(Circular) (Six-base) MAPSORT of: pFN21AE0331.seq Check: 547 from: 1 to: 6667 pFN21AE0331 6667 bp With 182 enzymes: SgfI * April 15, 2010 09:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4477 278 Size: 53 83 186 936 2941 2468 Fragments arranged by size: 2941 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3672 4010 1937 Size: 1735 338 4594 Fragments arranged by size: 4594 1735 338 Acc65I G'GTAC_C Cuts at: 3988 3988 Size: 6667 AclI AA'CG_TT Cuts at: 1563 4794 5167 1563 Size: 3231 373 3063 Fragments arranged by size: 3231 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2577 2640 2727 3018 4730 6114 6382 1632 Size: 945 63 87 291 1712 1384 268 1917 Fragments arranged by size: 1917 1712 1384 945 291 268 87 63 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6470 AflIII A'CryG_T Cuts at: 1203 5566 6543 1203 Size: 4363 977 1327 Fragments arranged by size: 4363 1327 977 AgeI A'CCGG_T Cuts at: 5498 5498 Size: 6667 AhdI GACnn_n'nnGTC Cuts at: 2058 5396 2058 Size: 3338 3329 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6667 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6667 AlwNI CAG_nnn'CTG Cuts at: 3460 3814 4410 5982 3460 Size: 354 596 1572 4145 Fragments arranged by size: 4145 1572 596 354 ApaLI G'TGCA_C Cuts at: 4724 5880 4724 Size: 1156 5511 ApoI r'AATT_y Cuts at: 1493 2012 2322 3500 3603 3655 3853 3976 4202 Size: 519 310 1178 103 52 198 123 226 Cuts at: 4202 4354 6463 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1178 519 310 226 198 152 123 103 52 AseI AT'TA_AT Cuts at: 160 1032 2990 5221 160 Size: 872 1958 2231 1606 Fragments arranged by size: 2231 1958 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6667 AvaI C'yCGr_G Cuts at: 1870 1943 3992 1870 Size: 73 2049 4545 Fragments arranged by size: 4545 2049 73 BamHI G'GATC_C Cuts at: 3997 4364 3997 Size: 367 6300 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3988 5443 618 Size: 325 217 627 2201 1455 1842 Fragments arranged by size: 2201 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3986 1391 Size: 2595 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2351 2535 3756 928 Size: 971 452 184 1221 3839 Fragments arranged by size: 3839 1221 971 452 184 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3668 3702 4858 4892 1903 Size: 34 1731 34 1156 34 3678 Fragments arranged by size: 3678 1731 1156 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2968 4559 5769 2968 Size: 1591 1210 3866 Fragments arranged by size: 3866 1591 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6667 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5278 136 Size: 107 122 71 631 605 3606 1525 Fragments arranged by size: 3606 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3707 6662 3707 Size: 2955 3712 BlpI GC'TnA_GC Cuts at: 4084 4084 Size: 6667 Bme1580I G_kGCm'C Cuts at: 1790 4728 5884 1790 Size: 2938 1156 2573 Fragments arranged by size: 2938 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6667 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2599 4398 5356 Size: 416 53 83 399 24 1182 1799 958 Cuts at: 5356 442 Size: 1753 Fragments arranged by size: 1799 1753 1182 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6667 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2084 2688 3516 5327 1239 Size: 845 604 828 1811 2579 Fragments arranged by size: 2579 1811 845 828 604 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3107 3136 4792 5657 5955 3107 Size: 29 1656 865 298 3819 Fragments arranged by size: 3819 1656 865 298 29 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3583 5330 882 Size: 617 2084 1747 2219 Fragments arranged by size: 2219 2084 1747 617 BsaAI yAC'GTr Cuts at: 493 1204 4458 493 Size: 711 3254 2702 Fragments arranged by size: 3254 2702 711 BsaBI GATnn'nnATC Cuts at: 3516 6661 3516 Size: 3145 3522 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4474 4856 5491 275 Size: 53 83 186 936 2941 382 635 1451 Fragments arranged by size: 2941 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5100 5498 5772 5919 6330 5100 Size: 398 274 147 411 5437 Fragments arranged by size: 5437 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6442 Fragments arranged by size: 6442 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2709 1186 Size: 1523 5144 BseYI C'CCAG_C Cuts at: 5870 6366 5870 Size: 496 6171 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6094 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4117 4878 5906 1540 Size: 275 186 2116 761 1028 2301 Fragments arranged by size: 2301 2116 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2904 4728 4813 5884 2904 Size: 1824 85 1071 3687 Fragments arranged by size: 3687 1824 1071 85 BsmI GAATG_Cn' Cuts at: 4178 4271 4178 Size: 93 6574 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2904 3986 4728 4813 5884 1391 Size: 399 1114 1082 742 85 1071 2174 Fragments arranged by size: 2174 1114 1082 1071 742 399 85 BspEI T'CCGG_A Cuts at: 6330 6330 Size: 6667 BspHI T'CATG_A Cuts at: 3849 4556 6286 3849 Size: 707 1730 4230 Fragments arranged by size: 4230 1730 707 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4022 844 Size: 3178 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4554 1394 Size: 429 2731 3507 Fragments arranged by size: 3507 2731 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5162 5336 65 Size: 1183 3914 174 1396 Fragments arranged by size: 3914 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5311 5498 1700 Size: 110 3501 187 2869 Fragments arranged by size: 3501 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2740 3068 96 Size: 2644 328 3695 Fragments arranged by size: 3695 2644 328 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6667 BssSI C'ACGA_G Cuts at: 4727 5739 4727 Size: 1012 5655 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6667 BstBI TT'CG_AA Cuts at: 3659 5471 3659 Size: 1812 4855 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6667 BstYI r'GATC_y Cuts at: 1972 3707 3997 4364 4749 4766 6218 6304 6316 Size: 1735 290 367 385 17 1452 86 12 Cuts at: 6316 6662 1972 Size: 346 1977 Fragments arranged by size: 1977 1735 1452 385 367 346 290 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5176 Fragments arranged by size: 5176 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5928 Fragments arranged by size: 5928 683 56 BtsI GCAGTG_nn' Cuts at: 2781 4183 4977 4997 2781 Size: 1402 794 20 4451 Fragments arranged by size: 4451 1402 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6667 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6632 DraI TTT'AAA Cuts at: 3907 3971 3907 Size: 64 6603 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5674 809 Size: 507 4358 1802 Fragments arranged by size: 4358 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4114 5003 6632 Size: 54 1425 50 275 18 2284 889 1629 Cuts at: 6632 8 Size: 43 Fragments arranged by size: 2284 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4114 1537 Size: 275 2302 4090 Fragments arranged by size: 4090 2302 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2190 2295 2703 4597 1353 Size: 837 105 408 1894 3423 Fragments arranged by size: 3423 1894 837 408 105 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5254 5628 5774 387 Size: 1064 138 3665 374 146 1280 Fragments arranged by size: 3665 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2084 2577 2640 2688 2727 3018 3516 Size: 393 452 493 63 48 39 291 498 Cuts at: 3516 4730 5327 6114 6382 1239 Size: 1214 597 787 268 1524 Fragments arranged by size: 1524 1214 787 597 498 493 452 393 291 268 63 48 39 EcoO109I rG'GnC_Cy Cuts at: 2159 3683 2159 Size: 1524 5143 EcoRI G'AATT_C Cuts at: 3603 3853 3976 4354 3603 Size: 250 123 378 5916 Fragments arranged by size: 5916 378 250 123 EcoRV GAT'ATC Cuts at: 2202 2563 2202 Size: 361 6306 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3918 3918 Size: 6667 FspI TGC'GCA Cuts at: 5173 5173 Size: 6667 HaeII r_GCGC'y Cuts at: 1678 5814 1678 Size: 4136 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2044 2076 2274 2306 2441 Size: 32 135 32 472 32 198 32 135 Cuts at: 2441 2473 3448 3480 3563 3595 5308 5340 5382 Size: 32 975 32 83 32 1713 32 42 Cuts at: 5382 5414 1373 Size: 32 2626 Fragments arranged by size: 2626 1713 975 472 198 135 135 83 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2334 4011 4257 669 Size: 988 281 396 1677 246 3079 Fragments arranged by size: 3079 1677 988 396 281 246 HindIII A'AGCT_T Cuts at: 748 2118 748 Size: 1370 5297 HpaI GTT'AAC Cuts at: 4257 4257 Size: 6667 KpnI G_GTAC'C Cuts at: 3992 3992 Size: 6667 MfeI C'AATT_G Cuts at: 3870 4266 3870 Size: 396 6271 MluI A'CGCG_T Cuts at: 6543 6543 Size: 6667 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5781 5965 1305 Size: 4476 184 2007 Fragments arranged by size: 4476 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4845 Fragments arranged by size: 4845 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4625 4984 5143 518 Size: 648 3459 359 159 2042 Fragments arranged by size: 3459 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2660 4083 4760 5908 6153 1606 Size: 1054 1423 677 1148 245 2120 Fragments arranged by size: 2120 1423 1148 1054 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5176 Fragments arranged by size: 5176 939 552 NdeI CA'TA_TG Cuts at: 387 3169 387 Size: 2782 3885 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6667 NotI GC'GGCC_GC Cuts at: 4114 4114 Size: 6667 NspI r_CATG'y Cuts at: 1132 1229 2645 4025 5570 1132 Size: 97 1416 1380 1545 2229 Fragments arranged by size: 2229 1545 1416 1380 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6667 PciI A'CATG_T Cuts at: 5566 5566 Size: 6667 PflMI CCAn_nnn'nTGG Cuts at: 1108 2928 1108 Size: 1820 4847 PmeI GTTT'AAAC Cuts at: 3971 3971 Size: 6667 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4736 4768 6249 6281 4736 Size: 32 1481 32 5122 Fragments arranged by size: 5122 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2159 2159 Size: 6667 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6667 PsiI TTA'TAA Cuts at: 4237 4237 Size: 6667 PstI C_TGCA'G Cuts at: 830 4019 830 Size: 3189 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6667 PvuII CAG'CTG Cuts at: 2660 2660 Size: 6667 SalI G'TCGA_C Cuts at: 1936 4009 1936 Size: 2073 4594 SbfI CC_TGCA'GG Cuts at: 4019 4019 Size: 6667 ScaI AGT'ACT Cuts at: 2518 4915 6627 2518 Size: 2397 1712 2558 Fragments arranged by size: 2558 2397 1712 SfcI C'TryA_G Cuts at: 826 1045 4015 5150 5831 6022 826 Size: 219 2970 1135 681 191 1471 Fragments arranged by size: 2970 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6667 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6667 SmaI CCC'GGG Cuts at: 1872 3994 1872 Size: 2122 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3115 3122 4771 5672 5934 820 Size: 197 926 1172 7 1649 901 262 1553 Fragments arranged by size: 1649 1553 1172 926 901 262 197 7 SnaBI TAC'GTA Cuts at: 493 4458 493 Size: 3965 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6667 SphI G_CATG'C Cuts at: 1132 4025 1132 Size: 2893 3774 SspI AAT'ATT Cuts at: 5 52 3078 4591 5 Size: 47 3026 1513 2081 Fragments arranged by size: 3026 2081 1513 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6667 StyI C'CwwG_G Cuts at: 513 1065 2004 2867 3174 3404 4106 513 Size: 552 939 863 307 230 702 3074 Fragments arranged by size: 3074 939 863 702 552 307 230 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4694 4711 4864 5049 5494 325 Size: 1255 3114 17 153 185 445 1498 Fragments arranged by size: 3114 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2069 2516 2740 Size: 274 80 33 51 1443 92 447 224 Cuts at: 2740 3068 4913 6625 96 Size: 328 1845 1712 138 Fragments arranged by size: 1845 1712 1443 447 328 274 224 138 92 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2225 2257 6627 6659 2225 Size: 32 4370 32 2233 Fragments arranged by size: 4370 2233 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2875 1316 Size: 1559 5108 XbaI T'CTAG_A Cuts at: 4003 4003 Size: 6667 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3457 3457 Size: 6667 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6667 XmaI C'CCGG_G Cuts at: 1870 3992 1870 Size: 2122 4545 XmnI GAAnn'nnTTC Cuts at: 3380 4796 3380 Size: 1416 5251 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4475 276 Size: 53 83 186 936 2941 2468 Fragments arranged by size: 2941 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI