(Circular) (Six-base) MAPSORT of: pFN21AE2080.seq Check: 1306 from: 1 to: 6964 pFN21AE2080 6964 bp With 182 enzymes: SgfI * August 16, 2010 14:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4774 278 Size: 53 83 186 936 3238 2468 Fragments arranged by size: 3238 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4307 1937 Size: 2370 4594 Acc65I G'GTAC_C Cuts at: 4285 4285 Size: 6964 AclI AA'CG_TT Cuts at: 1563 2332 5091 5464 1563 Size: 769 2759 373 3063 Fragments arranged by size: 3063 2759 769 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4173 5027 6411 6679 1632 Size: 2541 854 1384 268 1917 Fragments arranged by size: 2541 1917 1384 854 268 AfeI AGC'GCT Cuts at: 3106 3106 Size: 6964 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6767 AflIII A'CryG_T Cuts at: 1203 2782 3723 3825 5863 6840 1203 Size: 1579 941 102 2038 977 1327 Fragments arranged by size: 2038 1579 1327 977 941 102 AgeI A'CCGG_T Cuts at: 5795 5795 Size: 6964 AhdI GACnn_n'nnGTC Cuts at: 3664 5693 3664 Size: 2029 4935 AleI CACnn'nnGTG Cuts at: 1166 3926 1166 Size: 2760 4204 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2052 2738 1184 Size: 868 686 5410 Fragments arranged by size: 5410 868 686 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4118 4150 4118 Size: 32 6932 AlwNI CAG_nnn'CTG Cuts at: 3232 3375 4707 6279 3232 Size: 143 1332 1572 3917 Fragments arranged by size: 3917 1572 1332 143 ApaI G_GGCC'C Cuts at: 2132 2813 2132 Size: 681 6283 ApaLI G'TGCA_C Cuts at: 2032 2064 5021 6177 2032 Size: 32 2957 1156 2819 Fragments arranged by size: 2957 2819 1156 32 ApoI r'AATT_y Cuts at: 1493 3468 3735 4273 4499 4651 6760 1493 Size: 1975 267 538 226 152 2109 1697 Fragments arranged by size: 2109 1975 1697 538 267 226 152 AseI AT'TA_AT Cuts at: 160 1032 5518 160 Size: 872 4486 1606 Fragments arranged by size: 4486 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6964 AvaI C'yCGr_G Cuts at: 1870 1943 2123 4289 1870 Size: 73 180 2166 4545 Fragments arranged by size: 4545 2166 180 73 BamHI G'GATC_C Cuts at: 3877 4294 4661 3877 Size: 417 367 6180 Fragments arranged by size: 6180 417 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3143 4285 5740 618 Size: 325 217 627 1356 1142 1455 1842 Fragments arranged by size: 1842 1455 1356 1142 627 325 217 BanII G_rGCy'C Cuts at: 1391 2132 2813 3432 3439 3991 4283 1391 Size: 741 681 619 7 552 292 4072 Fragments arranged by size: 4072 741 681 619 552 292 7 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4161 928 Size: 971 2262 3731 Fragments arranged by size: 3731 2262 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2023 2057 5155 5189 1903 Size: 34 86 34 3098 34 3678 Fragments arranged by size: 3678 3098 86 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3622 4856 6066 3622 Size: 1234 1210 4520 Fragments arranged by size: 4520 1234 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6964 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2848 2861 5575 Size: 107 122 71 631 605 1176 13 2714 Cuts at: 5575 136 Size: 1525 Fragments arranged by size: 2714 1525 1176 631 605 122 107 71 13 BglII A'GATC_T Cuts at: 6959 6959 Size: 6964 BlpI GC'TnA_GC Cuts at: 4381 4381 Size: 6964 Bme1580I G_kGCm'C Cuts at: 1790 2036 2068 2132 2813 5025 6181 1790 Size: 246 32 64 681 2212 1156 2573 Fragments arranged by size: 2573 2212 1156 681 246 64 32 BmgBI CAC'GTC Cuts at: 1318 3828 1318 Size: 2510 4454 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4695 5653 442 Size: 416 53 83 399 24 3278 958 1753 Fragments arranged by size: 3278 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2562 2855 1056 Size: 1506 293 5165 Fragments arranged by size: 5165 1506 293 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3061 3061 Size: 6964 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2041 3397 3536 5624 1239 Size: 802 1356 139 2088 2579 Fragments arranged by size: 2579 2088 1356 802 139 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5089 5954 6252 5089 Size: 865 298 5801 Fragments arranged by size: 5801 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2244 3555 5627 882 Size: 617 745 1311 2072 2219 Fragments arranged by size: 2219 2072 1311 745 617 BsaAI yAC'GTr Cuts at: 493 1204 2785 4755 493 Size: 711 1581 1970 2702 Fragments arranged by size: 2702 1970 1581 711 BsaBI GATnn'nnATC Cuts at: 2283 6958 2283 Size: 4675 2289 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2938 4771 5153 5788 Size: 53 83 186 936 1405 1833 382 635 Cuts at: 5788 275 Size: 1451 Fragments arranged by size: 1833 1451 1405 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5397 5795 6069 6216 6627 5397 Size: 398 274 147 411 5734 Fragments arranged by size: 5734 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2975 3005 1178 Size: 30 165 30 1572 30 5137 Fragments arranged by size: 5137 1572 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2171 3037 3394 3573 3902 3995 1186 Size: 985 866 357 179 329 93 4155 Fragments arranged by size: 4155 985 866 357 329 179 93 BseYI C'CCAG_C Cuts at: 2161 2805 3009 3941 4137 6167 6663 2161 Size: 644 204 932 196 2030 496 2462 Fragments arranged by size: 2462 2030 932 644 496 204 196 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2661 3098 3109 3141 1163 Size: 573 925 437 11 32 4986 Fragments arranged by size: 4986 925 573 437 32 11 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4222 4414 5175 6203 1540 Size: 275 186 2221 192 761 1028 2301 Fragments arranged by size: 2301 2221 1028 761 275 192 186 BsiHKAI G_wGCw'C Cuts at: 2036 2068 3047 3609 5025 5110 6181 2036 Size: 32 979 562 1416 85 1071 2819 Fragments arranged by size: 2819 1416 1071 979 562 85 32 BsmI GAATG_Cn' Cuts at: 2727 4475 4568 2727 Size: 1748 93 5123 Fragments arranged by size: 5123 1748 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3125 4209 3125 Size: 1084 5880 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2036 2068 2132 2813 3047 3432 3439 Size: 399 246 32 64 681 234 385 7 Cuts at: 3439 3609 3991 4283 5025 5110 6181 1391 Size: 170 382 292 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 681 399 385 382 292 246 234 170 85 64 32 7 BspEI T'CCGG_A Cuts at: 6627 6627 Size: 6964 BspHI T'CATG_A Cuts at: 4853 6583 4853 Size: 1730 5234 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3120 4319 844 Size: 2276 1199 3489 Fragments arranged by size: 3489 2276 1199 BsrBI CCG'CTC Cuts at: 1394 1823 4851 1394 Size: 429 3028 3507 Fragments arranged by size: 3507 3028 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2396 5459 5633 65 Size: 1183 1148 3063 174 1396 Fragments arranged by size: 3063 1396 1183 1148 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2847 4221 5608 5795 1700 Size: 110 1037 1374 1387 187 2869 Fragments arranged by size: 2869 1387 1374 1037 187 110 BsrGI T'GTAC_A Cuts at: 96 3523 3822 96 Size: 3427 299 3238 Fragments arranged by size: 3427 3238 299 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6964 BssSI C'ACGA_G Cuts at: 3100 3816 5024 6036 3100 Size: 716 1208 1012 4028 Fragments arranged by size: 4028 1208 1012 716 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6964 BstBI TT'CG_AA Cuts at: 5768 5768 Size: 6964 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6964 BstXI CCAn_nnnn'nTGG Cuts at: 3349 3349 Size: 6964 BstYI r'GATC_y Cuts at: 1972 2118 3877 4294 4661 5046 5063 6515 6601 Size: 146 1759 417 367 385 17 1452 86 Cuts at: 6601 6613 6959 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1759 1452 417 385 367 346 146 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3912 3912 Size: 6964 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5473 Fragments arranged by size: 5473 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2134 487 Size: 683 56 908 5317 Fragments arranged by size: 5317 908 683 56 BtsI GCAGTG_nn' Cuts at: 2034 2036 3007 4480 5274 5294 2034 Size: 2 971 1473 794 20 3704 Fragments arranged by size: 3704 1473 971 794 20 2 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6964 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6929 DraI TTT'AAA Cuts at: 4268 4268 Size: 6964 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3569 5971 809 Size: 507 2253 2402 1802 Fragments arranged by size: 2402 2253 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2145 2845 3057 Size: 54 1425 50 275 18 315 700 212 Cuts at: 3057 3264 4411 5300 6929 8 Size: 207 1147 889 1629 43 Fragments arranged by size: 1629 1425 1147 889 700 315 275 212 207 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4411 1537 Size: 275 2599 4090 Fragments arranged by size: 4090 2599 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2008 2369 3550 3793 3850 4894 1353 Size: 655 361 1181 243 57 1044 3423 Fragments arranged by size: 3423 1181 1044 655 361 243 57 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5551 5925 6071 387 Size: 1064 138 3962 374 146 1280 Fragments arranged by size: 3962 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2041 3397 3536 4173 5027 5624 6411 Size: 393 409 1356 139 637 854 597 787 Cuts at: 6411 6679 1239 Size: 268 1524 Fragments arranged by size: 1524 1356 854 787 637 597 409 393 268 139 EcoO109I rG'GnC_Cy Cuts at: 2128 2809 2810 2840 3372 3783 2128 Size: 681 1 30 532 411 5309 Fragments arranged by size: 5309 681 532 411 30 1 EcoRI G'AATT_C Cuts at: 3735 4273 4651 3735 Size: 538 378 6048 Fragments arranged by size: 6048 538 378 FspI TGC'GCA Cuts at: 5470 5470 Size: 6964 HaeII r_GCGC'y Cuts at: 1678 3108 6111 1678 Size: 1430 3003 2531 Fragments arranged by size: 3003 2531 1430 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2475 2507 3444 3476 3650 Size: 32 135 32 903 32 937 32 174 Cuts at: 3650 3682 3775 3807 3862 3894 5605 5637 5679 Size: 32 93 32 55 32 1711 32 42 Cuts at: 5679 5711 1373 Size: 32 2626 Fragments arranged by size: 2626 1711 937 903 174 135 93 55 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4308 4554 669 Size: 988 281 2370 246 3079 Fragments arranged by size: 3079 2370 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6964 HpaI GTT'AAC Cuts at: 4554 4554 Size: 6964 KpnI G_GTAC'C Cuts at: 4289 4289 Size: 6964 MfeI C'AATT_G Cuts at: 2763 3540 4563 2763 Size: 777 1023 5164 Fragments arranged by size: 5164 1023 777 MluI A'CGCG_T Cuts at: 6840 6840 Size: 6964 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2665 2689 3497 3957 6078 6262 1305 Size: 1360 24 808 460 2121 184 2007 Fragments arranged by size: 2121 2007 1360 808 460 184 24 MscI TGG'CCA Cuts at: 10 64 1489 1832 3059 3266 10 Size: 54 1425 343 1227 207 3708 Fragments arranged by size: 3708 1425 1227 343 207 54 MslI CAynn'nnrTG Cuts at: 518 1166 2720 3926 4922 5281 5440 518 Size: 648 1554 1206 996 359 159 2042 Fragments arranged by size: 2042 1554 1206 996 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2114 3075 3262 4380 5057 6205 6450 1606 Size: 508 961 187 1118 677 1148 245 2120 Fragments arranged by size: 2120 1148 1118 961 677 508 245 187 NaeI GCC'GGC Cuts at: 2849 2849 Size: 6964 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5473 Fragments arranged by size: 5473 939 552 NdeI CA'TA_TG Cuts at: 387 3804 387 Size: 3417 3547 NgoMIV G'CCGG_C Cuts at: 2847 2847 Size: 6964 NheI G'CTAG_C Cuts at: 1052 2558 2851 1052 Size: 1506 293 5165 Fragments arranged by size: 5165 1506 293 NotI GC'GGCC_GC Cuts at: 4411 4411 Size: 6964 NspI r_CATG'y Cuts at: 1132 1229 3524 3727 4322 5867 1132 Size: 97 2295 203 595 1545 2229 Fragments arranged by size: 2295 2229 1545 595 203 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6964 PciI A'CATG_T Cuts at: 3723 5863 3723 Size: 2140 4824 PflMI CCAn_nnn'nTGG Cuts at: 1108 3375 1108 Size: 2267 4697 PfoI T'CCnGG_A Cuts at: 2776 2776 Size: 6964 PmeI GTTT'AAAC Cuts at: 4268 4268 Size: 6964 PmlI CAC'GTG Cuts at: 2785 2785 Size: 6964 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5033 5065 6546 6578 5033 Size: 32 1481 32 5419 Fragments arranged by size: 5419 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3372 3783 3372 Size: 411 6553 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6964 PsiI TTA'TAA Cuts at: 4191 4534 4191 Size: 343 6621 PspOMI G'GGCC_C Cuts at: 2128 2809 2128 Size: 681 6283 PstI C_TGCA'G Cuts at: 830 2031 4316 830 Size: 1201 2285 3478 Fragments arranged by size: 3478 2285 1201 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6964 SalI G'TCGA_C Cuts at: 1936 4306 1936 Size: 2370 4594 SapI GCTCTTCn'nnn_ Cuts at: 2008 2008 Size: 6964 SbfI CC_TGCA'GG Cuts at: 4316 4316 Size: 6964 ScaI AGT'ACT Cuts at: 5212 6924 5212 Size: 1712 5252 SexAI A'CCwGG_T Cuts at: 3231 3231 Size: 6964 SfcI C'TryA_G Cuts at: 826 1045 2027 3693 4312 5447 6128 6319 826 Size: 219 982 1666 619 1135 681 191 1471 Fragments arranged by size: 1666 1471 1135 982 681 619 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6964 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6964 SmaI CCC'GGG Cuts at: 1872 2125 4291 1872 Size: 253 2166 4545 Fragments arranged by size: 4545 2166 253 SmlI C'TyrA_G Cuts at: 820 1017 1943 5068 5969 6231 820 Size: 197 926 3125 901 262 1553 Fragments arranged by size: 3125 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4755 493 Size: 4262 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6964 SphI G_CATG'C Cuts at: 1132 4322 1132 Size: 3190 3774 SspI AAT'ATT Cuts at: 5 52 3548 4888 5 Size: 47 3496 1340 2081 Fragments arranged by size: 3496 2081 1340 47 StuI AGG'CCT Cuts at: 1517 3018 1517 Size: 1501 5463 StyI C'CwwG_G Cuts at: 513 1065 2004 4403 513 Size: 552 939 2399 3074 Fragments arranged by size: 3074 2399 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4991 5008 5161 5346 5791 325 Size: 1255 3411 17 153 185 445 1498 Fragments arranged by size: 3411 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3523 3822 5210 Size: 274 80 33 51 1443 1546 299 1388 Cuts at: 5210 6922 96 Size: 1712 138 Fragments arranged by size: 1712 1546 1443 1388 299 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2623 2655 3741 3773 6924 6956 2623 Size: 32 1086 32 3151 32 2631 Fragments arranged by size: 3151 2631 1086 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6964 XbaI T'CTAG_A Cuts at: 4300 4300 Size: 6964 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3210 3349 3210 Size: 139 6825 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6964 XmaI C'CCGG_G Cuts at: 1870 2123 4289 1870 Size: 253 2166 4545 Fragments arranged by size: 4545 2166 253 XmnI GAAnn'nnTTC Cuts at: 5093 5093 Size: 6964 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4772 276 Size: 53 83 186 936 3238 2468 Fragments arranged by size: 3238 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PspXI PsrI PvuII RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI