Restriction map for
pFN21AE0329
(Linear) (Six-base) MAPSORT of: KIEE0329.seq Check: 7547 from: 1 to: 1524
KIEE0329.orf
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 70 1524
Size: 70 1454
AccI GT'mk_AC
Cuts at: 0 97 394 1524
Size: 97 297 1130
Fragments arranged by size:
1130 297 97
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1310 1524
Size: 1310 214
AfeI AGC'GCT
Cuts at: 0 1164 1524
Size: 1164 360
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1061 1524
Size: 1061 463
AlwNI CAG_nnn'CTG
Cuts at: 0 499 1524
Size: 499 1025
ApoI r'AATT_y
Cuts at: 0 260 314 962 1108 1448 1506 1524
Size: 260 54 648 146 340 58 18
Fragments arranged by size:
648 340 260 146 58 54 18
AseI AT'TA_AT
Cuts at: 0 1282 1524
Size: 1282 242
AvaI C'yCGr_G
Cuts at: 0 791 1524
Size: 791 733
BanI G'GyrC_C
Cuts at: 0 16 1524
Size: 16 1508
BbeI G_GCGC'C
Cuts at: 0 20 1524
Size: 20 1504
BbsI GAAGACnn'nnnn_
Cuts at: 0 54 967 1197 1524
Size: 54 913 230 327
Fragments arranged by size:
913 327 230 54
BfrBI ATG'CAT
Cuts at: 0 336 1524
Size: 336 1188
BglI GCCn_nnn'nGGC
Cuts at: 0 25 1524
Size: 25 1499
BmtI G_CTAG'C
Cuts at: 0 898 1524
Size: 898 626
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 857 1524
Size: 857 667
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 772 1296 1524
Size: 772 524 228
Fragments arranged by size:
772 524 228
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1451 1524
Size: 1451 73
BsaI GGTCTCn'nnnn_
Cuts at: 0 561 1524
Size: 561 963
BsaHI Gr'CG_yC
Cuts at: 0 17 1524
Size: 17 1507
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 524 696 1010 1524
Size: 524 172 314 514
Fragments arranged by size:
524 514 314 172
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 539 848 1031 1524
Size: 539 309 183 493
Fragments arranged by size:
539 493 309 183
BsiEI CG_ry'CG
Cuts at: 0 29 1524
Size: 29 1495
BsmI GAATG_Cn'
Cuts at: 0 752 1524
Size: 752 772
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1418 1524
Size: 1418 106
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 70 579 1524
Size: 70 509 945
Fragments arranged by size:
945 509 70
BsrBI CCG'CTC
Cuts at: 0 53 1524
Size: 53 1471
BsrGI T'GTAC_A
Cuts at: 0 1209 1524
Size: 1209 315
BstAPI GCAn_nnn'nTGC
Cuts at: 0 526 1524
Size: 526 998
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 18 1524
Size: 18 1506
ClaI AT'CG_AT
Cuts at: 0 173 1524
Size: 173 1351
DraI TTT'AAA
Cuts at: 0 1434 1524
Size: 1434 90
EaeI y'GGCC_r
Cuts at: 0 26 41 1524
Size: 26 15 1483
Fragments arranged by size:
1483 26 15
EagI C'GGCC_G
Cuts at: 0 26 1524
Size: 26 1498
EarI CTCTTCn'nnn_
Cuts at: 0 626 1022 1221 1524
Size: 626 396 199 303
Fragments arranged by size:
626 396 303 199
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1298 1524
Size: 1298 226
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 772 1296 1310 1524
Size: 772 524 14 214
Fragments arranged by size:
772 524 214 14
EcoO109I rG'GnC_Cy
Cuts at: 0 579 1524
Size: 579 945
EcoRI G'AATT_C
Cuts at: 0 260 314 962 1524
Size: 260 54 648 562
Fragments arranged by size:
648 562 260 54
HaeII r_GCGC'y
Cuts at: 0 20 1166 1524
Size: 20 1146 358
Fragments arranged by size:
1146 358 20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 79 111 475 507 1524
Size: 79 32 364 32 1017
Fragments arranged by size:
1017 364 79 32 32
KasI G'GCGC_C
Cuts at: 0 16 1524
Size: 16 1508
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 351 686 1524
Size: 351 335 838
Fragments arranged by size:
838 351 335
MslI CAynn'nnrTG
Cuts at: 0 592 1524
Size: 592 932
MspA1I CmG'CkG
Cuts at: 0 1223 1524
Size: 1223 301
NarI GG'CG_CC
Cuts at: 0 17 1524
Size: 17 1507
NheI G'CTAG_C
Cuts at: 0 894 1524
Size: 894 630
NruI TCG'CGA
Cuts at: 0 37 1524
Size: 37 1487
NsiI A_TGCA'T
Cuts at: 0 338 1524
Size: 338 1186
PpuMI rG'GwC_Cy
Cuts at: 0 579 1524
Size: 579 945
PsiI TTA'TAA
Cuts at: 0 713 1127 1524
Size: 713 414 397
Fragments arranged by size:
713 414 397
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 212 244 1524
Size: 212 32 1280
Fragments arranged by size:
1280 212 32
PstI C_TGCA'G
Cuts at: 0 297 576 1524
Size: 297 279 948
Fragments arranged by size:
948 297 279
SfcI C'TryA_G
Cuts at: 0 196 218 293 572 1524
Size: 196 22 75 279 952
Fragments arranged by size:
952 279 196 75 22
SfoI GGC'GCC
Cuts at: 0 18 1524
Size: 18 1506
SmlI C'TyrA_G
Cuts at: 0 791 1466 1524
Size: 791 675 58
Fragments arranged by size:
791 675 58
StyI C'CwwG_G
Cuts at: 0 266 1524
Size: 266 1258
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 489 1524
Size: 489 1035
TatI w'GTAC_w
Cuts at: 0 1209 1524
Size: 1209 315
XbaI T'CTAG_A
Cuts at: 0 1023 1524
Size: 1023 501
XhoI C'TCGA_G
Cuts at: 0 791 1524
Size: 791 733
XmnI GAAnn'nnTTC
Cuts at: 0 288 1524
Size: 288 1236
Enzymes that do cut:
AarI AccI AcuI AfeI AlfI AlwNI ApoI AseI
AvaI BanI BbeI BbsI BfrBI BglI BmtI BplI
BpmI BpuEI BsaI BsaHI BseRI BsgI BsiEI BsmI
BsmBI BspMI BsrBI BsrGI BstAPI BtgZI ClaI DraI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI HaeII
Hin4I KasI MmeI MslI MspA1I NarI NheI NruI
NsiI PpuMI PsiI PsrI PstI SfcI SfoI SmlI
StyI TaqII TatI XbaI XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AhdI AleI
AloI ApaI ApaLI AscI AsiSI AvrII BaeI BamHI
BanII BbvCI BcgI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI Bpu10I BsaAI BsaBI BsaWI BsaXI BseYI
BsiHKAI BsiWI Bsp1286I BspEI BspHI BsrDI BsrFI BssHII
BssSI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtsI CspCI DraIII DrdI EcoICRI EcoNI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KpnI MfeI
MluI MscI NaeI NcoI NdeI NgoMIV NotI NspI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PspOMI PspXI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI TstI Tth111I XcmI XmaI ZraI