(Circular) (Six-base) MAPSORT of: pFN21AE1947.seq Check: 6118 from: 1 to: 6208 pFN21AE1947 6208 bp With 182 enzymes: SgfI * May 1, 2018 17:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4018 278 Size: 53 83 186 936 2482 2468 Fragments arranged by size: 2482 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2756 3551 1937 Size: 819 795 4594 Fragments arranged by size: 4594 819 795 Acc65I G'GTAC_C Cuts at: 3529 3529 Size: 6208 AclI AA'CG_TT Cuts at: 1563 4335 4708 1563 Size: 2772 373 3063 Fragments arranged by size: 3063 2772 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2700 3524 4271 5655 5923 1632 Size: 1068 824 747 1384 268 1917 Fragments arranged by size: 1917 1384 1068 824 747 268 AfeI AGC'GCT Cuts at: 3259 3259 Size: 6208 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6011 AflIII A'CryG_T Cuts at: 1203 5107 6084 1203 Size: 3904 977 1327 Fragments arranged by size: 3904 1327 977 AgeI A'CCGG_T Cuts at: 5039 5039 Size: 6208 AhdI GACnn_n'nnGTC Cuts at: 4937 4937 Size: 6208 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6208 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2102 1184 Size: 918 5290 AlwNI CAG_nnn'CTG Cuts at: 2751 2824 3951 5523 2751 Size: 73 1127 1572 3436 Fragments arranged by size: 3436 1572 1127 73 ApaI G_GGCC'C Cuts at: 3232 3232 Size: 6208 ApaLI G'TGCA_C Cuts at: 3269 4265 5421 3269 Size: 996 1156 4056 Fragments arranged by size: 4056 1156 996 ApoI r'AATT_y Cuts at: 1493 3517 3743 3895 6004 1493 Size: 2024 226 152 2109 1697 Fragments arranged by size: 2109 2024 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4762 160 Size: 872 3730 1606 Fragments arranged by size: 3730 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6208 AvaI C'yCGr_G Cuts at: 1870 1943 2136 2156 3533 1870 Size: 73 193 20 1377 4545 Fragments arranged by size: 4545 1377 193 73 20 BamHI G'GATC_C Cuts at: 3538 3905 3538 Size: 367 5841 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2066 2111 2429 2876 3005 Size: 325 217 627 279 45 318 447 129 Cuts at: 3005 3529 4984 618 Size: 524 1455 1842 Fragments arranged by size: 1842 1455 627 524 447 325 318 279 217 129 45 BanII G_rGCy'C Cuts at: 1391 2157 2166 3232 3527 1391 Size: 766 9 1066 295 4072 Fragments arranged by size: 4072 1066 766 295 9 BbeI G_GCGC'C Cuts at: 2115 2115 Size: 6208 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3333 928 Size: 971 1434 3803 Fragments arranged by size: 3803 1434 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4399 4433 1903 Size: 34 2462 34 3678 Fragments arranged by size: 3678 2462 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2275 4100 5310 2275 Size: 1825 1210 3173 Fragments arranged by size: 3173 1825 1210 BclI T'GATC_A Cuts at: 1548 2709 2790 1548 Size: 1161 81 4966 Fragments arranged by size: 4966 1161 81 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2109 2175 4819 Size: 107 122 71 631 605 437 66 2644 Cuts at: 4819 136 Size: 1525 Fragments arranged by size: 2644 1525 631 605 437 122 107 71 66 BglII A'GATC_T Cuts at: 6203 6203 Size: 6208 BlpI GC'TnA_GC Cuts at: 3625 3625 Size: 6208 Bme1580I G_kGCm'C Cuts at: 1790 2397 2432 3179 3232 3273 4269 5425 1790 Size: 607 35 747 53 41 996 1156 2573 Fragments arranged by size: 2573 1156 996 747 607 53 41 35 BmgBI CAC'GTC Cuts at: 1318 3129 1318 Size: 1811 4397 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2539 3023 3939 Size: 416 53 83 399 24 1122 484 916 Cuts at: 3939 4897 442 Size: 958 1753 Fragments arranged by size: 1753 1122 958 916 484 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6208 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2606 4868 1239 Size: 1367 2262 2579 Fragments arranged by size: 2579 2262 1367 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4333 5198 5496 4333 Size: 865 298 5045 Fragments arranged by size: 5045 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2135 4871 882 Size: 617 636 2736 2219 Fragments arranged by size: 2736 2219 636 617 BsaAI yAC'GTr Cuts at: 493 1204 2233 3999 493 Size: 711 1029 1766 2702 Fragments arranged by size: 2702 1766 1029 711 BsaBI GATnn'nnATC Cuts at: 6202 6202 Size: 6208 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2112 4015 4397 5032 Size: 53 83 186 936 579 1903 382 635 Cuts at: 5032 275 Size: 1451 Fragments arranged by size: 1903 1451 936 635 579 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2376 4641 5039 5313 5460 5871 2376 Size: 2265 398 274 147 411 2713 Fragments arranged by size: 2713 2265 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2621 2651 1178 Size: 30 165 30 1218 30 4735 Fragments arranged by size: 4735 1218 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2174 2932 1186 Size: 988 758 4462 Fragments arranged by size: 4462 988 758 BseYI C'CCAG_C Cuts at: 2070 2425 5411 5907 2070 Size: 355 2986 496 2371 Fragments arranged by size: 2986 2371 496 355 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5635 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2170 3658 4419 5447 1540 Size: 275 186 169 1488 761 1028 2301 Fragments arranged by size: 2301 1488 1028 761 275 186 169 BsiHKAI G_wGCw'C Cuts at: 3273 4269 4354 5425 3273 Size: 996 85 1071 4056 Fragments arranged by size: 4056 1071 996 85 BsmI GAATG_Cn' Cuts at: 3719 3812 3719 Size: 93 6115 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2157 2166 2397 2432 3179 3232 3273 Size: 399 367 9 231 35 747 53 41 Cuts at: 3273 3527 4269 4354 5425 1391 Size: 254 742 85 1071 2174 Fragments arranged by size: 2174 1071 747 742 399 367 254 231 85 53 41 35 9 BspEI T'CCGG_A Cuts at: 2376 5871 2376 Size: 3495 2713 BspHI T'CATG_A Cuts at: 3369 4097 5827 3369 Size: 728 1730 3750 Fragments arranged by size: 3750 1730 728 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3111 3563 844 Size: 2267 452 3489 Fragments arranged by size: 3489 2267 452 BsrBI CCG'CTC Cuts at: 1394 1823 4095 1394 Size: 429 2272 3507 Fragments arranged by size: 3507 2272 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2481 4703 4877 65 Size: 1183 1233 2222 174 1396 Fragments arranged by size: 2222 1396 1233 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4852 5039 1700 Size: 110 3042 187 2869 Fragments arranged by size: 3042 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2736 96 Size: 2640 3568 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6208 BssSI C'ACGA_G Cuts at: 4268 5280 4268 Size: 1012 5196 BstAPI GCAn_nnn'nTGC Cuts at: 794 2309 3103 794 Size: 1515 794 3899 Fragments arranged by size: 3899 1515 794 BstBI TT'CG_AA Cuts at: 5012 5012 Size: 6208 BstEII G'GTnAC_C Cuts at: 1184 3058 1184 Size: 1874 4334 BstYI r'GATC_y Cuts at: 1972 2821 3538 3905 4290 4307 5759 5845 5857 Size: 849 717 367 385 17 1452 86 12 Cuts at: 5857 6203 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 849 717 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2757 2757 Size: 6208 Bsu36I CC'TnA_GG Cuts at: 2204 2204 Size: 6208 BtgI C'CryG_G Cuts at: 513 1065 2004 