################################## # NO CUT ENZYMES ( 107 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AgeI AhdI Alw44I AocI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BbeI BbrPI BbsI BbvCI BciVI BclI BfrI BlnI BpuAI BsaAI BsaBI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BsrGI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI DraIII Eam1105I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KasI KpnI MamI MfeI MluI MroI MseI MslI MunI NarI NcoI NdeI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SbfI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AccI 1030 GTCTAC GT^[AC][GT]AC AcsI 1588 GAATTT [GA]^AATT[CT] AflIII 458 ACATGT A^C[GA][CT]GT Aor51HI 302 AGCGCT AGC^GCT ApoI 1588 GAATTT [GA]^AATT[CT] Asp700I 258 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 643 CCCGGG C^[CT]CG[GA]G BamHI 1669 GGATCC G^GATCC BanI 1455 GGTGCC G^G[CT][GA]CC BcgI 504 CGACAGCTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsaI 1627 GAGACC GGTCTC(1/5) BsaWI 854 ACCGGA [AT]^CCGG[AT] BsoBI 643 CCCGGG C^[CT]CG[GA]G BspLU11I 458 ACATGT A^CATGT BspMI 920 ACCTGC ACCTGC(4/8) BsrBI 205 GAGCGG CCGCTC(-3/-3) BsrDI 1043 GCAATG GCAATG(2/0) BssHII 514 GCGCGC G^CGCGC BssSI 618 CACGAG CACGAG(-5/-1) DrdI 1021 GACATCAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 108 CCGCGG C^C[GA][CT]GG EagI 451 CGGCCG C^GGCCG EclXI 451 CGGCCG C^GGCCG Eco47III 302 AGCGCT AGC^GCT Eco52I 451 CGGCCG C^GGCCG KspI 108 CCGCGG CCGC^GG MaeII 278 ACGT A^CGT NaeI 893 GCCGGC GCC^GGC NgoMIV 893 GCCGGC G^CCGGC PflMI 1311 CCAGAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SacI 1296 GAGCTC GAGCT^C SacII 108 CCGCGG CCGC^GG SapI 858 GAAGAGC GCTCTTC(1/4) ScaI 905 AGTACT AGT^ACT SfaNI 125 GCATC GCATC(5/9) SfiI 183 GGCCAGCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 643 CCCGGG CCC^GGG SmlI 1227 CTTGAG C^T[CT][GA]AG TfiI 1163 GAATC G^A[AT]TC Tsp509I 1589 AATT ^AATT Van91I 1311 CCAGAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 32 CCAACGGGCGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 643 CCCGGG C^CCGGG XmaI 643 CCCGGG C^CCGGG XmnI 258 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AspHI 870 GAGCAC G[AT]GC[AT]^C 1296 GAGCTC BalI 182 TGGCCA TGG^CCA 950 TGGCCA BglI 184 GCCAGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 937 GCCTCCTTGGC BglII 482 AGATCT A^GATCT 1346 AGATCT BlpI 69 GCTGAGC GC^T[ACGT]AGC 390 GCTGAGC Bpu1102I 69 GCTGAGC GC^T[ACGT]AGC 390 GCTGAGC BsiEI 408 CGACCG CG[GA][CT]^CG 451 CGGCCG BsiHKAI 870 GAGCAC G[AT]GC[AT]^C 1296 GAGCTC BsrFI 893 GCCGGC [GA]^CCGG[CT] 1253 ACCGGC BstXI 14 CCAAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1439 CCAGTTCTCTGG CelII 69 GCTGAGC GC^T[ACGT]AGC 390 GCTGAGC Cfr10I 893 GCCGGC [GA]^CCGG[CT] 1253 ACCGGC EarI 1331 CTCTTC CTCTTC(1/4) 858 GAAGAG EcoNI 252 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1065 CCTGGATCAGG Ksp632I 1331 CTCTTC CTCTTC(1/4) 858 GAAGAG MluNI 182 TGGCCA TGG^CCA 950 TGGCCA MscI 182 TGGCCA TGG^CCA 950 TGGCCA NheI 339 GCTAGC G^CTAGC 552 GCTAGC NspI 458 ACATGT [GA]CATG^[CT] 977 ACATGC PleI 1690 GACTC GAGTC(4/5) 775 GAGTC SexAI 1034 ACCTGGT A^CC[AT]GGT 1574 ACCTGGT ######################################### # 3 OR MORE CUT ENZYMES ( 85 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI ApaI AvaII BanII BbvI BcnI BfaI BmyI BpmI BsaHI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfcI StuI StyI TaqI TseI Tsp45I TspRI TthHB8I XhoII