Restriction map for
pFN21AE1947
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# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AflIII AgeI AhdI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AvrII BamHI BbrPI BbvCI BcgI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BsgI BsiWI BsmBI BsmI
BspDI BspLU11I BsrBI BsrFI BssHII
BssSI BstBI BstXI CelII Cfr10I
ClaI CpoI DraI DraIII Eam1105I
EcoRI EcoRV EcoT22I FseI HindIII
HpaI KpnI KspI MamI MfeI
MluI MseI MunI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SbfI ScaI SfiI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspI SwaI Tru9I
Tth111I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 81 enzymes) #
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AccI 750 GTATAC GT^[AC][GT]AC
AccII 39 CGCG CG^CG
AccIII 371 TCCGGA T^CCGGA
AcyI 106 GGCGCC G[GA]^CG[CT]C
Alw44I 1264 GTGCAC G^TGCAC
AlwI 816 GGATC GGATC(4/5)
AocI 198 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1252 AGCGCT AGC^GCT
ApaI 1223 GGGCCC GGGCC^C
ApaLI 1264 GTGCAC G^TGCAC
Asp700I 1014 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1264 GTGCAC G[AT]GC[AT]^C
AvaII 366 GGACC G^G[AT]CC
AviII 89 TGCGCA TGC^GCA
BalI 1118 TGGCCA TGG^CCA
BbeI 106 GGCGCC GGCGC^C
BbsI 1321 GAAGAC GAAGAC(2/6)
BciVI 276 GGATAC GTATCC(6/5)
BfaI 970 CTAG C^TAG
BpmI 580 CTGGAG CTGGAG(16/14)
BpuAI 1321 GAAGAC GAAGAC(2/6)
BsaAI 226 TACGTG [CT]AC^GT[GA]
BsaHI 106 GGCGCC G[GA]^CG[CT]C
BsaI 136 GAGACC GGTCTC(1/5)
BsaWI 371 TCCGGA [AT]^CCGG[AT]
BseAI 371 TCCGGA T^CCGGA
BsiEI 162 CGGCCG CG[GA][CT]^CG
BsiHKAI 1264 GTGCAC G[AT]GC[AT]^C
Bsp1407I 731 TGTACA T^GTACA
BspEI 371 TCCGGA T^CCGGA
BspHI 1364 TCATGA T^CATGA
BspMI 1097 ACCTGC ACCTGC(4/8)
BsrDI 477 CATTGC GCAATG(2/0)
BsrGI 731 TGTACA T^GTACA
Bst1107I 750 GTATAC GTA^TAC
BstEII 1053 GGTTACC G^GT[ACGT]ACC
BstPI 1053 GGTTACC G^GT[ACGT]ACC
BstUI 39 CGCG CG^CG
BstYI 816 GGATCT [GA]^GATC[CT]
BstZ17I 750 GTATAC GTA^TAC
Bsu36I 198 CCTGAGG CC^T[ACGT]AGG
DrdI 1347 GACAGATGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 162 CGGCCG C^GGCCG
EclXI 162 CGGCCG C^GGCCG
Eco47III 1252 AGCGCT AGC^GCT
Eco52I 162 CGGCCG C^GGCCG
Eco81I 198 CCTGAGG CC^T[ACGT]AGG
EcoNI 369 CCTCCGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1053 GGTTACC G^GT[ACGT]ACC
FspI 89 TGCGCA TGC^GCA
HgaI 258 GACGC GACGC(5/10)
Hin1I 106 GGCGCC G[GA]^CG[CT]C
HincII 1356 GTCAAC GT[CT]^[GA]AC
HindII 1356 GTCAAC GT[CT]^[GA]AC
KasI 106 GGCGCC G^GCGCC
MaeI 970 CTAG C^TAG
MflI 816 GGATCT [GA]^GATC[CT]
MluNI 1118 TGGCCA TGG^CCA
MroI 371 TCCGGA T^CCGGA
MscI 1118 TGGCCA TGG^CCA
MvnI 39 CGCG CG^CG
NarI 106 GGCGCC GG^CGCC
NcoI 447 CCATGG C^CATGG
PflMI 1004 CCACCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 365 GGGACCT [GA]G^G[AT]CC[CT]
RcaI 1364 TCATGA T^CATGA
SapI 145 GAAGAGC GCTCTTC(1/4)
SexAI 431 ACCTGGT A^CC[AT]GGT
SfoI 106 GGCGCC GGC^GCC
SmaI 131 CCCGGG CCC^GGG
SspBI 731 TGTACA T^GTACA
StuI 565 AGGCCT AGG^CCT
TaqI 719 TCGA T^CGA
TfiI 493 GAATC G^A[AT]TC
Tsp509I 1236 AATT ^AATT
TthHB8I 719 TCGA T^CGA
Van91I 1004 CCACCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 816 GGATCT [GA]^GATC[CT]
XmaCI 131 CCCGGG C^CCGGG
XmaI 131 CCCGGG C^CCGGG
XmnI 1014 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AfaI 732 GTAC GT^AC
840 GTAC
AlwNI 741 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
814 CAGGATCTG
AvaI 131 CCCGGG C^[CT]CG[GA]G
151 CCCGAG
BclI 704 TGATCA T^GATCA
785 TGATCA
BglI 98 GCCTGGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
164 GCCGGAGGGGC
BseRI 936 CTCCTC GAGGAG(10/8)
154 GAGGAG
BsmAI 136 GAGAC GTCTC(1/5)
743 GTCTC
BsmFI 365 GGGAC GGGAC(10/14)
1125 GTCCC
BsoBI 131 CCCGGG C^[CT]CG[GA]G
151 CCCGAG
BstAPI 298 GCAGTGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1092 GCAATACCTGC
DdeI 199 CTGAG C^T[ACGT]AG
554 CTGAG
DraII 365 GGGACCT [GA]G^G[ACGT]CC[CT]
1296 AGGCCCT
EcoO109I 365 GGGACCT [GA]G^G[ACGT]CC[CT]
1296 AGGCCCT
FbaI 704 TGATCA T^GATCA
785 TGATCA
MslI 267 CATCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1173 CACTGTTATG
PstI 637 CTGCAG CTGCA^G
911 CTGCAG
PvuII 346 CAGCTG CAG^CTG
979 CAGCTG
RsaI 732 GTAC GT^AC
840 GTAC
SfcI 637 CTGCAG C^T[GA][CT]AG
911 CTGCAG
Tsp45I 460 GTCAC ^GT[GC]AC
1306 GTCAC
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# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AluI BanI BanII BbvI
BcnI BmyI BsaJI BsiYI BslI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DpnII DsaI
EaeI EarI EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MnlI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI Sau3AI Sau96I ScrFI SfaNI
StyI TseI TspRI