2062 2244 2452 2946 3171 513 Size: 552 939 58 182 208 494 225 3550 Fragments arranged by size: 3550 939 552 494 225 208 182 58 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2318 487 Size: 683 56 1092 4377 Fragments arranged by size: 4377 1092 683 56 BtsI GCAGTG_nn' Cuts at: 2310 3724 4518 4538 2310 Size: 1414 794 20 3980 Fragments arranged by size: 3980 1414 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6208 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6173 DraI TTT'AAA Cuts at: 3512 3512 Size: 6208 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3358 5215 809 Size: 507 2042 1857 1802 Fragments arranged by size: 2042 1857 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2167 3107 3123 Size: 54 1425 50 275 18 337 940 16 Cuts at: 3123 3655 4544 6173 8 Size: 532 889 1629 43 Fragments arranged by size: 1629 1425 940 889 532 337 275 54 50 43 18 16 EagI C'GGCC_G Cuts at: 1537 1812 2167 3655 1537 Size: 275 355 1488 4090 Fragments arranged by size: 4090 1488 355 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2145 2639 3413 4138 1353 Size: 792 494 774 725 3423 Fragments arranged by size: 3423 792 774 725 494 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4795 5169 5315 387 Size: 1064 138 3206 374 146 1280 Fragments arranged by size: 3206 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2606 2700 3524 4271 4868 5655 5923 Size: 393 974 94 824 747 597 787 268 Cuts at: 5923 1239 Size: 1524 Fragments arranged by size: 1524 974 824 787 747 597 393 268 94 EcoNI CCTnn'n_nnAGG Cuts at: 2378 3503 2378 Size: 1125 5083 EcoO109I rG'GnC_Cy Cuts at: 2371 3302 2371 Size: 931 5277 EcoRI G'AATT_C Cuts at: 3517 3895 3517 Size: 378 5830 FspI TGC'GCA Cuts at: 2096 4714 2096 Size: 2618 3590 HaeII r_GCGC'y Cuts at: 1678 2115 3261 3448 5355 1678 Size: 437 1146 187 1907 2531 Fragments arranged by size: 2531 1907 1146 437 187 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3180 3212 3479 3511 4849 Size: 32 135 32 1608 32 267 32 1338 Cuts at: 4849 4881 4923 4955 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1608 1338 267 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3363 3552 3798 669 Size: 988 281 1425 189 246 3079 Fragments arranged by size: 3079 1425 988 281 246 189 HindIII A'AGCT_T Cuts at: 748 748 Size: 6208 HpaI GTT'AAC Cuts at: 3798 3798 Size: 6208 KasI G'GCGC_C Cuts at: 2111 2111 Size: 6208 KpnI G_GTAC'C Cuts at: 3533 3533 Size: 6208 MfeI C'AATT_G Cuts at: 3807 3807 Size: 6208 MluI A'CGCG_T Cuts at: 6084 6084 Size: 6208 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2550 2677 5322 5506 1305 Size: 1245 127 2645 184 2007 Fragments arranged by size: 2645 2007 1245 184 127 MscI TGG'CCA Cuts at: 10 64 1489 1832 3125 10 Size: 54 1425 343 1293 3093 Fragments arranged by size: 3093 1425 1293 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2276 3182 4166 4525 4684 518 Size: 648 1110 906 984 359 159 2042 Fragments arranged by size: 2042 1110 984 906 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2100 2353 2986 3293 3456 3624 4301 5449 Size: 494 253 633 307 163 168 677 1148 Cuts at: 5449 5694 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 633 494 307 253 245 168 163 NarI GG'CG_CC Cuts at: 2112 2112 Size: 6208 NcoI C'CATG_G Cuts at: 513 1065 2004 2452 513 Size: 552 939 448 4269 Fragments arranged by size: 4269 939 552 448 NdeI CA'TA_TG Cuts at: 387 387 Size: 6208 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6208 NotI GC'GGCC_GC Cuts at: 3655 3655 Size: 6208 NspI r_CATG'y Cuts at: 1132 1229 3566 5111 1132 Size: 97 2337 1545 2229 Fragments arranged by size: 2337 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2119 2404 1348 Size: 771 285 5152 Fragments arranged by size: 5152 771 285 PciI A'CATG_T Cuts at: 5107 5107 Size: 6208 PflMI CCAn_nnn'nTGG Cuts at: 1108 3015 1108 Size: 1907 4301 PmeI GTTT'AAAC Cuts at: 3512 3512 Size: 6208 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4277 4309 5790 5822 4277 Size: 32 1481 32 4663 Fragments arranged by size: 4663 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2371 2371 Size: 6208 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6208 PsiI TTA'TAA Cuts at: 3778 3778 Size: 6208 PspOMI G'GGCC_C Cuts at: 3228 3228 Size: 6208 PstI C_TGCA'G Cuts at: 830 2646 2920 3560 830 Size: 1816 274 640 3478 Fragments arranged by size: 3478 1816 640 274 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6208 PvuII CAG'CTG Cuts at: 2353 2986 2353 Size: 633 5575 SalI G'TCGA_C Cuts at: 1936 3550 1936 Size: 1614 4594 SapI GCTCTTCn'nnn_ Cuts at: 2145 2145 Size: 6208 SbfI CC_TGCA'GG Cuts at: 3560 3560 Size: 6208 ScaI AGT'ACT Cuts at: 4456 6168 4456 Size: 1712 4496 SexAI A'CCwGG_T Cuts at: 2436 2436 Size: 6208 SfcI C'TryA_G Cuts at: 826 1045 2642 2916 3556 4691 5372 5563 826 Size: 219 1597 274 640 1135 681 191 1471 Fragments arranged by size: 1597 1471 1135 681 640 274 219 191 SfoI GGC'GCC Cuts at: 2113 2113 Size: 6208 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6208 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6208 SmaI CCC'GGG Cuts at: 1872 2138 3535 1872 Size: 266 1397 4545 Fragments arranged by size: 4545 1397 266 SmlI C'TyrA_G Cuts at: 820 1017 1943 4312 5213 5475 820 Size: 197 926 2369 901 262 1553 Fragments arranged by size: 2369 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3999 493 Size: 3506 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6208 SphI G_CATG'C Cuts at: 1132 3566 1132 Size: 2434 3774 SspI AAT'ATT Cuts at: 5 52 4132 5 Size: 47 4080 2081 Fragments arranged by size: 4080 2081 47 StuI AGG'CCT Cuts at: 1517 2572 1517 Size: 1055 5153 StyI C'CwwG_G Cuts at: 513 1065 2004 2452 3014 3315 3647 513 Size: 552 939 448 562 301 332 3074 Fragments arranged by size: 3074 939 562 552 448 332 301 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2084 2159 2297 2417 2776 3041 4235 Size: 1255 504 75 138 120 359 265 1194 Cuts at: 4235 4252 4405 4590 5035 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1194 504 445 359 265 185 153 138 120 75 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2736 2844 4454 Size: 274 80 33 51 1443 759 108 1610 Cuts at: 4454 6166 96 Size: 1712 138 Fragments arranged by size: 1712 1610 1443 759 274 138 108 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3073 3105 6168 6200 3073 Size: 32 3063 32 3081 Fragments arranged by size: 3081 3063 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6208 XbaI T'CTAG_A Cuts at: 3544 3544 Size: 6208 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6208 XmaI C'CCGG_G Cuts at: 1870 2136 3533 1870 Size: 266 1397 4545 Fragments arranged by size: 4545 1397 266 XmnI GAAnn'nnTTC Cuts at: 3023 4337 3023 Size: 1314 4894 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4016 276 Size: 53 83 186 936 2482 2468 Fragments arranged by size: 2482 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI DraIII EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SanDI SfiI SrfI SwaI XcmI