(Circular) (Six-base) MAPSORT of: pFN21AE0311.seq Check: 3033 from: 1 to: 8500 pFN21AE0311 8500 bp With 182 enzymes: SgfI * April 15, 2010 09:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4312 4312 Size: 8500 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3696 5234 6310 278 Size: 53 83 186 936 2160 1538 1076 2468 Fragments arranged by size: 2468 2160 1538 1076 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5324 5843 1937 Size: 3387 519 4594 Fragments arranged by size: 4594 3387 519 Acc65I G'GTAC_C Cuts at: 5821 5821 Size: 8500 AclI AA'CG_TT Cuts at: 1563 6627 7000 1563 Size: 5064 373 3063 Fragments arranged by size: 5064 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2559 4197 5706 6563 7947 8215 1632 Size: 927 1638 1509 857 1384 268 1917 Fragments arranged by size: 1917 1638 1509 1384 927 857 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8303 AflIII A'CryG_T Cuts at: 1203 3888 4798 7399 8376 1203 Size: 2685 910 2601 977 1327 Fragments arranged by size: 2685 2601 1327 977 910 AgeI A'CCGG_T Cuts at: 7331 7331 Size: 8500 AhdI GACnn_n'nnGTC Cuts at: 7229 7229 Size: 8500 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8500 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2474 1184 Size: 1290 7210 AlwNI CAG_nnn'CTG Cuts at: 5099 6243 7815 5099 Size: 1144 1572 5784 Fragments arranged by size: 5784 1572 1144 ApaLI G'TGCA_C Cuts at: 6557 7713 6557 Size: 1156 7344 ApoI r'AATT_y Cuts at: 1493 2076 2215 2272 3539 3557 3574 3704 3742 Size: 583 139 57 1267 18 17 130 38 Cuts at: 3742 4586 4624 4929 5527 5597 5809 6035 6187 Size: 844 38 305 598 70 212 226 152 Cuts at: 6187 8296 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1267 844 598 583 305 226 212 152 139 130 70 57 38 38 18 17 AseI AT'TA_AT Cuts at: 160 1032 3831 7054 160 Size: 872 2799 3223 1606 Fragments arranged by size: 3223 2799 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8500 AvaI C'yCGr_G Cuts at: 1870 1943 5825 1870 Size: 73 3882 4545 Fragments arranged by size: 4545 3882 73 BamHI G'GATC_C Cuts at: 5830 6197 5830 Size: 367 8133 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5821 7276 618 Size: 325 217 627 4034 1455 1842 Fragments arranged by size: 4034 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 5819 1391 Size: 4428 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2456 3866 4166 4287 4748 5258 928 Size: 971 557 1410 300 121 461 510 4170 Fragments arranged by size: 4170 1410 971 557 510 461 300 121 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3112 3146 6691 6725 1903 Size: 34 1175 34 3545 34 3678 Fragments arranged by size: 3678 3545 1175 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6392 7602 6392 Size: 1210 7290 BclI T'GATC_A Cuts at: 1548 4924 1548 Size: 3376 5124 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 7111 136 Size: 107 122 71 631 605 5439 1525 Fragments arranged by size: 5439 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3308 4822 5167 5414 5713 8495 3308 Size: 1514 345 247 299 2782 3313 Fragments arranged by size: 3313 2782 1514 345 299 247 BlpI GC'TnA_GC Cuts at: 4692 5917 4692 Size: 1225 7275 Bme1580I G_kGCm'C Cuts at: 1790 2015 6561 7717 1790 Size: 225 4546 1156 2573 Fragments arranged by size: 4546 2573 1156 225 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8500 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2009 2764 5108 Size: 416 53 83 399 24 592 755 2344 Cuts at: 5108 6231 7189 442 Size: 1123 958 1753 Fragments arranged by size: 2344 1753 1123 958 755 592 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2424 1056 Size: 1368 7132 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5256 7160 1239 Size: 4017 1904 2579 Fragments arranged by size: 4017 2579 1904 Bpu10I CC'TnA_GC Cuts at: 2190 3399 4139 4721 2190 Size: 1209 740 582 5969 Fragments arranged by size: 5969 1209 740 582 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 6625 7490 7788 6625 Size: 865 298 7337 Fragments arranged by size: 7337 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 7163 882 Size: 617 5664 2219 Fragments arranged by size: 5664 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 6291 493 Size: 711 5087 2702 Fragments arranged by size: 5087 2702 711 BsaBI GATnn'nnATC Cuts at: 5100 8494 5100 Size: 3394 5106 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3693 5231 6307 6689 Size: 53 83 186 936 2160 1538 1076 382 Cuts at: 6689 7324 275 Size: 635 1451 Fragments arranged by size: 2160 1538 1451 1076 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3855 5610 6933 7331 7605 7752 8163 3855 Size: 1755 1323 398 274 147 411 4192 Fragments arranged by size: 4192 1755 1323 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 8275 Fragments arranged by size: 8275 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 8500 BseYI C'CCAG_C Cuts at: 4012 5548 7703 8199 4012 Size: 1536 2155 496 4313 Fragments arranged by size: 4313 2155 1536 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3357 1163 Size: 573 1621 6306 Fragments arranged by size: 6306 1621 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5950 6711 7739 1540 Size: 275 186 3949 761 1028 2301 Fragments arranged by size: 3949 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5546 6561 6646 7717 5546 Size: 1015 85 1071 6329 Fragments arranged by size: 6329 1071 1015 85 BsmI GAATG_Cn' Cuts at: 4868 6011 6104 4868 Size: 1143 93 7264 Fragments arranged by size: 7264 1143 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2015 5546 5819 6561 6646 7717 1391 Size: 399 225 3531 273 742 85 1071 2174 Fragments arranged by size: 3531 2174 1071 742 399 273 225 85 BspEI T'CCGG_A Cuts at: 8163 8163 Size: 8500 BspHI T'CATG_A Cuts at: 2343 6389 8119 2343 Size: 4046 1730 2724 Fragments arranged by size: 4046 2724 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4312 4399 5855 844 Size: 3468 87 1456 3489 Fragments arranged by size: 3489 3468 1456 87 BsrBI CCG'CTC Cuts at: 1394 1823 6387 1394 Size: 429 4564 3507 Fragments arranged by size: 4564 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6995 7169 65 Size: 1183 5747 174 1396 Fragments arranged by size: 5747 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 7144 7331 1700 Size: 110 5334 187 2869 Fragments arranged by size: 5334 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 4501 96 Size: 4405 4095 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8500 BssSI C'ACGA_G Cuts at: 6560 7572 6560 Size: 1012 7488 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8500 BstBI TT'CG_AA Cuts at: 7304 7304 Size: 8500 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8500 BstXI CCAn_nnnn'nTGG Cuts at: 2777 2777 Size: 8500 BstYI r'GATC_y Cuts at: 1972 2986 3308 3343 4822 5167 5263 5414 5713 Size: 1014 322 35 1479 345 96 151 299 Cuts at: 5713 5830 6197 6582 6599 8051 8137 8149 8495 Size: 117 367 385 17 1452 86 12 346 Cuts at: 8495 1972 Size: 1977 Fragments arranged by size: 1977 1479 1452 1014 385 367 346 345 322 299 151 117 96 86 35 17 12 Bsu36I CC'TnA_GG Cuts at: 2044 2044 Size: 8500 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 7009 Fragments arranged by size: 7009 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7761 Fragments arranged by size: 7761 683 56 BtsI GCAGTG_nn' Cuts at: 2534 5685 6016 6810 6830 2534 Size: 3151 331 794 20 4204 Fragments arranged by size: 4204 3151 794 331 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8500 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4306 4341 529 Size: 35 3742 35 4688 Fragments arranged by size: 4688 3742 35 35 DraI TTT'AAA Cuts at: 2166 3256 4834 5073 5602 5767 5804 2166 Size: 1090 1578 239 529 165 37 4862 Fragments arranged by size: 4862 1578 1090 529 239 165 37 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5236 7507 809 Size: 507 3920 2271 1802 Fragments arranged by size: 3920 2271 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2779 4375 5947 Size: 54 1425 50 275 18 949 1596 1572 Cuts at: 5947 6836 8465 8 Size: 889 1629 43 Fragments arranged by size: 1629 1596 1572 1425 949 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5947 1537 Size: 275 4135 4090 Fragments arranged by size: 4135 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2578 5791 6430 1353 Size: 1225 3213 639 3423 Fragments arranged by size: 3423 3213 1225 639 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7087 7461 7607 387 Size: 1064 138 5498 374 146 1280 Fragments arranged by size: 5498 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2559 4197 5256 5706 6563 7160 7947 Size: 393 927 1638 1059 450 857 597 787 Cuts at: 7947 8215 1239 Size: 268 1524 Fragments arranged by size: 1638 1524 1059 927 857 787 597 450 393 268 EcoO109I rG'GnC_Cy Cuts at: 3759 4056 3759 Size: 297 8203 EcoRI G'AATT_C Cuts at: 2215 3574 5809 6187 2215 Size: 1359 2235 378 4528 Fragments arranged by size: 4528 2235 1359 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3618 5410 3618 Size: 1792 6708 FspI TGC'GCA Cuts at: 7006 7006 Size: 8500 HaeII r_GCGC'y Cuts at: 1678 7647 1678 Size: 5969 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2555 2587 2783 2815 2926 Size: 32 135 32 983 32 196 32 111 Cuts at: 2926 2958 3203 3235 3300 3332 4670 4702 7141 Size: 32 245 32 65 32 1338 32 2439 Cuts at: 7141 7173 7215 7247 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2439 1338 983 245 196 135 111 65 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4900 5031 5608 5844 6090 669 Size: 988 281 2962 131 577 236 246 3079 Fragments arranged by size: 3079 2962 988 577 281 246 236 131 HindIII A'AGCT_T Cuts at: 748 2321 3600 3836 4136 748 Size: 1573 1279 236 300 5112 Fragments arranged by size: 5112 1573 1279 300 236 HpaI GTT'AAC Cuts at: 5031 6090 5031 Size: 1059 7441 KpnI G_GTAC'C Cuts at: 5825 5825 Size: 8500 MfeI C'AATT_G Cuts at: 3068 6099 3068 Size: 3031 5469 MluI A'CGCG_T Cuts at: 8376 8376 Size: 8500 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 7614 7798 1305 Size: 6309 184 2007 Fragments arranged by size: 6309 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4377 10 Size: 54 1425 343 2545 4133 Fragments arranged by size: 4133 2545 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2949 3891 4094 4377 6458 6817 6976 Size: 648 1783 942 203 283 2081 359 159 Cuts at: 6976 518 Size: 2042 Fragments arranged by size: 2081 2042 1783 942 648 359 283 203 159 MspA1I CmG'CkG Cuts at: 1606 3373 3394 5916 6593 7741 7986 1606 Size: 1767 21 2522 677 1148 245 2120 Fragments arranged by size: 2522 2120 1767 1148 677 245 21 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 7009 Fragments arranged by size: 7009 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8500 NheI G'CTAG_C Cuts at: 1052 2420 1052 Size: 1368 7132 NotI GC'GGCC_GC Cuts at: 5947 5947 Size: 8500 NspI r_CATG'y Cuts at: 1132 1229 3892 4802 5858 7403 1132 Size: 97 2663 910 1056 1545 2229 Fragments arranged by size: 2663 2229 1545 1056 910 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8500 PciI A'CATG_T Cuts at: 3888 4798 7399 3888 Size: 910 2601 4989 Fragments arranged by size: 4989 2601 910 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 8500 PfoI T'CCnGG_A Cuts at: 3318 5104 3318 Size: 1786 6714 PmeI GTTT'AAAC Cuts at: 5804 5804 Size: 8500 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6569 6601 8082 8114 6569 Size: 32 1481 32 6955 Fragments arranged by size: 6955 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3759 4056 3759 Size: 297 8203 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8500 PsiI TTA'TAA Cuts at: 6070 6070 Size: 8500 PstI C_TGCA'G Cuts at: 830 2866 5852 830 Size: 2036 2986 3478 Fragments arranged by size: 3478 2986 2036 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8500 PvuII CAG'CTG Cuts at: 3373 3394 3373 Size: 21 8479 SalI G'TCGA_C Cuts at: 1936 5842 1936 Size: 3906 4594 SanDI GG'GwC_CC Cuts at: 3759 3759 Size: 8500 SbfI CC_TGCA'GG Cuts at: 5852 5852 Size: 8500 ScaI AGT'ACT Cuts at: 6748 8460 6748 Size: 1712 6788 SfcI C'TryA_G Cuts at: 826 1045 2685 2862 4051 5848 6983 7664 7855 Size: 219 1640 177 1189 1797 1135 681 191 Cuts at: 7855 826 Size: 1471 Fragments arranged by size: 1797 1640 1471 1189 1135 681 219 191 177 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8500 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8500 SmaI CCC'GGG Cuts at: 1872 5827 1872 Size: 3955 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 6604 7505 7767 820 Size: 197 926 4661 901 262 1553 Fragments arranged by size: 4661 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 6291 493 Size: 5798 2702 SpeI A'CTAG_T Cuts at: 152 5084 152 Size: 4932 3568 SphI G_CATG'C Cuts at: 1132 5858 1132 Size: 4726 3774 SspI AAT'ATT Cuts at: 5 52 2542 6424 5 Size: 47 2490 3882 2081 Fragments arranged by size: 3882 2490 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8500 StyI C'CwwG_G Cuts at: 513 1065 2004 5535 5939 513 Size: 552 939 3531 404 3074 Fragments arranged by size: 3531 3074 939 552 404 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4658 6527 6544 6697 6882 7327 325 Size: 1255 3078 1869 17 153 185 445 1498 Fragments arranged by size: 3078 1869 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3463 4501 5750 Size: 274 80 33 51 1443 1486 1038 1249 Cuts at: 5750 6746 8458 96 Size: 996 1712 138 Fragments arranged by size: 1712 1486 1443 1249 1038 996 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2815 2847 8460 8492 2815 Size: 32 5613 32 2823 Fragments arranged by size: 5613 2823 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8500 XbaI T'CTAG_A Cuts at: 3605 5836 3605 Size: 2231 6269 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8500 XmaI C'CCGG_G Cuts at: 1870 5825 1870 Size: 3955 4545 XmnI GAAnn'nnTTC Cuts at: 3194 4458 6629 3194 Size: 1264 2171 5065 Fragments arranged by size: 5065 2171 1264 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3694 5232 6308 276 Size: 53 83 186 936 2160 1538 1076 2468 Fragments arranged by size: 2468 2160 1538 1076 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI