Restriction map for
pFN21AE1467
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# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflII AflIII AgeI AhdI
AlwNI Aor51HI AscI Asp718I AspEI
AspI AviII AvrII BamHI BbeI
BbrPI BcgI BfaI BfrI BglI
BlnI BlpI Bpu1102I BsaBI BsaHI
BsaI BsgI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspHI BspLU11I BspMI
BsrGI BssHII BssSI Bst1107I BstAPI
BstEII BstPI BstUI BstXI BstZ17I
CelII CfoI ClaI CpoI DraI
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoO65I EcoRV EcoT22I
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HindIII HpaI KasI
KpnI Ksp632I KspI MaeI MamI
MluI MspA1I MvnI NarI NdeI
NheI NotI NruI NsiI PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SwaI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccIII 590 TCCGGA T^CCGGA
Alw44I 1477 GTGCAC G^TGCAC
AlwI 403 GATCC GGATC(4/5)
ApaLI 1477 GTGCAC G^TGCAC
AseI 561 ATTAAT AT^TAAT
AsnI 561 ATTAAT AT^TAAT
AspHI 1477 GTGCAC G[AT]GC[AT]^C
BalI 155 TGGCCA TGG^CCA
BanI 1010 GGCACC G^G[CT][GA]CC
BbvCI 1419 CCTCAGC CCTCAGC(-5/-2)
BciVI 1055 GTATCC GTATCC(6/5)
BclI 1502 TGATCA T^GATCA
BsaAI 683 TACGTG [CT]AC^GT[GA]
BsaWI 590 TCCGGA [AT]^CCGG[AT]
BseAI 590 TCCGGA T^CCGGA
BseRI 1489 GAGGAG GAGGAG(10/8)
BsiHKAI 1477 GTGCAC G[AT]GC[AT]^C
BspEI 590 TCCGGA T^CCGGA
BsrBI 1223 GAGCGG CCGCTC(-3/-3)
BsrDI 348 CATTGC GCAATG(2/0)
BsrFI 1411 GCCGGC [GA]^CCGG[CT]
BstBI 269 TTCGAA TT^CGAA
Cfr10I 1411 GCCGGC [GA]^CCGG[CT]
DraIII 546 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 663 CCATGG C^C[GA][CT]GG
EaeI 155 TGGCCA [CT]^GGCC[GA]
EcoRI 1555 GAATTC G^AATTC
FauI 1225 GCGGG CCCGC(4/6)
FbaI 1502 TGATCA T^GATCA
HincII 1574 GTCAAC GT[CT]^[GA]AC
HindII 1574 GTCAAC GT[CT]^[GA]AC
MfeI 1565 CAATTG C^AATTG
MluNI 155 TGGCCA TGG^CCA
MroI 590 TCCGGA T^CCGGA
MscI 155 TGGCCA TGG^CCA
MunI 1565 CAATTG C^AATTG
NaeI 1411 GCCGGC GCC^GGC
NcoI 663 CCATGG C^CATGG
NgoMIV 1411 GCCGGC G^CCGGC
NspV 269 TTCGAA TT^CGAA
PpuMI 822 GGGTCCC [GA]G^G[AT]CC[CT]
PshBI 561 ATTAAT AT^TAAT
PstI 167 CTGCAG CTGCA^G
SfuI 269 TTCGAA TT^CGAA
SmlI 1258 CTCAAG C^T[CT][GA]AG
SphI 191 GCATGC GCATG^C
SspI 1277 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcsI 180 GAATTT [GA]^AATT[CT]
1555 GAATTC
AocI 234 CCTCAGG CC^T[ACGT]AGG
1036 CCTGAGG
ApaI 161 GGGCCC GGGCC^C
1165 GGGCCC
ApoI 180 GAATTT [GA]^AATT[CT]
1555 GAATTC
Asp700I 63 GAACGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
942 GAAGGGGTTC
AvaI 386 CCCGAG C^[CT]CG[GA]G
1673 CTCGGG
AvaII 823 GGTCC G^G[AT]CC
1612 GGACC
BcnI 90 CCGGG CC^[GC]GG
1335 CCGGG
BglII 1313 AGATCT A^GATCT
1365 AGATCT
BsmAI 434 GAGAC GTCTC(1/5)
1455 GTCTC
BsmI 381 GAATGC GAATGC(1/-1)
766 GAATGC
BsoBI 386 CCCGAG C^[CT]CG[GA]G
1673 CTCGGG
BstNI 158 CCAGG CC^[AT]GG
504 CCTGG
BstYI 1313 AGATCT [GA]^GATC[CT]
1365 AGATCT
Bsu36I 234 CCTCAGG CC^T[ACGT]AGG
1036 CCTGAGG
Eco81I 234 CCTCAGG CC^T[ACGT]AGG
1036 CCTGAGG
EcoNI 230 CCTTCCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
487 CCTGAAGGAGG
EcoRII 158 CCAGG ^CC[AT]GG
504 CCTGG
MaeII 65 ACGT A^CGT
684 ACGT
MflI 1313 AGATCT [GA]^GATC[CT]
1365 AGATCT
MseI 362 TTAA T^TAA
562 TTAA
MslI 891 CACCAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1480 CACCATGATG
MvaI 158 CCAGG CC^[AT]GG
504 CCTGG
NciI 90 CCGGG CC^[GC]GG
1335 CCGGG
NspI 191 GCATGC [GA]CATG^[CT]
1344 ACATGC
SfcI 167 CTGCAG C^T[GA][CT]AG
928 CTACAG
StuI 495 AGGCCT AGG^CCT
1509 AGGCCT
Tru9I 362 TTAA T^TAA
562 TTAA
XhoII 1313 AGATCT [GA]^GATC[CT]
1365 AGATCT
XmnI 63 GAACGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
942 GAAGGGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AfaI AluI BanII BbsI
BbvI BmyI BpmI BpuAI BsaJI
BsiYI BslI BsmFI Bsp1286I BsrI
BssKI BstF5I Cac8I Cfr13I DdeI
DpnII DraII EcoO109I EcoT14I Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI MaeIII MboI MboII
MnlI MspI MwoI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I