################################## # NO CUT ENZYMES ( 140 enzymes) # ################################## AatII Acc65I AccI AccII AciI AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BamHI BanI BbeI BbrPI BbvCI BciVI BclI BfrI BlnI BlpI Bpu1102I BsaHI BseRI BsgI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HpaI KasI KpnI KspI MfeI MluI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacII SalI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI SspBI SspI StuI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccIII 401 TCCGGA T^CCGGA AlwNI 775 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 668 CCTTAGG CC^T[ACGT]AGG ApaI 316 GGGCCC GGGCC^C Asp700I 405 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BbsI 1294 GTCTTC GAAGAC(2/6) BcgI 261 GCACGCACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 227 CCGGG CC^[GC]GG BglI 309 GCCAGGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 890 AGATCT A^GATCT BpuAI 1294 GTCTTC GAAGAC(2/6) BsaAI 764 TACGTG [CT]AC^GT[GA] BsaBI 286 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 401 TCCGGA [AT]^CCGG[AT] BseAI 401 TCCGGA T^CCGGA BsmI 770 GCATTC GAATGC(1/-1) BspEI 401 TCCGGA T^CCGGA BssSI 652 CACGAG CACGAG(-5/-1) BstXI 546 CCATGCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 668 CCTTAGG CC^T[ACGT]AGG DraI 1039 TTTAAA TTT^AAA EarI 47 CTCTTC CTCTTC(1/4) Eco81I 668 CCTTAGG CC^T[ACGT]AGG EcoRI 601 GAATTC G^AATTC HindIII 407 AAGCTT A^AGCTT Ksp632I 47 CTCTTC CTCTTC(1/4) MamI 286 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 401 TCCGGA T^CCGGA MspA1I 1145 CAGCTG C[AC]G^C[GT]G NciI 227 CCGGG CC^[GC]GG PleI 278 GAGTC GAGTC(4/5) PpuMI 1171 AGGACCT [GA]G^G[AT]CC[CT] PstI 25 CTGCAG CTGCA^G PvuII 1145 CAGCTG CAG^CTG SapI 46 GCTCTTC GCTCTTC(1/4) SbfI 24 CCTGCAGG CCTGCA^GG SmlI 127 CTTGAG C^T[CT][GA]AG Sse8387I 24 CCTGCAGG CCTGCA^GG XcmI 513 CCATGCTTTTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 405 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AspHI 44 GAGCTC G[AT]GC[AT]^C 497 GAGCTC BfaI 541 CTAG C^TAG 686 CTAG BpmI 41 CTGGAG CTGGAG(16/14) 250 CTGGAG BsaI 788 GAGACC GGTCTC(1/5) 936 GAGACC BsiHKAI 44 GAGCTC G[AT]GC[AT]^C 497 GAGCTC BsmFI 280 GTCCC GGGAC(10/14) 777 GTCCC BstF5I 384 GGATG GGATG(2/0) 579 GGATG DraII 315 GGGGCCC [GA]G^G[ACGT]CC[CT] 1171 AGGACCT DsaI 492 CCGTGG C^C[GA][CT]GG 878 CCGTGG EcoO109I 315 GGGGCCC [GA]G^G[ACGT]CC[CT] 1171 AGGACCT EcoT14I 1266 CCAAGG C^C[AT][AT]GG 1329 CCAAGG FokI 384 GGATG GGATG(9/13) 579 GGATG HapII 227 CCGG C^CGG 402 CCGG HincII 388 GTTGAC GT[CT]^[GA]AC 727 GTTGAC HindII 388 GTTGAC GT[CT]^[GA]AC 727 GTTGAC HpaII 227 CCGG C^CGG 402 CCGG MaeI 541 CTAG C^TAG 686 CTAG MspI 227 CCGG C^CGG 402 CCGG SacI 44 GAGCTC GAGCT^C 497 GAGCTC SfcI 25 CTGCAG C^T[GA][CT]AG 345 CTACAG StyI 1266 CCAAGG C^C[AT][AT]GG 1329 CCAAGG TaqI 270 TCGA T^CGA 590 TCGA TthHB8I 270 TCGA T^CGA 590 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 55 enzymes) # ######################################### AcsI AluI AlwI ApoI AvaII BalI BanII BbvI BmyI BsaJI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstNI BstYI Cac8I Cfr13I DdeI DpnII EaeI EcoRII Fnu4HI HaeIII HinfI HphI ItaI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MvaI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII