(Circular) (Six-base) MAPSORT of: pFN21AE2030.seq Check: 3387 from: 1 to: 6565 pFN21AE2030 6565 bp With 182 enzymes: SgfI * August 16, 2010 14:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4375 278 Size: 53 83 186 936 2839 2468 Fragments arranged by size: 2839 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2487 3908 1937 Size: 550 1421 4594 Fragments arranged by size: 4594 1421 550 Acc65I G'GTAC_C Cuts at: 3886 3886 Size: 6565 AclI AA'CG_TT Cuts at: 1563 4692 5065 1563 Size: 3129 373 3063 Fragments arranged by size: 3129 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3160 3615 4628 6012 6280 1632 Size: 1528 455 1013 1384 268 1917 Fragments arranged by size: 1917 1528 1384 1013 455 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6368 AflIII A'CryG_T Cuts at: 1203 5464 6441 1203 Size: 4261 977 1327 Fragments arranged by size: 4261 1327 977 AgeI A'CCGG_T Cuts at: 5396 5396 Size: 6565 AhdI GACnn_n'nnGTC Cuts at: 5294 5294 Size: 6565 AleI CACnn'nnGTG Cuts at: 1166 3688 1166 Size: 2522 4043 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2254 1184 Size: 1070 5495 AlwNI CAG_nnn'CTG Cuts at: 2364 3418 4308 5880 2364 Size: 1054 890 1572 3049 Fragments arranged by size: 3049 1572 1054 890 ApaLI G'TGCA_C Cuts at: 4622 5778 4622 Size: 1156 5409 ApoI r'AATT_y Cuts at: 1493 3704 3874 4100 4252 6361 1493 Size: 2211 170 226 152 2109 1697 Fragments arranged by size: 2211 2109 1697 226 170 152 AseI AT'TA_AT Cuts at: 160 1032 5119 160 Size: 872 4087 1606 Fragments arranged by size: 4087 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6565 AvaI C'yCGr_G Cuts at: 1870 1943 3890 1870 Size: 73 1947 4545 Fragments arranged by size: 4545 1947 73 BamHI G'GATC_C Cuts at: 3895 4262 3895 Size: 367 6198 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2238 3886 5341 618 Size: 325 217 627 451 1648 1455 1842 Fragments arranged by size: 1842 1648 1455 627 451 325 217 BanII G_rGCy'C Cuts at: 1391 2298 3884 1391 Size: 907 1586 4072 Fragments arranged by size: 4072 1586 907 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2373 2396 3210 3668 928 Size: 971 474 23 814 458 3825 Fragments arranged by size: 3825 971 814 474 458 23 BbvCI CC'TCA_GC Cuts at: 3390 3390 Size: 6565 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4756 4790 1903 Size: 34 2819 34 3678 Fragments arranged by size: 3678 2819 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4457 5667 4457 Size: 1210 5355 BclI T'GATC_A Cuts at: 1548 1548 Size: 6565 BfrBI ATG'CAT Cuts at: 3220 3220 Size: 6565 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5176 136 Size: 107 122 71 631 605 3504 1525 Fragments arranged by size: 3504 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6560 6560 Size: 6565 BlpI GC'TnA_GC Cuts at: 3982 3982 Size: 6565 Bme1580I G_kGCm'C Cuts at: 1790 4626 5782 1790 Size: 2836 1156 2573 Fragments arranged by size: 2836 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6565 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2374 4296 5254 Size: 416 53 83 399 24 957 1922 958 Cuts at: 5254 442 Size: 1753 Fragments arranged by size: 1922 1753 958 957 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6565 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2341 3756 5225 1239 Size: 1102 1415 1469 2579 Fragments arranged by size: 2579 1469 1415 1102 Bpu10I CC'TnA_GC Cuts at: 2573 3390 2573 Size: 817 5748 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3011 3745 4690 5555 5853 3011 Size: 734 945 865 298 3723 Fragments arranged by size: 3723 945 865 734 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5228 882 Size: 617 3729 2219 Fragments arranged by size: 3729 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4356 493 Size: 711 3152 2702 Fragments arranged by size: 3152 2702 711 BsaBI GATnn'nnATC Cuts at: 2886 6559 2886 Size: 3673 2892 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4372 4754 5389 275 Size: 53 83 186 936 2839 382 635 1451 Fragments arranged by size: 2839 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3199 4998 5396 5670 5817 6228 3199 Size: 1799 398 274 147 411 3536 Fragments arranged by size: 3536 1799 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6340 Fragments arranged by size: 6340 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6565 BseYI C'CCAG_C Cuts at: 5768 6264 5768 Size: 496 6069 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5992 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4015 4776 5804 1540 Size: 275 186 2014 761 1028 2301 Fragments arranged by size: 2301 2014 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2071 2262 3054 4626 4711 5782 2071 Size: 191 792 1572 85 1071 2854 Fragments arranged by size: 2854 1572 1071 792 191 85 BsmI GAATG_Cn' Cuts at: 4076 4169 4076 Size: 93 6472 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2071 2262 2298 3054 3884 4626 4711 Size: 399 281 191 36 756 830 742 85 Cuts at: 4711 5782 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 830 756 742 399 281 191 85 36 BspEI T'CCGG_A Cuts at: 6228 6228 Size: 6565 BspHI T'CATG_A Cuts at: 4454 6184 4454 Size: 1730 4835 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3016 3920 844 Size: 2172 904 3489 Fragments arranged by size: 3489 2172 904 BsrBI CCG'CTC Cuts at: 1394 1823 2017 4452 1394 Size: 429 194 2435 3507 Fragments arranged by size: 3507 2435 429 194 BsrDI GCAATG_nn' Cuts at: 65 1248 3192 3565 5060 5234 65 Size: 1183 1944 373 1495 174 1396 Fragments arranged by size: 1944 1495 1396 1183 373 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5209 5396 1700 Size: 110 3399 187 2869 Fragments arranged by size: 3399 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2812 3579 96 Size: 2716 767 3082 Fragments arranged by size: 3082 2716 767 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6565 BssSI C'ACGA_G Cuts at: 2103 4625 5637 2103 Size: 2522 1012 3031 Fragments arranged by size: 3031 2522 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6565 BstBI TT'CG_AA Cuts at: 5369 5369 Size: 6565 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6565 BstYI r'GATC_y Cuts at: 1972 2350 2785 3895 4262 4647 4664 6116 6202 Size: 378 435 1110 367 385 17 1452 86 Cuts at: 6202 6214 6560 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1110 435 385 378 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3689 513 Size: 552 2624 3389 Fragments arranged by size: 3389 2624 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3077 487 Size: 683 56 1851 3975 Fragments arranged by size: 3975 1851 683 56 BtsI GCAGTG_nn' Cuts at: 2922 4081 4875 4895 2922 Size: 1159 794 20 4592 Fragments arranged by size: 4592 1159 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6565 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2971 3006 529 Size: 35 2407 35 4088 Fragments arranged by size: 4088 2407 35 35 DraI TTT'AAA Cuts at: 2746 3869 2746 Size: 1123 5442 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3847 5572 809 Size: 507 2531 1725 1802 Fragments arranged by size: 2531 1802 1725 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4012 4901 6530 Size: 54 1425 50 275 18 2182 889 1629 Cuts at: 6530 8 Size: 43 Fragments arranged by size: 2182 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4012 1537 Size: 275 2200 4090 Fragments arranged by size: 4090 2200 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2286 4495 1353 Size: 933 2209 3423 Fragments arranged by size: 3423 2209 933 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5152 5526 5672 387 Size: 1064 138 3563 374 146 1280 Fragments arranged by size: 3563 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2341 3160 3615 3756 4628 5225 6012 Size: 393 709 819 455 141 872 597 787 Cuts at: 6012 6280 1239 Size: 268 1524 Fragments arranged by size: 1524 872 819 787 709 597 455 393 268 141 EcoO109I rG'GnC_Cy Cuts at: 2269 2269 Size: 6565 EcoRI G'AATT_C Cuts at: 3874 4252 3874 Size: 378 6187 EcoRV GAT'ATC Cuts at: 2969 2969 Size: 6565 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2573 2573 Size: 6565 FspI TGC'GCA Cuts at: 5071 5071 Size: 6565 HaeII r_GCGC'y Cuts at: 1678 5712 1678 Size: 4034 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5206 5238 5280 5312 1373 Size: 32 135 32 3634 32 42 32 2626 Fragments arranged by size: 3634 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3328 3909 4155 669 Size: 988 281 1390 581 246 3079 Fragments arranged by size: 3079 1390 988 581 281 246 HindIII A'AGCT_T Cuts at: 748 2469 2477 2759 3597 748 Size: 1721 8 282 838 