(Linear) (Six-base) MAPSORT of: KIEE2051.seq Check: 7766 from: 1 to: 1851 KIEE2051.orf With 182 enzymes: SgfI * July 15, 2010 18:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 116 1851 Size: 116 1735 AccI GT'mk_AC Cuts at: 0 1469 1538 1851 Size: 1469 69 313 Fragments arranged by size: 1469 313 69 Acc65I G'GTAC_C Cuts at: 0 242 1851 Size: 242 1609 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 268 283 1851 Size: 268 15 1568 Fragments arranged by size: 1568 268 15 AflIII A'CryG_T Cuts at: 0 587 1197 1541 1851 Size: 587 610 344 310 Fragments arranged by size: 610 587 344 310 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 769 1851 Size: 769 1082 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 550 582 1851 Size: 550 32 1269 Fragments arranged by size: 1269 550 32 AlwNI CAG_nnn'CTG Cuts at: 0 711 915 1851 Size: 711 204 936 Fragments arranged by size: 936 711 204 ApaLI G'TGCA_C Cuts at: 0 1168 1851 Size: 1168 683 ApoI r'AATT_y Cuts at: 0 371 1851 Size: 371 1480 AvaI C'yCGr_G Cuts at: 0 359 757 993 1354 1370 1753 1851 Size: 359 398 236 361 16 383 98 Fragments arranged by size: 398 383 361 359 236 98 16 BanI G'GyrC_C Cuts at: 0 242 1851 Size: 242 1609 BanII G_rGCy'C Cuts at: 0 85 1427 1851 Size: 85 1342 424 Fragments arranged by size: 1342 424 85 BbsI GAAGACnn'nnnn_ Cuts at: 0 271 590 692 1397 1851 Size: 271 319 102 705 454 Fragments arranged by size: 705 454 319 271 102 BbvCI CC'TCA_GC Cuts at: 0 70 1851 Size: 70 1781 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1734 1768 1851 Size: 1734 34 83 Fragments arranged by size: 1734 83 34 BglI GCCn_nnn'nGGC Cuts at: 0 80 1366 1851 Size: 80 1286 485 Fragments arranged by size: 1286 485 80 BlpI GC'TnA_GC Cuts at: 0 811 1778 1851 Size: 811 967 73 Fragments arranged by size: 967 811 73 Bme1580I G_kGCm'C Cuts at: 0 1172 1298 1851 Size: 1172 126 553 Fragments arranged by size: 1172 553 126 BmgBI CAC'GTC Cuts at: 0 165 1083 1851 Size: 165 918 768 Fragments arranged by size: 918 768 165 BmrI ACTGGGnnnn_n' Cuts at: 0 193 1851 Size: 193 1658 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 820 892 1851 Size: 820 72 959 Fragments arranged by size: 959 820 72 Bpu10I CC'TnA_GC Cuts at: 0 70 1851 Size: 70 1781 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 367 480 1851 Size: 367 113 1371 Fragments arranged by size: 1371 367 113 BsaAI yAC'GTr Cuts at: 0 1200 1299 1542 1851 Size: 1200 99 243 309 Fragments arranged by size: 1200 309 243 99 BsaHI Gr'CG_yC Cuts at: 0 268 1851 Size: 268 1583 BsaWI w'CCGG_w Cuts at: 0 1826 1851 Size: 1826 25 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 550 580 1591 1621 1851 Size: 550 30 1011 30 230 Fragments arranged by size: 1011 550 230 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1516 1851 Size: 1516 335 BseYI C'CCAG_C Cuts at: 0 1646 1851 Size: 1646 205 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 729 813 885 1321 1330 1851 Size: 729 84 72 436 9 521 Fragments arranged by size: 729 521 436 84 72 9 BsiEI CG_ry'CG Cuts at: 0 1460 1851 Size: 1460 391 BsiHKAI G_wGCw'C Cuts at: 0 1172 1851 Size: 1172 679 Bsp1286I G_dGCh'C Cuts at: 0 85 1172 1298 1427 1851 Size: 85 1087 126 129 424 Fragments arranged by size: 1087 424 129 126 85 BspHI T'CATG_A Cuts at: 0 668 1851 Size: 668 1183 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 116 1851 Size: 116 1735 BsrBI CCG'CTC Cuts at: 0 663 804 922 1851 Size: 663 141 118 929 Fragments arranged by size: 929 663 141 118 BsrDI GCAATG_nn' Cuts at: 0 968 1139 1151 1211 1851 Size: 968 171 12 60 640 Fragments arranged by size: 968 640 171 60 12 BsrFI r'CCGG_y Cuts at: 0 46 313 1851 Size: 46 267 1538 Fragments arranged by size: 1538 267 46 BssSI C'ACGA_G Cuts at: 0 76 1851 Size: 76 1775 BstAPI GCAn_nnn'nTGC Cuts at: 0 1308 1851 Size: 1308 543 BstEII G'GTnAC_C Cuts at: 0 15 771 1851 Size: 15 756 1080 Fragments arranged by size: 1080 756 15 BstXI CCAn_nnnn'nTGG Cuts at: 0 555 783 1851 Size: 555 228 1068 Fragments arranged by size: 1068 555 228 BstYI r'GATC_y Cuts at: 0 191 1851 Size: 191 1660 Bsu36I CC'TnA_GG Cuts at: 0 334 1569 1851 Size: 334 1235 282 Fragments arranged by size: 1235 334 282 BtgI C'CryG_G Cuts at: 0 20 273 776 1622 1655 1730 1851 Size: 20 253 503 846 33 75 121 Fragments arranged by size: 846 503 253 121 75 33 20 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 332 568 1851 Size: 332 236 1283 Fragments arranged by size: 1283 332 236 ClaI AT'CG_AT Cuts at: 0 429 467 878 1851 Size: 429 38 411 973 Fragments arranged by size: 973 429 411 38 DrdI GACnn_nn'nnGTC Cuts at: 0 1081 1851 Size: 1081 770 EaeI y'GGCC_r Cuts at: 0 23 917 950 1049 1457 1668 1844 1851 Size: 23 894 33 99 408 211 176 7 Fragments arranged by size: 894 408 211 176 99 33 23 7 EagI C'GGCC_G Cuts at: 0 1457 1851 Size: 1457 394 EarI CTCTTCn'nnn_ Cuts at: 0 1691 1851 Size: 1691 160 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1632 1851 Size: 1632 219 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 268 283 820 892 1851 Size: 268 15 537 72 959 Fragments arranged by size: 959 537 268 72 15 EcoO109I rG'GnC_Cy Cuts at: 0 1326 1506 1851 Size: 1326 180 345 Fragments arranged by size: 1326 345 180 EcoRV GAT'ATC Cuts at: 0 501 1851 Size: 501 1350 HaeII r_GCGC'y Cuts at: 0 744 1851 Size: 744 1107 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1591 1623 1851 Size: 1591 32 228 Fragments arranged by size: 1591 228 32 HincII GTy'rAC Cuts at: 0 1662 1851 Size: 1662 189 KpnI G_GTAC'C Cuts at: 0 246 1851 Size: 246 1605 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1589 1851 Size: 1589 262 MscI TGG'CCA Cuts at: 0 25 952 1051 1846 1851 Size: 25 927 99 795 5 Fragments arranged by size: 927 795 99 25 5 MslI CAynn'nnrTG Cuts at: 0 586 781 1144 1851 Size: 586 195 363 707 Fragments arranged by size: 707 586 363 195 MspA1I CmG'CkG Cuts at: 0 198 305 1158 1605 1657 1674 1800 1851 Size: 198 107 853 447 52 17 126 51 Fragments arranged by size: 853 447 198 126 107 52 51 17 NaeI GCC'GGC Cuts at: 0 48 1851 Size: 48 1803 NcoI C'CATG_G Cuts at: 0 1730 1851 Size: 1730 121 NgoMIV G'CCGG_C Cuts at: 0 46 1851 Size: 46 1805 NspI r_CATG'y Cuts at: 0 591 1554 1689 1851 Size: 591 963 135 162 Fragments arranged by size: 963 591 162 135 PciI A'CATG_T Cuts at: 0 587 1851 Size: 587 1264 PflMI CCAn_nnn'nTGG Cuts at: 0 915 1058 1497 1851 Size: 915 143 439 354 Fragments arranged by size: 915 439 354 143 PmlI CAC'GTG Cuts at: 0 1200 1299 1851 Size: 1200 99 552 Fragments arranged by size: 1200 552 99 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 550 582 1851 Size: 550 32 1269 Fragments arranged by size: 1269 550 32 PpuMI rG'GwC_Cy Cuts at: 0 1326 1506 1851 Size: 1326 180 345 Fragments arranged by size: 1326 345 180 PspXI vC'TCGA_Gb Cuts at: 0 1753 1851 Size: 1753 98 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1522 1554 1851 Size: 1522 32 297 Fragments arranged by size: 1522 297 32 PstI C_TGCA'G Cuts at: 0 97 1520 1851 Size: 97 1423 331 Fragments arranged by size: 1423 331 97 PvuII CAG'CTG Cuts at: 0 198 1158 1605 1674 1800 1851 Size: 198 960 447 69 126 51 Fragments arranged by size: 960 447 198 126 69 51 SacII CC_GC'GG Cuts at: 0 1658 1851 Size: 1658 193 SapI GCTCTTCn'nnn_ Cuts at: 0 1691 1851 Size: 1691 160 ScaI AGT'ACT Cuts at: 0 223 1851 Size: 223 1628 SexAI A'CCwGG_T Cuts at: 0 1490 1851 Size: 1490 361 SfcI C'TryA_G Cuts at: 0 93 1516 1851 Size: 93 1423 335 Fragments arranged by size: 1423 335 93 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1366 1851 Size: 1366 485 SgrAI Cr'CCGG_yG Cuts at: 0 313 1851 Size: 313 1538 SmaI CCC'GGG Cuts at: 0 995 1372 1851 Size: 995 377 479 Fragments arranged by size: 995 479 377 SmlI C'TyrA_G Cuts at: 0 382 459 1753 1851 Size: 382 77 1294 98 Fragments arranged by size: 1294 382 98 77 SphI G_CATG'C Cuts at: 0 1554 1851 Size: 1554 297 StyI C'CwwG_G Cuts at: 0 1226 1730 1851 Size: 1226 504 121 Fragments arranged by size: 1226 504 121 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1050 1114 1575 1851 Size: 1050 64 461 276 Fragments arranged by size: 1050 461 276 64 TatI w'GTAC_w Cuts at: 0 221 251 1851 Size: 221 30 1600 Fragments arranged by size: 1600 221 30 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 896 928 1851 Size: 896 32 923 Fragments arranged by size: 923 896 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 712 1851 Size: 712 1139 XhoI C'TCGA_G Cuts at: 0 1753 1851 Size: 1753 98 XmaI C'CCGG_G Cuts at: 0 993 1370 1851 Size: 993 377 481 Fragments arranged by size: 993 481 377 XmnI GAAnn'nnTTC Cuts at: 0 565 610 844 1851 Size: 565 45 234 1007 Fragments arranged by size: 1007 565 234 45 Enzymes that do cut: AarI AccI Acc65I AcuI AflIII AlfI AloI AlwNI ApaLI ApoI AvaI BanI BanII BbsI BbvCI BcgI BglI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI ClaI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRV HaeII Hin4I HincII KpnI MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NspI PciI PflMI PmlI PpiI PpuMI PspXI PsrI PstI PvuII SacII SapI ScaI SexAI SfcI SfiI SgrAI SmaI SmlI SphI StyI TaqII TatI TstI XcmI XhoI XmaI XmnI Enzymes that do not cut: AatII AclI AfeI AflII AgeI AhdI AleI ApaI AscI AseI AsiSI AvrII BaeI BamHI BbeI BciVI BclI BfrBI BglII BmtI BplI BsaI BsaBI BsiWI BsmI BsmBI BspEI BsrGI BssHII BstBI BstZ17I BtsI CspCI DraI DraIII EcoICRI EcoNI EcoRI FalI FseI FspI FspAI HindIII HpaI KasI MfeI MluI NarI NdeI NheI NotI NruI NsiI PacI PasI PfoI PmeI PshAI PsiI PspOMI PvuI RsrII SacI SalI SanDI SbfI SfoI SgfI SnaBI SpeI SrfI SspI StuI SwaI Tth111I XbaI ZraI