(Circular) (Six-base) MAPSORT of: pFN21AE1467.seq Check: 8789 from: 1 to: 6415 pFN21AE1467 6415 bp With 182 enzymes: SgfI * January 31, 2011 17:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4225 278 Size: 53 83 186 936 2689 2468 Fragments arranged by size: 2689 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3758 1937 Size: 1821 4594 Acc65I G'GTAC_C Cuts at: 3736 3736 Size: 6415 AclI AA'CG_TT Cuts at: 1563 4542 4915 1563 Size: 2979 373 3063 Fragments arranged by size: 3063 2979 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2514 3326 3642 4478 5862 6130 1632 Size: 882 812 316 836 1384 268 1917 Fragments arranged by size: 1917 1384 882 836 812 316 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6218 AflIII A'CryG_T Cuts at: 1203 5314 6291 1203 Size: 4111 977 1327 Fragments arranged by size: 4111 1327 977 AgeI A'CCGG_T Cuts at: 5246 5246 Size: 6415 AhdI GACnn_n'nnGTC Cuts at: 5144 5144 Size: 6415 AleI CACnn'nnGTG Cuts at: 1166 2900 1166 Size: 1734 4681 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6415 AlwNI CAG_nnn'CTG Cuts at: 4158 5730 4158 Size: 1572 4843 ApaI G_GGCC'C Cuts at: 2170 3174 2170 Size: 1004 5411 ApaLI G'TGCA_C Cuts at: 3482 4472 5628 3482 Size: 990 1156 4269 Fragments arranged by size: 4269 1156 990 ApoI r'AATT_y Cuts at: 1493 2185 3560 3724 3950 4102 6211 1493 Size: 692 1375 164 226 152 2109 1697 Fragments arranged by size: 2109 1697 1375 692 226 164 152 AseI AT'TA_AT Cuts at: 160 1032 2567 4969 160 Size: 872 1535 2402 1606 Fragments arranged by size: 2402 1606 1535 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6415 AvaI C'yCGr_G Cuts at: 1870 1943 2391 3678 3740 1870 Size: 73 448 1287 62 4545 Fragments arranged by size: 4545 1287 448 73 62 BamHI G'GATC_C Cuts at: 3745 4112 3745 Size: 367 6048 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3015 3736 5191 618 Size: 325 217 627 1228 721 1455 1842 Fragments arranged by size: 1842 1455 1228 721 627 325 217 BanII G_rGCy'C Cuts at: 1391 2034 2170 3174 3734 1391 Size: 643 136 1004 560 4072 Fragments arranged by size: 4072 1004 643 560 136 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2065 2284 3054 928 Size: 971 166 219 770 4289 Fragments arranged by size: 4289 971 770 219 166 BbvCI CC'TCA_GC Cuts at: 3425 3425 Size: 6415 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4606 4640 1903 Size: 34 2669 34 3678 Fragments arranged by size: 3678 2669 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3071 4307 5517 3071 Size: 1236 1210 3969 Fragments arranged by size: 3969 1236 1210 BclI T'GATC_A Cuts at: 1548 3507 1548 Size: 1959 4456 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5026 136 Size: 107 122 71 631 605 3354 1525 Fragments arranged by size: 3354 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3318 3370 6410 3318 Size: 52 3040 3323 Fragments arranged by size: 3323 3040 52 BlpI GC'TnA_GC Cuts at: 3832 3832 Size: 6415 Bme1580I G_kGCm'C Cuts at: 1790 2170 2993 3174 3486 4476 5632 1790 Size: 380 823 181 312 990 1156 2573 Fragments arranged by size: 2573 1156 990 823 380 312 181 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6415 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2023 3559 4146 Size: 416 53 83 399 24 606 1536 587 Cuts at: 4146 5104 442 Size: 958 1753 Fragments arranged by size: 1753 1536 958 606 587 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6415 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2050 3626 2050 Size: 1576 4839 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2559 3029 3407 5075 1239 Size: 1320 470 378 1668 2579 Fragments arranged by size: 2579 1668 1320 470 378 Bpu10I CC'TnA_GC Cuts at: 3425 3425 Size: 6415 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3248 4540 5405 5703 3248 Size: 1292 865 298 3960 Fragments arranged by size: 3960 1292 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5078 882 Size: 617 3579 2219 Fragments arranged by size: 3579 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2690 4206 493 Size: 711 1486 1516 2702 Fragments arranged by size: 2702 1516 1486 711 BsaBI GATnn'nnATC Cuts at: 6409 6409 Size: 6415 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4222 4604 5239 275 Size: 53 83 186 936 2689 382 635 1451 Fragments arranged by size: 2689 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2595 4848 5246 5520 5667 6078 2595 Size: 2253 398 274 147 411 2932 Fragments arranged by size: 2932 2253 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6190 Fragments arranged by size: 6190 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3509 1186 Size: 2323 4092 BseYI C'CCAG_C Cuts at: 2059 3450 5618 6114 2059 Size: 1391 2168 496 2360 Fragments arranged by size: 2360 2168 1391 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5842 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3865 4626 5654 1540 Size: 275 186 1864 761 1028 2301 Fragments arranged by size: 2301 1864 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3486 4476 4561 5632 3486 Size: 990 85 1071 4269 Fragments arranged by size: 4269 1071 990 85 BsmI GAATG_Cn' Cuts at: 2392 2777 3926 4019 2392 Size: 385 1149 93 4788 Fragments arranged by size: 4788 1149 385 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2034 2170 2993 3174 3486 3734 4476 Size: 399 244 136 823 181 312 248 742 Cuts at: 4476 4561 5632 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 823 742 399 312 248 244 181 136 85 BspEI T'CCGG_A Cuts at: 2595 6078 2595 Size: 3483 2932 BspHI T'CATG_A Cuts at: 4304 6034 4304 Size: 1730 4685 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3770 844 Size: 2926 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3230 4302 1394 Size: 429 1407 1072 3507 Fragments arranged by size: 3507 1407 1072 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2352 4910 5084 65 Size: 1183 1104 2558 174 1396 Fragments arranged by size: 2558 1396 1183 1104 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3416 5059 5246 1700 Size: 110 1606 1643 187 2869 Fragments arranged by size: 2869 1643 1606 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6415 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6415 BssSI C'ACGA_G Cuts at: 4475 5487 4475 Size: 1012 5403 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6415 BstBI TT'CG_AA Cuts at: 2275 5219 2275 Size: 2944 3471 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6415 BstYI r'GATC_y Cuts at: 1972 3318 3370 3745 4112 4497 4514 5966 6052 Size: 1346 52 375 367 385 17 1452 86 Cuts at: 6052 6064 6410 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1346 385 375 367 346 86 52 17 12 Bsu36I CC'TnA_GG Cuts at: 2240 3042 2240 Size: 802 5613 BtgI C'CryG_G Cuts at: 513 1065 2004 2668 513 Size: 552 939 664 4260 Fragments arranged by size: 4260 939 664 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5676 Fragments arranged by size: 5676 683 56 BtsI GCAGTG_nn' Cuts at: 3180 3931 4725 4745 3180 Size: 751 794 20 4850 Fragments arranged by size: 4850 794 751 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6415 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3091 3122 3126 3157 529 Size: 35 2527 31 4 31 3787 Fragments arranged by size: 3787 2527 35 31 31 4 DraI TTT'AAA Cuts at: 3719 3719 Size: 6415 DraIII CAC_nnn'GTG Cuts at: 2556 2556 Size: 6415 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5422 809 Size: 507 4106 1802 Fragments arranged by size: 4106 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2160 3862 4751 Size: 54 1425 50 275 18 330 1702 889 Cuts at: 4751 6380 8 Size: 1629 43 Fragments arranged by size: 1702 1629 1425 889 330 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3862 1537 Size: 275 2050 4090 Fragments arranged by size: 4090 2050 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4345 1353 Size: 2992 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3025 5002 5376 5522 387 Size: 1064 138 1436 1977 374 146 1280 Fragments arranged by size: 1977 1436 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2514 2559 3029 3326 3407 3642 4478 Size: 393 882 45 470 297 81 235 836 Cuts at: 4478 5075 5862 6130 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 882 836 787 597 470 393 297 268 235 81 45 EcoNI CCTnn'n_nnAGG Cuts at: 2239 2496 2239 Size: 257 6158 EcoO109I rG'GnC_Cy Cuts at: 2166 2167 2828 3171 2166 Size: 1 661 343 5410 Fragments arranged by size: 5410 661 343 1 EcoRI G'AATT_C Cuts at: 3560 3724 4102 3560 Size: 164 378 5873 Fragments arranged by size: 5873 378 164 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3340 3727 3340 Size: 387 6028 FspI TGC'GCA Cuts at: 4921 4921 Size: 6415 HaeII r_GCGC'y Cuts at: 1678 5562 1678 Size: 3884 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2532 2564 2812 2844 5056 Size: 32 135 32 960 32 248 32 2212 Cuts at: 5056 5088 5130 5162 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2212 960 248 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3581 3759 4005 669 Size: 988 281 1643 178 246 3079 Fragments arranged by size: 3079 1643 988 281 246 178 HindIII A'AGCT_T Cuts at: 748 748 Size: 6415 HpaI GTT'AAC Cuts at: 4005 4005 Size: 6415 KpnI G_GTAC'C Cuts at: 3740 3740 Size: 6415 MfeI C'AATT_G Cuts at: 3570 4014 3570 Size: 444 5971 MluI A'CGCG_T Cuts at: 6291 6291 Size: 6415 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3684 5529 5713 1305 Size: 2379 1845 184 2007 Fragments arranged by size: 2379 2007 1845 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2162 10 Size: 54 1425 343 330 4263 Fragments arranged by size: 4263 1425 343 330 54 MslI CAynn'nnrTG Cuts at: 518 1166 2900 3489 4373 4732 4891 518 Size: 648 1734 589 884 359 159 2042 Fragments arranged by size: 2042 1734 884 648 589 359 159 MspA1I CmG'CkG Cuts at: 1606 3831 4508 5656 5901 1606 Size: 2225 677 1148 245 2120 Fragments arranged by size: 2225 2120 1148 677 245 NaeI GCC'GGC Cuts at: 3418 3418 Size: 6415 NcoI C'CATG_G Cuts at: 513 1065 2004 2668 513 Size: 552 939 664 4260 Fragments arranged by size: 4260 939 664 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6415 NgoMIV G'CCGG_C Cuts at: 3416 3416 Size: 6415 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6415 NotI GC'GGCC_GC Cuts at: 3862 3862 Size: 6415 NspI r_CATG'y Cuts at: 1132 1229 2200 3353 3773 5318 1132 Size: 97 971 1153 420 1545 2229 Fragments arranged by size: 2229 1545 1153 971 420 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6415 PciI A'CATG_T Cuts at: 5314 5314 Size: 6415 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6415 PmeI GTTT'AAAC Cuts at: 3719 3719 Size: 6415 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3083 3115 4484 4516 5997 6029 3083 Size: 32 1369 32 1481 32 3469 Fragments arranged by size: 3469 1481 1369 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2828 2828 Size: 6415 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6415 PsiI TTA'TAA Cuts at: 3985 3985 Size: 6415 PspOMI G'GGCC_C Cuts at: 2166 3170 2166 Size: 1004 5411 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2670 2702 2670 Size: 32 6383 PstI C_TGCA'G Cuts at: 830 2176 3767 830 Size: 1346 1591 3478 Fragments arranged by size: 3478 1591 1346 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6415 SalI G'TCGA_C Cuts at: 1936 3757 1936 Size: 1821 4594 SanDI GG'GwC_CC Cuts at: 2828 2828 Size: 6415 SbfI CC_TGCA'GG Cuts at: 3767 3767 Size: 6415 ScaI AGT'ACT Cuts at: 4663 6375 4663 Size: 1712 4703 SfcI C'TryA_G Cuts at: 826 1045 2172 2933 3763 4898 5579 5770 826 Size: 219 1127 761 830 1135 681 191 1471 Fragments arranged by size: 1471 1135 1127 830 761 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6415 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6415 SmaI CCC'GGG Cuts at: 1872 3742 1872 Size: 1870 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3263 4519 5420 5682 820 Size: 197 926 1320 1256 901 262 1553 Fragments arranged by size: 1553 1320 1256 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4206 493 Size: 3713 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6415 SphI G_CATG'C Cuts at: 1132 2200 3773 1132 Size: 1068 1573 3774 Fragments arranged by size: 3774 1573 1068 SspI AAT'ATT Cuts at: 5 52 3284 4339 5 Size: 47 3232 1055 2081 Fragments arranged by size: 3232 2081 1055 47 StuI AGG'CCT Cuts at: 1517 2502 3516 1517 Size: 985 1014 4416 Fragments arranged by size: 4416 1014 985 StyI C'CwwG_G Cuts at: 513 1065 2004 2668 2898 3627 3854 513 Size: 552 939 664 230 729 227 3074 Fragments arranged by size: 3074 939 729 664 552 230 227 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4442 4459 4612 4797 5242 325 Size: 1255 2862 17 153 185 445 1498 Fragments arranged by size: 2862 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4661 6373 96 Size: 274 80 33 51 1443 2684 1712 138 Fragments arranged by size: 2684 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6375 6407 6375 Size: 32 6383 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6415 XbaI T'CTAG_A Cuts at: 3751 3751 Size: 6415 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6415 XmaI C'CCGG_G Cuts at: 1870 3740 1870 Size: 1870 4545 XmnI GAAnn'nnTTC Cuts at: 2072 2951 4544 2072 Size: 879 1593 3943 Fragments arranged by size: 3943 1593 879 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4223 276 Size: 53 83 186 936 2689 2468 Fragments arranged by size: 2689 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PvuII RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI