACCTGC(4/8) BsrI 586 ACTGG ACTGG(1/-1) BssSI 1018 CACGAG CACGAG(-5/-1) BstAPI 1023 GCAAGCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 76 CCTGAGG CC^T[ACGT]AGG CelII 520 GCTAAGC GC^T[ACGT]AGC DraII 589 GGGACCC [GA]G^G[ACGT]CC[CT] EaeI 1145 TGGCCA [CT]^GGCC[GA] Eco47III 950 AGCGCT AGC^GCT Eco81I 76 CCTGAGG CC^T[ACGT]AGG EcoO109I 589 GGGACCC [GA]G^G[ACGT]CC[CT] HapII 29 CCGG C^CGG Hin1I 511 GGCGCC G[GA]^CG[CT]C HpaII 29 CCGG C^CGG KasI 511 GGCGCC G^GCGCC MaeII 714 ACGT A^CGT MamI 239 GATATGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 1145 TGGCCA TGG^CCA MscI 1145 TGGCCA TGG^CCA MslI 120 CACAAGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 29 CCGG C^CGG NarI 511 GGCGCC GG^CGCC NciI 28 CCCGG CC^[GC]GG PleI 376 GAGTC GAGTC(4/5) PpuMI 589 GGGACCC [GA]G^G[AT]CC[CT] SfoI 511 GGCGCC GGC^GCC SmlI 656 CTCAAG C^T[CT][GA]AG TaqI 98 TCGA T^CGA Tsp509I 1084 AATT ^AATT TthHB8I 98 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AfaI 306 GTAC GT^AC 712 GTAC AlwI 568 GGATC GGATC(4/5) 828 GGATC BclI 84 TGATCA T^GATCA 720 TGATCA BfaI 65 CTAG C^TAG 811 CTAG BmyI 925 GTGCAC G[AGT]GC[ACT]^C 957 GAGCCC BseRI 106 GAGGAG GAGGAG(10/8) 388 GAGGAG Bsp1286I 925 GTGCAC G[AGT]GC[ACT]^C 957 GAGCCC BsrDI 33 CATTGC GCAATG(2/0) 963 CATTGC EcoNI 72 CCTACCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 858 CCTGGGAAAGG FbaI 84 TGATCA T^GATCA 720 TGATCA HaeIII 635 GGCC GG^CC 1146 GGCC MaeI 65 CTAG C^TAG 811 CTAG MaeIII 613 GTCAC ^GT[ACGT]AC 1000 GTCAC MboII 901 GAAGA GAAGA(8/7) 1188 GAAGA PstI 535 CTGCAG CTGCA^G 746 CTGCAG RsaI 306 GTAC GT^AC 712 GTAC TfiI 244 GAATC G^A[AT]TC 735 GAATC Tsp45I 613 GTCAC ^GT[GC]AC 1000 GTCAC TspRI 368 CAGTG CA[GC]TG[ACGT][ACGT]^ 585 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 44 enzymes) # ######################################### AciI AluI AlwNI AvaII BbvI BpmI BsaJI BsgI BsiYI BslI BsmAI BsmFI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeII HhaI HinP1I HinfI HphI ItaI MboI MnlI MspA1I MvaI MwoI NdeII NlaIII NlaIV PvuII Sau3AI Sau96I ScrFI SfaNI SfcI TseI