################################## # NO CUT ENZYMES ( 102 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AflII AgeI AscI AseI AsnI Asp700I Asp718I AspI AviII AvrII BbeI BbvCI BclI BfrI BglII BlnI BlpI Bpu1102I BsaWI BseAI BsiWI BspDI BspEI BspHI BsrBI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I CelII ClaI CpoI DraI DraIII DsaI EagI EclXI Eco52I EcoNI EcoO65I EcoRV EcoT14I EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KasI KpnI KspI MluI MroI MvnI NarI NcoI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI PvuI PvuII RcaI RsrII SacI SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I StyI SwaI Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AclI 326 AACGTT AA^CGTT AcyI 932 GGCGTC G[GA]^CG[CT]C AhdI 1654 GACTTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 1906 CCTAAGG CC^T[ACGT]AGG Aor51HI 1099 AGCGCT AGC^GCT AspEI 1654 GACTTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 118 CCCGGG C^[CT]CG[GA]G BamHI 1872 GGATCC G^GATCC BanI 1138 GGCACC G^G[CT][GA]CC BbrPI 778 CACGTG CAC^GTG BbsI 2149 GAAGAC GAAGAC(2/6) BcgI 29 GCACTGCTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1623 GGATAC GTATCC(6/5) BpuAI 2149 GAAGAC GAAGAC(2/6) BsaAI 778 CACGTG [CT]AC^GT[GA] BsaBI 274 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 932 GGCGTC G[GA]^CG[CT]C BsiEI 2214 CGACCG CG[GA][CT]^CG BsmI 716 GAATGC GAATGC(1/-1) BsoBI 118 CCCGGG C^[CT]CG[GA]G BspLU11I 1718 ACATGT A^CATGT BspMI 1106 ACCTGC ACCTGC(4/8) BsrDI 384 GCAATG GCAATG(2/0) BstXI 1337 CCAAAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 1906 CCTAAGG CC^T[ACGT]AGG DrdI 1558 GACCTTCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 1654 GACTTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1099 AGCGCT AGC^GCT Eco81I 1906 CCTAAGG CC^T[ACGT]AGG EcoRI 1730 GAATTC G^AATTC HaeII 1099 AGCGCT [GA]GCGC^[CT] Hin1I 932 GGCGTC G[GA]^CG[CT]C MamI 274 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NaeI 842 GCCGGC GCC^GGC NdeI 1798 CATATG CA^TATG NgoMIV 842 GCCGGC G^CCGGC PflMI 1364 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 778 CACGTG CAC^GTG PmlI 778 CACGTG CAC^GTG Psp1406I 326 AACGTT AA^CGTT PstI 22 CTGCAG CTGCA^G SapI 8 GAAGAGC GCTCTTC(1/4) SexAI 1226 ACCTGGT A^CC[AT]GGT SmaI 118 CCCGGG CCC^GGG SspI 1541 AATATT AAT^ATT StuI 1011 AGGCCT AGG^CCT Van91I 1364 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 118 CCCGGG C^CCGGG XmaI 118 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 38 enzymes) # ################################### AcsI 1463 AAATTC [GA]^AATT[CT] 1730 GAATTC Alw44I 27 GTGCAC G^TGCAC 59 GTGCAC AlwNI 1222 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG 1365 CAGGACCTG ApaI 123 GGGCCC GGGCC^C 804 GGGCCC ApaLI 27 GTGCAC G^TGCAC 59 GTGCAC ApoI 1463 AAATTC [GA]^AATT[CT] 1730 GAATTC BalI 1052 TGGCCA TGG^CCA 1259 TGGCCA BglI 837 GCCTGGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 850 GCCCAGAAGGC BsaI 1556 GAGACC GGTCTC(1/5) 233 GGTCTC BsiYI 1364 CCAGGACCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1986 CCCTTCCCAGG BslI 1364 CCAGGACCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1986 CCCTTCCCAGG BsmBI 1126 GAGACG CGTCTC(1/5) 2210 GAGACG Bsp1407I 1518 TGTACA T^GTACA 1817 TGTACA BsrFI 842 GCCGGC [GA]^CCGG[CT] 2216 ACCGGC BsrGI 1518 TGTACA T^GTACA 1817 TGTACA BssSI 1095 CACGAG CACGAG(-5/-1) 1811 CTCGTG BstYI 113 AGATCC [GA]^GATC[CT] 1872 GGATCC CfoI 50 GCGC GCG^C 1100 GCGC Cfr10I 842 GCCGGC [GA]^CCGG[CT] 2216 ACCGGC FauI 1254 CCCGC CCCGC(4/6) 674 GCGGG HgaI 1128 GACGC GACGC(5/10) 933 GCGTC HhaI 50 GCGC GCG^C 1100 GCGC HinP1I 50 GCGC G^CGC 1100 GCGC MfeI 758 CAATTG C^AATTG 1535 CAATTG MflI 113 AGATCC [GA]^GATC[CT] 1872 GGATCC MluNI 1052 TGGCCA TGG^CCA 1259 TGGCCA MscI 1052 TGGCCA TGG^CCA 1259 TGGCCA MslI 711 CATAAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1917 CACCCTGGTG MunI 758 CAATTG C^AATTG 1535 CAATTG NheI 553 GCTAGC G^CTAGC 846 GCTAGC NspI 1515 GCATGT [GA]CATG^[CT] 1718 ACATGT PpuMI 1366 AGGACCT [GA]G^G[AT]CC[CT] 1777 GGGACCT SfcI 22 CTGCAG C^T[GA][CT]AG 1688 CTATAG SspBI 1518 TGTACA T^GTACA 1817 TGTACA TaqI 237 TCGA T^CGA 1311 TCGA TthHB8I 237 TCGA T^CGA 1311 TCGA XcmI 1198 CCAAATCTCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1337 CCAAAGCTCTGGTGG XhoII 113 AGATCC [GA]^GATC[CT] 1872 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 69 enzymes) # ######################################### AciI AfaI AflIII AluI AlwI AspHI AvaII BanII BbvI BcnI BfaI BmyI BpmI BsaJI BseRI BsgI BsiHKAI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI TfiI Tru9I TseI Tsp45I Tsp509I TspRI