(Circular) (Six-base) MAPSORT of: pFN21AE0329.seq Check: 9299 from: 1 to: 6229 pFN21AE0329 6229 bp With 182 enzymes: SgfI * April 15, 2010 09:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2075 2075 Size: 6229 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4039 278 Size: 53 83 186 936 2503 2468 Fragments arranged by size: 2503 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2102 2399 3572 1937 Size: 165 297 1173 4594 Fragments arranged by size: 4594 1173 297 165 Acc65I G'GTAC_C Cuts at: 3550 3550 Size: 6229 AclI AA'CG_TT Cuts at: 1563 4356 4729 1563 Size: 2793 373 3063 Fragments arranged by size: 3063 2793 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3315 4292 5676 5944 1632 Size: 1683 977 1384 268 1917 Fragments arranged by size: 1917 1683 1384 977 268 AfeI AGC'GCT Cuts at: 3169 3169 Size: 6229 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6032 AflIII A'CryG_T Cuts at: 1203 5128 6105 1203 Size: 3925 977 1327 Fragments arranged by size: 3925 1327 977 AgeI A'CCGG_T Cuts at: 5060 5060 Size: 6229 AhdI GACnn_n'nnGTC Cuts at: 4958 4958 Size: 6229 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6229 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3066 1184 Size: 1882 4347 AlwNI CAG_nnn'CTG Cuts at: 2504 3972 5544 2504 Size: 1468 1572 3189 Fragments arranged by size: 3189 1572 1468 ApaLI G'TGCA_C Cuts at: 4286 5442 4286 Size: 1156 5073 ApoI r'AATT_y Cuts at: 1493 2265 2319 2967 3113 3453 3511 3538 3764 Size: 772 54 648 146 340 58 27 226 Cuts at: 3764 3916 6025 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 772 648 340 226 152 146 58 54 27 AseI AT'TA_AT Cuts at: 160 1032 3287 4783 160 Size: 872 2255 1496 1606 Fragments arranged by size: 2255 1606 1496 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6229 AvaI C'yCGr_G Cuts at: 1870 1943 2796 3554 1870 Size: 73 853 758 4545 Fragments arranged by size: 4545 853 758 73 BamHI G'GATC_C Cuts at: 3559 3926 3559 Size: 367 5862 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2021 3550 5005 618 Size: 325 217 627 234 1529 1455 1842 Fragments arranged by size: 1842 1529 1455 627 325 234 217 BanII G_rGCy'C Cuts at: 1391 3548 1391 Size: 2157 4072 BbeI G_GCGC'C Cuts at: 2025 2025 Size: 6229 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2059 2972 3202 928 Size: 971 160 913 230 3955 Fragments arranged by size: 3955 971 913 230 160 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4420 4454 1903 Size: 34 50 34 2399 34 3678 Fragments arranged by size: 3678 2399 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4121 5331 4121 Size: 1210 5019 BclI T'GATC_A Cuts at: 1548 1548 Size: 6229 BfrBI ATG'CAT Cuts at: 2341 2341 Size: 6229 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2030 4840 136 Size: 107 122 71 631 605 358 2810 1525 Fragments arranged by size: 2810 1525 631 605 358 122 107 71 BglII A'GATC_T Cuts at: 6224 6224 Size: 6229 BlpI GC'TnA_GC Cuts at: 3646 3646 Size: 6229 Bme1580I G_kGCm'C Cuts at: 1790 4290 5446 1790 Size: 2500 1156 2573 Fragments arranged by size: 2573 2500 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6229 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3960 4918 442 Size: 416 53 83 399 24 2543 958 1753 Fragments arranged by size: 2543 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2903 1056 Size: 1847 4382 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2862 2862 Size: 6229 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2777 3301 4889 1239 Size: 1538 524 1588 2579 Fragments arranged by size: 2579 1588 1538 524 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3456 4354 5219 5517 3456 Size: 898 865 298 4168 Fragments arranged by size: 4168 898 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2566 4892 882 Size: 617 1067 2326 2219 Fragments arranged by size: 2326 2219 1067 617 BsaAI yAC'GTr Cuts at: 493 1204 4020 493 Size: 711 2816 2702 Fragments arranged by size: 2816 2702 711 BsaBI GATnn'nnATC Cuts at: 6223 6223 Size: 6229 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2022 4036 4418 5053 Size: 53 83 186 936 489 2014 382 635 Cuts at: 5053 275 Size: 1451 Fragments arranged by size: 2014 1451 936 635 489 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4662 5060 5334 5481 5892 4662 Size: 398 274 147 411 4999 Fragments arranged by size: 4999 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6004 Fragments arranged by size: 6004 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2529 2701 3015 1186 Size: 1343 172 314 4400 Fragments arranged by size: 4400 1343 314 172 BseYI C'CCAG_C Cuts at: 5432 5928 5432 Size: 496 5733 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2544 2853 3036 1163 Size: 573 808 309 183 4356 Fragments arranged by size: 4356 808 573 309 183 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2034 3679 4440 5468 1540 Size: 275 186 33 1645 761 1028 2301 Fragments arranged by size: 2301 1645 1028 761 275 186 33 BsiHKAI G_wGCw'C Cuts at: 4290 4375 5446 4290 Size: 85 1071 5073 Fragments arranged by size: 5073 1071 85 BsmI GAATG_Cn' Cuts at: 2757 3740 3833 2757 Size: 983 93 5153 Fragments arranged by size: 5153 983 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3423 3423 Size: 6229 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3548 4290 4375 5446 1391 Size: 399 1758 742 85 1071 2174 Fragments arranged by size: 2174 1758 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5892 5892 Size: 6229 BspHI T'CATG_A Cuts at: 4118 5848 4118 Size: 1730 4499 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2075 2584 3584 844 Size: 1231 509 1000 3489 Fragments arranged by size: 3489 1231 1000 509 BsrBI CCG'CTC Cuts at: 1394 1823 2058 4116 1394 Size: 429 235 2058 3507 Fragments arranged by size: 3507 2058 429 235 BsrDI GCAATG_nn' Cuts at: 65 1248 4724 4898 65 Size: 1183 3476 174 1396 Fragments arranged by size: 3476 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4873 5060 1700 Size: 110 3063 187 2869 Fragments arranged by size: 3063 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3214 96 Size: 3118 3111 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6229 BssSI C'ACGA_G Cuts at: 4289 5301 4289 Size: 1012 5217 BstAPI GCAn_nnn'nTGC Cuts at: 794 2531 794 Size: 1737 4492 BstBI TT'CG_AA Cuts at: 5033 5033 Size: 6229 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6229 BstYI r'GATC_y Cuts at: 1972 3559 3926 4311 4328 5780 5866 5878 6224 Size: 1587 367 385 17 1452 86 12 346 Cuts at: 6224 1972 Size: 1977 Fragments arranged by size: 1977 1587 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5677 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2023 487 Size: 683 56 797 4693 Fragments arranged by size: 4693 797 683 56 BtsI GCAGTG_nn' Cuts at: 3745 4539 4559 3745 Size: 794 20 5415 Fragments arranged by size: 5415 794 20 ClaI AT'CG_AT Cuts at: 1554 2178 1554 Size: 624 5605 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6194 DraI TTT'AAA Cuts at: 3439 3533 3439 Size: 94 6135 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5236 809 Size: 507 3920 1802 Fragments arranged by size: 3920 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2031 2046 3676 Size: 54 1425 50 275 18 201 15 1630 Cuts at: 3676 4565 6194 8 Size: 889 1629 43 Fragments arranged by size: 1630 1629 1425 889 275 201 54 50 43 18 15 EagI C'GGCC_G Cuts at: 1537 1812 2031 3676 1537 Size: 275 219 1645 4090 Fragments arranged by size: 4090 1645 275 219 EarI CTCTTCn'nnn_ Cuts at: 1353 2631 3027 3226 4159 1353 Size: 1278 396 199 933 3423 Fragments arranged by size: 3423 1278 933 396 199 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3303 4816 5190 5336 387 Size: 1064 138 1714 1513 374 146 1280 Fragments arranged by size: 1714 1513 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2777 3301 3315 4292 4889 5676 5944 Size: 393 1145 524 14 977 597 787 268 Cuts at: 5944 1239 Size: 1524 Fragments arranged by size: 1524 1145 977 787 597 524 393 268 14 EcoO109I rG'GnC_Cy Cuts at: 2584 2584 Size: 6229 EcoRI G'AATT_C Cuts at: 2265 2319 2967 3538 3916 2265 Size: 54 648 571 378 4578 Fragments arranged by size: 4578 648 571 378 54 FspI TGC'GCA Cuts at: 4735 4735 Size: 6229 HaeII r_GCGC'y Cuts at: 1678 2025 3171 5376 1678 Size: 347 1146 2205 2531 Fragments arranged by size: 2531 2205 1146 347 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2084 2116 2480 2512 4870 Size: 32 135 32 512 32 364 32 2358 Cuts at: 4870 4902 4944 4976 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2358 512 364 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3573 3819 669 Size: 988 281 1635 246 3079 Fragments arranged by size: 3079 1635 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6229 HpaI GTT'AAC Cuts at: 3819 3819 Size: 6229 KasI G'GCGC_C Cuts at: 2021 2021 Size: 6229 KpnI G_GTAC'C Cuts at: 3554 3554 Size: 6229 MfeI C'AATT_G Cuts at: 3828 3828 Size: 6229 MluI A'CGCG_T Cuts at: 6105 6105 Size: 6229 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2356 2691 5343 5527 1305 Size: 1051 335 2652 184 2007 Fragments arranged by size: 2652 2007 1051 335 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4407 Fragments arranged by size: 4407 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2597 4187 4546 4705 518 Size: 648 1431 1590 359 159 2042 Fragments arranged by size: 2042 1590 1431 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3228 3645 4322 5470 5715 1606 Size: 1622 417 677 1148 245 2120 Fragments arranged by size: 2120 1622 1148 677 417 245 NarI GG'CG_CC Cuts at: 2022 2022 Size: 6229 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5677 NdeI CA'TA_TG Cuts at: 387 387 Size: 6229 NheI G'CTAG_C Cuts at: 1052 2899 1052 Size: 1847 4382 NotI GC'GGCC_GC Cuts at: 3676 3676 Size: 6229 NruI TCG'CGA Cuts at: 2042 2042 Size: 6229 NsiI A_TGCA'T Cuts at: 2343 2343 Size: 6229 NspI r_CATG'y Cuts at: 1132 1229 3587 5132 1132 Size: 97 2358 1545 2229 Fragments arranged by size: 2358 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6229 PciI A'CATG_T Cuts at: 5128 5128 Size: 6229 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 1108 Size: 902 5327 PmeI GTTT'AAAC Cuts at: 3533 3533 Size: 6229 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4298 4330 5811 5843 4298 Size: 32 1481 32 4684 Fragments arranged by size: 4684 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2584 2584 Size: 6229 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6229 PsiI TTA'TAA Cuts at: 2718 3132 3799 2718 Size: 414 667 5148 Fragments arranged by size: 5148 667 414 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2217 2249 2217 Size: 32 6197 PstI C_TGCA'G Cuts at: 830 2302 2581 3581 830 Size: 1472 279 1000 3478 Fragments arranged by size: 3478 1472 1000 279 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6229 SalI G'TCGA_C Cuts at: 1936 3571 1936 Size: 1635 4594 SbfI CC_TGCA'GG Cuts at: 3581 3581 Size: 6229 ScaI AGT'ACT Cuts at: 4477 6189 4477 Size: 1712 4517 SfcI C'TryA_G Cuts at: 826 1045 2201 2223 2298 2577 3577 4712 5393 Size: 219 1156 22 75 279 1000 1135 681 Cuts at: 5393 5584 826 Size: 191 1471 Fragments arranged by size: 1471 1156 1135 1000 681 279 219 191 75 22 SfoI GGC'GCC Cuts at: 2023 2023 Size: 6229 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6229 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6229 SmaI CCC'GGG Cuts at: 1872 3556 1872 Size: 1684 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2796 3471 4333 5234 5496 820 Size: 197 926 853 675 862 901 262 1553 Fragments arranged by size: 1553 926 901 862 853 675 262 197 SnaBI TAC'GTA Cuts at: 493 4020 493 Size: 3527 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6229 SphI G_CATG'C Cuts at: 1132 3587 1132 Size: 2455 3774 SspI AAT'ATT Cuts at: 5 52 4153 5 Size: 47 4101 2081 Fragments arranged by size: 4101 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6229 StyI C'CwwG_G Cuts at: 513 1065 2271 3668 513 Size: 552 1206 1397 3074 Fragments arranged by size: 3074 1397 1206 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2494 4256 4273 4426 4611 5056 325 Size: 1255 914 1762 17 153 185 445 1498 Fragments arranged by size: 1762 1498 1255 914 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3214 4475 6187 Size: 274 80 33 51 1443 1237 1261 1712 Cuts at: 6187 96 Size: 138 Fragments arranged by size: 1712 1443 1261 1237 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6189 6221 6189 Size: 32 6197 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6229 XbaI T'CTAG_A Cuts at: 3028 3565 3028 Size: 537 5692 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3523 3523 Size: 6229 XhoI C'TCGA_G Cuts at: 1943 2796 1943 Size: 853 5376 XmaI C'CCGG_G Cuts at: 1870 3554 1870 Size: 1684 4545 XmnI GAAnn'nnTTC Cuts at: 2293 4358 2293 Size: 2065 4164 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4037 276 Size: 53 83 186 936 2503 2468 Fragments arranged by size: 2503 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NruI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbvCI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV PacI PfoI PmlI PspOMI PspXI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI