################################## # NO CUT ENZYMES ( 115 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AgeI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspHI AvrII BalI BamHI BanII BbeI BbrPI BbvCI BciVI BfrI BlnI BlpI BmyI Bpu1102I BsaAI BsaBI BsaHI BseAI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspDI BspEI BspLU11I BsrBI Bst1107I BstBI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DrdI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV EcoT22I FseI HgaI Hin1I HincII HindII HpaI KasI KpnI MamI MfeI MluI MluNI MroI MscI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PflMI PinAI PmaCI PmeI PmlI Psp1406I PvuI PvuII RsrII SacI SalI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SwaI TfiI Van91I XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AflIII 737 ACGTGT A^C[GA][CT]GT AhdI 928 GACTACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspEI 928 GACTACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 1161 CTCGAG C^[CT]CG[GA]G AviII 1004 TGCGCA TGC^GCA BanI 1289 GGCACC G^G[CT][GA]CC BbsI 440 GAAGAC GAAGAC(2/6) BcgI 734 GCAACGTGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 579 TGATCA T^GATCA BglI 26 GCCCCGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 138 AGATCT A^GATCT BpuAI 440 GAAGAC GAAGAC(2/6) BsaWI 261 ACCGGA [AT]^CCGG[AT] BsgI 1276 CTGCAC GTGCAG(16/14) BsmI 333 GAATGC GAATGC(1/-1) BsoBI 1161 CTCGAG C^[CT]CG[GA]G Bsp1407I 1334 TGTACA T^GTACA BsrDI 1308 CATTGC GCAATG(2/0) BsrFI 972 GCCGGT [GA]^CCGG[CT] BsrGI 1334 TGTACA T^GTACA BssHII 1182 GCGCGC G^CGCGC BssSI 994 CACGAG CACGAG(-5/-1) BstAPI 1010 GCAAGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 320 GGTTACC G^GT[ACGT]ACC BstPI 320 GGTTACC G^GT[ACGT]ACC Cfr10I 972 GCCGGT [GA]^CCGG[CT] DraIII 1291 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 92 TGGCCG [CT]^GGCC[GA] Eam1105I 928 GACTACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 826 GAAGAG CTCTTC(1/4) EcoO65I 320 GGTTACC G^GT[ACGT]ACC EcoT14I 494 CCATGG C^C[AT][AT]GG FbaI 579 TGATCA T^GATCA FspI 1004 TGCGCA TGC^GCA HindIII 648 AAGCTT A^AGCTT HinfI 516 GAGTC G^A[ACGT]TC HphI 939 GGTGA GGTGA(8/7) Ksp632I 826 GAAGAG CTCTTC(1/4) KspI 29 CCGCGG CCGC^GG MslI 1308 CATTGCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 29 CCGCGG C[AC]G^C[GT]G NcoI 494 CCATGG C^CATGG NspI 782 ACATGC [GA]CATG^[CT] PaeR7I 1161 CTCGAG C^TCGAG PleI 516 GAGTC GAGTC(4/5) PshAI 693 GACCGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacII 29 CCGCGG CCGC^GG SapI 826 GAAGAGC GCTCTTC(1/4) SexAI 986 ACCAGGT A^CC[AT]GGT SfaNI 1040 GCATC GCATC(5/9) SmlI 1161 CTCGAG C^T[CT][GA]AG SspBI 1334 TGTACA T^GTACA SspI 483 AATATT AAT^ATT StuI 1157 AGGCCT AGG^CCT StyI 494 CCATGG C^C[AT][AT]GG XbaI 285 TCTAGA T^CTAGA XcmI 494 CCATGGATCTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 1161 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AccI 354 GTCTAC GT^[AC][GT]AC 745 GTCTAC AseI 304 ATTAAT AT^TAAT 616 ATTAAT AsnI 304 ATTAAT AT^TAAT 616 ATTAAT AspI 1092 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 1300 GACAATGTC BcnI 1094 CCGGG CC^[GC]GG 1199 CCCGG BfaI 218 CTAG C^TAG 286 CTAG BpmI 134 CTGGAG CTGGAG(16/14) 1153 CTGGAG BsaI 449 GAGACC GGTCTC(1/5) 979 GAGACC BseRI 789 CTCCTC GAGGAG(10/8) 685 GAGGAG BspMI 1001 ACCTGC ACCTGC(4/8) 63 GCAGGT BsrI 48 CCAGT ACTGG(1/-1) 729 CCAGT HaeII 468 GGCGCT [GA]GCGC^[CT] 1081 AGCGCC MaeI 218 CTAG C^TAG 286 CTAG MaeII 89 ACGT A^CGT 737 ACGT NciI 1094 CCGGG CC^[GC]GG 1199 CCCGG PflFI 1092 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 1300 GACAATGTC PshBI 304 ATTAAT AT^TAAT 616 ATTAAT PstI 599 CTGCAG CTGCA^G 707 CTGCAG Tsp45I 779 GTGAC ^GT[GC]AC 1147 GTGAC Tth111I 1092 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 1300 GACAATGTC ######################################### # 3 OR MORE CUT ENZYMES ( 65 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI ApoI AvaII BbvI BsaJI BsiYI BslI BsmAI BsmFI BspHI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII ItaI MaeIII MboI MboII MflI MnlI MseI MspI MvaI MvnI MwoI NdeII NlaIII NlaIV PpuMI RcaI RsaI Sau3AI Sau96I ScrFI SfcI TaqI Tru9I TseI Tsp509I TspRI TthHB8I XhoII