Restriction map for
pFN21AE0329
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# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Alw44I AlwI
AocI ApaI ApaLI AscI Asp718I
AspEI AspHI AspI AviII AvrII
BalI BamHI BanII BbrPI BbvCI
BcgI BciVI BclI BcnI BfrI
BglII BlnI BlpI BmyI Bpu1102I
BsaAI BsaBI BsaWI BseAI BsiHKAI
BsiWI BsiYI BslI BsmFI Bsp1286I
BspEI BspHI BspLU11I BsrDI BsrFI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
CelII Cfr10I CpoI DraIII DrdI
DsaI Eam1105I Eco81I EcoNI EcoO65I
EcoRV FauI FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
KspI MamI MfeI MflI MluI
MluNI MroI MscI MunI NaeI
NciI NcoI NdeI NgoMIV NotI
NspI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI Tsp45I Tth111I
Van91I XcmI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AcyI 16 GGCGCC G[GA]^CG[CT]C
AlwNI 494 CAGATACTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1162 AGCGCT AGC^GCT
AseI 1281 ATTAAT AT^TAAT
AsnI 1281 ATTAAT AT^TAAT
Asp700I 284 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 791 CTCGAG C^[CT]CG[GA]G
BanI 16 GGCGCC G^G[CT][GA]CC
BbeI 16 GGCGCC GGCGC^C
BglI 19 GCCATTCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 16 GGCGCC G[GA]^CG[CT]C
BsaI 567 GAGACC GGTCTC(1/5)
BsiEI 26 CGGCCG CG[GA][CT]^CG
BsmBI 1424 GAGACG CGTCTC(1/5)
BsmI 746 GAATGC GAATGC(1/-1)
BsoBI 791 CTCGAG C^[CT]CG[GA]G
Bsp1407I 1209 TGTACA T^GTACA
BspDI 172 ATCGAT AT^CGAT
BsrBI 51 GAGCGG CCGCTC(-3/-3)
BsrGI 1209 TGTACA T^GTACA
BstAPI 520 GCAGGCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI 172 ATCGAT AT^CGAT
DpnII 562 GATC ^GATC
DraI 1432 TTTAAA TTT^AAA
DraII 578 AGGACCT [GA]G^G[ACGT]CC[CT]
EagI 26 CGGCCG C^GGCCG
EclXI 26 CGGCCG C^GGCCG
Eco47III 1162 AGCGCT AGC^GCT
Eco52I 26 CGGCCG C^GGCCG
EcoO109I 578 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 266 CCTTGG C^C[AT][AT]GG
EcoT22I 334 ATGCAT ATGCA^T
HapII 25 CCGG C^CGG
HgaI 406 GCGTC GACGC(5/10)
Hin1I 16 GGCGCC G[GA]^CG[CT]C
HpaII 25 CCGG C^CGG
KasI 16 GGCGCC G^GCGCC
MboI 562 GATC ^GATC
MslI 588 CATCCGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1221 CAGCGG C[AC]G^C[GT]G
MspI 25 CCGG C^CGG
NarI 16 GGCGCC GG^CGCC
NdeII 562 GATC ^GATC
NheI 894 GCTAGC G^CTAGC
NruI 35 TCGCGA TCG^CGA
NsiI 334 ATGCAT ATGCA^T
PaeR7I 791 CTCGAG C^TCGAG
PleI 1464 GACTC GAGTC(4/5)
PpuMI 578 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 1281 ATTAAT AT^TAAT
Sau3AI 562 GATC ^GATC
SfoI 16 GGCGCC GGC^GCC
SspBI 1209 TGTACA T^GTACA
StyI 266 CCTTGG C^C[AT][AT]GG
TfiI 928 GAATC G^A[AT]TC
XbaI 1023 TCTAGA T^CTAGA
XhoI 791 CTCGAG C^TCGAG
XmnI 284 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 96 GTCTAC GT^[AC][GT]AC
393 GTCTAC
AccII 36 CGCG CG^CG
57 CGCG
AfaI 1174 GTAC GT^AC
1210 GTAC
BpmI 751 CTGGAG CTGGAG(16/14)
1275 CTGGAG
BspMI 570 ACCTGC ACCTGC(4/8)
79 GCAGGT
BssKI 12 CCAGG ^CC[ACGT]GG
1100 CCAGG
BstNI 12 CCAGG CC^[AT]GG
1100 CCAGG
BstUI 36 CGCG CG^CG
57 CGCG
EaeI 26 CGGCCG [CT]^GGCC[GA]
41 CGGCCA
EcoRII 12 CCAGG ^CC[AT]GG
1100 CCAGG
HaeII 16 GGCGCC [GA]GCGC^[CT]
1162 AGCGCT
HinfI 928 GAATC G^A[ACGT]TC
1464 GACTC
HphI 82 GGTGA GGTGA(8/7)
1477 GGTGA
MaeII 798 ACGT A^CGT
1419 ACGT
MvaI 12 CCAGG CC^[AT]GG
1100 CCAGG
MvnI 36 CGCG CG^CG
57 CGCG
NlaIII 526 CATG CATG^
1092 CATG
PstI 293 CTGCAG CTGCA^G
572 CTGCAG
RsaI 1174 GTAC GT^AC
1210 GTAC
ScrFI 12 CCAGG CC^[ACGT]GG
1100 CCAGG
SmlI 791 CTCGAG C^T[CT][GA]AG
1466 CTCAAG
TaqI 173 TCGA T^CGA
792 TCGA
TthHB8I 173 TCGA T^CGA
792 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AciI AcsI AluI ApoI AvaII
BbsI BbvI BfaI BpuAI BsaJI
BseRI BsgI BsmAI BsrI BstF5I
Cac8I CfoI Cfr13I DdeI EarI
EcoRI Fnu4HI FokI HaeIII HhaI
HinP1I ItaI Ksp632I MaeI MaeIII
MboII MnlI MseI MwoI NlaIV
Sau96I SfaNI SfcI Tru9I TseI
Tsp509I TspRI