(Circular) (Six-base) MAPSORT of: pFN21AE1882.seq Check: 2336 from: 1 to: 5908 pFN21AE1882 5908 bp With 182 enzymes: SgfI * August 16, 2010 14:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3718 278 Size: 53 83 186 936 2182 2468 Fragments arranged by size: 2468 2182 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2758 3251 1937 Size: 821 493 4594 Fragments arranged by size: 4594 821 493 Acc65I G'GTAC_C Cuts at: 3229 3229 Size: 5908 AclI AA'CG_TT Cuts at: 1563 4035 4408 1563 Size: 2472 373 3063 Fragments arranged by size: 3063 2472 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2370 2459 3971 5355 5623 1632 Size: 738 89 1512 1384 268 1917 Fragments arranged by size: 1917 1512 1384 738 268 89 AfeI AGC'GCT Cuts at: 2957 2957 Size: 5908 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5711 AflIII A'CryG_T Cuts at: 1203 4807 5784 1203 Size: 3604 977 1327 Fragments arranged by size: 3604 1327 977 AgeI A'CCGG_T Cuts at: 4739 4739 Size: 5908 AhdI GACnn_n'nnGTC Cuts at: 4637 4637 Size: 5908 AleI CACnn'nnGTG Cuts at: 1166 2129 1166 Size: 963 4945 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2469 3099 1184 Size: 1285 630 3993 Fragments arranged by size: 3993 1285 630 AlwNI CAG_nnn'CTG Cuts at: 2449 2489 3052 3651 5223 2449 Size: 40 563 599 1572 3134 Fragments arranged by size: 3134 1572 599 563 40 ApaLI G'TGCA_C Cuts at: 2930 3965 5121 2930 Size: 1035 1156 3717 Fragments arranged by size: 3717 1156 1035 ApoI r'AATT_y Cuts at: 1493 3217 3443 3595 5704 1493 Size: 1724 226 152 2109 1697 Fragments arranged by size: 2109 1724 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4462 160 Size: 872 3430 1606 Fragments arranged by size: 3430 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5908 AvaI C'yCGr_G Cuts at: 1870 1943 3233 1870 Size: 73 1290 4545 Fragments arranged by size: 4545 1290 73 BamHI G'GATC_C Cuts at: 3238 3605 3238 Size: 367 5541 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2516 3229 4684 618 Size: 325 217 627 729 713 1455 1842 Fragments arranged by size: 1842 1455 729 713 627 325 217 BanII G_rGCy'C Cuts at: 1391 2966 3227 1391 Size: 1575 261 4072 Fragments arranged by size: 4072 1575 261 BbeI G_GCGC'C Cuts at: 2520 2520 Size: 5908 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4937 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4099 4133 1903 Size: 34 2162 34 3678 Fragments arranged by size: 3678 2162 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3058 3800 5010 3058 Size: 742 1210 3956 Fragments arranged by size: 3956 1210 742 BclI T'GATC_A Cuts at: 1548 2089 2725 1548 Size: 541 636 4731 Fragments arranged by size: 4731 636 541 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4519 136 Size: 107 122 71 631 605 2847 1525 Fragments arranged by size: 2847 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5903 5903 Size: 5908 BlpI GC'TnA_GC Cuts at: 2526 3325 2526 Size: 799 5109 Bme1580I G_kGCm'C Cuts at: 1790 2934 3969 5125 1790 Size: 1144 1035 1156 2573 Fragments arranged by size: 2573 1156 1144 1035 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5908 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2601 3639 4597 Size: 416 53 83 399 24 1184 1038 958 Cuts at: 4597 442 Size: 1753 Fragments arranged by size: 1753 1184 1038 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5908 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2789 2789 Size: 5908 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2274 2486 2726 2980 4568 1239 Size: 1035 212 240 254 1588 2579 Fragments arranged by size: 2579 1588 1035 254 240 212 Bpu10I CC'TnA_GC Cuts at: 2643 2985 2643 Size: 342 5566 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2646 4033 4898 5196 2646 Size: 1387 865 298 3358 Fragments arranged by size: 3358 1387 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4571 882 Size: 617 3072 2219 Fragments arranged by size: 3072 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3699 493 Size: 711 2495 2702 Fragments arranged by size: 2702 2495 711 BsaBI GATnn'nnATC Cuts at: 2248 5902 2248 Size: 3654 2254 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2517 3715 4097 4732 Size: 53 83 186 936 984 1198 382 635 Cuts at: 4732 275 Size: 1451 Fragments arranged by size: 1451 1198 984 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4341 4739 5013 5160 5571 4341 Size: 398 274 147 411 4678 Fragments arranged by size: 4678 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5683 Fragments arranged by size: 5683 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2126 2408 1186 Size: 940 282 4686 Fragments arranged by size: 4686 940 282 BseYI C'CCAG_C Cuts at: 2301 2476 3072 3199 5111 5607 2301 Size: 175 596 127 1912 496 2602 Fragments arranged by size: 2602 1912 596 496 175 127 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3005 3014 3146 1163 Size: 573 1269 9 132 3925 Fragments arranged by size: 3925 1269 573 132 9 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3358 4119 5147 1540 Size: 275 186 1357 761 1028 2301 Fragments arranged by size: 2301 1357 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2934 3969 4054 5125 2934 Size: 1035 85 1071 3717 Fragments arranged by size: 3717 1071 1035 85 BsmI GAATG_Cn' Cuts at: 3419 3512 3419 Size: 93 5815 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2934 2966 3227 3969 4054 5125 1391 Size: 399 1144 32 261 742 85 1071 2174 Fragments arranged by size: 2174 1144 1071 742 399 261 85 32 BspEI T'CCGG_A Cuts at: 5571 5571 Size: 5908 BspHI T'CATG_A Cuts at: 3797 5527 3797 Size: 1730 4178 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2744 3263 844 Size: 1900 519 3489 Fragments arranged by size: 3489 1900 519 BsrBI CCG'CTC Cuts at: 1394 1823 3795 1394 Size: 429 1972 3507 Fragments arranged by size: 3507 1972 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2037 2967 4403 4577 65 Size: 1183 789 930 1436 174 1396 Fragments arranged by size: 1436 1396 1183 930 789 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4552 4739 1700 Size: 110 2742 187 2869 Fragments arranged by size: 2869 2742 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5908 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5908 BssSI C'ACGA_G Cuts at: 3023 3968 4980 3023 Size: 945 1012 3951 Fragments arranged by size: 3951 1012 945 BstAPI GCAn_nnn'nTGC Cuts at: 794 3034 794 Size: 2240 3668 BstBI TT'CG_AA Cuts at: 4712 4712 Size: 5908 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5908 BstYI r'GATC_y Cuts at: 1972 3238 3605 3990 4007 5459 5545 5557 5903 Size: 1266 367 385 17 1452 86 12 346 Cuts at: 5903 1972 Size: 1977 Fragments arranged by size: 1977 1452 1266 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2082 2082 Size: 5908 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4417 Fragments arranged by size: 4417 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5169 Fragments arranged by size: 5169 683 56 BtsI GCAGTG_nn' Cuts at: 3424 4218 4238 3424 Size: 794 20 5094 Fragments arranged by size: 5094 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5908 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5873 DraI TTT'AAA Cuts at: 3212 3212 Size: 5908 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4915 809 Size: 507 3599 1802 Fragments arranged by size: 3599 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3150 3355 4244 Size: 54 1425 50 275 18 1320 205 889 Cuts at: 4244 5873 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1320 889 275 205 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3355 1537 Size: 275 1543 4090 Fragments arranged by size: 4090 1543 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3838 1353 Size: 2485 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 4495 4869 5015 387 Size: 1064 138 435 2471 374 146 1280 Fragments arranged by size: 2471 1280 1064 435 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2274 2370 2459 2486 2726 2980 3971 Size: 393 642 96 89 27 240 254 991 Cuts at: 3971 4568 5355 5623 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 991 787 642 597 393 268 254 240 96 89 27 EcoNI CCTnn'n_nnAGG Cuts at: 2081 2867 2081 Size: 786 5122 EcoO109I rG'GnC_Cy Cuts at: 2595 2595 Size: 5908 EcoRI G'AATT_C Cuts at: 3217 3595 3217 Size: 378 5530 FspI TGC'GCA Cuts at: 4414 4414 Size: 5908 HaeII r_GCGC'y Cuts at: 1678 2320 2520 2533 2695 2814 2959 5055 1678 Size: 642 200 13 162 119 145 2096 2531 Fragments arranged by size: 2531 2096 642 200 162 145 119 13 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2642 2674 4549 4581 4623 Size: 32 135 32 1070 32 1875 32 42 Cuts at: 4623 4655 1373 Size: 32 2626 Fragments arranged by size: 2626 1875 1070 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3252 3498 669 Size: 988 281 1314 246 3079 Fragments arranged by size: 3079 1314 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5908 HpaI GTT'AAC Cuts at: 3498 3498 Size: 5908 KasI G'GCGC_C Cuts at: 2516 2516 Size: 5908 KpnI G_GTAC'C Cuts at: 3233 3233 Size: 5908 MfeI C'AATT_G Cuts at: 3507 3507 Size: 5908 MluI A'CGCG_T Cuts at: 5784 5784 Size: 5908 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5022 5206 1305 Size: 3717 184 2007 Fragments arranged by size: 3717 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3152 10 Size: 54 1425 343 1320 2766 Fragments arranged by size: 2766 1425 1320 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2129 3866 4225 4384 518 Size: 648 963 1737 359 159 2042 Fragments arranged by size: 2042 1737 963 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2428 2449 2545 2714 3019 3324 4001 5149 Size: 822 21 96 169 305 305 677 1148 Cuts at: 5149 5394 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 822 677 305 305 245 169 96 21 NarI GG'CG_CC Cuts at: 2517 2517 Size: 5908 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4417 Fragments arranged by size: 4417 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5908 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5908 NotI GC'GGCC_GC Cuts at: 3355 3355 Size: 5908 NspI r_CATG'y Cuts at: 1132 1229 3266 4811 1132 Size: 97 2037 1545 2229 Fragments arranged by size: 2229 2037 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5908 PciI A'CATG_T Cuts at: 4807 4807 Size: 5908 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5908 PmeI GTTT'AAAC Cuts at: 3212 3212 Size: 5908 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3977 4009 5490 5522 3977 Size: 32 1481 32 4363 Fragments arranged by size: 4363 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2595 2595 Size: 5908 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5908 PsiI TTA'TAA Cuts at: 3478 3478 Size: 5908 PstI C_TGCA'G Cuts at: 830 2544 2755 3260 830 Size: 1714 211 505 3478 Fragments arranged by size: 3478 1714 505 211 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5908 PvuII CAG'CTG Cuts at: 2428 2449 2545 2714 3019 2428 Size: 21 96 169 305 5317 Fragments arranged by size: 5317 305 169 96 21 SalI G'TCGA_C Cuts at: 1936 3250 1936 Size: 1314 4594 SanDI GG'GwC_CC Cuts at: 2595 2595 Size: 5908 SbfI CC_TGCA'GG Cuts at: 3260 3260 Size: 5908 ScaI AGT'ACT Cuts at: 4156 5868 4156 Size: 1712 4196 SfcI C'TryA_G Cuts at: 826 1045 2540 2751 2759 2784 2852 2901 3256 Size: 219 1495 211 8 25 68 49 355 Cuts at: 3256 4391 5072 5263 826 Size: 1135 681 191 1471 Fragments arranged by size: 1495 1471 1135 681 355 219 211 191 68 49 25 8 SfoI GGC'GCC Cuts at: 2518 2518 Size: 5908 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5908 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5908 SmaI CCC'GGG Cuts at: 1872 3235 1872 Size: 1363 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2661 4012 4913 5175 820 Size: 197 926 718 1351 901 262 1553 Fragments arranged by size: 1553 1351 926 901 718 262 197 SnaBI TAC'GTA Cuts at: 493 3699 493 Size: 3206 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5908 SphI G_CATG'C Cuts at: 1132 3266 1132 Size: 2134 3774 SspI AAT'ATT Cuts at: 5 52 3832 5 Size: 47 3780 2081 Fragments arranged by size: 3780 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5908 StyI C'CwwG_G Cuts at: 513 1065 2004 3347 513 Size: 552 939 1343 3074 Fragments arranged by size: 3074 1343 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3086 3935 3952 4105 4290 4735 325 Size: 1255 1506 849 17 153 185 445 1498 Fragments arranged by size: 1506 1498 1255 849 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4154 5866 96 Size: 274 80 33 51 1443 2177 1712 138 Fragments arranged by size: 2177 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5868 5900 5868 Size: 32 5876 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5908 XbaI T'CTAG_A Cuts at: 3244 3244 Size: 5908 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5908 XmaI C'CCGG_G Cuts at: 1870 3233 1870 Size: 1363 4545 XmnI GAAnn'nnTTC Cuts at: 4037 4037 Size: 5908 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3716 276 Size: 53 83 186 936 2182 2468 Fragments arranged by size: 2468 2182 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SrfI SwaI XcmI