(Circular) (Six-base) MAPSORT of: pFN21AE1880.seq Check: 624 from: 1 to: 5923 pFN21AE1880 5923 bp With 182 enzymes: SgfI * January 31, 2011 17:47 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3062 3062 Size: 5923 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3733 278 Size: 53 83 186 936 2197 2468 Fragments arranged by size: 2468 2197 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2199 3266 1937 Size: 262 1067 4594 Fragments arranged by size: 4594 1067 262 Acc65I G'GTAC_C Cuts at: 3244 3244 Size: 5923 AclI AA'CG_TT Cuts at: 1563 3222 4050 4423 1563 Size: 1659 828 373 3063 Fragments arranged by size: 3063 1659 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2912 3986 5370 5638 1632 Size: 1280 1074 1384 268 1917 Fragments arranged by size: 1917 1384 1280 1074 268 AflII C'TTAA_G Cuts at: 820 1017 3127 820 Size: 197 2110 3616 Fragments arranged by size: 3616 2110 197 AflIII A'CryG_T Cuts at: 1203 4822 5799 1203 Size: 3619 977 1327 Fragments arranged by size: 3619 1327 977 AgeI A'CCGG_T Cuts at: 4754 4754 Size: 5923 AhdI GACnn_n'nnGTC Cuts at: 4652 4652 Size: 5923 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5923 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5923 AlwNI CAG_nnn'CTG Cuts at: 3666 5238 3666 Size: 1572 4351 ApaLI G'TGCA_C Cuts at: 3980 5136 3980 Size: 1156 4767 ApoI r'AATT_y Cuts at: 1493 2876 3232 3458 3610 5719 1493 Size: 1383 356 226 152 2109 1697 Fragments arranged by size: 2109 1697 1383 356 226 152 AseI AT'TA_AT Cuts at: 160 1032 4477 160 Size: 872 3445 1606 Fragments arranged by size: 3445 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5923 AvaI C'yCGr_G Cuts at: 1870 1943 2114 2291 3248 1870 Size: 73 171 177 957 4545 Fragments arranged by size: 4545 957 177 171 73 BamHI G'GATC_C Cuts at: 2487 3253 3620 2487 Size: 766 367 4790 Fragments arranged by size: 4790 766 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2567 3244 4699 618 Size: 325 217 627 780 677 1455 1842 Fragments arranged by size: 1842 1455 780 677 627 325 217 BanII G_rGCy'C Cuts at: 1391 2751 2984 3242 1391 Size: 1360 233 258 4072 Fragments arranged by size: 4072 1360 258 233 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2975 3054 3212 928 Size: 971 1076 79 158 3639 Fragments arranged by size: 3639 1076 971 158 79 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2129 2163 2306 2340 4114 4148 1903 Size: 34 192 34 143 34 1774 34 3678 Fragments arranged by size: 3678 1774 192 143 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3815 5025 3815 Size: 1210 4713 BclI T'GATC_A Cuts at: 1548 2577 1548 Size: 1029 4894 BfrBI ATG'CAT Cuts at: 2931 2931 Size: 5923 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4534 136 Size: 107 122 71 631 605 2862 1525 Fragments arranged by size: 2862 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5918 5918 Size: 5923 BlpI GC'TnA_GC Cuts at: 3340 3340 Size: 5923 Bme1580I G_kGCm'C Cuts at: 1790 2014 3984 5140 1790 Size: 224 1970 1156 2573 Fragments arranged by size: 2573 1970 1156 224 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5923 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2471 3129 3205 Size: 416 53 83 399 24 1054 658 76 Cuts at: 3205 3654 4612 442 Size: 449 958 1753 Fragments arranged by size: 1753 1054 958 658 449 416 399 83 76 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5923 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3116 4583 1239 Size: 1877 1467 2579 Fragments arranged by size: 2579 1877 1467 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4048 4913 5211 4048 Size: 865 298 4760 Fragments arranged by size: 4760 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4586 882 Size: 617 3087 2219 Fragments arranged by size: 3087 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3714 493 Size: 711 2510 2702 Fragments arranged by size: 2702 2510 711 BsaBI GATnn'nnATC Cuts at: 2795 5917 2795 Size: 3122 2801 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3730 4112 4747 275 Size: 53 83 186 936 2197 382 635 1451 Fragments arranged by size: 2197 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4356 4754 5028 5175 5586 4356 Size: 398 274 147 411 4693 Fragments arranged by size: 4693 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2181 2211 2358 2388 1178 Size: 30 165 30 778 30 147 30 4713 Fragments arranged by size: 4713 778 165 147 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3113 1186 Size: 1927 3996 BseYI C'CCAG_C Cuts at: 2853 5126 5622 2853 Size: 2273 496 3154 Fragments arranged by size: 3154 2273 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2501 3014 1163 Size: 573 765 513 4072 Fragments arranged by size: 4072 765 573 513 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3373 4134 5162 1540 Size: 275 186 1372 761 1028 2301 Fragments arranged by size: 2301 1372 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2142 2319 3984 4069 5140 2142 Size: 177 1665 85 1071 2925 Fragments arranged by size: 2925 1665 1071 177 85 BsmI GAATG_Cn' Cuts at: 3434 3527 3434 Size: 93 5830 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2014 2142 2319 2751 2984 3242 3984 Size: 399 224 128 177 432 233 258 742 Cuts at: 3984 4069 5140 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 432 399 258 233 224 177 128 85 BspEI T'CCGG_A Cuts at: 5586 5586 Size: 5923 BspHI T'CATG_A Cuts at: 3812 5542 3812 Size: 1730 4193 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3062 3278 844 Size: 2218 216 3489 Fragments arranged by size: 3489 2218 216 BsrBI CCG'CTC Cuts at: 1394 1823 3810 1394 Size: 429 1987 3507 Fragments arranged by size: 3507 1987 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2025 2077 2652 4418 4592 65 Size: 1183 777 52 575 1766 174 1396 Fragments arranged by size: 1766 1396 1183 777 575 174 52 BsrFI r'CCGG_y Cuts at: 1700 1810 4567 4754 1700 Size: 110 2757 187 2869 Fragments arranged by size: 2869 2757 187 110 BsrGI T'GTAC_A Cuts at: 96 2376 96 Size: 2280 3643 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5923 BssSI C'ACGA_G Cuts at: 3983 4995 3983 Size: 1012 4911 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5923 BstBI TT'CG_AA Cuts at: 4727 4727 Size: 5923 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5923 BstYI r'GATC_y Cuts at: 1972 2487 3123 3253 3620 4005 4022 5474 5560 Size: 515 636 130 367 385 17 1452 86 Cuts at: 5560 5572 5918 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 636 515 385 367 346 130 86 17 12 BstZ17I GTA'TAC Cuts at: 2200 2200 Size: 5923 BtgI C'CryG_G Cuts at: 513 1065 2601 513 Size: 552 1536 3835 Fragments arranged by size: 3835 1536 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3078 487 Size: 683 56 1852 3332 Fragments arranged by size: 3332 1852 683 56 BtsI GCAGTG_nn' Cuts at: 2064 2228 2405 3079 3439 4233 4253 2064 Size: 164 177 674 360 794 20 3734 Fragments arranged by size: 3734 794 674 360 177 164 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5923 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5888 DraI TTT'AAA Cuts at: 2875 3227 2875 Size: 352 5571 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4930 809 Size: 507 3614 1802 Fragments arranged by size: 3614 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3370 4259 5888 Size: 54 1425 50 275 18 1540 889 1629 Cuts at: 5888 8 Size: 43 Fragments arranged by size: 1629 1540 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3370 1537 Size: 275 1558 4090 Fragments arranged by size: 4090 1558 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2621 2972 3853 1353 Size: 1268 351 881 3423 Fragments arranged by size: 3423 1268 881 351 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4510 4884 5030 387 Size: 1064 138 2921 374 146 1280 Fragments arranged by size: 2921 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2912 3116 3986 4583 5370 5638 1239 Size: 393 1280 204 870 597 787 268 1524 Fragments arranged by size: 1524 1280 870 787 597 393 268 204 EcoNI CCTnn'n_nnAGG Cuts at: 3098 3098 Size: 5923 EcoRI G'AATT_C Cuts at: 3232 3610 3232 Size: 378 5545 EcoRV GAT'ATC Cuts at: 2086 2086 Size: 5923 FspI TGC'GCA Cuts at: 2180 2357 4429 2180 Size: 177 2072 3674 Fragments arranged by size: 3674 2072 177 HaeII r_GCGC'y Cuts at: 1678 5070 1678 Size: 3392 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4564 4596 4638 4670 1373 Size: 32 135 32 2992 32 42 32 2626 Fragments arranged by size: 2992 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3267 3513 669 Size: 988 281 1329 246 3079 Fragments arranged by size: 3079 1329 988 281 246 HindIII A'AGCT_T Cuts at: 748 3130 748 Size: 2382 3541 HpaI GTT'AAC Cuts at: 3513 3513 Size: 5923 KpnI G_GTAC'C Cuts at: 3248 3248 Size: 5923 MfeI C'AATT_G Cuts at: 3522 3522 Size: 5923 MluI A'CGCG_T Cuts at: 5799 5799 Size: 5923 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2221 2398 5037 5221 1305 Size: 916 177 2639 184 2007 Fragments arranged by size: 2639 2007 916 184 177 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4101 Fragments arranged by size: 4101 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2130 2307 2505 3881 4240 4399 518 Size: 648 964 177 198 1376 359 159 2042 Fragments arranged by size: 2042 1376 964 648 359 198 177 159 MspA1I CmG'CkG Cuts at: 1606 2485 3339 4016 5164 5409 1606 Size: 879 854 677 1148 245 2120 Fragments arranged by size: 2120 1148 879 854 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5371 NdeI CA'TA_TG Cuts at: 387 387 Size: 5923 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5923 NotI GC'GGCC_GC Cuts at: 3370 3370 Size: 5923 NsiI A_TGCA'T Cuts at: 2933 2933 Size: 5923 NspI r_CATG'y Cuts at: 1132 1229 3034 3281 4826 1132 Size: 97 1805 247 1545 2229 Fragments arranged by size: 2229 1805 1545 247 97 PasI CC'CwG_GG Cuts at: 1348 2014 1348 Size: 666 5257 PciI A'CATG_T Cuts at: 4822 4822 Size: 5923 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5923 PfoI T'CCnGG_A Cuts at: 3189 3189 Size: 5923 PmeI GTTT'AAAC Cuts at: 3227 3227 Size: 5923 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3992 4024 5505 5537 3992 Size: 32 1481 32 4378 Fragments arranged by size: 4378 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5923 PsiI TTA'TAA Cuts at: 3493 3493 Size: 5923 PstI C_TGCA'G Cuts at: 830 3275 830 Size: 2445 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5923 SalI G'TCGA_C Cuts at: 1936 3265 1936 Size: 1329 4594 SapI GCTCTTCn'nnn_ Cuts at: 2972 2972 Size: 5923 SbfI CC_TGCA'GG Cuts at: 3275 3275 Size: 5923 ScaI AGT'ACT Cuts at: 4171 5883 4171 Size: 1712 4211 SexAI A'CCwGG_T Cuts at: 3107 3107 Size: 5923 SfcI C'TryA_G Cuts at: 826 1045 2985 3271 4406 5087 5278 826 Size: 219 1940 286 1135 681 191 1471 Fragments arranged by size: 1940 1471 1135 681 286 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5923 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5923 SmaI CCC'GGG Cuts at: 1872 3250 1872 Size: 1378 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3127 4027 4928 5190 820 Size: 197 926 1184 900 901 262 1553 Fragments arranged by size: 1553 1184 926 901 900 262 197 SnaBI TAC'GTA Cuts at: 493 3714 493 Size: 3221 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5923 SphI G_CATG'C Cuts at: 1132 3281 1132 Size: 2149 3774 SspI AAT'ATT Cuts at: 5 52 3847 5 Size: 47 3795 2081 Fragments arranged by size: 3795 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5923 StyI C'CwwG_G Cuts at: 513 1065 3362 513 Size: 552 2297 3074 Fragments arranged by size: 3074 2297 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2483 3950 3967 4120 4305 4750 325 Size: 1255 903 1467 17 153 185 445 1498 Fragments arranged by size: 1498 1467 1255 903 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2376 4169 5881 Size: 274 80 33 51 1443 399 1793 1712 Cuts at: 5881 96 Size: 138 Fragments arranged by size: 1793 1712 1443 399 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2358 2390 2479 2511 5883 5915 2358 Size: 32 89 32 3372 32 2366 Fragments arranged by size: 3372 2366 89 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5923 XbaI T'CTAG_A Cuts at: 3259 3259 Size: 5923 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2741 2011 Size: 730 5193 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5923 XmaI C'CCGG_G Cuts at: 1870 3248 1870 Size: 1378 4545 XmnI GAAnn'nnTTC Cuts at: 4052 4052 Size: 5923 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3731 276 Size: 53 83 186 936 2197 2468 Fragments arranged by size: 2468 2197 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI