(Linear) (Six-base) MAPSORT of: KIEE2080.seq Check: 4316 from: 1 to: 2259 KIEE2080.orf With 182 enzymes: SgfI * July 15, 2010 18:54 .. AclI AA'CG_TT Cuts at: 0 327 2259 Size: 327 1932 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2168 2259 Size: 2168 91 AfeI AGC'GCT Cuts at: 0 1101 2259 Size: 1101 1158 AflIII A'CryG_T Cuts at: 0 777 1718 1820 2259 Size: 777 941 102 439 Fragments arranged by size: 941 777 439 102 AhdI GACnn_n'nnGTC Cuts at: 0 1659 2259 Size: 1659 600 AleI CACnn'nnGTG Cuts at: 0 1921 2259 Size: 1921 338 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 47 733 2259 Size: 47 686 1526 Fragments arranged by size: 1526 686 47 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2113 2145 2259 Size: 2113 32 114 Fragments arranged by size: 2113 114 32 AlwNI CAG_nnn'CTG Cuts at: 0 1227 1370 2259 Size: 1227 143 889 Fragments arranged by size: 1227 889 143 ApaI G_GGCC'C Cuts at: 0 127 808 2259 Size: 127 681 1451 Fragments arranged by size: 1451 681 127 ApaLI G'TGCA_C Cuts at: 0 27 59 2259 Size: 27 32 2200 Fragments arranged by size: 2200 32 27 ApoI r'AATT_y Cuts at: 0 1463 1730 2259 Size: 1463 267 529 Fragments arranged by size: 1463 529 267 AvaI C'yCGr_G Cuts at: 0 118 2259 Size: 118 2141 BamHI G'GATC_C Cuts at: 0 1872 2259 Size: 1872 387 BanI G'GyrC_C Cuts at: 0 1138 2259 Size: 1138 1121 BanII G_rGCy'C Cuts at: 0 127 808 1427 1434 1986 2259 Size: 127 681 619 7 552 273 Fragments arranged by size: 681 619 552 273 127 7 BbsI GAAGACnn'nnnn_ Cuts at: 0 2156 2259 Size: 2156 103 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 18 52 2259 Size: 18 34 2207 Fragments arranged by size: 2207 34 18 BciVI GTATCCnnnnn_n' Cuts at: 0 1617 2259 Size: 1617 642 BglI GCCn_nnn'nGGC Cuts at: 0 843 856 2259 Size: 843 13 1403 Fragments arranged by size: 1403 843 13 Bme1580I G_kGCm'C Cuts at: 0 31 63 127 808 2259 Size: 31 32 64 681 1451 Fragments arranged by size: 1451 681 64 32 31 BmgBI CAC'GTC Cuts at: 0 1823 2259 Size: 1823 436 BmtI G_CTAG'C Cuts at: 0 557 850 2259 Size: 557 293 1409 Fragments arranged by size: 1409 557 293 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1056 2259 Size: 1056 1203 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 36 1392 1531 2259 Size: 36 1356 139 728 Fragments arranged by size: 1356 728 139 36 BsaI GGTCTCn'nnnn_ Cuts at: 0 239 1550 2259 Size: 239 1311 709 Fragments arranged by size: 1311 709 239 BsaAI yAC'GTr Cuts at: 0 780 2259 Size: 780 1479 BsaBI GATnn'nnATC Cuts at: 0 278 2259 Size: 278 1981 BsaHI Gr'CG_yC Cuts at: 0 933 2259 Size: 933 1326 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 970 1000 2259 Size: 970 30 1259 Fragments arranged by size: 1259 970 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 166 1032 1389 1568 1897 1990 2259 Size: 166 866 357 179 329 93 269 Fragments arranged by size: 866 357 329 269 179 166 93 BseYI C'CCAG_C Cuts at: 0 156 800 1004 1936 2132 2259 Size: 156 644 204 932 196 127 Fragments arranged by size: 932 644 204 196 156 127 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 656 1093 1104 1136 2259 Size: 656 437 11 32 1123 Fragments arranged by size: 1123 656 437 32 11 BsiEI CG_ry'CG Cuts at: 0 2217 2259 Size: 2217 42 BsiHKAI G_wGCw'C Cuts at: 0 31 63 1042 1604 2259 Size: 31 32 979 562 655 Fragments arranged by size: 979 655 562 32 31 BsmI GAATG_Cn' Cuts at: 0 722 2259 Size: 722 1537 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1120 2204 2259 Size: 1120 1084 55 Fragments arranged by size: 1120 1084 55 Bsp1286I G_dGCh'C Cuts at: 0 31 63 127 808 1042 1427 1434 1604 Size: 31 32 64 681 234 385 7 170 Cuts at: 1604 1986 2259 Size: 382 273 Fragments arranged by size: 681 385 382 273 234 170 64 32 31 7 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1115 2259 Size: 1115 1144 BsrDI GCAATG_nn' Cuts at: 0 391 2259 Size: 391 1868 BsrFI r'CCGG_y Cuts at: 0 842 2216 2259 Size: 842 1374 43 Fragments arranged by size: 1374 842 43 BsrGI T'GTAC_A Cuts at: 0 1518 1817 2259 Size: 1518 299 442 Fragments arranged by size: 1518 442 299 BssSI C'ACGA_G Cuts at: 0 1095 1811 2259 Size: 1095 716 448 Fragments arranged by size: 1095 716 448 BstXI CCAn_nnnn'nTGG Cuts at: 0 1344 2259 Size: 1344 915 BstYI r'GATC_y Cuts at: 0 113 1872 2259 Size: 113 1759 387 Fragments arranged by size: 1759 387 113 Bsu36I CC'TnA_GG Cuts at: 0 1907 2259 Size: 1907 352 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 129 2259 Size: 129 2130 BtsI GCAGTG_nn' Cuts at: 0 29 31 1002 2259 Size: 29 2 971 1257 Fragments arranged by size: 1257 971 29 2 DrdI GACnn_nn'nnGTC Cuts at: 0 1564 2259 Size: 1564 695 EaeI y'GGCC_r Cuts at: 0 140 840 1052 1259 2259 Size: 140 700 212 207 1000 Fragments arranged by size: 1000 700 212 207 140 EarI CTCTTCn'nnn_ Cuts at: 0 3 364 1545 1788 1845 2259 Size: 3 361 1181 243 57 414 Fragments arranged by size: 1181 414 361 243 57 3 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 36 1392 1531 2168 2259 Size: 36 1356 139 637 91 Fragments arranged by size: 1356 637 139 91 36 EcoO109I rG'GnC_Cy Cuts at: 0 123 804 805 835 1367 1778 2259 Size: 123 681 1 30 532 411 481 Fragments arranged by size: 681 532 481 411 123 30 1 EcoRI G'AATT_C Cuts at: 0 1730 2259 Size: 1730 529 HaeII r_GCGC'y Cuts at: 0 1103 2259 Size: 1103 1156 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 470 502 1439 1471 1645 1677 1770 1802 Size: 470 32 937 32 174 32 93 32 Cuts at: 1802 1857 1889 2259 Size: 55 32 370 Fragments arranged by size: 937 470 370 174 93 55 32 32 32 32 32 MfeI C'AATT_G Cuts at: 0 758 1535 2259 Size: 758 777 724 Fragments arranged by size: 777 758 724 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 660 684 1492 1952 2259 Size: 660 24 808 460 307 Fragments arranged by size: 808 660 460 307 24 MscI TGG'CCA Cuts at: 0 1054 1261 2259 Size: 1054 207 998 Fragments arranged by size: 1054 998 207 MslI CAynn'nnrTG Cuts at: 0 715 1921 2259 Size: 715 1206 338 Fragments arranged by size: 1206 715 338 MspA1I CmG'CkG Cuts at: 0 109 1070 1257 2259 Size: 109 961 187 1002 Fragments arranged by size: 1002 961 187 109 NaeI GCC'GGC Cuts at: 0 844 2259 Size: 844 1415 NdeI CA'TA_TG Cuts at: 0 1799 2259 Size: 1799 460 NgoMIV G'CCGG_C Cuts at: 0 842 2259 Size: 842 1417 NheI G'CTAG_C Cuts at: 0 553 846 2259 Size: 553 293 1413 Fragments arranged by size: 1413 553 293 NspI r_CATG'y Cuts at: 0 1519 1722 2259 Size: 1519 203 537 Fragments arranged by size: 1519 537 203 PciI A'CATG_T Cuts at: 0 1718 2259 Size: 1718 541 PflMI CCAn_nnn'nTGG Cuts at: 0 1370 2259 Size: 1370 889 PfoI T'CCnGG_A Cuts at: 0 771 2259 Size: 771 1488 PmlI CAC'GTG Cuts at: 0 780 2259 Size: 780 1479 PpuMI rG'GwC_Cy Cuts at: 0 1367 1778 2259 Size: 1367 411 481 Fragments arranged by size: 1367 481 411 PsiI TTA'TAA Cuts at: 0 2186 2259 Size: 2186 73 PspOMI G'GGCC_C Cuts at: 0 123 804 2259 Size: 123 681 1455 Fragments arranged by size: 1455 681 123 PstI C_TGCA'G Cuts at: 0 26 2259 Size: 26 2233 SapI GCTCTTCn'nnn_ Cuts at: 0 3 2259 Size: 3 2256 SexAI A'CCwGG_T Cuts at: 0 1226 2259 Size: 1226 1033 SfcI C'TryA_G Cuts at: 0 22 1688 2259 Size: 22 1666 571 Fragments arranged by size: 1666 571 22 SmaI CCC'GGG Cuts at: 0 120 2259 Size: 120 2139 SspI AAT'ATT Cuts at: 0 1543 2259 Size: 1543 716 StuI AGG'CCT Cuts at: 0 1013 2259 Size: 1013 1246 TatI w'GTAC_w Cuts at: 0 1518 1817 2259 Size: 1518 299 442 Fragments arranged by size: 1518 442 299 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 618 650 1736 1768 2259 Size: 618 32 1086 32 491 Fragments arranged by size: 1086 618 491 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1205 1344 2259 Size: 1205 139 915 Fragments arranged by size: 1205 915 139 XmaI C'CCGG_G Cuts at: 0 118 2259 Size: 118 2141 Enzymes that do cut: AclI AcuI AfeI AflIII AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII BbsI BcgI BciVI BglI Bme1580I BmgBI BmtI BplI BpmI BsaI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrDI BsrFI BsrGI BssSI BstXI BstYI Bsu36I BtgZI BtsI DrdI EaeI EarI Eco57MI EcoO109I EcoRI HaeII Hin4I MfeI MmeI MscI MslI MspA1I NaeI NdeI NgoMIV NheI NspI PciI PflMI PfoI PmlI PpuMI PsiI PspOMI PstI SapI SexAI SfcI SmaI SspI StuI TatI TstI XcmI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AflII AgeI AscI AseI AsiSI AvrII BaeI BbeI BbvCI BclI BfrBI BglII BlpI BmrI Bpu10I BpuEI BsaWI BsiWI BspEI BspHI BsrBI BssHII BstAPI BstBI BstEII BstZ17I BtgI ClaI CspCI DraI DraIII EagI EciI EcoICRI EcoNI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KasI KpnI MluI NarI NcoI NotI NruI NsiI PacI PasI PmeI PpiI PshAI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SanDI SbfI ScaI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI StyI SwaI TaqII Tth111I XbaI XhoI XmnI ZraI