(Linear) (Six-base) MAPSORT of: KIEE1906.seq Check: 8212 from: 1 to: 1326 KIEE1906.orf With 182 enzymes: SgfI * July 15, 2010 18:50 .. AatII G_ACGT'C Cuts at: 0 485 1326 Size: 485 841 AccI GT'mk_AC Cuts at: 0 64 1326 Size: 64 1262 Acc65I G'GTAC_C Cuts at: 0 422 1326 Size: 422 904 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 163 447 1326 Size: 163 284 879 Fragments arranged by size: 879 284 163 AfeI AGC'GCT Cuts at: 0 21 631 1326 Size: 21 610 695 Fragments arranged by size: 695 610 21 AleI CACnn'nnGTG Cuts at: 0 8 1326 Size: 8 1318 AlwNI CAG_nnn'CTG Cuts at: 0 132 1326 Size: 132 1194 AvaI C'yCGr_G Cuts at: 0 178 1326 Size: 178 1148 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 761 794 1326 Size: 761 33 532 Fragments arranged by size: 761 532 33 BanI G'GyrC_C Cuts at: 0 422 847 1326 Size: 422 425 479 Fragments arranged by size: 479 425 422 BanII G_rGCy'C Cuts at: 0 179 1326 Size: 179 1147 BbsI GAAGACnn'nnnn_ Cuts at: 0 170 919 1326 Size: 170 749 407 Fragments arranged by size: 749 407 170 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 60 90 94 124 1326 Size: 60 30 4 30 1202 Fragments arranged by size: 1202 60 30 30 4 BciVI GTATCCnnnnn_n' Cuts at: 0 1079 1326 Size: 1079 247 BclI T'GATC_A Cuts at: 0 870 1326 Size: 870 456 BlpI GC'TnA_GC Cuts at: 0 16 55 1326 Size: 16 39 1271 Fragments arranged by size: 1271 39 16 BmrI ACTGGGnnnn_n' Cuts at: 0 349 1326 Size: 349 977 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 217 381 1145 1326 Size: 217 164 764 181 Fragments arranged by size: 764 217 181 164 Bpu10I CC'TnA_GC Cuts at: 0 133 173 1174 1326 Size: 133 40 1001 152 Fragments arranged by size: 1001 152 133 40 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 583 976 1326 Size: 583 393 350 Fragments arranged by size: 583 393 350 BsaBI GATnn'nnATC Cuts at: 0 205 1326 Size: 205 1121 BsaHI Gr'CG_yC Cuts at: 0 482 1326 Size: 482 844 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 291 1326 Size: 291 1035 BseYI C'CCAG_C Cuts at: 0 626 1326 Size: 626 700 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 753 1042 1326 Size: 753 289 284 Fragments arranged by size: 753 289 284 BsiEI CG_ry'CG Cuts at: 0 50 260 1326 Size: 50 210 1066 Fragments arranged by size: 1066 210 50 BsiHKAI G_wGCw'C Cuts at: 0 397 416 1326 Size: 397 19 910 Fragments arranged by size: 910 397 19 BsmI GAATG_Cn' Cuts at: 0 507 1326 Size: 507 819 BsmBI CGTCTCn'nnnn_ Cuts at: 0 489 1326 Size: 489 837 Bsp1286I G_dGCh'C Cuts at: 0 179 397 416 1326 Size: 179 218 19 910 Fragments arranged by size: 910 218 179 19 BspHI T'CATG_A Cuts at: 0 705 1326 Size: 705 621 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 76 826 1326 Size: 76 750 500 Fragments arranged by size: 750 500 76 BsrDI GCAATG_nn' Cuts at: 0 497 1326 Size: 497 829 BsrFI r'CCGG_y Cuts at: 0 974 1326 Size: 974 352 BstAPI GCAn_nnn'nTGC Cuts at: 0 392 832 1326 Size: 392 440 494 Fragments arranged by size: 494 440 392 BstYI r'GATC_y Cuts at: 0 128 1068 1326 Size: 128 940 258 Fragments arranged by size: 940 258 128 BtgI C'CryG_G Cuts at: 0 120 1326 Size: 120 1206 DraI TTT'AAA Cuts at: 0 744 1326 Size: 744 582 EaeI y'GGCC_r Cuts at: 0 257 1326 Size: 257 1069 EagI C'GGCC_G Cuts at: 0 257 1326 Size: 257 1069 EarI CTCTTCn'nnn_ Cuts at: 0 281 1326 Size: 281 1045 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 993 1326 Size: 993 333 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 163 217 381 447 1145 1326 Size: 163 54 164 66 698 181 Fragments arranged by size: 698 181 164 163 66 54 EcoO109I rG'GnC_Cy Cuts at: 0 1170 1326 Size: 1170 156 FspI TGC'GCA Cuts at: 0 100 1326 Size: 100 1226 HaeII r_GCGC'y Cuts at: 0 23 633 1326 Size: 23 610 693 Fragments arranged by size: 693 610 23 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 21 53 467 499 601 633 835 867 Size: 21 32 414 32 102 32 202 32 Cuts at: 867 906 938 1326 Size: 39 32 388 Fragments arranged by size: 414 388 202 102 39 32 32 32 32 32 21 HindIII A'AGCT_T Cuts at: 0 750 1326 Size: 750 576 KpnI G_GTAC'C Cuts at: 0 426 1326 Size: 426 900 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 646 1326 Size: 646 680 MslI CAynn'nnrTG Cuts at: 0 8 1326 Size: 8 1318 MspA1I CmG'CkG Cuts at: 0 122 1326 Size: 122 1204 NotI GC'GGCC_GC Cuts at: 0 257 1326 Size: 257 1069 NspI r_CATG'y Cuts at: 0 324 1133 1326 Size: 324 809 193 Fragments arranged by size: 809 324 193 PfoI T'CCnGG_A Cuts at: 0 233 1326 Size: 233 1093 PpuMI rG'GwC_Cy Cuts at: 0 1170 1326 Size: 1170 156 PshAI GACnn'nnGTC Cuts at: 0 957 1326 Size: 957 369 PstI C_TGCA'G Cuts at: 0 73 1326 Size: 73 1253 SacII CC_GC'GG Cuts at: 0 123 1326 Size: 123 1203 ScaI AGT'ACT Cuts at: 0 292 1326 Size: 292 1034 SfcI C'TryA_G Cuts at: 0 69 450 948 1326 Size: 69 381 498 378 Fragments arranged by size: 498 381 378 69 SmlI C'TyrA_G Cuts at: 0 598 955 1326 Size: 598 357 371 Fragments arranged by size: 598 371 357 SphI G_CATG'C Cuts at: 0 1133 1326 Size: 1133 193 StyI C'CwwG_G Cuts at: 0 299 1326 Size: 299 1027 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1290 1326 Size: 1290 36 TatI w'GTAC_w Cuts at: 0 290 1326 Size: 290 1036 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 972 1004 1060 1092 1326 Size: 972 32 56 32 234 Fragments arranged by size: 972 234 56 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 415 1326 Size: 415 911 XmnI GAAnn'nnTTC Cuts at: 0 713 1326 Size: 713 613 ZraI GAC'GTC Cuts at: 0 483 1326 Size: 483 843 Enzymes that do cut: AatII AccI Acc65I AcuI AfeI AleI AlwNI AvaI BaeI BanI BanII BbsI BcgI BciVI BclI BlpI BmrI BpmI Bpu10I BpuEI BsaBI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI BstAPI BstYI BtgI DraI EaeI EagI EarI EciI Eco57MI EcoO109I FspI HaeII Hin4I HindIII KpnI MmeI MslI MspA1I NotI NspI PfoI PpuMI PshAI PstI SacII ScaI SfcI SmlI SphI StyI TaqII TatI TstI XcmI XmnI ZraI Enzymes that do not cut: AarI AclI AflII AflIII AgeI AhdI AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BamHI BbeI BbvCI BfrBI BglI BglII Bme1580I BmgBI BmtI BplI BsaI BsaAI BsaWI BsaXI BsiWI BspEI BsrBI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraIII DrdI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspAI HincII HpaI KasI MfeI MluI MscI NaeI NarI NcoI NdeI NgoMIV NheI NruI NsiI PacI PasI PciI PflMI PmeI PmlI PpiI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI Tth111I XbaI XhoI XmaI