3716 Fragments arranged by size: 3716 1721 838 282 8 HpaI GTT'AAC Cuts at: 4155 4155 Size: 6565 KpnI G_GTAC'C Cuts at: 3890 3890 Size: 6565 MfeI C'AATT_G Cuts at: 3306 4164 3306 Size: 858 5707 MluI A'CGCG_T Cuts at: 6441 6441 Size: 6565 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5679 5863 1305 Size: 4374 184 2007 Fragments arranged by size: 4374 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4743 Fragments arranged by size: 4743 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2949 3575 3688 4523 4882 5041 518 Size: 648 1783 626 113 835 359 159 2042 Fragments arranged by size: 2042 1783 835 648 626 359 159 113 MspA1I CmG'CkG Cuts at: 1606 3091 3981 4658 5806 6051 1606 Size: 1485 890 677 1148 245 2120 Fragments arranged by size: 2120 1485 1148 890 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6013 NdeI CA'TA_TG Cuts at: 387 387 Size: 6565 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6565 NotI GC'GGCC_GC Cuts at: 4012 4012 Size: 6565 NsiI A_TGCA'T Cuts at: 3222 3222 Size: 6565 NspI r_CATG'y Cuts at: 1132 1229 2220 2530 2960 3365 3923 5468 1132 Size: 97 991 310 430 405 558 1545 2229 Fragments arranged by size: 2229 1545 991 558 430 405 310 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6565 PciI A'CATG_T Cuts at: 5464 5464 Size: 6565 PflMI CCAn_nnn'nTGG Cuts at: 1108 2364 3064 1108 Size: 1256 700 4609 Fragments arranged by size: 4609 1256 700 PmeI GTTT'AAAC Cuts at: 3869 3869 Size: 6565 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4634 4666 6147 6179 4634 Size: 32 1481 32 5020 Fragments arranged by size: 5020 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6565 PsiI TTA'TAA Cuts at: 2733 4135 2733 Size: 1402 5163 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2434 2466 2434 Size: 32 6533 PstI C_TGCA'G Cuts at: 830 3371 3730 3917 830 Size: 2541 359 187 3478 Fragments arranged by size: 3478 2541 359 187 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6565 PvuII CAG'CTG Cuts at: 3091 3091 Size: 6565 SalI G'TCGA_C Cuts at: 1936 3907 1936 Size: 1971 4594 SapI GCTCTTCn'nnn_ Cuts at: 2286 2286 Size: 6565 SbfI CC_TGCA'GG Cuts at: 3917 3917 Size: 6565 ScaI AGT'ACT Cuts at: 4813 6525 4813 Size: 1712 4853 SfcI C'TryA_G Cuts at: 826 1045 2455 2673 2769 3367 3726 3913 5048 Size: 219 1410 218 96 598 359 187 1135 Cuts at: 5048 5729 5920 826 Size: 681 191 1471 Fragments arranged by size: 1471 1410 1135 681 598 359 219 218 191 187 96 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6565 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6565 SmaI CCC'GGG Cuts at: 1872 3892 1872 Size: 2020 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2990 3760 4669 5570 5832 820 Size: 197 926 1047 770 909 901 262 1553 Fragments arranged by size: 1553 1047 926 909 901 770 262 197 SnaBI TAC'GTA Cuts at: 493 4356 493 Size: 3863 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6565 SphI G_CATG'C Cuts at: 1132 3923 1132 Size: 2791 3774 SspI AAT'ATT Cuts at: 5 52 4489 5 Size: 47 4437 2081 Fragments arranged by size: 4437 2081 47 StuI AGG'CCT Cuts at: 1517 3395 1517 Size: 1878 4687 StyI C'CwwG_G Cuts at: 513 1065 3396 3520 3789 4004 513 Size: 552 2331 124 269 215 3074 Fragments arranged by size: 3074 2331 552 269 215 124 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2175 2930 4592 4609 4762 4947 5392 Size: 1255 595 755 1662 17 153 185 445 Cuts at: 5392 325 Size: 1498 Fragments arranged by size: 1662 1498 1255 755 595 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2812 3579 4811 Size: 274 80 33 51 1443 835 767 1232 Cuts at: 4811 6523 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1232 835 767 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6525 6557 6525 Size: 32 6533 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6565 XbaI T'CTAG_A Cuts at: 2431 3901 2431 Size: 1470 5095 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3518 3787 3518 Size: 269 6296 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6565 XmaI C'CCGG_G Cuts at: 1870 3890 1870 Size: 2020 4545 XmnI GAAnn'nnTTC Cuts at: 4694 4694 Size: 6565 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4373 276 Size: 53 83 186 936 2839 2468 Fragments arranged by size: 2839 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI